Endocrine - Pancreas Development: Difference between revisions
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==Introduction== | ==Introduction== | ||
[[File:Human- pancreatic adult islet.jpg|thumb|350px|Human Pancreatic Islets (Islets of Langerhans) | [[File:Human- pancreatic adult islet.jpg|thumb|350px|Human Pancreatic Islets (Islets of Langerhans){{#pmid:18958289|PMID18958289}}]] | ||
The pancreas is a two-headed organ, not only in origin but also in function. In origin, the pancreas develops from two separate primordia. In function, the organ has both endocrine function in relation to regulating blood glucose (and also other hormone secretions) and gastrointestinal function as an exocrine (digestive) organ, see [[Gastrointestinal Tract - Pancreas Development]]. | The pancreas is a two-headed organ, not only in origin but also in function. In origin, the pancreas develops from two separate primordia. In function, the organ has both endocrine function in relation to regulating blood glucose (and also other hormone secretions) and gastrointestinal function as an exocrine (digestive) organ, see [[Gastrointestinal Tract - Pancreas Development]]. | ||
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::[[Embryology_History|Historic Embryology]]: [[Book - Manual of Human Embryology 17-8|1912 Pancreas Development]] | [[Paper - Some Observations on the Development of the Ventral Pancreas in Man|1930 Ventral Pancreas]] | ::[[Embryology_History|Historic Embryology]]: [[Book - Manual of Human Embryology 17-8|1912 Pancreas Development]] | [[Paper - Some Observations on the Development of the Ventral Pancreas in Man|1930 Ventral Pancreas]] | ||
== Some Recent Findings == | == Some Recent Findings == | ||
[[File:Molecular_endocrine_pancreas_cells_01.jpg|thumb|300px|alt=Molecular Development of Endocrine Pancreas Cells|Molecular Development of Endocrine Pancreas Cells | [[File:Molecular_endocrine_pancreas_cells_01.jpg|thumb|300px|alt=Molecular Development of Endocrine Pancreas Cells|Molecular Development of Endocrine Pancreas Cells{{#pmid:23940571|PMID23940571}}[[#Molecular|Molecular Pancreas]]]] | ||
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* '''Gastrin: a distinct fate of neurogenin3 positive progenitor cells in the embryonic pancreas''' | * '''Gastrin: a distinct fate of neurogenin3 positive progenitor cells in the embryonic pancreas'''{{#pmid:23940571|PMID23940571}} "Neurogenin3(+) (Ngn3(+)) progenitor cells in the developing pancreas give rise to five endocrine cell types secreting insulin, glucagon, somatostatin, pancreatic polypeptide and ghrelin. Gastrin is a hormone produced primarily by G-cells in the stomach, where it functions to stimulate acid secretion by gastric parietal cells. Gastrin is expressed in the embryonic pancreas and is common in islet cell tumors, but the lineage and regulators of pancreatic gastrin(+) cells are not known. We report that gastrin is abundantly expressed in the embryonic pancreas and disappears soon after birth." | ||
* '''Chemical screen identifies FDA-approved drugs and target pathways that induce precocious pancreatic endocrine differentiation''' | |||
* '''Pancreatic mesenchyme regulates epithelial organogenesis throughout development''' | * '''Chemical screen identifies FDA-approved drugs and target pathways that induce precocious pancreatic endocrine differentiation'''{{#pmid:22084084|PMID22084084}} "Pancreatic β-cells are an essential source of insulin and their destruction because of autoimmunity causes type I diabetes. We conducted a chemical screen to identify compounds that would induce the differentiation of insulin-producing β-cells in vivo. To do this screen, we brought together the use of transgenic zebrafish as a model of β-cell differentiation, a unique multiwell plate that allows easy visualization of lateral views of swimming larval fish and a library of clinical drugs. We identified six hits that can induce precocious differentiation of secondary islets in larval zebrafish. Three of these six hits were known drugs with a considerable background of published data on mechanism of action. Using pharmacological approaches, we have identified and characterized two unique pathways in β-cell differentiation in the zebrafish, including down-regulation of GTP production and retinoic acid biosynthesis." | ||
* '''Relative roles of the different Pax6 domains for pancreatic alpha cell development.''' | |||
* '''Pancreatic mesenchyme regulates epithelial organogenesis throughout development'''{{#pmid:21909240|PMID21909240}} "The developing pancreatic epithelium gives rise to all endocrine and exocrine cells of the mature organ. During organogenesis, the epithelial cells receive essential signals from the overlying mesenchyme. ...Our results demonstrate that mesenchymal cells regulate pancreatic growth and branching at both early and late developmental stages by supporting proliferation of precursors and differentiated cells, respectively." | |||
