Difference between revisions of "American Journal of Anatomy 5 (1906)"

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=THE AMERICAN JOURNAL OF ANATOMY=
 
=THE AMERICAN JOURNAL OF ANATOMY=
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THE AMERICAN JOURNAL
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OF
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ANATOMY
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EDITORIAI. BOARD
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CHARLES R. BAUDEEN,
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Universitij nf Wisco>ni)i.
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HENRY H. DONALDSON,
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Wisiar Institute of Aiiatotiiy .
 +
THOMAS DWIGHT,
 +
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Harvard Univemitij.
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JOSEPH MARSHALL FLINT,
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University of California.
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SIMON H. GAGE,
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Cornell Universitij.
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G. CARL HUBER,
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University of Jlichiyan.
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GEORGE S. HUNTINGTON,
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Columbia University.
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FRANKLIN P. MALL,
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Johns Hopkins University .
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J. PLAYFAIR McMURRICH,
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University of Jfic/iiyaii.
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CHARLES S. MINOT,
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Harvard University.
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GEORGE A. PIERSOL,
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University of Pennsylvania.
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HENRY McE. KNOWER, Secuetaky,
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Johns Hopkins University.
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VOLUME V
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J 906
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THE AMERICAN JOURNAL OF ANATOMY
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BALTIMORE, MD., U. S. A.
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Zf)t fviiUnvoaii Company
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BALTIMORE, MD., V- 8. A.
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/a ^»
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CONTENTS OF VOL V
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No. 1. December 1, 1905.
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I. John Warken. The Developnicnt of the Paraj^hysis and
 +
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the Pineal liegion in Nectiinis Maculatus .... 1
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With 23 text figures.
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II. E. T. Bell. The Development of the Thymus ... 39
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With 3 plates and 5 text figures.
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III. Jeremiah 8. Ferguson. The A^eins of the Adrenal. . G3
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With 3 text figures.
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IV. George Walker. The Blood Vessels of the Prostate
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Gland 73
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With 3 colored plates.
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V. Bennet M. Allen. The Emhryonic Development of the
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Pete-Cords and Sex-Cords of Chrysemys 79
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Witli 1 doul)]e plate and G text figures.
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VI. Frederic T. Lewis. The Development of the Lymphatic
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System in Pabbits 95
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With 8 text fi,gures.
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VII. Frederic T. Lewis. The Development of the Veins in
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the Limbs of Rabbit Embryos 113
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With 1 text figure.
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No. 2. May 31, 1906.
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A'lTI. Poss Granvtllk IIarhison. Further Experiments on the
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Development of Peripheral Nerves 131
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With 5 figures.
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iv Contciils
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IX. Ai.i!i:i;t C. Eyclesiiymi:!; .-iiid James Meredith Wilson.
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The (lastnilation and I'iinbrvo Formation in Amia
 +
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C'alva 13;i
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With I (lonl.lc plalcs.
 +
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X. Charles F. W. McClure. A Contribution to the Anatomy
 +
and Development of the Venous System of Didelpliys
 +
Marsiii)ialis (L.)-— I'iH-t II. Development .... lOo
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Willi 1 single and 4 double plates and 27 text fi2:ures.
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LIST OF MEMBERS OF ASSOCIATION OF AMERICAN ANATOMISTS XXII-XXXII
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No. 3. July 25, 190G.
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XL Franklin P. Mall. A Study of the Structural Unit of
 +
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the Liver ^ 227
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With 74 fio-ures and 7 tables.
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XII. Albert C. Eycleshy'mer. The Development of Clnoma
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tophores in Necturus 309
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With 7 figures.
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XIII. Sidney Klein, S. M., ]\L D. On the X^ature of the
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Granule Cells of Paneth in the Intestinal Glands of
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Mammals 315
 +
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AVith .5 figures.
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XIV. Charles L. Edwards and Clarence W. LTahn. Some
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Phases of the Gastrulation of the Horned Toad, Phrynosoma Cornutum Harlan 331
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With 15 text figures.
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No. 4. September 1, 1906.
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XV. Robert Bennett Bean. Some Racial Peculiarities of
 +
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the Negro Brain ... 353
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With 16 fig-ures, 12 charts, and 7 tables.
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Contents ' v
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XVT. Franklin P. Mall. On Ossification Centers in Human
 +
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Embryos Less Than One Hnndred Days Old 433
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With (i text figures and 7 tables.
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XVII. J. L. Bremer. Description of a 4-inm. Human Embryo. 459
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With 16 text figures.
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XVIII. Charles R. Stockard. The Development of tlio Mouth
 +
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and Gills in Bdellostoma Stouti 481-517
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With 36 figures.
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XIX. PROCEEDIXGS OF THE AS80CIATI0X OF
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AMERICAN ANATOMISTS. NINETEENTH SESSION, August 6-10, 1905. TWENTIETH SESSION,
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Decemher 27, 28, and 29, 1905 I-XX
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THE DEVELOPMENT OF THE PARAPHYSIS AND THE
 +
PINEAL REGION IN NECTURUS MACULATUS. '
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BY
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JOHN WARREN.
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Demonstrator of Anatomy, the Anatomical Laboratory, Harvard Medical
 +
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School.
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With 23 Text Figures.
 +
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The presence of the paraphysis in Necturns was noted by Prof. C. S.
 +
Minot in his article " On the Morphology of the Pineal Region, based on
 +
its Development in x'Vcanthias " (38), and a brief description of certain
 +
stages given. C. L. Herrick (15, PI. YIII, Fig. 1, 3, 4) gives a brief
 +
account of the adult paraphj'sis, and shows it in the above figures, vvlicre
 +
it is named " Preparaphysis." Osbom (31, PI. IV) shows th-e paraphysis in an adult brain in comparison with the brains of other amphibia. Kingsbury (21) describes briefly the adult paraphysis as well
 +
as a few of the earlier stages, and also gives an account of the epi))hysis
 +
and the plexuses. I have found, however, no detailed account of all the
 +
stages in the development of the paraphysis and the pineal region. This
 +
term is used here in the same sense as in Minot's article, quoted above.
 +
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The greater part of the specimens studied for this article were taken
 +
from the Embryological Collection of the Harvard Medical School, and
 +
the numbers of each section used are given. Other specimens were prepared specially for this purpose. In some cases where the plane of section was uneven, two or more sections were used in drawing the figures
 +
ill order to show all the structures, which should appear in the uK^dian
 +
line.
 +
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Fig. 1 is a median sagittal section through the brain of an einl)ryo of
 +
8-9 mm. I am indebted to a colleague for the drawing of this section,
 +
as this stage is wanting in the collection. In the roof of the fore brain
 +
three arches are seen. From before backward these are the j)aia|)liysal
 +
arch, P. A., the post- velar arch, P. V. A., and the epiphysal arch, Ep. A.
 +
The first two are separated by the velum transversum. V, which marks
 +
the limit between the two subdivisions .of the fore brain. Hence the
 +
paraphysal arch belongs to the telencephalon, the other two to the
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AMERICAN' JOUIJNAL OF ANATOMY. VOL. V.
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1
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2 Paraphysis and the Pineal Ees^ion in Necturns Maenlatus
 +
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dieneephalon. The epiphysal are]i is homided l)y two angles, which represent the position of the future supra and posterior commissures. The
 +
velum transversum is a simple infolding of the brain roof, and consists
 +
of two distinct layers, one caudad and one cephalad, the space between
 +
them being filled by a loose mesenchymal tissue, which later contains
 +
numerous blood vessels. This figure is practically identical with Minot's
 +
figure of acanthias of the same stage (28, Fig. 1), and is, therefore, of
 +
great importance in showing the homologies of these parts in elasmobranchs and amphibians. It is probable, as Minot states, that these
 +
arches occur in most of the vertebrate series.
 +
 +
The term post-velar arch, introduced by Minot (28), is much better
 +
for purposes of description than the terms " zirbelpolster " of German
 +
writers, and the " dorsal sack " or " postparaphysis " of American authors.
 +
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 +
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Dien
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Fig. 1.
 +
 +
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Fig. 2.
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Fig. 1. Embryo of 8-9 mm. Sagittal section, X 63 diams.
 +
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Fig. 2. Embryo of 10 mm. Harvard Embryological Collection, Sagittal
 +
Series, No. 269, Section 39, X 63 diams.
 +
 +
 +
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Fig. 2 represents the roof of the dieneephalon and telencephalon of
 +
an embryo of 10 mm. The two layers of the velum are nearer together
 +
and in the region of the epiphysal arch are seen the first signs of the
 +
epiphysis, E. This structure is a small rounded diverticulum, which
 +
arises from the cephalic end of the arch. It is hollow and opens into
 +
the cavity of the fore brain.
 +
 +
Fig. 3 is a similar section of an embryo of 12 mm. The velum is a
 +
trifle longer and the epiphysis a little larger than in the preceding figure.
 +
Immediately cephalad to the velum a very small evagination in the
 +
paraphysal arch can be seen, P. This is the first sign of the paraphysis,
 +
and it appears distinctly later than the epiphysis. The latter overlaps
 +
its short stalk both caudad and cephalad, and at this stage the stalk is
 +
still hollow, though its cavity was obliterated in this section.
 +
 +
 +
 +
John Warren 3
 +
 +
Fig. 4 is a section of an embryo of 13 mm. The velum is again a
 +
little longer and its caudal layer is now distinctly thinner than its cephalic
 +
layer. The paraphysis is now a well-marked narrow diverticulum extending dorsad from the paraphysal arch parallel to the velum. The
 +
paraphysal arch just cephalad to the opening of the paraphysis has been
 +
 +
 +
 +
 +
Tel.
 +
 +
 +
 +
 +
Fig. 3.
 +
 +
 +
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Pig. 4.
 +
 +
 +
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Fig. 3. Embryo of 12 mm. Harvard Embryological Collection, Sagittal
 +
Series, No. 49, Section 58, X 63 diams.
 +
 +
Fig. 4. Embryo of 13 mm. Harvard Embryological Collection, Sagittal
 +
Series, No. 598, Sections 71 and 75, X 63 diams.
 +
 +
forced downward to a slight degree, as there is relatively more space
 +
between it and the ectoderm than in the previous figures. The epiphysis
 +
is about the same size as in Fig. 3, and its opening into the brain is
 +
clearly seen.
 +
 +
Fig. 5 is a section of an embryo of 12A mm., which is, however, further
 +
advanced than that of Fig. 4. The velum, the post-velar arch, and the
 +
epiphy.sis are about the same, but the paraphysis is distinctly longer, and
 +
 +
 +
 +
 +
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Fig. 5. Embryo of 12.4 mm. Harvard Embryological Collection, Sagittal
 +
Series, No. 675, Section 57, X 63 diams.
