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=Electron micrographs of granulosa cells (GC)=
A, B: The integrity of follicles was well preserved. Nucleus, oocyte and granulosa cells (GC) were indicated. C: Nucleus of granulosa cells (GC) was dissolved after treated with slow-rate freezing. Overall the proportion of alterations (change of microvilli, change of mitochondria cristae, change of GC and rupture of oolemma) was relatively less in follicles vitrified by SSV compared to follicles vitrified by OPS and frozen by '''slow-rate freezing'''. Therefore, SSV method is an appropriate and convenient method for cryopreservation of isolated follicles compared with the conventional slow-rate freezing <ref><pubmed>20459796</pubmed></ref> .
(Original figure legend :1477-7827-8-42-7)
(Original figure legend :1477-7827-8-42-7)
PMID 20459796
 
===References===
 
<references/>
 
=== Copyright===
 
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
 
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Latest revision as of 13:48, 18 October 2015

Electron micrographs of granulosa cells (GC)

A, B: The integrity of follicles was well preserved. Nucleus, oocyte and granulosa cells (GC) were indicated. C: Nucleus of granulosa cells (GC) was dissolved after treated with slow-rate freezing. Overall the proportion of alterations (change of microvilli, change of mitochondria cristae, change of GC and rupture of oolemma) was relatively less in follicles vitrified by SSV compared to follicles vitrified by OPS and frozen by slow-rate freezing. Therefore, SSV method is an appropriate and convenient method for cryopreservation of isolated follicles compared with the conventional slow-rate freezing [1] .


(Original figure legend :1477-7827-8-42-7)

References

  1. <pubmed>20459796</pubmed>

Copyright

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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