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'''Image source:''' Klimanskaya I, Chung Y, Becker S, Lu SJ, Lanza R. Human embryonic stem cell lines derived from single blastomeres. Nature. 2006 Aug 23
==Human Embryo Carnegie stage 3==
[http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16929302 PMID:16929302]


:The derivation of human embryonic stem (hES) cells currently requires the destruction of ex utero embryos. A previous study in mice indicates that it might be possible to generate embryonic stem (ES) cells using a single-cell biopsy similar to that used in preimplantation genetic diagnosis (PGD), which does not interfere with the embryo's developmental potential. By growing the single blastomere overnight, the resulting cells could be used for both genetic testing and stem cell derivation without affecting the clinical outcome of the procedure. Here we report a series of ten separate experiments demonstrating that hES cells can be derived from single blastomeres. In this proof-of-principle study, multiple biopsies were taken from each embryo using micromanipulation techniques and none of the biopsied embryos were allowed to develop in culture. Nineteen ES-cell-like outgrowths and two stable hES cell lines were obtained. The latter hES cell lines maintained undifferentiated proliferation for more than eight months, and showed normal karyotype and expression of markers of pluripotency, including Oct-4, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81, nanog and alkaline phosphatase. These cells retained the potential to form derivatives of all three embryonic germ layers both in vitro and in teratomas. The ability to create new stem cell lines and therapies without destroying embryos would address the ethical concerns of many, and allow the generation of matched tissue for children and siblings born from transferred PGD embryos.
Human Blastocyst "hatching" from zona pellucida, in early [[Embryonic Development]] designated as [[Carnegie stage 3]].
 
The '''zona pellucida''' is shown to the left of the image and the '''blastocyst''' is to the right of image.
 
'''Note:'''
* the small opening in the zona pellucida through which the blastocyst is hatching
* the flattened trophoblast cells forming the outer cell layer of the blastocyst
* the inner cell mass shown in the centre of the image and on the left-hand wall of the blastocyst
* the blastocoel forming a large fluid-filled space within the blastocyst
 
 
:'''Links:''' [[:File:CSt3.jpg|unlabeled image]] | [[:File:Human carnegie stage 3 label.jpg|labeled image]] | [[Carnegie stage 3]] | [[Blastocyst Development]] | [[Week 1]]
 
 
===Reference===
<pubmed>16929302</pubmed>| [http://www.nature.com/nature/journal/v444/n7118/full/nature05142.html Nature]
 
====Copyright====


Adapted by permission from Macmillan Publishers Ltd (copyright 2006)
Adapted by permission from Macmillan Publishers Ltd (copyright 2006)
Original image name: Figure 1 part b, Development of a blastomere-biopsied embryo into a hatching blastocyst. http://www.nature.com/nature/journal/v444/n7118/fig_tab/nature05142_F1.html (image extracted from full figure and adjusted in size and contrast)
[[Category:Human Embryo]] [[Category:Carnegie Stage]] [[Category:Carnegie Stage 3]] [[Category:Week 1]] [[Category:Blastocyst]]

Latest revision as of 14:38, 29 November 2012

Human Embryo Carnegie stage 3

Human Blastocyst "hatching" from zona pellucida, in early Embryonic Development designated as Carnegie stage 3.

The zona pellucida is shown to the left of the image and the blastocyst is to the right of image.

Note:

  • the small opening in the zona pellucida through which the blastocyst is hatching
  • the flattened trophoblast cells forming the outer cell layer of the blastocyst
  • the inner cell mass shown in the centre of the image and on the left-hand wall of the blastocyst
  • the blastocoel forming a large fluid-filled space within the blastocyst


Links: unlabeled image | labeled image | Carnegie stage 3 | Blastocyst Development | Week 1


Reference

<pubmed>16929302</pubmed>| Nature

Copyright

Adapted by permission from Macmillan Publishers Ltd (copyright 2006)


Original image name: Figure 1 part b, Development of a blastomere-biopsied embryo into a hatching blastocyst. http://www.nature.com/nature/journal/v444/n7118/fig_tab/nature05142_F1.html (image extracted from full figure and adjusted in size and contrast)

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current14:07, 9 May 2011Thumbnail for version as of 14:07, 9 May 2011500 × 377 (20 KB)S8600021 (talk | contribs)
13:55, 9 May 2011Thumbnail for version as of 13:55, 9 May 2011500 × 377 (12 KB)S8600021 (talk | contribs)
13:54, 9 May 2011Thumbnail for version as of 13:54, 9 May 2011500 × 377 (12 KB)S8600021 (talk | contribs)
13:32, 2 August 2009Thumbnail for version as of 13:32, 2 August 2009285 × 219 (11 KB)S8600021 (talk | contribs)Image source: Klimanskaya I, Chung Y, Becker S, Lu SJ, Lanza R. Human embryonic stem cell lines derived from single blastomeres. Nature. 2006 Aug 23 [http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16929302

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