 +
 +
Fig. 6. Same as Fig. 5, X about 120 diams.
 +
 +
has become a narrow tube. The brain roof cephalad to it has descended
 +
still more into the cavity of the telencephalon and the opening of the
 +
paraphysis is much nearer the tip of the velum. Fig. 6 is the same
 +
section as Fig. 5, only drawn on a higher scale to show the histological
 +
details. The walls of the paraphysis and "velum consist of a single layer
 +
of cells, with large oval nuclei and without very distinct cell boundaries.
 +
 +
 +
 +
4 rai;i])li\si> and the Pineal Kegion in Necturus Maculatus
 +
 +
These cells are, of course, continuous with tliose which form the brain
 +
wall in this region. The same is true of the epiphysis, but the walls seem
 +
thicker, as the organ has been cut somewhat obliquely. Close to the
 +
paraphysis two vessels can be seen, a larger one cephalad and a much
 +
smaller one caudad, _ F<?6'. The vessels lie in intimate relation to this
 +
structure, and it is important to note their relation at this early stage,
 +
because as development progresses the relation between paraphysis and
 +
blood vessels becomes more and more intimate.
 +
 +
Fig. 7 is a section of an embryo of 15 mm. The most striking feature
 +
here is the increase in size of the paraphysis. which has become a long
 +
tube with a lumen extending its entire length, and at its distal end a lateral diverticulum has appeared. The roof of the fore brain has now
 +
descended to such a degree that the opening of the paraphysis is on a level
 +
with the tip of the velum. The velum itself has lost its cephalic layer,
 +
and consists of one .layer only, which, however, is much longer than the
 +
velum in Fig. 5. If Figs 4, 5, and 7 are compared it will be seen that
 +
 +
 +
 +
 +
Tel.
 +
 +
 +
 +
Fig. 7. Embryo of 15 mm. Harvard Embryological Collection, Sagittal
 +
Series. No. 79, Sections 85 and 89, X 63 diams.
 +
 +
the distal end of the paraphysis is practically at the same distance from
 +
the ectoderm in each case. As the paraphysis has developed during those
 +
stages into a long tube, its growth must have occurred by a downward
 +
extension of the neighboring parts into the cavity of the fore brain. This
 +
is practically the same process described by Minot in Acanthias. It is
 +
also shown by the great increase in distance between the roof of the
 +
telencephalon and the ectoderm from Fig. 4 to Fig. 7. The opening of
 +
the paraphysis in Fig. 3 is nearly on a level with tlie base of the velum,
 +
and as the down growth of the parts takes place the opening of the paraphysis and the paraphysal arch descend, apparently pushing the cephalic
 +
layer of the velum ahead of them. Therefore the single layer of the
 +
velum in Fig. 7 really corresponds to the original caudal layer, plus the
 +
cephalic layer, which has been forced down ahead of the opening of the
 +
paraphysis.
 +
 +
In stud5''ing Fig. 7 it might seem as if the posterior wall of the paraphysis corresponded to the cephalic layer of the velum. This, however, is
 +
 +
 +
 +
John Warren 5
 +
 +
not tlie case, as can be seen in a wax reconstruction of the parts, Fig. S.
 +
This is a reconstruction of the brain of an embryo of 14.5 mm. The tops
 +
of the hemispheres, H, have been removed to give a clear view of the
 +
paraphysis, P, which otherwise would be more or less covered in by them.
 +
The paraphysis appears as a straight tube in the median line and caudad
 +
to it is seen a broad partition, V , extending the whole width of the diencephalon. This is the velum, consisting of one layer only, which represents the tw^o originally distinct cephalic and caudal layers. The down
 +
grow^th of the parts in order to provide room for the development of the
 +
paraphysis has formed a deep angle in the roof of the fore brain. This
 +
 +
 +
 +
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Fig. 8. Wax model of brain of embryo of 14.5 mm. Harvard Embryological Collection, Sagittal Series, X 120 times.
 +
 +
angle is bounded caudad by the velum and cephalad or ventrad by the
 +
narrow roof of the telencephalon (paraphysal arch) immediately
 +
cephalad to the paraphysis. As the hemispheres develop, they grow at
 +
first in a dorsal direction and occupy the space left by the formation of
 +
this angle, so that the paraphysis is practically buried between the hemispheres in front and the velum behind. Fig. 10. The growth of the
 +
paraph3^sis must, therefore, be regarded as liaving an important effect on
 +
the development of the fore brain at this stage.
 +
 +
Up to this stage the development of the velum has been in a ventral
 +
direction towards the floor of the fore brain, but now it begins to grow in
 +
quite a ditferent direction. In Fig. 7 a distinct bulging of the velum is
 +
 +
 +
 +
6 Paraphysis and tlio Pineal Pogion in Xeclurus 'Maculatus
 +
 +
seen, which is extending candad at ncarl}- a right angle to its previous
 +
line of growth. If the roof of the telencephalon be closely examined a
 +
slight bulging will be seen just cephalad to the opening of the paraphysis.
 +
These two outgrowths into the fore brain mark the beginning of the
 +
choroid plexuses, which, therefore, have in their origin a very intimate
 +
and definite relation to the opening of the paraphysis, one arising caudad
 +
and the other cephalad to it. The epiphysis at this stage has increased
 +
considerably in size, and the cavity in its stalk is now permanently obliterated. The body of the organ overlaps the stalk a little behind, and is
 +
beginning to grow well forward of it. The posterior commissure, P. C,
 +
appears here for the first time, a distinct interval in the roof of the brain
 +
lying between it and the stalk of the epiphysis.
 +
 +
 +
 +
 +
Hyp.
 +
 +
 +
 +
OCh.
 +
 +
 +
 +
A.C.
 +
 +
 +
 +
Fig. 9. Embryo of 17.5 mm. Harvard Embryological Collection,
 +
Series, No. 540, Sections 113-115, X 63 diams.
 +
 +
 +
 +
Sagittal
 +
 +
 +
 +
Fig. 9 is a section through the brain of an embryo of 17.5 mm. The
 +
paraphysis has increased in length, and from its distal end, which is
 +
somewhat enlarged, small tubules are given off. The whole tube is tipped
 +
somewhat forward. The choroid plexus is now well developed, and consists of two distinct parts, one dorsal and one ventral. The dorsal part
 +
corresponds to the velum, which has grown caudad as far as the mid brain
 +
and has absorbed a large part of the post-velar arch. The ventral part is
 +
developed from the original paraphysal arch, and is growing towards the
 +
floor of the fore brain. Burckhardt (3) refers to these plexuses as
 +
" plexus medius " and " plexus inferioris," respectively, and Mrs. Gage
 +
(13), who studied them in Diemyctylus, where the anatomical conditions
 +
closely resemble those of JSTecturus, names them the " diaplexus " and
 +
" prosoplexus." Prof. Minot has suggested the terms diencephalic
 +
 +
 +
 +
John Warren 7
 +
 +
plexus for the dorsal part, and tel encephalic plexus for the ventral part,
 +
and I shall use these terms, as they express more clearly the exact origin
 +
of each plexus.
 +
 +
The diencephalic plexus, V. Plx., appears as a large wedge-shaped mass
 +
covered by a thin layer of cells, and consisting of a loose connective tissue
 +
in the interstices of which numerous blood corpuscles can be seen. The
 +
telencephalic plexus, Tel. Plx., has the same general characteristics as
 +
the diencephalic. The epiphysis has become flattened and more elongated, and is attached by a narrow stalk to the brain wall. The supra
 +
commissure, S. C, is seen just cephalad to the stalk of the epiphysis,
 +
which is prolonged forward above it. I was unable to obtain any sagittal
 +
series between 15 and 17.5 mm., but in a transverse series of 16.5 mm. the
 +
first traces of this commissure can just be made out. and therefore it
 +
 +
 +
 +
 +
Fig. 10. Wax model of brain of embryo of 18 mm. Harvard Embryological Collection, Frontal Series, No. 850, X about 75 diams.
 +
 +
 +
 +
probably appears between 16 mm. and 17 mm. as a rule, but at these early
 +
stages there is a good deal of variation in the development of all these
 +
parts. The posterior commissure is rather larger than in the previous
 +
stage.
 +
 +
Fig. 10 is the drawing of the model of the brain of an embryo of
 +
18 ram. This model is intended to show the circulation of the paraphysis
 +
at this stage. The distal end of the paraphysis, P, is surrounded by a
 +
venous circle, from either side of which veins, Ves., run outward and backward just caudad to the hemispheres, H, to terminate in the internal
 +
jugular vein, I. J. V. This vein is passing backward external to the fifth,
 +
V, and seventh, VII, cranial nerves. Fig. 6 showed the intimate relation
 +
of the paraphysis to these vessels at 12.4 mm., and when the sections of
 +
this series were followed out it was found that here the vessels surrounded the tip of the paraphysis. It seems that as the paraphysis devel
 +
 +
 +
S Parapliv.sis and the Pineal Region in Nectnms Maculatus
 +
 +
ops it forces its way into the veins lying over this part of the fore brain,
 +
and the tnbnles, as they are given off at la.ter stages, force their way into
 +
these veins, Fig. 15, forming the sinusoidal type of circulation described
 +
by Minot (29) and Lewis (25). From the venous circle shown in Fig. 10
 +
smaller vessels run down along the sides of the paraphysis and anastomose with the vessels of the choroid plexuses. A vessel also runs back to
 +
the epiphysis, and a larger one forward between the hemispheres. The
 +
circulation of this region appears at this stage to be mostly venous, as I
 +
could trace the arteries only to their point of entrance in the anlage of
 +
the skull, and the return circulation probably occurs by means of a
 +
minute capillary network over the surface of tlie brain.
 +
 +
Fig. 11 is a section through the brain of an embryo of 26 mm. The
 +
 +
 +
 +
 +
Fig. 11. Embryo of 26 mm. Harvard Embryological Collection, Sagittal
 +
Series, No. 377, Sections 125 and 126, X 63 diams.
 +
 +
 +
 +
paraphysis here is much more developed. It inclines somewliat forward,
 +
and from its wide central lumen a number of tubules are given oif in
 +
every direction. The epiphysis and the commissures show but little
 +
change. The striking feature of this figure is the great development of
 +
the plexuses. The diencephalic plexus, D. Pl.r.. has grown through the
 +
mid-brain nearly to the hind-brain, and the telencephalic plexus, Tel.
 +
Fix., has grown downwards into the depths of the cavity of the fore brain
 +
towards the infundibular recess.
