File:Mouse pax7 trunk neural crest 02.jpg: Difference between revisions

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Mouse Trunk Neural Crest Pax7

At E11.5 in Pax7-cre/ROSA reporter mice, Pax7 descendants (YFP+ or LacZ+, respectively) are detected in the neural tube (NT), including commissural axons (asterisk), dermomyotome (DM) and dorsal root ganglia (DRG).

Only a subset of the total DRG neurons (TUJ1+, red) arise through the Pax7 lineage (YFP+).

Scale bar in B - 200 um

Reference

<pubmed>22848431</pubmed>| PMC2634972 | PLoS One.

Copyright

© 2012 Murdoch et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Figure 5. A-Fig-5.jpg doi:10.1371/journal.pone.0041089.g005

Mouse pax7 trunk neural crest 02.jpg

Figure 5 panel B cropped, resized and adjusted.

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	Date/Time	Thumbnail	Dimensions	User	Comment
current	10:33, 17 February 2013		881 × 691 (171 KB)	Z8600021 (talk \| contribs)	==Mouse Trunk Neural Crest Pax7== A–C) At E11.5 and D–H) E15.5 in Pax7-cre/ROSA reporter mice, Pax7 descendants (YFP+ or LacZ+, respectively) are detected in the neural tube (NT), including commissural axons (asterisk), dermomyotome (DM) and dorsal r

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Developmental Signals - Pax

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@@ Line 3: / Line 3: @@
 At E11.5 in Pax7-cre/ROSA reporter mice, Pax7 descendants (YFP+ or LacZ+, respectively) are detected in the neural tube (NT), including commissural axons (asterisk), dermomyotome (DM) and dorsal root ganglia (DRG).
-Only a subset of the total DRG neurons (TUJ1+, red) arise through the Pax7 lineage (YFP+, arrow).
+Only a subset of the total DRG neurons (TUJ1+, red) arise through the Pax7 lineage (YFP+).
-Scale bar in A -500 um
+Scale bar in B - 200 um
-===Pax7 lineage contributions to the Mammalian neural crest===
+{{Mouse neural crest pax7 links}}
-PLoS One. 2012;7(7):e41089. Epub 2012 Jul 27.
-Murdoch B, Delconte C, García-Castro MI.
-Source
-Biology Department, Eastern Connecticut State University, Willimantic, Connecticut, United States of America.
-Abstract
-BACKGROUND:
-Neural crest cells are vertebrate-specific multipotent cells that contribute to a variety of tissues including the peripheral nervous system, melanocytes, and craniofacial bones and cartilage. Abnormal development of the neural crest is associated with several human maladies including cleft/lip palate, aggressive cancers such as melanoma and neuroblastoma, and rare syndromes, like Waardenburg syndrome, a complex disorder involving hearing loss and pigment defects. We previously identified the transcription factor Pax7 as an early marker, and required component for neural crest development in chick embryos. In mammals, Pax7 is also thought to play a role in neural crest development, yet the precise contribution of Pax7 progenitors to the neural crest lineage has not been determined.
-METHODOLOGY/PRINCIPAL FINDINGS:
-Here we use Cre/loxP technology in double transgenic mice to fate map the Pax7 lineage in neural crest derivates. We find that Pax7 descendants contribute to multiple tissues including the cranial, cardiac and trunk neural crest, which in the cranial cartilage form a distinct regional pattern. The Pax7 lineage, like the Pax3 lineage, is additionally detected in some non-neural crest tissues, including a subset of the epithelial cells in specific organs.
-CONCLUSIONS/SIGNIFICANCE:
-These results demonstrate a previously unappreciated widespread distribution of Pax7 descendants within and beyond the neural crest. They shed light regarding the regionally distinct phenotypes observed in Pax3 and Pax7 mutants, and provide a unique perspective into the potential roles of Pax7 during disease and development.
-PMID 22848431
 Figure 5. A-Fig-5.jpg doi:10.1371/journal.pone.0041089.g005
@@ Line 31: / Line 17: @@
 Figure 5 panel B cropped, resized and adjusted.
+[[Category:Fluorescence]] [[Category:Mouse E11.5]]