* '''Relative roles of the different Pax6 domains for pancreatic alpha cell development.'''{{#pmid:20377917|PMID20377917}} "The transcription factor Pax6 functions in the specification and maintenance of the differentiated cell lineages in the endocrine pancreas. It has two DNA binding domains, the paired domain and the homeodomain, in addition to a C-terminal transactivation domain. The phenotype of Pax6-/- knockout mice suggests non-redundant functions of the transcription factor in the development of glucagon-expressing alpha-cells as this cell type is absent in the mutants." | |||
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[[File:Human_fetal_pancreas_anatomy_cartoon.jpg|500px]] | [[File:Human_fetal_pancreas_anatomy_cartoon.jpg|500px]] | ||
Fetal topographical anatomy of the pancreatic head and duodenum with special reference to courses of the pancreaticoduodenal arteries. | Fetal topographical anatomy of the pancreatic head and duodenum with special reference to courses of the pancreaticoduodenal arteries.{{#pmid:20376893|PMID20376893}} | ||
A diagram showing joining processes between the dorsal and ventral primordia of the pancreas as well as the hypothetical rotation of the duodenum along a left-right axis. Viewed from the posterosuperior side of the body. A horizontal plane including most parts of the duodenum is shown to emphasize, in contrast to adults, the course of the second portion (D2) directing posteriorly rather than inferiorly. | A diagram showing joining processes between the dorsal and ventral primordia of the pancreas as well as the hypothetical rotation of the duodenum along a left-right axis. Viewed from the posterosuperior side of the body. A horizontal plane including most parts of the duodenum is shown to emphasize, in contrast to adults, the course of the second portion (D2) directing posteriorly rather than inferiorly. | ||
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==Developing Pancreatic Islets== | ==Developing Pancreatic Islets== | ||
{| | {| | ||
| '''Model of endocrine cell and vessel organization in human islets''' | | '''Model of endocrine cell and vessel organization in human islets'''{{#pmid:20185817|PMID20185817}} | ||
[[File:Model of human pancreatic islet.jpg|400px]] | [[File:Model of human pancreatic islet.jpg|400px]] | ||
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==Adult Pancreatic Islets== | ==Adult Pancreatic Islets== | ||
[[File:3D_Human_pancreatic_islet.jpg|thumb|Human pancreatic islet in 3D | [[File:3D_Human_pancreatic_islet.jpg|thumb|Human pancreatic islet in 3D{{#pmid:20185817|PMID20185817}}]] | ||
[[File:Pancreas islet - structure human and rat.jpg|thumb|Pancreas islet structure human and rat]] | [[File:Pancreas islet - structure human and rat.jpg|thumb|Pancreas islet structure human and rat]] | ||
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[[File:Rat-_pancreatic_islet_development.jpg|Rat- pancreatic islet development|500px]] | [[File:Rat-_pancreatic_islet_development.jpg|Rat- pancreatic islet development|500px]] | ||
Rat - pancreatic islet development | Rat - pancreatic islet development{{#pmid:19534767|PMID19534767}} | ||
==Islet size for Different Species== | ==Islet size for Different Species== | ||
The following species comparison table has been slightly modified from Table 1 data | The following species comparison table has been slightly modified from Table 1 data from a review paper.{{#pmid:20606719|PMID20606719}} | ||
* Islet size is described as an effective diameter of a circle, which depicts the same area as a measured islet area. | * Islet size is described as an effective diameter of a circle, which depicts the same area as a measured islet area. | ||
* β-cell ratio is the area ratio of β-cells in an islet. | * β-cell ratio is the area ratio of β-cells in an islet. | ||
* Both data sets are expressed as the mean value with its standard deviation. | * Both data sets are expressed as the mean value with its standard deviation. | ||
{{Pancreas islet species comparison table}} | |||
==Hormones== | ==Hormones== |
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Introduction
The pancreas is a two-headed organ, not only in origin but also in function. In origin, the pancreas develops from two separate primordia. In function, the organ has both endocrine function in relation to regulating blood glucose (and also other hormone secretions) and gastrointestinal function as an exocrine (digestive) organ, see Gastrointestinal Tract - Pancreas Development.