 +
 +
Fig. 12 is a transverse section of an embryo of 26 mm., corresponding
 +
approximately to the line A-B, Fig. 11. The section passes through the
 +
epiphysis, E, and the supra commissure, S. C, just beneath it. Then
 +
through the diencephalic plexus, D. Fix., and that part of the cavity of
 +
the diencephalon between this plexus and the roof, T>ien. The section
 +
 +
 +
 +
John Warrcu 9
 +
 +
then passes through the paraphysis at a point where two small tubules are
 +
given off, then through the telencephalic plexus, Te2. Plx.. the telen
 +
 +
 +
 +
FiG. 12. Embryo of 26 mm. Harvard Embryological Collection, Transverse Series, No. 376, Section 89, X 63 diams. (See line A-B, Fig. 11.)
 +
 +
cephaloii, Teh. the lateral ventricles, L. V., and the foramina of Munro,
 +
F. M. In this section the plexuses of tlie hemispheres, L. Plx., are seen.
 +
They arise on either side of the origin of the telencephalic plexus, and
 +
 +
 +
 +
 +
jRi-TT
 +
 +
 +
 +
Fig. 13. Embryo of 26 mm. Harvard Embryological Collection, Frontal
 +
Series, No. 378, Section 138, X 63 diams. (See line C-D, Fig. 11.)
 +
 +
pass outward at right angles to it through the foramina o£ Munro into the
 +
lateral ventricles.
 +
 +
Fig. 13 is a frontal section through an embryo of 2Q mm., corresponding
 +
 +
 +
 +
10
 +
 +
 +
 +
Paraphysis aud the Pineal Kegion in Necturus Maculatus
 +
 +
 +
 +
closely to the line C-D, Fig. 11. The section passes through the paraphyses, P, and a large lateral tubule, and then through the entire length
 +
 +
 +
 +
 +
Fig. 14. Same Series as Fig. 13. Section 108. (See line E-F, Fig. 11.)
 +
 +
of the diencephalic plexus, D. Fix., the distal end of which is here enlarged and has reached to the hind brain, H. B. Fig. 14 is of the same
 +
 +
,Tf,s-.
 +
 +
 +
 +
M.
 +
 +
 +
 +
 +
 +
 +
D.PIX
 +
 +
 +
 +
o??,:
 +
 +
 +
 +
^ m
 +
 +
 +
 +
<£5S^ferffiICi^!^^^;
 +
 +
 +
 +
 +
Tel.Plx. .^1-^^
 +
 +
 +
 +
 +
Fig. 15. Same as Fig. 11. X about 150 diams.
 +
 +
series as Fig. 13, and corresponds approximately to the line E-F, Fig. 11.
 +
It passes through the telencephalic plexus, Tel. Fix., the plexus of the
 +
 +
 +
 +
John Warren
 +
 +
 +
 +
11
 +
 +
 +
 +
hemispheres. L. Plx., and the lateral ventricles, L. V. It shows clearly
 +
how the plexuses of the hemispheres arise from the telencephalic plexus
 +
and pass at first outward and then forward through the foramina of
 +
Munro towards the cephalic extremity of the lateral ventricles.
 +
 +
Fig.- 15 is a high power drawing of Fig. 11, magnified 150 diams.
 +
The wall of the paraphysis consists of a single layer of cells with large
 +
oval nuclei, and these cells are continuous with the cells covering the
 +
choroid plexuses, but the latter are flatter and form a thinner layer. On
 +
either side of the paraphysis two large vessels are seen, ves., the epithelial
 +
cells of which lie directly against the wall of the paraphysis. The little
 +
tubules seem to be forcing their way into these vessels, which are branches
 +
of the vessels seen in Fig. 6 and Fig. 10. The vessels also pass down into
 +
the choroid plexuses. Fig. 16 is a section of an embryo of 31.4 mm.
 +
 +
 +
 +
 +
HB.
 +
 +
 +
 +
OCh.
 +
 +
 +
 +
Hyp.
 +
 +
 +
 +
Fig. 16. Embryo of 31.4 mm. Harvard Embryological Collection, Sagittal
 +
Series, No. 537, Sections 119-122, X 63 dlams.
 +
 +
 +
 +
The general arrangement is practically the same as in Fig. 11, except that
 +
all the parts have progressed somewhat in their development. The distal
 +
end of the paraphysis has begun to grow distinctly more cephalad, and the
 +
whole structure is much larger than at 26 mm. The choroid plexuses are
 +
more extensive, and from the diencephalic plexus a prolongation is extending downwards towards the telencephalic plexus. This latter has
 +
pretty well filled up the depths of the third ventricle, and from it prolongations dip down into the recesses in the floor of the fore brain. The
 +
two commissures are practically the same as they were at 26 mm., and
 +
 +
 +
 +
12 Paraph vsis and the I'iiieal Heo-ion in Noetiiriis Maeulatus
 +
 +
 +
 +
though the epiphysis is a little larger it has been displaced considerably
 +
candad, as this part of these sections was unluckily somewhat injured.
 +
 +
Fig. IT is a section through the
 +
brain of an adult necturus. This
 +
drawing is magnified 38 diams. only,
 +
as it was too large to draw on the
 +
same scale as the preceding figures.
 +
The paraphysis, P. forms a very
 +
complex structure extending far forward above and between the hemispheres. It consists in a general Avay
 +
of a proximal and a distal part. The
 +
former is broad and thick, and oxtends forward and upward. It then
 +
turns forward at quite a marked
 +
angle to form the distal part, which
 +
is narrow and. tapering. The central
 +
canal in the proximal part is very
 +
large and irregiilar, but in the distal
 +
portion much narrower. From all
 +
parts of this canal a large number of
 +
tubules are given off, which extend
 +
in every direction, and between
 +
which lies a confused mass of bloodvessels. One sees here a large vessel
 +
ventrad to the organ, and a smaller
 +
dorsad to it. the same relations as
 +
appear at 12.4 mm., Fig. 6. From
 +
these vessels branches pass into the
 +
choroid plexuses.
 +
 +
Fig. 18 is a transverse . section of
 +
an adult brain corresponding approximately to the line A-B, Fig. 17.
 +
This is drawn on the same scale as
 +
most of the preceding figures, 63
 +
diams. The paraphysis, P, is seen in
 +
the median line between the hemispheres. It shows a distinct central
 +
eavit}', with many tubules running out in every direction, between which
 +
is a mass of blood-vessels of all sizes. Below a portion of the telencephalic plexus aiu1 the ])l(\\-us of the hemispheres are seen.
 +
 +
 +
 +
 +
Via. 17. Brain of adult necturus.
 +
Sag-ittal Section, x 38 diams.
 +
 +
 +
 +
John Warroii
 +
 +
 +
 +
13
 +
 +
 +
 +
 +
Fig. is. Brain of adult necturus. Transverse section, X 63 diams. (See
 +
line A-B. Fig. 17.)
 +
 +
 +
 +
14 Paraphysis and the Pineal Eegion in Nectunis Maciilatus
 +
 +
Fig. 19 is from a wax recoustruction of tlie adult paraphysis on a
 +
scale of 120 diams., made from the same series as Fig. 18. The angle
 +
between the proximal and distal parts is quite striking, and is much more
 +
marked in Ichthyophis (Burckhardt, 4, Fig. 1), but of course this division
 +
into proximal and distal parts is really a purely arbitrary one. This
 +
model gives a good idea of the complex structure of the organ. The
 +
tubules are of all shapes and sizes, often convoluted and anastomosing
 +
with each other. The spaces between them, which the vessels occupy, are
 +
quite large and striking.
 +
 +
 +
 +
 +
Fig. 19. Wax model of paraphysis of adult necturus, same series as Fig.
 +
18, X about 120 diams.
 +
 +
 +
 +
Pig, 20 represents a small portion of the paraphysis of Fig. 17, magnified 560 diams., and shows clearly the relation of the tubules to the
 +
vessels. In the centre of the figure is a tubule, T, dividing into two
 +
branches, T^ T-. Surrounding these tubules on every side are sinusoids,
 +
si., whose flat endothelial cells are seen lying directly against the epithelial
 +
wall of the tubules with no connective tissue between them. We find
 +
here in order first a sinusoid, then a tubulcj then another sinusoid and
 +
another tubule, and finally a sinusoid. The wall of the tubules consists
 +
of a single layer of cells with large oval nuclei and very indistinct cell
 +
boundaries. The nuclei contain masses of granules arranged very irregu
 +
 +
 +
Joljn Warren
 +
 +
 +
 +
larly. There can be no question abont the glandular nature of the paraphysis, and its circulation is evidently sinusoidal.
 +
 +
The choroid plexus, Ch. Fix., Fig. 17, appears as a confused mass of
 +
 +
 +
 +
 +
Fig. 20. Small portion of adult paraphysls, same section as Fig. 17, X 560
 +
diams.
 +
 +
vessels covered by a thin layer of cells. This mass completely fills up
 +
the cavity of the fore and mid brains, and may in some cases appear in
 +
the hind brain, Fig. 23, though there seems to be considerable variation
 +
 +
 +
 +
 +
Fig. 21. Wax model of epiphysis of adult necturus. X 280 diams.
 +
 +
in the caudad development of this part of the ple.xus. Tlie two parts
 +
of the plexus overlap each other, and are also closely interlaced.
 +
 +
The epiphysis, E. is still attached to the brain by a very narrow stalk.
 +
The body overlaps the stalk somewhat behind, and then is prolonged
 +
forward as an oval flattened body above the roof of the diencephalon, and
 +
 +
 +
 +
16
 +
 +
 +
 +
PiiTajiln'sis and tlic Pineal Rogig^i in Xccturus Maculatns
 +
 +
 +
 +
its cavity seems to be divided more or less into compartments. Fig. :H
 +
represents a wax reconstrnction of an adult epiphysis seen from ^above.
 +
It is irregularly triangular in shape with a broad base and a blunt apex.
 +
Its surface is grooved more or less by vessels which lie against its Avails.
 +
Fig. 22 is the same model with the top removed. The interior is more
 +
or less subdivided by incomplete septa. At its apex there is a small cavity
 +
 +
 +
 +
 +
Fig. 22. Same as Fig. 21, with top of epiphysis removed.
 +
 +
1)0unded behind by a partial septum, then comes a large chamber, which
 +
divides into two passages running back towards the angles at the base.
 +
Between these two passages appears a comparatively solid area, interrupted, hoAvever. to some extent by small spaces, aa^IucIi communicaio Avith
 +
each other and the larger chambers. This solid area lies over tbe stalk
 +
of the organ. The sujDra commissure appears to be comparatively small,
 +
 +
 +
 +
 +
Fig. 23. Brain of adult necturus. Viewed from above. X 7 diams.
