Computed Tomography: Difference between revisions

Revision as of 11:30, 17 August 2010

Introduction

Micro-computed tomography apparatus

Computed Tomography or computed axial tomography (CAT or CT scan) began in 1970's using x-ray and a computer to produce images either as individual slices or reconstructed to give three dimensional (3D) views of specific anatomical regions or structures.

In embryology, the technique of micro-CT (μCT) has recently begun to become relevant in the analysis of normal development features and those seen in genetically modified animal models, such as the mouse (More? Mouse Development).

Other potential developmental research imaging techniques include: positron emission tomography (PET), single photon emission computed tomography, magnetic resonance imaging, computed tomography, optical bioluminescence, fluorescence and high frequency ultrasound.

Links: Flash - Computed Tomography | Quicktime - Computed Tomography | Category:Computed Tomography | original page

Some Recent Findings

Micro-computed tomography-based phenotypic approaches in embryology: procedural artifacts on assessments of embryonic craniofacial growth and development.^[1] "we assessed the nature and degree of morphological artifacts attending μCT scanning following use of common fixatives, using a two dimensional (2D) landmark geometric morphometric approach to track the accumulation of distortions affecting the embryonic head from the native, uterine state through to fixation and subsequent scanning. ...The degree to which artifacts are introduced in the generation of random craniofacial shape variation relates to the degree of specimen dehydration during the initial fixation. "

Rapid Three-Dimensional Phenotyping of Cardiovascular Development in Mouse Embryos by Micro-CT with Iodine Staining.^[2] "Our method benefits from the ease of sample preparation, low toxicity, and low cost. Furthermore, we show how multiple cardiac defects can be demonstrated by micro-CT in a single specimen with a known genetic lesion. Indeed, a previously undescribed cardiac venous abnormality is revealed in a PlexinD1 mutant mouse."

Virtual histology of transgenic mouse embryos for high-throughput phenotyping.^[3] "We present a novel, rapid, and inexpensive method for obtaining high-resolution virtual histology for phenotypic assessment of mouse embryos. Using osmium tetroxide to differentially stain tissues followed by volumetric X-ray computed tomography to image whole embryos, isometric resolutions of 27 mum or 8 mum were achieved with scan times of 2 h or 12 h, respectively, using mid-gestation E9.5-E12.5 embryos."

Early Mouse Development MicroCT

Mouse E9.5^[3]
Mouse E10.5^[3]
Mouse E10.5 head ^[1]
Mouse E11.5^[3]
Mouse E11.5^[3]
Mouse E12.5^[3]
Mouse E14.5^[4]

Movies

	Flash version Quicktime version
Mouse E11.5 microCT scan^[5]

Links: Flash - Computed Tomography | Quicktime - Computed Tomography

References

↑ ^1.0 ^1.1 <pubmed>20163731</pubmed>|BMC Developmental Biology
↑ <pubmed>20190279</pubmed>
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 <pubmed>16683035</pubmed>| PLoS Genetics
↑ <pubmed>18713865</pubmed>| PNAS
↑ <pubmed>16683035</pubmed>

Search Pubmed

Search Pubmed: Embryo Computed Tomography | Computed Tomography | Micro-computed tomography apparatus

External Links

Duke Center for In Vivo Microscopy A 4D Atlas and Morphologic Database
- Normal C57BL/6 mouse embryos from embryonic day E10.5 to E19.5
- Normal C57BL/6 mouse neonates from post-natal day 0 to 32
- Mutant mouse embryos with cardiac septation defects (conditional ablation of the Smoothened receptor gene, Mef2C-AHF-Cre;Smo^{flox/-^mutants)}
University of Calgary 3D Morphometrics Lab

Glossary Links

Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | Numbers | Symbols | Term Link

Cite this page: Hill, M.A. (2024, April 19) Embryology Computed Tomography. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/Computed_Tomography

What Links Here?

[PMID20163731-1] 1.0 ^1.1 <pubmed>20163731</pubmed>|BMC Developmental Biology

[2] <pubmed>20190279</pubmed>

[PMID16683035-3] 3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 <pubmed>16683035</pubmed>| PLoS Genetics

[4] <pubmed>18713865</pubmed>| PNAS

[5] <pubmed>16683035</pubmed>

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[2]

[3]

[4]

[5]

@@ Line 17: / Line 17: @@
 * '''Rapid Three-Dimensional Phenotyping of Cardiovascular Development in Mouse Embryos by Micro-CT with Iodine Staining.'''<ref><pubmed>20190279</pubmed></ref> "Our method benefits from the ease of sample preparation, low toxicity, and low cost. Furthermore, we show how multiple cardiac defects can be demonstrated by micro-CT in a single specimen with a known genetic lesion. Indeed, a previously undescribed cardiac venous abnormality is revealed in a PlexinD1 mutant mouse."
-* '''Virtual histology of transgenic mouse embryos for high-throughput phenotyping.'''<ref><pubmed>16683035</pubmed>| [http://genetics.plosjournals.org/perlserv/?request=get-document&doi=10.1371/journal.pgen.0020061 PLoS]</ref> "We present a novel, rapid, and inexpensive method for obtaining high-resolution virtual histology for phenotypic assessment of mouse embryos. Using osmium tetroxide to differentially stain tissues followed by volumetric X-ray computed tomography to image whole embryos, isometric resolutions of 27 mum or 8 mum were achieved with scan times of 2 h or 12 h, respectively, using mid-gestation E9.5-E12.5 embryos."
+* '''Virtual histology of transgenic mouse embryos for high-throughput phenotyping.'''<ref name=PMID16683035><pubmed>16683035</pubmed>| [http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0020061 PLoS Genetics]</ref> "We present a novel, rapid, and inexpensive method for obtaining high-resolution virtual histology for phenotypic assessment of mouse embryos. Using osmium tetroxide to differentially stain tissues followed by volumetric X-ray computed tomography to image whole embryos, isometric resolutions of 27 mum or 8 mum were achieved with scan times of 2 h or 12 h, respectively, using mid-gestation E9.5-E12.5 embryos."
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