In recent years there has been much research due to the increasing incidence of diabetes in humans and the potential for stem cell therapeutics. Much is now known about the epithelial/mesenchymal and molecular regulation of pancres development.
At the foregut/midgut junction the septum transversum generates 2 pancreatic buds (dorsal and ventral endoderm) which will fuse to form the pancreas. The dorsal bud arises first and generates most of the pancreas. The ventral bud arises beside the bile duct and forms only part of the head and uncinate process of the pancreas.
In the fetal period islet cell clusters (icc) differentiate from pancratic bud endoderm. These cell clusters form acini and ducts (exocrine). On the edge of these cell clusters pancreatic islets (endocrine) also form. Pancreatic hormonal function is to secrete insulin and glucagon which together regulate blood glucose levels and also somaostatin.
The pancreas exocrine function begins after birth, while the endocrine function (hormone release) can be measured from 10 to 15 weeks onward. At this stage, it is not clear what the exact roles of these hormones are in regulating fetal growth.
- Functions - exocrine (amylase, alpha-fetoprotein), 99% by volume; endocrine (pancreatic islets) 1% by volume
- Exocrine function - begins after birth
- Endocrine function - from 10 to 15 weeks onward hormone release
- exact roles of hormones in regulating fetal growth?
- Links: Endocrine Pancreas | Exocrine Pancreas
See also: Lecture - Gastrointestinal Development | Maternal Diabetes
Some Recent Findings
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More recent papers |
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This table allows an automated computer search of the external PubMed database using the listed "Search term" text link.
More? References | Discussion Page | Journal Searches | 2019 References | 2020 References Search term: Pancreas Embryology <pubmed limit=5>Pancreas Embryology</pubmed> |
Pancreas Development
- Pancreatic buds - duodenal level endoderm, splanchnic mesoderm forms dorsal and ventral mesentery, dorsal bud (larger, first), ventral bud (smaller, later)
- Pancreas Endoderm - pancreas may be opposite of liver
- Heart cells promote/notochord prevents liver formation
- Notochord may promote pancreas formation
- Heart may block pancreas formation
- Duodenum growth/rotation - brings ventral and dorsal buds together, fusion of buds
- Pancreatic duct - ventral bud duct and distal part of dorsal bud, exocrine function
- Islet cells - cords of endodermal cells form ducts, from which cells bud off to form islets
PMID 18508724
Human Pancreas Timeline
- Week 7 to 20 - pancreatic hormones secretion increases, small amount maternal insulin
- Week 10 - glucagon (alpha) differentiate first, somatostatin (delta), insulin (beta) cells differentiate, insulin secretion begins
- Week 15 - glucagon detectable in fetal plasma
Mouse pancreas duct development cartoon
Pig embryo (14 mm CRL) (ventral and dorsal)
Fetal Pancreas
Fetal topographical anatomy of the pancreatic head and duodenum with special reference to courses of the pancreaticoduodenal arteries.[6]
A diagram showing joining processes between the dorsal and ventral primordia of the pancreas as well as the hypothetical rotation of the duodenum along a left-right axis. Viewed from the posterosuperior side of the body. A horizontal plane including most parts of the duodenum is shown to emphasize, in contrast to adults, the course of the second portion (D2) directing posteriorly rather than inferiorly.