 +
 +
while the posterior is large and forms a deep groove in the roof of tlie
 +
brain. Fig. 23 is a vioAv of the brain of an adult necturus shoAving the
 +
relative positions of paraphysis and epiphysis. The tufted extremity
 +
of the diencephalic plexus can be seen in the fourth ventricle.
 +
 +
If Fig. 1, the embryo of 8-9 mm., is compared Avith Fig. 17, the adult,
 +
one sees that the paraphysal arch has been Avholly taken up in the
 +
formation of the telencephalic plexus, the plexus of the hemispheres, and
 +
 +
 +
 +
John Warren 17
 +
 +
the paraphysis. The velum and the greater part of the post-velar arch
 +
have been absorbed in the formation of the diencephalic plexus. A
 +
portion of this arch, however, persists and forms that part of the roof
 +
of the diencephalon between the diencephalic plexus and the supra commissure. The epiphysal arch has formed the epiphysis.
 +
 +
The paraphysis is a structure common to all vertebrates either in the
 +
adult or embryonic condition (Selenka, 34, Francotte, 11), but previous
 +
observations on mammals leave much to be desired. It always arises from
 +
the telencephalon cephalad to the velum transversum, and its opening
 +
is placed between and dorsad to the foramina of Munro as emphasized
 +
by Dexter (5).
 +
 +
In the cyclostomes, Ammocoetes (Kupffer, 24), and Petromyzon
 +
(Burckhardt, 3), the paraphysis appears as a small sac-like diverticulum
 +
lying ventrad and close to the enlarged distal end of the epiphysis. In
 +
elasmobranchs, Minot (28) and Locy (27) found that the paraphysis
 +
in Acanthias appears at quite a late stage as a small outgrowth from
 +
the paraphysal arch and, owing to the small size of the post-velar arch
 +
and the compression of the velum, it comes to lie immediately cephalad to
 +
the epiphysis. In ganoids, Kupffer found in Accipenser that the paraphysis appears first as a small outgrowth which later becomes a somewhat
 +
sacculated vesicle (23, Fig. 19). Hill (18) and Eycleshymer and Davis
 +
(9) studied the paraphysis in Amia. Here it begins as a simple vesicle,
 +
which increases rapidly in size and gives off diverticuli from its central
 +
cavity. In teleosts (Burckhardt, 3) the paraphysis appears late and
 +
remains in a rudimentary condition. In the dipnoi, Burckhardt (3) describes the paraphysis in Protopterus as a wide outgrowth giving off small .
 +
diverticuli.
 +
 +
In amphibia the organ becomes highly differentiated and its appearance in the adult brain is very striking. It appears as an elongated body
 +
lying above and between the hemispheres, and extending cephalad for
 +
a varjdng distance in various forms. Fig. 23. Osborn (31, PI. 4) shows
 +
a view of the brains of Siredon, Necturus, Proteus, and Siren. The
 +
paraphysis has the same general form in each of these, but it is somewhat
 +
larger in Necturus. The paraphysis of Triton and Ichthyophis (Burckhardt, 4) has the same characteristics. In the latter the paraphysis appears in sagittal section as a hammer-shaped organ extending forward
 +
above the hemispheres. (4, Fig. 1). In Rana the paraphysis has the
 +
same position as in Necturus, but is smaller. On removing the top of the
 +
skull in Necturus the paraphysis is seen lying beneath the pia surrounded
 +
by the blood-vessels which cover this part of the brain. It appears to the
 +
naked eye so vascular and also in sections so intimately related to the
 +
2
 +
 +
 +
 +
18 Paraphysis and the Pineal Pegion in Necturns Maculatus
 +
 +
choroid plexus that it is not astonishing that it was at first regarded as
 +
a portion of this plexus. According to Minot (28) the paraphysis of
 +
Eana is characterized by the character of its epithelium, its tubular structure and its apparently sinusoidal circulation. This is practically similar
 +
to the conditions found in Necturus.
 +
 +
In ^lenopoma Sorenscn (36) describes the paraph3'sis as a solid vascular mass, and in Ichthyophis Burckhardt (3) describes it as an
 +
elaborately folded structure of a glandular character. In Amblystoma,
 +
Eycleshymer (8) shows that the organ gives off tubules and has a digitated appearance. In Diemyctylus (Mrs. Gage, 13), in an embryo of
 +
10 mm. the paraphysis closely resembles that of Necturus at 18 mm.
 +
(Minot, 28, Fig. 13), and in the adult it is a long tube giving off many
 +
tubules in close relation to vessels.
 +
 +
Herrick (15) calls the paraphysis of an adult Necturus the " preparaphysis " and says it consists of an irregular central chamber with
 +
complicated diverticuli in close relation to vessels. This description
 +
corresponds closely with Fig. 17. The model of the adult paraphysis,
 +
Fig. 19, shows the complicated arrangement of the tubules, many of
 +
which anastamose with each other, and the spaces between the tubules
 +
are filled by blood-vessels.
 +
 +
Fig. 6 shows how the paraphysis at 12.5 mm. is beginning to invade a
 +
large vessel lying over it on the surface of the brain. This vessel at this
 +
point is much enlarged. Fig. 10 shows this relation much clearer and
 +
also that these vessels in relation to the paraphysis are tributaries of the
 +
internal jugular vein. Fig. 15 shows the relation of the paraphysis to
 +
these vessels at 26 mm. and that the vessels pass into the choroid plexuses
 +
both dorsad and ventrad to the organ. The little tubules can be seen growing out into the vessels. Fig. 17 shows how these relations between vessels
 +
and tubules in the adult become much more intimate, and the vessels
 +
corresponding to those in Fig. 15 are seen passing into the choroid plexuses dorsad as well as ventrad to the paraphysis. Schobel (33), who
 +
studied the circulation in the brain of certain amphibia, of which
 +
Necturus was one, shows that in the adult two large vessels pass outward
 +
just caudad to the hemispheres to empt}'^ eventually in the internal
 +
jugular vein. These vessels surround the paraphysis and anastomose
 +
with two or three large vessels running forward between the hemispheres.
 +
This is practically the same arrangement shown in the model in Fig. 10.
 +
He does not mention the paraphysis but refers to it as a large venous
 +
plexus. Eex (32) also has studied the veins in the amphibian brain,
 +
and his preparations are practically the same as Schobel's. He refers
 +
to the paraphysis as the " nodus chorioideus " and says that it is a sort
 +
 +
 +
 +
John Warren 19
 +
 +
of meeting point for the veins of the fore and mid brains. He shows
 +
beautifully in his injections how veins pass both dorsad and ventrad to
 +
the paraphj'sis to enter the plexuses, and how closely these vessels are
 +
related to the tubules of this structure. Mrs. Gage shows practically the
 +
same arrangement in Diemyctylus
 +
 +
As regards the arteries Schobel shows that they are much smaller than
 +
the veins, and describes a small vessel passing caudad to the hemispheres
 +
to pass eventually into the plexuses. The intercrescence of the tubules
 +
of the adult paraphysis and the veins is shown clearly in Fig. 30, each
 +
vessel and tubule lying back to back with no connective tissue between
 +
them. In view of all these facts it seems evident that the circulation of
 +
the paraphysis is sinusoidal. According to the above descriptions, the
 +
development of the paraphysis into a complicated, glandular organ, which
 +
is also very vascular, seems to be a striking characteristic of the amphibia.
 +
 +
In lacertilia the paraphysis of Anguis fragilis has been studied by
 +
Francotte and of Lacerta vivipara by Francotte (10, 11, 12) and
 +
Burckhardt (3). The latter shows the paraphysis in an embryo of
 +
13 mm. as a narrow tube with a slightly expanded distal extremity,
 +
much as that of ISTecturus of 15 mm. Francotte describes the paraphysis
 +
of Lacerta vivipara as a long tube giving off a mass of tubules which
 +
lies under the parietal eye, and resembles the epiphysis of birds (11, Fig.
 +
14; 12, Fig. 24). In Anguis (10, Figs. 15 and 19) the paraphysis forms
 +
a long narrow sack, with somewhat convoluted walls, which curves back
 +
over the post-velar arch to end in close relation to the parietal eye.
 +
 +
The conditions in the lizard are essentially the same (10, Fig, 31).
 +
In Phrynosoma coronata Sorensen (35, Fig. 2) describes the paraphysis
 +
as a long, narrow tube, immediately cephalad to the epiphysis.
 +
 +
In the ophidia Leydig (26, Fig. 6) shows the paraphysis of an embryo
 +
of Vivipara urcini near birth as a large, wide tube with no convolutions
 +
or diverticuli and practically the same conditons in a young " Eingelnatter" and Tropidonatus natrix (26, Figs. 5 and 2).
 +
 +
Among the chelonia Voelzkow (39) has described the paraphysis in
 +
Chelone imbricata as at first a wide tube much convoluted, which later
 +
decreases somewhat in size. (Figs. 21 and 22.) Its distal end inclines
 +
caudad close to the epiphysis. In Chelone mydas Humphrey (20, Fig. 7,
 +
PI. II) shows the paraphysis as a long tubular structure, giving off small
 +
tubules, and in an embryo of Chelydra it appears as a large, wide sack,
 +
from which tubules arise. It is in closer relation to the epiphysis than
 +
in Chelone mydas or imbricata.
 +
 +
In Cistudo Herrick shows a model of Sorensen (15, Fig. 5, PI. VI)
 +
of the paraphysis, which is a wide tube with convoluted walls and tubules.
 +
 +
 +
 +
20 Paraphysis and the Pineal Region in Necturus Maculatus
 +
 +
In the crocodilia Voelzkow (39) has described the paraphysis of
 +
Crocodilus madagascarensis grand and Caiman niger spix. In the former
 +
the paraphysis is at first a wide tube which becomes convoluted and much
 +
longer and narrower. In the latter the paraphysis forms a larger tube
 +
and the convolutions and tubules are more complicated. In both cases
 +
the organ reaches its greatest development in embryonic life and retrogrades later, though more so in the crocodile. He was unable, however,
 +
to follow the development in the caiman as far as in the crocodile.
 +
Owing to the thickenings in the brain wall the organ is crowded somewhat caudad against the post-velar arch.
