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==Swapping mitochondrial DNA in mammalian oocytes== | ==Swapping mitochondrial DNA in mammalian oocytes== | ||
Technique has the potential to prevent transmission of human mutated mitochondrial DNA from mother to baby. (figure reference | Technique has the potential to prevent transmission of human mutated mitochondrial DNA from mother to baby. (figure reference{{#pmid:19759608|PMID19759608}}) | ||
Working with rhesus macaque monkeys, Tachibana et al. | Working with rhesus macaque monkeys, Tachibana et al.{{#pmid:19710649|PMID19710649}}: | ||
* '''a''' - removed the nuclear material plus a cellular membrane (a karyoplast) from a mature oocyte, leaving behind its mitochondria. The nuclear material in the karyoplast consists of condensed chromosomes attached to thread-like spindle fibres (the spindle–chromosomal complex). | * '''a''' - removed the nuclear material plus a cellular membrane (a karyoplast) from a mature oocyte, leaving behind its mitochondria. The nuclear material in the karyoplast consists of condensed chromosomes attached to thread-like spindle fibres (the spindle–chromosomal complex). | ||
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:'''Links:''' | :'''Links:''' {{mitochondria}} | {{Assisted Reproductive Technology}} | ||
===Reference=== | ===Reference=== | ||
Latest revision as of 11:25, 7 May 2018
Swapping mitochondrial DNA in mammalian oocytes
Technique has the potential to prevent transmission of human mutated mitochondrial DNA from mother to baby. (figure reference[1])
Working with rhesus macaque monkeys, Tachibana et al.[2]:
- a - removed the nuclear material plus a cellular membrane (a karyoplast) from a mature oocyte, leaving behind its mitochondria. The nuclear material in the karyoplast consists of condensed chromosomes attached to thread-like spindle fibres (the spindle–chromosomal complex).
- b - transferred the karyoplast to an oocyte whose nucleus had been removed (a cytoplast).
- c - fused the karyoplast with the cytoplast and then fertilized the reconstructed oocyte.
- d - developing blastocyst was implanted in a surrogate mother.
- e - mother gave birth to a healthy baby.
Reference
- ↑ Shoubridge EA. (2009). Developmental biology: Asexual healing. Nature , 461, 354-5. PMID: 19759608 DOI.
- ↑ Tachibana M, Sparman M, Sritanaudomchai H, Ma H, Clepper L, Woodward J, Li Y, Ramsey C, Kolotushkina O & Mitalipov S. (2009). Mitochondrial gene replacement in primate offspring and embryonic stem cells. Nature , 461, 367-72. PMID: 19710649 DOI.
Nature 461, 354-355 (17 September 2009) | doi:10.1038/461354a; Published online 16 September 2009
Original Image Link: http://www.nature.com/nature/journal/v461/n7262/fig_tab/461354a_F1.html
Cite this page: Hill, M.A. (2024, April 23) Embryology Swapping mitochondrial DNA mammalian oocytes.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Swapping_mitochondrial_DNA_mammalian_oocytes.jpg
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G
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