Developing Pancreatic Islets
Model of endocrine cell and vessel organization in human islets[7] | A α-Cells (green) and β-cells (red) are organized into a thick folded plate lined at both sides with vessels (blue).
|
Adult Pancreatic Islets
The adult pancreatic islets (Islets of Langerhans) contain four distinct endocrine cell types.
Alpha Cells
- glucagon, mobilizes lipid
Beta Cells
- insulin, increase glucose uptake
- stimulate fetal growth, continue to proliferate to postnatal, in infancy most abundant
Molecular - Nkx6.1 - NK2 Homeobox 6.1
- homeobox (Hox) containing transcription factor contain a 60-amino acid evolutionarily conserved DNA-binding homeodomain.
- required for beta cells development and is completely conserved between rat, mouse, and human.
- Links: Maternal Diabetes | Nkx6.1 OMIM 602563
Delta Cells
- somatostatin, inhibits glucagon, insulin secretion
F-cells
- pancreatic polypeptide
Rat - pancreatic islet development[8]
Islet size for Different Species
The following species comparison table has been slightly modified from Table 1 data from a review paper.[9]
- Islet size is described as an effective diameter of a circle, which depicts the same area as a measured islet area.
- β-cell ratio is the area ratio of β-cells in an islet.
- Both data sets are expressed as the mean value with its standard deviation.
Pancreas Islet Species Comparison | |||
---|---|---|---|
Species | Age | Islet size (μm) | β-cell ratio |
Human | 39 years (adult) | 50 ± 29 | 0.64 ± 0.21 |
Monkey | 1 year | 67 ± 38* | 0.79 ± 0.14* |
Pig | 6 month | 49 ± 15a | 0.89 ± 0.11* |
Rabbit | 6 month | 64 ± 28* | 0.79 ± 0.17* |
Bird | 40 day | 24 ± 6* | 0.46 ± 0.24* |
Wild-type mouse | 6 month | 116 ± 80* | 0.85 ± 0.14* |
Pregnant mouse | 3 month | 112 ± 94* | 0.84 ± 0.22* |
ob/ob mouse | 15 week | 86 ± 76* | 0.92 ± 0.11* |
db/db mouse | 15 week | 47 ± 24b | 0.53 ± 0.24c |
*p < 0.0001 ap = 0.65 bp = 0.42 cp = 0.0004 compared with human.
| |||
Reference: [9] |
Hormones
Insulin
- Source - synthesized by the beta cells of the islets of Langerhans.
- Protein
- 2 dissimilar polypeptide chains, A and B, which are linked by 2 disulphide bonds.
- both chains are derived from a 1-chain precursor, proinsulin.
- proinsulin - converted to insulin by the enzymatic removal of a segment that connects the amino end of the A chain to the carboxyl end of the B chain.
- Links: OMIM
Glucagon
- Source - synthesized by the alpha cells of the islets of Langerhans.
- Protein
- 29-amino acid hormone
- human, rabbit, rat, pig, and cow proteins are identical.
- member of a multigene family that includes - secretin, vasoactive intestinal peptide, gastric inhibitory peptide, glicentin, and others.
- Function
- counteracts the glucose-lowering action of insulin
- stimulates glycogenolysis and gluconeogenesis.
- Links: OMIM
Molecular
Mouse Pancreas Cell Lineage
In this study[10] mouse cell types were collected at different ages E11 and E15 pancreatic progenitors, E15 acinar cells, E15 endocrine progenitors (EP), E15, E17, P1, P15, 8–12 week beta cells, P1 and 8–12 week alpha cells, and adult duct cells. The following markers were used in determining the lineages, not both endocrine and exocrine cells derive from a common precursor.
|
Identification of pancreas cell lineages[10] |
Developmental Factors | |
---|---|
|
Molecular Development of Endocrine Pancreas Cells[2] |
- Links: Molecular Development
Pancreas Histology
- Pancreas Histology Links: overview (label) | exocrine (label) | endocrine (label) | blood vessels (label) | insulin (label) | overview | exocrine | endocrine | blood vessels | insulin | Islet labeled for insulin and Glucagon | Insulin (Fl) | Glucagon (Fl) | GIT Histology
Diabetes
Diabetes is a condition where pancreatic insulin is no longer produced in sufficient required amounts (or at all) meaning that glucose cannot be converted into energy, resulting in health issues related to blood sugar levels. There are two main types:
- Type 1 diabetes - (10% of all cases) most common chronic childhood condition. An auto-immune condition, where the immune system is activated to destroy the beta cells in the pancreas which produce insulin. Type 1 diabetes is not linked to modifiable lifestyle factors.
