Ectopic Implantation Research: Difference between revisions

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==Biomedical Research Expo 2014==
''4 September 2014'' -  Wallace Wurth Building, Ground Floor Atrium - South wing. 5pm – 7pm
Come along and meet the researchers from different groups and see what Honours and Postgraduate projects are available in their labs. Discuss their research and find out more.
See you there!
:'''Links:''' [http://medicalsciences.med.unsw.edu.au/news-events/biomedical-research-expo-and-student-information-night Biomedical research expo and student information night]


==Student Ectopic Implantation Projects==
==Student Ectopic Implantation Projects==
Line 71: Line 82:
# '''Regulation by microRNA analysis''' - isolation of micro RNA from whole tube samples, (qPCR, Nanostring)
# '''Regulation by microRNA analysis''' - isolation of micro RNA from whole tube samples, (qPCR, Nanostring)
# '''Trophoblast cell function''' - growth of trophoblast cells (primary and cell lines) and analysis of trophoblast function (protein and RNA techniques shown above).
# '''Trophoblast cell function''' - growth of trophoblast cells (primary and cell lines) and analysis of trophoblast function (protein and RNA techniques shown above).
==Biomedical Research Expo 2014==
''4 September 2014'' -  Wallace Wurth Building, Ground Floor Atrium - South wing. 5pm – 7pm
Come along and meet the researchers from different groups and see what Honours and Postgraduate projects are available in their labs. Discuss their research and find out more.
See you there!
:'''Links:''' [http://medicalsciences.med.unsw.edu.au/news-events/biomedical-research-expo-and-student-information-night Biomedical research expo and student information night]





Revision as of 15:07, 3 September 2014

Introduction

Dr Mark Hill

Ectopic pregnancy is a high-risk maternal medical condition with an approximate incidence of 1.5 to 2 % in reported pregnancies. There is some indication that the incidence may be increasing (United States has increased from 4.5 per 1,000 pregnancies in 1970 to an estimated 19.7 per 1,000 pregnancies in 1992[1])

This is new collaborative research project between clinical researchers from the Women and Childrens Hospital (RHW), basic researchers from the School of Medical Scoences (SoMS), the Australian Centre for Perinatal Science (ACPS) and research support from the HSA Biobank and Bioanalytical Mass Spectrometry Facility ( BMSF).


Our research group has established a uterine tube Biobank and cell culture techniques in order to investigate factors and markers associated with tubal ectopic pregnancies.


This page introduces the project and provides some introductory information for students who may wish to carry out an Honours, Independent Research Project or higher degree in the research laboratory. Student Projects | 2014 Biomed Expo


Research Links: Dr Mark Hill | Professor William Ledger | Royal Hospital for Women | SOMS | ACPS | HSA Biobank | BMSF


Ectopic Page Links: Ectopic Implantation | Implantation | Week 2 | Placenta - Abnormalities | Trophoblast | Trophoblast - Protein Expression

Some Recent Findings

Tubal Pregnancy
More recent papers
Mark Hill.jpg
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More? References | Discussion Page | Journal Searches | 2019 References | 2020 References

Search term: Ectopic Pregnancy

<pubmed limit=5>Ectopic Pregnancy</pubmed>

Search term: Tubal Pregnancy <pubmed limit=5>Tubal Pregnancy</pubmed>

About Ectopic Pregnancy

The risk factors for tubal ectopic pregnancy include: tubal damage by infection (particularly bacterial Chlamydia trachomatis) or surgery, smoking and in vitro fertilization therapy. Prolonged tubal damage is often described as pelvic inflammatory disease and "scarring" can affect the cilia-mediated transport of the blastocyst during the first week of development. This is also the most common cause of pregnancy-related deaths in the first trimester.


A recent United Kingdom enquiry into maternal deaths[2], identified ectopic pregnancy as the fourth most common cause of maternal death (73% of early pregnancy deaths).


Ectopic sites are named according to the anatomical location: Tubal (Ampullary, Isthmic, Cornual), Cervical and Ovarian. A study of 1800 surgically treated ectopics between 1992 and 2001 identified implantation sites by frequency: interstitial (2.4%), isthmic (12.0%), ampullary (70.0%), fimbrial (11.1%), ovarian (3.2%) or abdominal (1.3%).[3]


Links: Bacterial Infection | Smoking

International Classification of Diseases

The International Classification of Diseases (ICD) World Health Organization's classification used worldwide as the standard diagnostic tool for epidemiology, health management and clinical purposes.

The two main codes cover O00 Ectopic pregnancy and O01 Hydatidiform mole.

