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Mouse Left-Right Axis

Gastrula stage - Initially perinodal crown cells symmetrically express Nodal, Wnt and their antagonist Cerberus like-2 (Cerl2).

1-somite stage Cerl2 expression (green) becomes asymmetric with reduced expression on the left side of the node in response to fluid flow. With reduced expression of its antagonist on the left, Nodal activity and expression (dark blue) increases on the left side and decreases on the right.
2-somite stage wild type embryos, Nodal induce Nodal expression in the L-LPM (left lateral plate mesoderm)
3-somite stage robust expression of Nodal along with Lefty1 and Lefty2 (light blue) is detected in the L-LPM and Lefty1 in the midline (light blue).
4-somite stage embryos Nodal expression is reduced but Lefty1 and Lefty2 are strongly expressed in the L-LPM. Lefty1 expression in the midline and Cerl2 around the node inhibits Nodal signaling in the R-LPM.

Mouse E8.0 - (Theiler Stage 12a First Somites unturned embryo with first appearance of somite pairs 1-4 somites. The allantois extends further into the exocoelom and the maxillary components of the 1st branchial arch become prominent. The preotic sulcus is visible in the 2-3 somite embryo. The cardiogenic plate begins to form and the foregut pocket is clearly visible. Embryonic age = 8 dpc (range 7.5-8.75 dpc) 1-7 somite pairs

Mouse Stages: E1 | E2.5 | E3.0 | E3.5 | E4.5 | E5.0 | E5.5 | E6.0 | E7.0 | E7.5 | E8.0 | E8.5 | E9.0 | E9.5 | E10 | E10.5 | E11 | E11.5 | E12 | E12.5 | E13 | E13.5 | E14 | E14.5 | E15 | E15.5 | E16 | E16.5 | E17 | E17.5 | E18 | E18.5 | E19 | E20 | Timeline | About timed pregnancy

Species Embryonic Comparison Timeline

Carnegie

Stage

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

Human

Days

1

2-3

4-5

5-6

20

22

24

28

30

33

36

40

42

44

48

52

54

55

58

Mouse

Days

1

2

3

E10

E11

E12

E13

E14

E15

E16

Rat

Days

1

3.5

4-5

5

6

7.5

8.5

9

10.5

11

11.5

12

12.5

13

13.5

14

14.5

15

15.5

16

16.5

17

17.5

Note these Carnegie stages are only approximate day timings for average of embryos. Links: Carnegie Stage Comparison

Table References
Human O'Rahilly R. (1979). Early human development and the chief sources of information on staged human embryos. Eur. J. Obstet. Gynecol. Reprod. Biol. , 9, 273-80. PMID: 400868 Otis EM and Brent R. Equivalent ages in mouse and human embryos. (1954) Anat Rec. 120(1):33-63. PMID 13207763 Mouse Theiler K. The House Mouse: Atlas of Mouse Development (1972, 1989) Springer-Verlag, NY. Online OTIS EM & BRENT R. (1954). Equivalent ages in mouse and human embryos. Anat. Rec. , 120, 33-63. PMID: 13207763 Rat Witschi E. Rat Development. In: Growth Including Reproduction and Morphological Development. (1962) Altman PL. and Dittmer DS. ed. Fed. Am. Soc. Exp. Biol., Washington DC, pp. 304-314. Pérez-Cano FJ, Franch À, Castellote C & Castell M. (2012). The suckling rat as a model for immunonutrition studies in early life. Clin. Dev. Immunol. , 2012, 537310. PMID: 22899949 DOI.

Reference

Copyright

© 2017 Sugrue, Zohn. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Fig 6. https://doi.org/10.1371/journal.pone.0171180.g006 Wildtype panel cropped from full figure.

Cite this page: Hill, M.A. (2024, April 18) Embryology Mouse left-right axis 02.jpg. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/File:Mouse_left-right_axis_02.jpg

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Developmental Signals - Nodal

@@ Line 1: / Line 1: @@
-==Model for Generation of Left Isomerism in Ccdc40 versus Shh pathway mutants==
+==Mouse Left-Right Axis==
-In 1-somite stage wild type embryos, Cerl2 expression (green) becomes asymmetric with reduced expression on the left side of the node in response to fluid flow. With reduced expression of its antagonist on the left, Nodal activity and expression (dark blue) increases on the left side and decreases on the right. In 2-somite stage wild type embryos, Nodal induce Nodal expression in the L-LPM and by the 3-somite stage robust expression of Nodal along with Lefty1 and Lefty2 (light blue) is detected in the L-LPM and Lefty1 in the midline (light blue). In 4-somite stage embryos Nodal expression is reduced but Lefty1 and Lefty2 are strongly expressed in the L-LPM. Lefty1 expression in the midline and Cerl2 around the node inhibits Nodal signaling in the R-LPM. With lack of nodal flow in Ccdc40lnks and other cilia mutants with intact Shh signaling, Cerl2 and Nodal expression remains symmetric. Continued Cerl2 mediated antagonism of Nodal results in reduced Nodal signaling around the node and Nodal and Lefty1/2 expression in the LPM is delayed till the 3- and 4-somite stages, respectively. In a proportion of embryos where symmetry is not broken, Nodal signaling is reduced and bilateral. This is in contrast to IFT mutants with defects in Shh signaling. In these embryos, Nodal signaling is robustly induced bilaterally compounded by failure to activate the midline barrier.
+Gastrula stage - Initially perinodal crown cells symmetrically express Nodal, Wnt and their antagonist Cerberus like-2 (Cerl2).
+* 1-somite stage Cerl2 expression (green) becomes asymmetric with reduced expression on the left side of the node in response to fluid flow. With reduced expression of its antagonist on the left, Nodal activity and expression (dark blue) increases on the left side and decreases on the right.
+* 2-somite stage wild type embryos, Nodal induce Nodal expression in the L-LPM (left lateral plate mesoderm)
+* 3-somite stage robust expression of Nodal along with Lefty1 and Lefty2 (light blue) is detected in the L-LPM and Lefty1 in the midline (light blue).
+* 4-somite stage embryos Nodal expression is reduced but Lefty1 and Lefty2 are strongly expressed in the L-LPM. Lefty1 expression in the midline and Cerl2 around the node inhibits Nodal signaling in the R-LPM.
@@ Line 18: / Line 23: @@
 {{Footer}}
-[[Category:Mouse]][[Category:Mouse E8]][[Category:Developmental Mechanism]][[Category:Cartoon]]
+[[Category:Mouse]][[Category:Mouse E8.0]][[Category:Developmental Mechanism]][[Category:Cartoon]]