File:Bovine blastocyst 01.jpg

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Bovine Blastocyst

G: Cell death in the inner cell mass of bovine IVF blastocysts examined at day 7: In expanding and hatching blastocysts, DAPI staining reveals highly condensed chromatin structures that are irregularly shaped and variable in size referring to different modes and stages of cell death (arrows).

Image is a maximum intensity z-projection of a 20 µm image stack

The embryos were fixed and mounted on coverslips in such a way that the three-dimensional structure was maintained. DNA staining with DAPI is shown in white, f-actin filaments (phalloidin-TRITC) in orange.

Scale bars represent 100 µm (overviews) or 10 µm (details).


Links: Bovine Development


Figure 2. CLSM analysis (Panel G cropped from full image)

Reference

<pubmed>21811561</pubmed>| PLoS One.

Copyright: © 2011 Leidenfrost et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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current14:23, 4 November 2011Thumbnail for version as of 14:23, 4 November 2011688 × 1,000 (99 KB)S8600021 (talk | contribs)resized image
14:22, 4 November 2011Thumbnail for version as of 14:22, 4 November 2011516 × 750 (65 KB)S8600021 (talk | contribs)==Bovine Blastocyst== G: Cell death in the inner cell mass of bovine IVF blastocysts examined at day 7: In expanding and hatching blastocysts, DAPI staining reveals highly condensed chromatin structures that are irregularly shaped and variable in size re

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