|Schematic representation of the organization of the spleen (left panel).
- The white pulp consists of T cell (lymphocyte) zones (also known as the periarteriolar lymphoid sheath (PALS)) containing networks of fibroblastic reticular cells (FRC) surrounding a central arteriole, together with B cell follicles containing a central network of follicular dendritic cells (FDC).
- Marginal zones (MZ) surrounding the white pulp contain marginal reticular cells (MRC), particularly at the edges of the B cell follicles.
- Blood and leukocytes entering the spleen pass through branches of the central arteriole, which end in the marginal sinuses and red pulp.
- In the cords of the red pulp, a dense network of reticular fibroblasts and fibres construct an open blood network, which is marked by its lack of a typical endothelial cell lining.
- Large numbers of macrophages phagocytose dying or damaged red blood cells in the red pulp (not shown).
- Immune cells enter the white pulp at regions where the T cell zones abut the MZ, known as the MZ bridging channels.
|An image of a section of mouse spleen generated using multicolour immunofluoresence microscopy illustrates the organization of the white pulp, red pulp, and MZ (centre panel).
- The distribution of cells:
- T cells - (white) CD3+
- B cells - (blue) B220+
- macrophages - (cyan) CD169+ MZ
- dendritic cells - (green) (DCs) CD11c+
- stromal cells - (red) ER-TR7+
- The distinct organization of stromal cells in different regions of the spleen is shown by single-colour immunofluoresence staining (right panel).
- Networks of stromal cells and reticular fibres form in the white pulp, including the fibroblastic reticular cells (FRCs) in T cell zones, follicular dendritic cells (FDCs) in B cell follicles (ER-TR7−) and marginal reticular cells (MRCs) in the MZ.
- A dense network of stromal cells and reticular fibres is present in the red pulp.
Scale bars represent 130 μM.
<pubmed>19644499</pubmed>| PMC2785037 | Nat Rev Immunol.
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