 +
 +
In birds the paraphysis is relatively rudimentary. Burckhardt (3)
 +
shows the paraphysis in an embryo of the crow as a small diverticulum
 +
not unlike that of Petromyzon. Dexter (5) worked out in detail the
 +
development of the organ in the common fowl, and showed that it appeared at first as a small diverticulum. The walls become much thickened and in a chicken of 10 days it is a small, oval structure, about 150 fx
 +
in its greatest diameter, with very thick walls (5, Fig. 5). Selenka (34)
 +
has described the paraphysis in the oppossum, but as far as I am aware
 +
little is known of the development of the paraphysis in mammals, though
 +
Francotte (11) has observed it in human embryo of twelve weeks.
 +
 +
From the cyclostomes to the amphibia the paraphysis shows a steadily
 +
progressive development, and the various forms through which it passes,
 +
from the simple diverticulum of Petromyzon to the elaborate gland of
 +
the urodela, are illustrated in a general way by the stages of its development in Necturus. In the vertebrates above the amphibia the paraphysis
 +
retrogrades and practically retraces its steps through the reptilia and
 +
birds to mammals, reaching in the chick essentially the same form in
 +
which it started in Petromyzon. Its development, therefore, may be
 +
indicated by a curve, which ascends steadily from the cyclostomes, reaches
 +
its height in urodela, and descends through the reptilia and birds to
 +
mammals.
 +
 +
The epiphysis is present in nearly all vertebrates. It is stated to be
 +
absent in the alligator (Sorensen, 36 and 37), and in the caiman and
 +
crocodile (Voelzkow, 39) and in Torpedo (d'Erchia, 7).
 +
 +
The epiphysis of Necturus as compared with the paraphysis is relatively
 +
poorly developed and in this respect resembles the epiphysis of other
 +
urodela (Mrs. Gage, 13). In Diemyctylus Mrs. Gage found that the
 +
epiphysis was entirely cut off from the brain and that its cavity was
 +
nearly obliterated. In Ichthyophis (Burckhardt, 1 and 4) the epiphysis
 +
is a small, pear-shaped organ attached to the brain by a narrow solid
 +
stalk. Herrick (15) in Menopoma, describes the epiphysis as an irreg
 +
 +
 +
John Warren 21
 +
 +
ular number of vesicles attached to the brain by a narrow opening. According to Kingsbury (21) the structure in Necturus consists of an
 +
aggregation of closed vesicles, forming an oval, flattened body, and there
 +
is no connection with the brain. The cavity of the epiphysis communicates through its stalk with the cavity of the diencephalon up to 15 mm.,
 +
when the cavity in the stalk becomes obliterated. The stalk persists and
 +
was present in all the adult brains which I examined, but in some cases
 +
it was so small that it could easily be overlooked. The reconstruction
 +
of an adult epiphysis. Fig. 22, shows that the cavity of the organ forms
 +
a large chamber subdivided to a certain extent by incomplete septa. A
 +
much more solid area is seen towards the caudal extremity, which is
 +
placed just over the stalk. The same characteristics I have observed in
 +
another model made from a different brain. One gets the idea that the
 +
epiphysis consists of a series of vesicles in studying sagittal sections a
 +
little to one side of the median line, as for instance in Fig. 17, where the
 +
epiphysis was displaced a little to one side.
 +
 +
. There has been such a vast amount written on the origin of the
 +
epiphysis and the pineal or parietal eye and their homologies that it seems
 +
superfluous to add anything more here. In a very general way, however,
 +
there seems to be some sort of proportion in the relative development of
 +
the paraphysis, epiphysis, and the parietal eye. In urodela where there
 +
is no parietal eye and a small epiphysis, the paraphysis reaches its highest
 +
degree of development. In those forms where the paraphysis is rudimentary or relatively slightly developed the parietal eye is present or
 +
else the epiphysis is relatively highly developed. Compare, for example,
 +
the figures of Burckhardt (3) of Petromyzon, Minot (28) of Acanthias,
 +
Burckhardt (3) of Trout, Leydig (26) and Voelzkow (38) of reptilia,
 +
and Dexter (5) of the fowl. Rana, however, seems to be a marked exception to this statement, as there the paraphysis, epiphysis, and pineal
 +
eye are all present and well developed, and the same may be said for
 +
Lacerta (Francotte, 10 and 11) and Sphenodon (Dendy, 6). As the
 +
paraphysis and epiphysis are glandular structures they have probably
 +
some sort of compensatory function and where one is highly developed
 +
the other is relatively rudimentary or even absent. Compare in this
 +
respect also Torpedo with Acanthias and the crocodile and alligator with
 +
the chick.
 +
 +
As a rule the stalk of the epiphysis is placed immediately caudad to the
 +
supra commissure, in all cases I believe, except in the toad, where there
 +
is a distinct interval between it and this commissure (Sorenson, 36).
 +
In Necturus there is an interval in the roof of the brain between the stalk
 +
and the posterior commissure. This portion of the roof of the brain was
 +
 +
 +
 +
22 Paraphysis and the Pineal Eegion in Necturus Macnlatus
 +
 +
described by Kupffer (23) as the " schaltstlick," and according to him it
 +
is best developed in amphibia. Burckhardt (3) maintains that it occurs
 +
in all vertebrates from Pctromyzon np, but according to Kupffer it is
 +
absent in Accipenser (23, Fig. 19), and it is also wanting in Acanthias
 +
(Minot, 28, Pig. 10) and in the fowl (Dexter, 5, Fig. 9).
 +
 +
The velum transversum is probably characteristic of all vertebrates.
 +
Minot (28). In Petromyzon the velum appears as a small transverse
 +
fold, and the post-velar arch is well marked. The plexus development
 +
is, however, very slight. In elasmobranchs the velum of Acanthias
 +
forms a long, narrow, transverse fold, and the post-velar arch is so small
 +
that the origin of the velum seems to be close to the supra commissure.
 +
The caudal layer of the velum is distinctly thinner than the cephalic
 +
(Minot, 28, Fig. 6). This is also seen in Torpedo (d'Erchia, 7, Fig.
 +
12), and in Necturus, Fig. 6. The velum later on has the character of
 +
a choroid plexus, but the plexus of the hemispheres is very rudimentary
 +
(Minot, 28). In Notidanus Burckhardt (3) shows a long, narrow velum,
 +
a short post-velar arch, and a small telencephalic plexus. The plexus
 +
of the hemispheres, however, is absent.
 +
 +
In Accipenser (Kupffer, 23, Fig. 19) the velum is long, well developed
 +
and folded to a certain extent, and the post-velar arch is quite extensive.
 +
In ganoids (Studnicka, 38) the membranous roof of the brain serves as
 +
the tela choroidea of higher types. In this class of vertebrates according
 +
to Burckhardt (3) the plexus of the hemispheres is lacking, but the
 +
telencephalic plexus is well developed, and in teleosts the former is also
 +
wanting, but the latter present in a reduced form.
 +
 +
In amphibia all the plexuses are highly developed, and in J^ecturus
 +
they are of marked extent (Kingsbury, 21). The velum in Necturus
 +
appears at first as a transverse fold in the roof of the brain separating
 +
the diencephalon from the telencephalon. This fold develops at first
 +
ventrad and then caudad through the mid brain as far as the hind brain.
 +
This great growth of the velum forms the diencephalic plexus. The
 +
post-velar arch, which at first is wide and well marked, is practically
 +
absorbed by the overgrowth of the velum, and a small portion only
 +
persists in the roof of the diencephalon between the origin of the diencephalic plexus and the supra commissure. Fig. 17. The telencephalic
 +
plexus develops from the paraphysal arch immediately cephalad to the
 +
paraphysis, the opening of which therefore is surrounded by these two
 +
plexuses. They fill up the cavity of the third ventricle and mid brain,
 +
and the diencephalic plexus may appear in the hind brain (Osl>orn, 29).
 +
This seems to vary in different cases, and in the majority of brains which
 +
I was able to examine the extremity of this plexus did not actually extend
 +
 +
 +
 +
John Warren 23
 +
 +
into the hind brain. In- Fig. 33, however, this extremity appears as a
 +
marked tuft in the fourth ventricle. The plexuses of the hemispheres
 +
arise on either side from the origin of the telencephalie plexus and pass
 +
into the lateral ventricles, extending nearly to their cephalic extremities.
 +
 +
In Lacerta vivipara (Francotte, 12, Fig. 24) the post- velar arch has
 +
been much com})ressed from before backward so as to form a deep narrow
 +
angle. At the apex of the angle the folds of the diencephalic plexus are
 +
seen. The velum is smooth and apparently is not included in the
 +
formation of the plexus. In Anguis fragilis (Francotte, 10, Figs. 19
 +
and 15), the post-velar arch does not seem to be so much compressed,
 +
and the plexus formation somewhat greater. As he says, however, the
 +
development of those parts in Lacerta is practically the same as in Anguis.
 +
According to Burckhardt the telencephalie plexus is much reduced
 +
in size, consisting merely of small folds, but the plexus of the hemispheres is well developed (Burckhardt, 3).
 +
 +
In the turtles Humphrey (20) found that the velum of Chelydra is
 +
but slightly developed, and no diencephalic plexus is formed. All the
 +
other plexuses are telencephalie in origin.
 +
 +
Herrick (15, Fig. 5, PI. VI), shows in Cistudo a well-developed telencephalie plexus and a diencephalic plexus represented by many folds in
 +
the caudal layer of the velum and the post-velar arch. In Chelone imbricata Yoelzkow (39, Figs. 19 and 22) shows at first a well marked
 +
velum and a wide post-velar arch. In later stages the velum and practically all the arch are thrown into folds to form the diencephalic plexus.
 +
The telencephalie plexus is also well developed. In the serpents much
 +
the same arrangement can be seen. The velum (Leydig, 26, Figs.
 +
2, 5, and 6) forms a prominent fold, and it and the post-velar arch
 +
form a very vascular plexus. In the crocodilia (Voelzkow, 39, Figs.
 +
7, 11, 13, 15), the velum and the post-velar arch are at first well
 +
marked, but the parts later become so compressed from before backward that the arch forms a deep acute angle in the depths of which plexus
 +
foldings are seen. The caudal layers of the velum, however, takes no part
 +
in the plexus formation. In birds, Dexter (5) found that the velum of
 +
the fowl is small and the post-velar arch broad at first. This becomes
 +
compressed so as to form an acute angle much as in the crocodilia. The
 +
cephalic limb of this angle and all the velum is converted into the choroid
 +
plexus. In birds the plexus of the hemispheres is very well developed,
 +
but the telencephalie plexus is practically absent.
