File:Human spermatid electron micrograph.jpg: Difference between revisions

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A × 20,000; B × 16,000.
A × 20,000; B × 16,000.


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Revision as of 10:57, 17 September 2014

Human Spermatid EM

Electron micrographs of human spermatids following post-embedding labelling with anti-SPANXa/d antibodies.

(A) In this cap-phase spermatid staining was not observed associated with the nuclear envelope (ne) underlying the developing acrosome (a). However, gold particles (5 nm) were present on membrane whorls lying within a nuclear vacuole (NV) that opened into the subacrosomal space.

(B) In this elongated spermatid gold particles were associated with both the nucleoplasmic and the cytoplasmic faces of the nuclear envelope (ne) posterior to the acrosome (a) and adjacent to the manchette microtubules (M, white arrows). Staining of the nuclear envelope was not observed underlying the developing acrosome (a, bars mark posterior aspect of acrosome) or in the region immediately posterior to the acrosomal bulbs where the nuclear envelope is closely apposed to the nucleus.

The inset shows a fold of the nuclear envelope at the posterior region of the spermatid nucleus which exhibited a concentration of gold particles indicative of SPANXa/d staining. M, manchette; N, nucleus.

A × 20,000; B × 16,000.


Human Spermatozoa EM: Image - cap-phase spermatid | Image - elongated spermatid | Image - spermatid | Image - spermatozoa | Image - normal nucleus | Image - nucleus | Image - abnormal nucleus | Spermatozoa Development | Category:Electron Micrograph


Spermatozoa Images: Spermatozoa BF | Spermatozoon BF | Spermatozoon EM | Spermatozoon EM | Historic drawing | Category:Spermatozoa | Spermatozoa Development | Testis Development

Reference

<pubmed>17012309</pubmed>| Mol Hum Reprod.


Copyright

© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org


Original file name: Figure 8. http://molehr.oxfordjournals.org/content/12/11/703/F8.expansion.html

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current16:11, 1 November 2010Thumbnail for version as of 16:11, 1 November 2010619 × 918 (206 KB)S8600021 (talk | contribs)==Human Spermatid EM== Electron micrographs of human spermatids following post-embedding labelling with anti-SPANXa/d antibodies. (A) In this cap-phase spermatid staining was not observed associated with the nuclear envelope (ne) underlying the develop

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