File:Mouse tooth movie 02.mp4: Difference between revisions
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== | ==Mouse Developing Tooth== | ||
Time-lapse imaging of a developing tooth germ derived from a transgenic mouse embryo (E 12.5–13.5). Co-expressing Fucci-G0/G1 probe and H2BEGFP. The tooth germ was cultured for 5 days ex vivo, and the images were taken at 30-min intervals. The lingual side is on the left. | |||
Time-lapse imaging of a developing tooth germ derived from a transgenic mouse embryo | |||
To visualize these tooth morphogenetic dynamics sequentially, we established an ex vivo time-lapse imaging system using transgenic mouse embryos co-expressing the Fucci-G0/G1 probe (Cdt1-mKO) and enhanced green fluorescent protein-labeled human histone 2B (H2B-EGFP; S1 and S2 Videos). | To visualize these tooth morphogenetic dynamics sequentially, we established an ex vivo time-lapse imaging system using transgenic mouse embryos co-expressing the Fucci-G0/G1 probe (Cdt1-mKO) and enhanced green fluorescent protein-labeled human histone 2B (H2B-EGFP; S1 and S2 Videos). | ||
[[Tooth Development]] | |||
===Reference=== | ===Reference=== | ||
Morita R, Kihira M, Nakatsu Y, Nomoto Y, Ogawa M, Ohashi K, et al. (2016) Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations. PLoS ONE 11(9): e0161336. https://doi.org/10.1371/journal.pone.0161336 | Morita R, Kihira M, Nakatsu Y, Nomoto Y, Ogawa M, Ohashi K, et al. (2016) Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations. PLoS ONE 11(9): e0161336. https://doi.org/10.1371/journal.pone.0161336 | ||
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0161336 | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0161336 | ||
S2 Video. | S2 Video (MP4). https://doi.org/10.1371/journal.pone.0161336.s010 | ||
{{Footer}} | {{Footer}} | ||
[[Category:MP4]][[Category:Tooth]][[Category:Mouse]][[Category:Mouse E12.5]] | [[Category:MP4]][[Category:Tooth]][[Category:Mouse]][[Category:Mouse E12.5]] |
Revision as of 14:35, 1 August 2017
Mouse Developing Tooth
Time-lapse imaging of a developing tooth germ derived from a transgenic mouse embryo (E 12.5–13.5). Co-expressing Fucci-G0/G1 probe and H2BEGFP. The tooth germ was cultured for 5 days ex vivo, and the images were taken at 30-min intervals. The lingual side is on the left.
To visualize these tooth morphogenetic dynamics sequentially, we established an ex vivo time-lapse imaging system using transgenic mouse embryos co-expressing the Fucci-G0/G1 probe (Cdt1-mKO) and enhanced green fluorescent protein-labeled human histone 2B (H2B-EGFP; S1 and S2 Videos).
Reference
Morita R, Kihira M, Nakatsu Y, Nomoto Y, Ogawa M, Ohashi K, et al. (2016) Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations. PLoS ONE 11(9): e0161336. https://doi.org/10.1371/journal.pone.0161336
Copyright
© 2016 Morita et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0161336 S2 Video (MP4). https://doi.org/10.1371/journal.pone.0161336.s010
Cite this page: Hill, M.A. (2024, April 24) Embryology Mouse tooth movie 02.mp4. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Mouse_tooth_movie_02.mp4
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G
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Date/Time | Dimensions | User | Comment | |
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current | 14:32, 1 August 2017 | (17.91 MB) | Z8600021 (talk | contribs) | S2 Video. Ex vivo live imaging of a developing tooth germ. embryonic days (E) 12.5–13.5 mice. Time-lapse imaging of a developing tooth germ derived from a transgenic mouse embryo co-expressing Fucci-G0/G1 probe and H2BEGFP. The tooth germ was cultu... |
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