 +
 +
In fishes the plexus development is quite simple, in many cases being
 +
merely the thin membranous roof of the third ventricle; in others, however, this is much folded and vascular (Sorensen, 35). In certain forms
 +
 +
 +
 +
24 Paraphysis and tfio Pineal Eegion in Necturus Maculatus
 +
 +
there is a telenccphalic plexus, but the plexus of the hemispheres is
 +
absent or rudimentary (Burckhardt, 3). In amphibia there is a great
 +
overgrowth of all the plexuses, especially of the diencephalic plexus,
 +
which here reaches its highest development. In reptilia the plexus of the
 +
hemispheres is well developed, but the telencephalic plexus is reduced
 +
in size (Burckhardt, 3), and the diencephalic much more so. In birds
 +
the plexus of the hemispheres is highly developed, the telencephalic
 +
plexus practically absent, and the diencephalic plexus, while very similar
 +
to that of reptilia, approaches nearer to the tela choroidea of higher
 +
mammalia.
 +
 +
Osborn first named the supra-commissure and worked out its homologies. According to him (30) the urodela are distinguished from the
 +
anura by the frequent extensive development of this commissure, which
 +
is large in Amphiuma, smaller in Necturus, and much reduced in Eana.
 +
It appears in Necturus a little later than the posterior commissure, as
 +
is usual in most cases, as far as I am aware, except in Ammocoetes, where
 +
it appears shortly before the posterior commissure (Kupffer, 24, Fig. 5).
 +
It is found in all the chief types of vertebrates, and is usually smaller
 +
than the posterior (Minot, 28). It is developed from the diencephalon,
 +
while the posterior belongs to the cephalic limit of the mid brain.
 +
 +
Conclusions.
 +
 +
1. The paraphysis appears first in an embryo of 12 mm. It is developed from the telencephalon immediately cephalad to the velum transversum as a small diverticulum, which becomes eventually a complicated
 +
gland with anastomosing tubules. The gland is very vascular, and has a
 +
sinusoidal circulation.
 +
 +
2. The epiphysis appears first in an embryo of 9-10 mm., and is developed from the diencephalon. It is always attached to the brain by a
 +
small solid stalk, and the cavity is partially subdivided by incomplete
 +
septa.
 +
 +
3. The velum transversum grows at first ventrad and then caudad as
 +
far as the hind brain, forming in this way the diencephalic portion of
 +
the choroid plexus. The post-velar arch, which is at first quite extensive,
 +
is almost entirely absorbed in this extensive growth of the v.elum.
 +
 +
4. The telencephalic plexus arises from the roof of the telencephalon,
 +
and fills up the depths of the cavity of the third ventricle. The opening
 +
of the paraphysis is surrounded by these two plexuses.
 +
 +
5. The plexus of the hemispheres arises at a right angle from the
 +
telencephalic plexiis just cephalad and ventrad to the opening of the
 +
paraphysis.
 +
 +
 +
 +
John Warren 25
 +
 +
6. The supra-commissure appears first at 16-17 mm. It lies immediately cephalad to the stalk of the epiphysis and is comparatively small.
 +
 +
7. The posterior commissure appears first at 15 mm., and there is a
 +
marked interval in the roof of the diencephalon between it and the
 +
epiphysis.
 +
 +
I wish in conclusion to express my acknowledgments to Prof. Minot
 +
for his kind advise and interest in the preparation of this article.
 +
 +
BIBLIOGRAPHY.
 +
 +
The following are the principal articles consulted, but of course do not
 +
form a complete bibliography of this subject:
 +
 +
1. BuRCKHAKDT, R. — Die Zirbel von Ichthyophis Glutinosus und Protopterus
 +
 +
Annectens. Anat. Anz., Bd. VI.
 +
 +
2. Die Homologien des Zwischenhirndaches bei Reptilien und Vogeln.
 +
 +
Anat. Anz., Bd. IX, 320-324.
 +
 +
3. Der Bauplan des Wirbeltiergehirns. Morpholog. Arbeiten, IV
 +
 +
 +
 +
Bd., 2 Heft, 131.
 +
 +
4. Untersuchungen am Gehirn und Geruchsorgan von Triton und
 +
 +
Ichthyophis. Zeitschr. f. Wiss. Zoologie, Bd. 52.
 +
 +
5. Dexter, F. — The Development of the Paraphysis in the Common Fowl.
 +
 +
American Journ. Anat., Vol. II, No. 1, 13-24.
 +
 +
6. Dendy, a. — On the Development of the Pineal Eye and Adjacent Organs
 +
 +
in Sphenodon (Hatteria). Quart. Journal Micros. Soc, Vol. 42, 111.
 +
 +
7. D'Ebchia, F. — Contributo alio studio della volta del cervello intermedio
 +
 +
e della regione parafisaria in embrioni di Pesci e di Mammiferi.
 +
Monitore Zoologico, VII, 118 e 201.
 +
 +
8. Eycleshymer, a. C. — Paraphysis and Epiphysis in Amblystoma. Anat.
 +
 +
Anz., Bd. VII.
 +
 +
9. Eycleshymer, A. C, and Davis, B. M. — The Early Development of the
 +
 +
Paraphysis and Epiphysis in Amia. Journal of Comp. Neurology,
 +
Vol. 7.
 +
 +
10. Francotte, p. — Recherches sur le developpement de L'epiphyse. Arch.
 +
 +
de biologie, T. VIII.
 +
 +
11. Note sur I'ceil parietal, l'epiphyse, la paraphyse et les plexus
 +
 +
choroides du troisieme Ventricule. Bull, de I'acad, royale, etc., d.
 +
Belg., 3 Serie, T. 27.
 +
 +
12. Contribution a I'tHude de I'oeil parietal, de l'epiphyse chez les
 +
 +
Lacertiliens.
 +
 +
13. Gage, S. P. — The Brain of Diemyctylus Viridescens. Wilder Quart. Cent.
 +
 +
Book, 1898.
 +
 +
14. Gaupp, E. — Zirbel, Parietalorgan und Paraphysis. Ergebn. Anat. Entwick.
 +
Ges., VII, 208-285.
 +
 +
15. Hebbick, C. L. — Topography and Histology of the Brain of certain Rep
 +
tiles. Journ. of Comp. Neurology, Vol. I, 37; Vol. Ill, 77-104, 119-138.
 +
 +
16. Topography and Histology of certain Ganoid Fishes. Journ. of
 +
 +
Comp. Neurology, Vol. I, 162.
 +
 +
 +
 +
26 Paraphysis and the Pineal Kegion in Nectun;s Macnlatus
 +
 +
17. Hkkuick, C. L. — Embryological Notes on the Brain of a Snake. Journ.
 +
 +
Neurology, Vol. I, lGO-176.
 +
IS. Hill, C. L. — The Epiphysis in Teleosts and Amia. Journ. of Morphology.
 +
 +
Vol. IX, 237-268.
 +
 +
19. His, W. — Zur allgemeinen Morphologie des Gehirns. His. Archiv, 1892,
 +
 +
346-383.
 +
 +
20. Humphrey, O. D. — On the Brain of the Snapping Turtle. Journ. of Comp.
 +
 +
Neurology, Vol. IV, 73-108.
 +
 +
21. Kingsbury, B. F. — The Brain of Necturus Maculatus. Journ. of Comp.
 +
 +
Neurology, Vol. V.
 +
 +
22. The Encephalic Evaginations in Ganoids. Journ. of Comp. Neurology, Vol. VII.
 +
 +
23. KxiPFFER, C. V. — Studien zur vergleichenden Entwicklungsgeschichte des
 +
 +
Kopfes der Kranioten. Hefte I.
 +
 +
24. Derselbe. Hefte II.
 +
 +
25. Lewis, F. T. — The Question of Sinusoids. Anat. Anx., Bd. XXV, No. 11.
 +
 +
26. Leydig, F. — Zirbel und Jacobsonsche Organe einiger Reptilien. Archiv f.
 +
 +
Mikrosk. Anatomie, Bd. 50.
 +
 +
27. LocY, W. A. — Contribution to the Structure of the Vertebrate Head.
 +
 +
Journ. of Morphology, XI.
 +
 +
28. MiNOT, C. S. — On the Morphology of the Pineal Region, based on its
 +
 +
Development In Acanthias. American Journ. of Anatomy, Vol. I,
 +
No. 1, 81-98.
 +
 +
29. On a Hitherto Unrecognized Form of Blood Circulation without
 +
 +
Capillaries in Organs of Vertebrates. Pro. Boston Soc. Nat. Hist.,
 +
Vol. 29, No. 10, S. 185-215.
 +
 +
30. OsBORN, H. F. — Preliminary Observations on the Brain of Menopoma.
 +
 +
Proceed. Phil. Acad., 1884.
 +
 +
31. Contribution to the Internal Structure of the Amphibian Brain.
 +
 +
Journ. of Morphology, Vol. II, 51-86.
 +
 +
32. Rex, H. — Beitrage zur Morphologie der Hirnvenen der Amphibien. Morph.
 +
 +
Jahrb., XIX, 295-311.
 +
 +
33. ScHOBEL, Jos. — Ueber die Blutgefasse des Cerebrospinalen Nervensystems
 +
 +
der Urodelen. Archiv f. Wissen. Mikros., Bd. XX, 87-91.
 +
 +
34. Selenka, E. — Das Stirnorgan der Wirbelthiere. Biolog. Centralbl., Bd. X,
 +
 +
323-326.
 +
 +
35. Sorensen, a. D. — The Roof of the Diencephalon. Journ. of Comp. Neu
 +
rology, III, 50-53.
 +
 +
36. Comparative Study of the Epiphysis and the Roof of the Diencephalon. Journal Comp. Neurology, IV, 153-170.
 +
 +
37. Continuation of above. Vol. IV, 153-170.
 +
 +
38. Studenicka, F. Cii. — Beitrage zur Anatomie und Entwicklungsgeschichte
 +
 +
des Vorderhirns der Cranioten.
 +
 +
39. VoELZKOw, A. — Epiphysis und Paraphysis bei Krokodilien und Schild
 +
krot>en. Abhand. der SenchenbiXrgischen Naturforschenden Gesellschaft, Bd. XXVII, Heft. II.
 +
 +
 +
 +
John Warren
 +
 +
 +
 +
27
 +
 +
 +
 +
ABBREVIATIONS.
 +
 +
 +
 +
A. C. — Anterior commissure. L. Y.
 +
Ch. Fix. — Choroid plexus. M. B
 +
Dien. — Diencephalon. 0. C
 +
D. Flex. — Diencephalic plexus. Tel. Plx.
 +
i?.— Epiphysis. Tel.
 +
Ep. A. — Epiphysal arch. F. C
 +
F. B. — Fore-brain. P. T- A. —
 +
 +
F. M. — Foramen of Munro. ■ P. A.
 +
H. — Hemisphere. F.
 +
 +
H-JB.— Hind brain. S.C.