- Type 2 diabetes - (85–90% of all cases) most common in adults. A progressive condition in which the body becomes resistant to the normal effects of insulin and/or gradually loses the capacity to produce enough insulin in the pancreas. Type 2 diabetes is associated with modifiable lifestyle risk factors and has strong genetic and family related risk factors.
Secondary Health Issues:
- Diabetic retinopathy - is a leading cause of preventable blindness.
- Diabetic ketoacidosis (DKA) occurs among children and young people with type 1 diabetes and is 1.4 times higher in females.
- Links: Maternal Diabetes | External Links
Abnormalities
Listed below are a number of pancreatic developmental abnormalities, see also the 2003 article "Lifetime consequences of abnormal fetal pancreatic development"[11].
Accessory Pancreatic Tissue - pancreatic tissue located in associated gastrointestinal tract tissues/organs such as the wall of the stomach, duodenum, jejunum or Meckel's diverticulum.
Annular Pancreas - (1 in 7,000 people) pancreas forms as a "ring" of tissue surrounding the duodenum which is subsequently narrowed.
Diabetes Mellitus - Maternal diabetes (and hyperglycaemia) have been shown to lead to increased fetal islet hyperplasia of the insulin producing beta cells and insulin secretion.
Intrauterine growth restriction - can lead to a delayed development of the insulin producing beta cells and low insulin secretion.
Tumours - Serous Cystadenoma (endocrine tumour), Somatostatinoma (tumour of delta cell origin), intraductal papillary-mucinous neoplasm
Diabetic ketoacidosis (DKA) occurs among children and young people with type 1 diabetes and is 1.4 times higher in females.
References
- ↑ Scharfmann R, Xiao X, Heimberg H, Mallet J & Ravassard P. (2008). Beta cells within single human islets originate from multiple progenitors. PLoS ONE , 3, e3559. PMID: 18958289 DOI.
- ↑ 2.0 2.1 2.2 Suissa Y, Magenheim J, Stolovich-Rain M, Hija A, Collombat P, Mansouri A, Sussel L, Sosa-Pineda B, McCracken K, Wells JM, Heller RS, Dor Y & Glaser B. (2013). Gastrin: a distinct fate of neurogenin3 positive progenitor cells in the embryonic pancreas. PLoS ONE , 8, e70397. PMID: 23940571 DOI. Cite error: Invalid
<ref>
tag; name 'PMID23940571' defined multiple times with different content - ↑ Rovira M, Huang W, Yusuff S, Shim JS, Ferrante AA, Liu JO & Parsons MJ. (2011). Chemical screen identifies FDA-approved drugs and target pathways that induce precocious pancreatic endocrine differentiation. Proc. Natl. Acad. Sci. U.S.A. , 108, 19264-9. PMID: 22084084 DOI.
- ↑ Landsman L, Nijagal A, Whitchurch TJ, Vanderlaan RL, Zimmer WE, Mackenzie TC & Hebrok M. (2011). Pancreatic mesenchyme regulates epithelial organogenesis throughout development. PLoS Biol. , 9, e1001143. PMID: 21909240 DOI.
- ↑ Dames P, Puff R, Weise M, Parhofer KG, Göke B, Götz M, Graw J, Favor J & Lechner A. (2010). Relative roles of the different Pax6 domains for pancreatic alpha cell development. BMC Dev. Biol. , 10, 39. PMID: 20377917 DOI.
- ↑ Jin ZW, Yu HC, Cho BH, Kim HT, Kimura W, Fujimiya M & Murakami G. (2010). Fetal topographical anatomy of the pancreatic head and duodenum with special reference to courses of the pancreaticoduodenal arteries. Yonsei Med. J. , 51, 398-406. PMID: 20376893 DOI.
