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Sonic Hedgehog signalling increases the number of proliferating cells

Figure legend: SHH signaling increases the number of proliferating cells. (A–C,E–G) Images of cortical cell cultures after 2 (A–C) or 5 div (E–G) immunolabeled with antibodies against Ki67 (red) and MAP-2 (green). Cell nuclei are stained with DAPI (blue). (D,H) Quantification of Ki67-expressing cells. **p < 0.01, ***p < 0.001, One-Way ANOVA followed by Dunnet post-hoc test (Number of cells analyzed, 2 div—Control: 1863; Cyc: 2170; SHH: 2986; 5 div—Control: 5342; Cyc: 3015; SHH: 6170; 3 independent experiments). Calibration bar: 50 μm. ^[1]

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