File:Model GATA6 hindlimb.jpg

Model for GATA6 action Patterning developing Hindlimb Buds

GATA6 is expressed in the anterior portion of the developing mouse hindlimb bud (displayed in top figures), and specifically represses polydactyly (PD) in the hindlimb by directly blocking anterior expression of Shh (most likely in a FOG-dependent fashion), and thus represses ectopic expression of both direct Shh transcriptional targets (Gli1 and Grem1) in anterior limb bud mesenchyme and indirect targets (FGF4/8) in the anterior AER. In hindlimb buds engineered to lack GATA6 (displayed in bottom figures), ectopic expression of Shh in the anterior mesenchyme induces expression of both Gli2/3 FL and Gli1 which activate expression of the BMP antagonist Grem1, which in turn derepresses expression of FGF4/8 in the anterior region of the apical ectodermal ridge. Thus, as a consequence of deletion of GATA6 in Prx1-Cre; GATA6fl/fl animals, the hindlimbs of these animals display preaxial polydactyly. Expressed genes are indicated by black font; knocked-out or repressed genes are indicated by grey font.

Reference

<pubmed>24415953</pubmed>| PLoS Genet.

Copyright

© 2014 Kozhemyakina et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Figure 7. doi:10.1371/journal.pgen.1004072.g007 Journal.pgen.1004072.g007.png