File:Figure 2 - defects of mitochondria in CTCF mutant hearts.PNG

From Embryology

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Description

Defective mitochondrial biogenesis upon CTCF loss. Western blot of Cox I, Cox IV (A), Grp75, Tfam and Tom20 (B) of control and mutant (Ctcffl/fl;Nkx2-5-Cre) hearts at E10.5 and E11.5. (C) Blue native gel of control and mutant hearts at E10.5 and E11.5 showing abundance and distribution of mitochondrial respiratory complexes (CI, CIV, CVm) and supercomplexes (CI+CIII). (D) Densitometry of blue native gel quantifying changes between E10.5 and E11.5 in complexes and supercomplexes, in control and mutant hearts. (E) Transmission Electron Microscopy at E10.5 and E11.5 in control and mutant cardiomyocytes. n, nucleus; sm, sarcomere. Arrowheads point to mitochondria. Scale bar, 1 μm top row, 0.5 μm bottom row.

Reference

Melisa Gomez-Velazquez, Claudio Badia-Careaga, Ana Victoria Lechuga-Vieco, Rocio Nieto-Arellano, Juan J Tena, Isabel Rollan, Alba Alvarez, Carlos Torroja, Eva F Caceres, Anna Roy, Niels Galjart, Paul Delgado-Olguin, Fatima Sanchez-Cabo, Jose Antonio Enriquez, Jose Luis Gomez-Skarmeta, Miguel Manzanares CTCF counter-regulates cardiomyocyte development and maturation programs in the embryonic heart. PLoS Genet.: 2017, 13(8);e1006985 PubMed 28846746

Copyright

© 2017 Gomez-Velazquez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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