File:Mouse Lmx1b gene expression.jpg

Mouse Lmx1b Gene Expression

Lmx1b LIM homeobox transcription factor 1 beta (Lmx1b) expressed in the dorsal mesenchyme (b).

Lmx1b: limb dorsalization | Gene | PMID 22417325

Limb Links: Mouse limb skeleton cartoon | Mouse limb tissue development | Limb Development | Mouse Development

Gene Expression Patterns: E9.5 | E10.5 | E11.5 | E12.5 | E13.5 | Lmx1b | Forelimb | Tbx5 | Fbxo41 | Hoxc5 | Hoxb5 | Prdm1 | Hoxb6 | Etv2 | Egr1 | Figf | Sp6 | Cited1 | Prox1 | SmarcD2 | Echdc1 | Sim2 | Hindlimb | Tbx4 | Ptx1 | Hoxc10 | Hoxc9 | Dmrt2

Reference

Taher L, Collette NM, Murugesh D, Maxwell E, Ovcharenko I & Loots GG. (2011). Global gene expression analysis of murine limb development. PLoS ONE , 6, e28358. PMID: 22174793 DOI.

Copyright

This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

Figure 3. doi:10.1371/journal.pone.0028358.g003

Detection of genes regulated by Lmx1b during limb dorsalization

Dev Growth Differ. 2012 May;54(4):451-62. doi: 10.1111/j.1440-169X.2012.01331.x. Epub 2012 Mar 15.

Feenstra JM, Kanaya K, Pira CU, Hoffman SE, Eppey RJ, Oberg KC. Source Division of Human Anatomy, Department of Pathology and Human Anatomy, Loma Linda University School of Medicine, Loma Linda, CA 92350, USA.

Abstract

Lmx1b is a homeodomain transcription factor that regulates dorsal identity during limb development. Lmx1b knockout (KO) mice develop distal ventral-ventral limbs. Although induction of Lmx1b is linked to Wnt7a expression in the dorsal limb ectoderm, the downstream targets of Lmx1b that accomplish limb dorsalization are unknown. To identify genes targeted by Lmx1b, we compared gene arrays from Lmx1b KO and wild type mouse limbs during limb dorsalization, i.e., 11.5, 12.5, and 13.5 days post coitum. We identified 54 target genes that were differentially expressed in all three stages. Several skeletal targets, including Emx2, Matrilin1 and Matrilin4, demonstrated a loss of scapular expression in the Lmx1b KO mice, supporting a role for Lmx1b in scapula development. Furthermore, the relative abundance of extracellular matrix-related soft tissue targets regulated by Lmx1b, such as collagens and proteoglycans, suggests a mechanism that includes changes in the extracellular matrix composition to accomplish limb dorsalization. Our study provides the most comprehensive characterization of genes regulated by Lmx1b during limb development to-date and provides targets for further investigation. © 2012 The Authors. Development, Growth & Differentiation © 2012 Japanese Society of Developmental Biologists.

PMID 22417325