Note that there is an additional code covering complications of the clinical treatment O08 Complications following abortion and ectopic and molar pregnancy This code is provided primarily for morbidity coding. For use of this category reference should be made to the morbidity coding rules and guidelines in Volume 2.


Links: O00 Ectopic pregnancy | O01 Hydatidiform mole | Chapter XV Pregnancy Childbirth | International Classification of Diseases


Biomedical Research Expo 2014

4 September 2014 - Wallace Wurth Building, Ground Floor Atrium - South wing. 5pm – 7pm

Come along and meet the researchers from different groups and see what Honours and Postgraduate projects are available in their labs. Discuss their research and find out more.


See you there!


Links: Biomedical research expo and student information night

Student Ectopic Implantation Projects

This page introduces the project and provides some introductory information for students who may wish to carry out an Honours, Independent Research Project or higher degree in the research laboratory.


The projects use first trimester ectopic implantation and normal uterine tube material analysis/comparison. Studies include internal controls with comparison to uterine tube outside the site of implantation in the same tube. The projects include some types of the methods used and trained for within the projects.


  1. Protein analysis - isolation of protein from whole tube samples, quantification/quality and analysis (Western blot, ELISA, immunochemistry, microarray, Proteomics)
  2. Gene expression analysis - isolation of messenger RNA (mRNA) from whole tube samples, quantification/quality and analysis (Northern blot, qPCR, in situ hybridisation, Nanostring)
  3. Regulation by microRNA analysis - isolation of micro RNA from whole tube samples, (qPCR, Nanostring)
  4. Trophoblast cell function - growth of trophoblast cells (primary and cell lines) and analysis of trophoblast function (protein and RNA techniques shown above).


Other Projects

Human Embryo Collections - analysis and digital databank development of material collected from a number of European and United States human embryo collections.


Background Information

Protein Analysis - Quantitative Proteomics

Isobaric tags for relative and absolute quantitation (iTRAQ) allows tandem mass spectrometry identification and quantification of proteins.[4][5]

Liquid chromatography–mass spectrometry (LCMSMS)

Protein Pilot software allows protein identification and quantitation, identify hundreds of peptide modifications and non-tryptic cleavages simultaneously. Software allows distinguishing protein isoforms, protein subsets, and suppress false positives, as well as visualize peptide-protein associations and relationships.


Links: BMSF | Protein Pilot


RNA Analysis

Clinical

Diagnosis is generally by serial beta Human Chorionic Gonadotropin (hCG) measurements and transvaginal ultrasound (TVS) evaluation.


Links: Human Chorionic Gonadotropin | Ultrasound

Salpingectomy

Salpingectomy is a clinical term for the surgical removal of a single or both uterine tube(s)

Salpingotomy is a clinical term for surgically creating an opening into the uterine tube, to remove an ectopic pregnancy, leaving the tube.[6][7]


Methotrexate

(MTX, amethopterin) Drug with several different uses including the treatment of ectopic pregnancy[8] and for the induction of medical abortions. Acts as a antimetabolite and antifolate (folic acid antagonist) drug that inhibits DNA synthesis in actively dividing cells, including trophoblasts, and therefore has other medical uses include cancer and autoimmune disease treatment. Treatment success in ectopic pregnancy relates to serum β human chorionic gonadotropin (β-hCG) concentration.

  • Comparison of double- and single-dose methotrexate protocols for treatment of ectopic pregnancy (EP)[9]


Links: Medline Plus

References

  1. <pubmed>7823895</pubmed>
  2. Confidential Enquiry into Maternal Deaths (CEMD) Why Mothers Die 2000–2002 PDFPDF2
  3. <pubmed>12456628</pubmed>
  4. <pubmed>17177251</pubmed>
  5. <pubmed>22451173</pubmed>
  6. <pubmed>24969151</pubmed>
  7. <pubmed>24499812</pubmed>
  8. <pubmed>8317518</pubmed>
  9. <pubmed>22035883</pubmed>

Reviews

<pubmed>20071358</pubmed> <pubmed>20023297</pubmed> <pubmed>16595714</pubmed>

Articles

<pubmed>7194809</pubmed> <pubmed>19978839</pubmed>

Search Pubmed

Search Pubmed: ectopic pregnancy | ectopic implantation | tubal pregnancy | tubal implantation


External Links

External Links Notice - The dynamic nature of the internet may mean that some of these listed links may no longer function. If the link no longer works search the web with the link text or name. Links to any external commercial sites are provided for information purposes only and should never be considered an endorsement. UNSW Embryology is provided as an educational resource with no clinical information or commercial affiliation.


Glossary Links

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Cite this page: Hill, M.A. (2024, March 29) Embryology Ectopic Implantation Research. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Ectopic_Implantation_Research

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© Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G