File:Mouse embryo vascular.png
Surface renderings of embryonic vascular structures
(A) Reconstructed FDR-deconvolution OPT data of the 19 somite embryo is shown as a surface rendered object.
(B) The surface rendered object can be zoomed in to any magnification, as in this magnified image of the vasculature in the mouse head.
(C) The surface rendering can also be rotated so that it can be viewed from any angle. Viewing the rendering from the left side reveals structures in the heart (arrow) that are obscured by the tail in (A).
(D) The 3D data can also be segmented as described in Materials and Methods. The DA, heart and ICAs are labelled yellow, the UV dark pink, and the unsegmented vasculature blue.
(E) Segmentation of the data allows selective display of labelled structures. Exclusion of the unsegmented data provides better analysis of the ICAs and the pharyngeal arch arteries.
All scale bars represent 100 microns.
- ACV - anterior cardinal vein
- CCV - common cardinal vein
- DA - dorsal aorta
- DLAV - dorsal longitudinal anastomotical vessel
- ISA - intersomitic artery
- ISV - intersomitic vein
- OA - omphalomesenteric artery
- OV - omphalomesenteric vein
- PCV - posterior cardinal vein
- PNVP - perineual vascular plexus
- UA - umbilical artery
- UV - umbilical vein
Original File name: Figure 2. Journal.pone.0002853.g002.png
<pubmed>18682734</pubmed>| PLoS One
- "Key vasculogenic (de-novo vessel forming) and angiogenic (vessel remodelling) events occur in the mouse embryo between embryonic days (E) 8.0 and 10.0 of gestation, during which time the vasculature develops from a simple circulatory loop into a complex, fine structured, three-dimensional organ. Interpretation of vascular phenotypes exhibited by signalling pathway mutants has historically been hindered by an inability to comprehensively image the normal sequence of events that shape the basic architecture of the early mouse vascular system. We have employed Optical Projection Tomography (OPT) using frequency distance relationship (FDR)-based deconvolution to image embryos immunostained with the endothelial specific marker PECAM-1 to create a high resolution, three-dimensional atlas of mouse vascular development between E8.0 and E10.0 (5 to 30 somites). Analysis of the atlas has provided significant new information regarding normal development of intersomitic vessels, the perineural vascular plexus, the cephalic plexus and vessels connecting the embryonic and extraembryonic circulation. We describe examples of vascular remodelling that provide new insight into the mechanisms of sprouting angiogenesis, vascular guidance cues and artery/vein identity that directly relate to phenotypes observed in mouse mutants affecting vascular development between E8.0 and E10.0. This atlas is freely available at http://www.mouseimaging.ca/research/mouse_atlas.html and will serve as a platform to provide insight into normal and abnormal vascular development."
Copyright: © 2008 Walls et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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|current||10:07, 15 August 2009||600 × 576 (518 KB)||S8600021||Figure 2. Surface renderings of embryonic vascular structures. (A) Reconstructed FDR-deconvolution OPT data of the 19 somite embryo is shown as a surface rendered object. (B) The surface rendered object can be zoomed in to any magnification, as in this|
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