- ↑ 7.0 7.1 Bosco D, Armanet M, Morel P, Niclauss N, Sgroi A, Muller YD, Giovannoni L, Parnaud G & Berney T. (2010). Unique arrangement of alpha- and beta-cells in human islets of Langerhans. Diabetes , 59, 1202-10. PMID: 20185817 DOI.
- ↑ Cabrera-Vásquez S, Navarro-Tableros V, Sánchez-Soto C, Gutiérrez-Ospina G & Hiriart M. (2009). Remodelling sympathetic innervation in rat pancreatic islets ontogeny. BMC Dev. Biol. , 9, 34. PMID: 19534767 DOI.
- ↑ 9.0 9.1 Kim A, Miller K, Jo J, Kilimnik G, Wojcik P & Hara M. (2009). Islet architecture: A comparative study. Islets , 1, 129-36. PMID: 20606719 DOI.
- ↑ 10.0 10.1 <pubmed>25330008</pubmed>
- ↑ <pubmed>12562919</pubmed>
Journals
- Pancreas The official journal of the American Pancreatic Association and the Japan Pancreas Society | PubMed
- Pancreatology Official Journal of the International Association of Pancreatology (IAP); European Pancreatic Club (EPC)and 16 other societies and study groups.
- Journal of the Pancreas electronic journal of pancreatology
- Diabetologia | PubMed
Online Textbooks
Endocrinology: An Integrated Approach Nussey, S.S. and Whitehead, S.A. Oxford, UK: BIOS Scientific Publishers, Ltd; 2001. table of Contents
NIH Genes & Disease Chapter 41 - Endocrine
Pathophysiology of the Endocrine System The Endocrine Pancreas
Developmental Biology (6th ed) Gilbert, Scott F. Sunderland (MA): Sinauer Associates, Inc.; c2000.
Molecular Biology of the Cell (4th Edn) Alberts, Bruce; Johnson, Alexander; Lewis, Julian; Raff, Martin; Roberts, Keith; Walter, Peter. New York: Garland Publishing; 2002. table 15-1. Some Hormone-induced Cell Responses Mediated by Cyclic AMP
Health Services/Technology Assessment Text (HSTAT) Bethesda (MD): National Library of Medicine (US), 2003 Oct.
Search NLM Online Textbooks- "pancreas development" : Endocrinology | Molecular Biology of the Cell | The Cell- A molecular Approach
Search Bookshelf Pancreas Development
Reviews
<pubmed>21084843</pubmed> <pubmed>19204986</pubmed> <pubmed>19013144</pubmed> <pubmed>18956314</pubmed> <pubmed>18676806</pubmed>
Articles
<pubmed>22110471</pubmed> <pubmed>22038519</pubmed> <pubmed>21926540</pubmed> <pubmed>21983268</pubmed> <pubmed>21537461</pubmed> <pubmed>20022941</pubmed> <pubmed>19365093</pubmed> <pubmed>19487809</pubmed> <pubmed>18810318</pubmed> <pubmed>18958289</pubmed> <pubmed>15072563</pubmed>| J Endocrinol.
Search Pubmed
Search April 2010
- Endocrine Development - All (14277) Review (4620) Free Full Text (3140)
Search Pubmed: pancreas development
Additional Images
Historic Images
External Links
External Links Notice - The dynamic nature of the internet may mean that some of these listed links may no longer function. If the link no longer works search the web with the link text or name. Links to any external commercial sites are provided for information purposes only and should never be considered an endorsement. UNSW Embryology is provided as an educational resource with no clinical information or commercial affiliation.
- F1000 Reports - Recent advances in pancreas development
- Howard Hughes Medical Institute - Seung Kim Lab
- Australian Institute of Health and Welfare - [Factsheet - Diabetic ketoacidosis (DKA) among children and young people with type 1 diabetes]
- Diabetes Australia - Type 1 Diabetes | Type 2 Diabetes
Glossary Links
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Cite this page: Hill, M.A. (2024, April 19) Embryology Endocrine - Pancreas Development. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Endocrine_-_Pancreas_Development
- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G