View source for File:Reprogramming MEF into ES-like cells 03.jpg

==Reprogramming of genetically unmodified mouse embryonic fibroblasts into embryonic stem-like cells==

Outline of the mouse embryonic fibroblasts (MEF) reprogramming protocol. 
* 2.5×10<sup>5</sup> MEF cells were plated in two 100 mm dishes
* were infected with Sox2, Klf4, c-Myc and Oct3/4 virus
* Cells in dish 1(blue arrow) were passage on day 8 after infection
* ES-like colonies form 4 days later
* Cells in dish 2 (red arrow) were AP staining positive on day 14 and the colonies were counted. 

[[:File:Reprogramming MEF into ES-like cells 01.jpg]]
(B) Significant morphology changes observed on day 4 and ES-like colonies form on day 9 after MEF cells were infected with Sox2, Klf4, c-Myc and Oct3/4 virus. (C) Morphology of ES-like colonies on day 6 after passage. (D) AP positive colonies on day 14 post infection. (E) Estimated reprogramming efficiency of genetically unmodified MEF into AP positive ES-like cells 16 days post infection. 

[[:File:Reprogramming MEF into ES-like cells 02.jpg]]
(F) ES-like cell lines were established using the nonselective approach. Representative colonies from passage 6 cells were stained with anti-Rex1, Sox2 and SSEA1 antibodies and images were acquired through a Leica confocal system.


Original file name: Cr200792f1a.jpg

===Reference===
<pubmed>17971807</pubmed>| [http://www.nature.com/cr/journal/v17/n11/full/cr200792a.html Cell Research]


Reprinted by permission from Macmillan Publishers Ltd

Licensee: Mark A Hill  License Date: May 24, 2011 
License Number: 2675351286106  Publication: Cell Research 
Title: Direct generation of ES-like cells from unmodified mouse embryonic fibroblasts by Oct4/Sox2/Myc/Klf4
Type Of Use: post on the internet 

http://s100.copyright.com/CustomerAdmin/PLF.jsp?lID=2011051_1306242206106

[[Category:Stem Cell]] [[Category:Timeline]]