 +
 +
Hyp. — Hypophysis. Si.
 +
 +
I. J. V. — Internal jugular vein. T.
 +
 +
L. Fix. — Choroid plexus of lateral Tes.
 +
 +
ventricle. ^'
 +
 +
 +
 +
Lateral ventricle.
 +
Mid-brain.
 +
Optic commissure.
 +
-Telencephalic plexus.
 +
-Telencephalon.
 +
-Posterior commissure.
 +
Post-velar arch.
 +
Paraphysal arch.
 +
Paraphysis.
 +
-Superior commissure.
 +
Sinusoid.
 +
•Tubule.
 +
■Vessel.
 +
-Velum transversum.
 +
 +
 +
 +
THE DEVELOPMENT OF THE THYMUS.
 +
 +
BY
 +
 +
E. T. BELL, B. S., M. D.
 +
 +
Instructor in Anatomy, University of Missouri.
 +
 +
With 3 Plates and 5 Text Figures.
 +
 +
This paper is intended mainly as a contribution to our knowledge of
 +
the histogenesis of the thymus in mammals. Special attention is given
 +
to the origin and development of the corpuscles of Hassall, since their
 +
mode of formation has never been satisfactorily described in mammals
 +
and their significance in all forms is in dispute. An attempt is also
 +
made to show in detail the changes that occur during the transformation
 +
of the thymus from the epithelial to the lymphoid condition.
 +
 +
This work was begun at the suggestion of Dr. D. D. Lewis at the
 +
University of Chicago. The greater part of it has been done at the
 +
University of Missouri. Special acknowledgments are due Dr. C. M.
 +
Jackson for valuable criticism and suggestions. T wish also to thank
 +
Mr. Charles H. Miller of the University of Chicago for his kindness in
 +
sending me material.
 +
 +
Material and Methods.
 +
 +
As material for the greater part of my work, I. have used pig embryos
 +
from 8 mm. to full term (26 cm. to 30 cm.). These are especially suitable for such work since they may be procured in abundance from the
 +
large packing houses at almost any stage of development. For special
 +
purposes I have studied a few specimens from human foetuses, and from
 +
the cat, rat, and guinea pig. The smaller pigs used (8 mm. to 27 mm.')
 +
belong to the collection in the anatomical laboratory at the University of
 +
Missouri. These were stained in bulk with alum-cochineal and mounted
 +
in serial sections. In the later stages, which were prepared specially for
 +
this work, tbe ventral half of the cervical and anterior thoracic regions
 +
was usually out out and embedded from pigs from 3 cm. to 8 cm. On
 +
specimens from 8 cm. to 30 cm.. I dissected out the thymus and used sucb
 +
parts as were desired.
 +
 +
^ The crown-rump measurement is used in all cases.
 +
 +
AMERICAN .TOCTRNAL OF ANATOMY. VOI,. V.
 +
 +
 +
 +
30 Tlie Development of the Thymus
 +
 +
All pig material was fixed in Zenker's fluid, embedded in paraffin, and
 +
mounted in serial sections from 3 ju, to 10 /x thick. Except those of the
 +
young stages (8 mm. to 27 mm.) the sections were stained on the slide.
 +
Most of them were stained with hsematoxylin or iron-hgematoxylin and
 +
counterstained with Congo red. For special purposes many other stains
 +
were used.
 +
 +
To demonstrate the delicate protoplasmic threads of the syncytium
 +
during the later stages of the lymphoid transformation, I stained by the
 +
iron-haematoxylin method but omitted the final decolorization in ironalum. Protoplasm is stained deep black; nuclear structure is poorly
 +
shown, but the finest cytoplasmic processes may be seen.
 +
 +
For the demonstration of connective tissue fibrillae in the syncytium,
 +
I found the method recommended by Jackson (13, S. 39) most satisfactory.
 +
 +
Text Fig. 2.
 +
 +
Text Figure 1. Cranial view of third gill pouch (thymic anlage) ; X 33;
 +
pig embryo, 11 mm.; ec, ectoderm; I, lumen; nt, nodulus thymicus; ph, connection to pharynx; s p, sinus prsecervicalis.
 +
 +
Text Figure 2. Ventral view of thymic anlage; X 33; pig embryo, 15 mm.;
 +
ec, ectoderm; I, lumen; n t, nodulus thymicus; ph, connection to pharynx;
 +
s p, sinus prsecervicalis.
 +
 +
To determine the relation of the blood-vessels to the corpuscles of
 +
Hassall, I put a young kitten under deep anesthesia and injected a large
 +
quantity of a strong aqueous solution of Prussian blue into the aorta
 +
through the common carotid artery. The heart continues to beat even
 +
after an amount of fluid twice as great as the total volume of the blood
 +
has been injected. An injection made in this way is under a slightly
 +
increased blood pressure and easily reaches the finest capillaries. There
 +
is therefore a thorough injection with little danger of rupturing delicate
 +
blood-vessels.
 +
 +
Organogenesis.
 +
My observations on this phase of development are not sufficiently complete to warrant a full discussion. A brief survey may however prepare
 +
the way for a better understanding of the histogenesis. The text figures
 +
show the shape in outline of the third gill pouch and thymic anlage
 +
 +
 +
 +
 +
E. T. Bell 31
 +
 +
from 11 mm. to 27 mm. . They are graphic reconstructions. Since this
 +
pouch is nearly all converted into thymus it may be regarded as the
 +
thymic anlage from a very early stage.
 +
 +
At 11 mm. (Text Figure 1), the pouch is a hollow epithelial tube directed from without ventrally and mesially. The lumen (l) is large
 +
and communicates freely with the pharynx. On the dorso-lateral aspect
 +
of the pouch is a solid epithelial mass (n t) distinctly different in structure from the rest of the pouch. This is the nodulus thymicus
 +
(Kastschenko, 14) and will be referred to by that term. This structure
 +
has been described by Stieda (26), Prenant ' (22), and others as the
 +
anlage of the carotid gland.
 +
 +
It was evidently mistaken by Minot ' in a 12-mm. pig for the anlage of
 +
the entire thymus. It may be seen as early as the 8 mm. stage budding
 +
off from the cranio-lateral aspect of the pouch. Immediately behind the
 +
nodulus thymicus, but not connected to it at this stage is the inner blind
 +
extremity of the sinus prsecervicalis (s p). These become fused at
 +
12 mm. or 13 mm.
 +
 +
At 15 mm. (Text Figure 2) the thymic anlage is more elongated. It
 +
now projects ventrally and medianwards, its free end lying immediately
 +
caudad to the median thyroid anlage and just craniad to the pericardium.
 +
Its lumen (Z) is still in communication with the pharynx. The sinus
 +
prsecervicalis {s p) is drawn out, its lumen being smaller and longer.
 +
It is now fused to the outer two-thirds of the posterior aspect of the
 +
nodulus thymicus (n t).
 +
 +
At 18 mm. (Text Figure 3) the anlage is growing rapidly in a caudal
 +
direction and just entering the thoracic cavity. It is connected to the
 +
pharynx by a delicate epithelial cord. There is still a lumen in its caudal
 +
part. The sinus praecervicalis has lost its connection to the nodulus
 +
thymicus." The outer part of its lumen has disappeared and it seems
 +
about to lose its connection with the ectoderm.
 +
 +
At 20 mm. (Text Figure 4) the thymus extends well into the thoracic
 +
cavity. Its thoracic segment (t h) has increased considerably in size and
 +
is united to the gland of the opposite side.
 +
 +
The nodulus thymicus still forms the greater part of the head. The
 +
anlage has no connection with the pharynx or the epidermis. There is
 +
 +
"Prenant is said to have since abandoned this idea and accepted Kastschenko's view. (v. Ebner in Kolliker's Gewebelehre des Menschen. Aufl.
 +
6, Bd. 3, 1, S. 340.)
 +
 +
^Laboratory Text of Embryology, p. 191; also p. 209 and Fig. 124.
 +
 +
* On the opposite side in this specimen, .these structures were fused over a
 +
very small area.
 +
 +
 +
 +
32
 +
 +
 +
 +
The Development of the Thymus
 +
 +
 +
 +
now an elongated mass of thymic tissue extending upwards behind the
 +
nodiilus thymicus, and fused with it. Its upper pointed extremity curves
 +
outwards around the hypoglossal nerve. This is the " thymus superficialis " (t s) of Kastschenko and is regarded by him as being formed
 +
from the sinus praecervicalis. Kastschenko describes this elongated
 +
portion as being always separate from the rest of the head, being connected only by connective tissue. In my preparations it is clearly continuous with the rest of the anlage, and seems to have formed by growing
 +
out from it. The presence of a lumen in its lower end favors Kast
 +
 +
 +
.ec
 +
 +
 +
 +
 +
 +
Text Fig. 3.
 +
 +
 +
 +
Text Fig. 4.
 +
 +
 +
 +
Text Figure 3. Ventral view of thymic anlage; X 33; pig embryo, 18 mm.;
 +
ec, ectoderm; I, lumen; nt, nodulus thymicus; ph, connection to pharynx;
 +
s p, sinus praecervicalis.
 +
 +
Text Figure 4. Ventral view of thymic anlage; X 33; pig embryo, 20 mm.;
 +
f, area fused with gland of opposite side; I, lumen; nt, nodulus thymicus;
 +
th, thoracic segment; t s, thymus superficialis.
 +
 +
schenko's view, for there is no lumen in the head at 18 mm. in my preparations. On the other hand the separation from the head at the 18 mm.
 +
stage favors the idea that the sinus prsecervicalis degenerates. I have not
 +
studied a sufficient number of specimens at this transition stage to
 +
enable me to decide this point, though I believe the ectoderm takes no
 +
part in the formation of the thymic anlage. Kastschenko's results are
 +
opposed by nearly all other students of this problem, but his work should
 +
not be discarded before the development of the thymus superficialis in
 +
the pig has been accurately determined.
 +
 +
At 27 mm. (Text Figure 5) the thymus is much longer and extends
 +
 +
 +
 +
E. T. Bell
 +
 +
 +
 +
33
 +
 +
 +
 +
well down into the thoracic cavity in relation to the base of the heart
 +
where it has fused with the gland of the opposite side. Buds are now
 +
beginning to form through the greater part of its extent. Traces of the
 +
original lumen (I) may still be seen in several places. The "thymus
 +
superficialis " is bent around the twelfth nerve. A delicate cord of thymic
 +
 +
 +
 +
 +
Text Fig. 5.
 +
 +
Text Figxjre 5. Ventral view of thymic anlage; X 33; pig embryo, 27 mm.;
 +
/. area fused with gland of opposite side; 1, lumen; nt, nodulus thymicus;
 +
th, thoracic segment; t s, thymus superficialis.
 +
 +
tissue fused with the l)ack of the nodulus thymicus connects the thymus
 +
superficialis to the rest of the head.
 +
 +
From about 3 cm. until toward the end of fcetal life the thymus shows
 +
the two constrictions, described by Prenant (22) (for the sheep) as the
 +
intermediary and the cervico-thoracic cords. These cords connect three
 +
3
 +
 +
 +
 +
3-i Tlic Development of the Tliynius
 +
 +
enlargements which we may call the head, the luid-t-ervical segment, and
 +
the thoracic segment. I will consider each part separately. The thoracic
 +
segment develops rapidly, spreading out ahove and in front of the heart.
 +
The glands of the two sides fuse completely in this region. The lymphoid
 +
transformation is noticeable at 3.5 cm. and well advanced at 4.5 cm. The
 +
medulla begins to form at 8 cm. The cervico-thoracic cord is at first
 +
very narrow but soon thickens and joins the cord of the opposite side.
 +
At full term they form a sharp constriction, 3 mm. to 4 mm. wide and
 +
5 mm. to 6 mm. long, situated at the superior aperture of the thorax,
 +
and connecting the mid-cervical and thoracic segments. The histological
 +
changes take place here later than in the enlargements.
 +
 +
The mid-cervical segment develops like the thoracic segment but somewhat more slowly. Budding, lymphoid transformation, and formation
 +
of the medulla all begin here a little later than in the head and thoracic
 +
segment. Its caudal end is slightly in advance of its cranial end. The
 +
intermediary cord is well marked at 4 cm. It soon becomes very attenuated, having at 6 cm. in many places a diameter of only 15 fx. Prenant
 +
suggests that this drawing out of the gland is caused by the rapid growth
 +
of the neck. Later it increases in size and at full term is noticeable only
 +
as a very slight constriction between the head and the mid-cervical
 +
segment. The histological changes are much later here than in other
 +
parts of the gland.
 +
 +
The greater part of the head in the early stages is formed by the
 +
nodulus thymicus. This body grows slowly, attaining a diameter in crosssection of .5 mm. at 8 cm. Its cross-sectional area at 8 cm. is about onethird that of the rest of the head of the thymus. At this stage the nodulus
 +
thymicus is a rounded body lying on the inner aspect of the head in relation to the carotid artery. A small area of its outer surface is fused
 +
with the lymphoid tissue of the thymus. Its histological structure has
 +
been fully described by Prenant (22). From the earliest stages, it
 +
consists of cords of epithelial cells separated by blood capillaries. At
 +
8 cm. the thymus superficialis (Kastschenko) is a large body lying craniad
 +
and dorsal to the rest of the head but connected to it at its caudal
 +
extremity.
 +
 +
I have no observations on the head of the thymus between 8 cm. and
 +
full term, having overlooked it in collecting my material. In a full term
 +
pig (30 cm.), the cervical part of the thymus is in two distinct parts.
 +
The postero-ventral part, representing part of the head, the intermediary
 +
cord, and the mid-cervical segment, is about 3 cm. long and 1 cm. wide.
 +
It extends from the upper border of the thyroid cartilage to the thorax,
 +
and with the corresponding part of the opposite gland encloses the
 +
 +
 +
 +
E. T. Bell 35
 +
 +
trachea, thyroid, and lower ]iait of the larynx. A slight narrowing at
 +
the junction of the upper and middle thirds indicates the position of the
 +
intermediary cord. The antero-dorsal part^ the thymus superficialis, is
 +
rounded in cross-section, tapei-rrfg- to a point behind. Its anterior end is
 +
about 7 mm. in diameter and loops around the twelfth nerve as in the
 +
earliest stages. A lobule hangs over ventral to the nerve, a thin cord
 +
being dorsal to it. The posterior end of the thymus superficialis extends
 +
to the cricoid cartilage dorsal to the postero-ventral part of the gland.
 +
It is united to this part of the gland by a very delicate band of thymic
 +
tissue. I did not find the nodulus thymicus at full term. It has either
 +
moved away from the head or degenerated.
 +
 +
The duct of the thymus is the lumen of the third gill pouch. A
 +
glance at the Text Figures will show its development. It is broken up
 +
into segments and finally obliterated. I could find no traces of it at
 +
3.7 cm. or later. On the theory of the exclusively endodermal origin of
 +
the thymus. I cannot explain the absence of a lumen in the head at
 +
IS mm. and its presence at 20 mm. and 27 mm. unless it be due to
 +
individual variation in different specimens.
 +
 +
It appears from the foregoing that in the pig the exclusively endodermal origin of the thymus from the third gill [loueh is probable, but
 +
a slight participation by the ectoderm has not been satisfactorily excluded.
 +
Kastschenko's conclusions, however, as to the ectodermal origin of the
 +
thymus are imwarranted by his recorded observations. Prenant, after
 +
his careful work (on the sheep), was not sure that a small mass of ectoderm did not enter into the formation of the head. Practically all other
 +
investigators of this problem maintain that tlie ectoderm takes no part
 +
in the formation of the thymus. The epithelial l)ody (nodulus thymicus)
 +
developing in connection witli tlie head of the thymus from the third
 +
gill pouch does not form the carotid gland. Kastschenko's description of
 +
the origin of the carotid gland in mammals from the adventitia of the
 +
internal carotid is now accepted by the majority of anatomists, and it
 +
therefore has nothing to do with the thymus in oi'igin.
 +
 +
TiiK Hist<)L()(;y of tiik FrLLY-F()i;.Mi:i) Thymus.
 +
 +
Before taking u\) tlic histogenesis, I shall bi'iclly (.-(Uisider the histology
 +
of the gland as shown in a 24-cni. embryo. At this stage ihe gland nuiy
 +
be regarded as fully formed. As is well known, tlie thymic lobule consists of a cortical and a medidlary portion, — the medulla of all the
 +
lobules being united by the medullary cord. The cortex consists of a
 +
delicate reticulum with its spaces well filled by cells, usually lymphocytes.
 +
The reticulum may be regarded as composed of small branched anastom
 +
 +
 +
36 The Development of the Thymus
 +
 +
osing cells, though of eourse no cell houndaries are distinguishable. The
 +
nuclei are poor in chromatin, rounded, and usually 4.5 /a to 5.5 /x in
 +
diameter. The amount of cytoplasm around the nuclei and connecting
 +
them is usually very small. At some nodes there is a greater amount of
 +
cytoplasm giving the appearance of a large reticulum cell. In connection
 +
with the lilood-vessels, which are numerous in the cortex, are often found
 +
branched cells with pale nuclei and cytoplasm that stains more intensely
 +
than that of the rest of the reticulum. By a modification of Mallory's
 +
method used by Jackson (13, S. 39), I have been able to demonstrate
 +
numerous fibrillEe in the reticulum. In some cortical areas at this
 +
stage there are a great many erythroblasts. Masses of free erythrocytes
 +
are often found, usually near a comparatively large vessel, but such cells
 +
occur singly anywhere in the cortex.
 +
 +
In the medulla, the syncytial character of the stroma is much more
 +
pronounced. The cytoplasm is much more abundant than in the cortex,
 +
and the spaces are smaller and not so numerous. There is not so much
 +
room for lymphocytes as in the cortex, hence the lighter color of the
 +
medulla in stained preparations. The nuclei of the syncytium are either
 +
pale or dark, both types showing wide variations in size. By Jackson's
 +
method (13, S. 39), fibrilla3 may be readily demonstrated in the syncytium. In Plate I, Fig. 6, is shown the arrangement of these fibrillse
 +
(s f) in the medulla at 24 cm. They often may be traced into the
 +
areas which are forming the concentric corpuscles. In some parts of the
 +
medulla the fibrillae are very numerous ; in a few places, entirely absent.
 +
In both cortex and medulla eosinophile cells are often found. These
 +
occur in groups in the interlobular tissue around the blood-vessels, around
 +
some of the corpuscles of Hassall, and singly in the reticulum. These
 +
have been described by Schaffer (24). Free erythrocytes a.re rarely found
 +
in the medulla. In the medulla are also found the corpuscles of Hassall.
 +
Since the structure of these bodies depends largely upon their age, it may
 +
be better understood from the consideration of their development.
 +
 +
The Lymphoid Transformation.'
 +
 +
Kolliker, in 79, first advanced the idea that the leucocytes are formed
 +
directly from the epithelial cells of the thymic anlage. According to
 +
Minot (in human embryology, p. 878), "he records for the rabbit that
 +
between the twentieth and twenty-third days the cells of the thymus become smaller and their outlines disappear, so that the organ appears to
 +
 +
° This term will be used to include those changes that occur in the thymus
 +
during its passage from an epithelial to a characteristic lymphoid structure.
 +
 +
 +
 +
,E. T. Bell 37
 +
 +
bo ail atiuiiiulaticni of small round nuclei. At about the same period
 +
blood-vessels and eonneetive tissue grow into the epithelial anlage."
 +
 +
His {I'i 1)). 80, and Stieda (26), 81, claimed that the corpuscles of
 +
Hassall are the only remnants of the epithelial anlage, that the lymphocytes, reticulum, etc., are of mesenchymal origin.
 +
 +
Maurer (17 a), 86, described the leucocytes as arising directly from the
 +
cells of the epithelial anlage in the thymus of teleosts. In the amphibian
 +
thymus (17 b), 88, he thinks that the leucocytes are probably of mesenchymal origin. He was unwilling to conclude that they arose from the
 +
epithelium because he could not find transition forms. In lizards (17 c),
 +
99, he records that even before the separation of the epithelial anlage of
 +
the thymus from the pharynx, changes begin. The peripheral cells are
 +
closely crowded together and show many mitoses. There arises between
 +
the central cells, or is formed in vacuoles in their protoplasm, a fluid which
 +
accumulates until the nuclei surrounded by a thin zone of protoplasm are
 +
connected only by protoplasmic threads. A loose medulla is thus formed
 +
which looks like a cellular reticulum. The cortex is still solid. The
 +
lymphocytes are formed from the epithelial cells; none come from without. Later, blood-vessels and connective tissue grow in. He believes
 +
that the reticulum is of mesenchymal origin in all forms. Maurer
 +
(17 d), 02, still holds that in amphibians the lymphocytes are probably
 +
of mesenchymal origin.
 +
 +
Plermann et Tourneux (11), 87, find that in man and other mammals
 +
the epithelial anlage of the thymus is gradually converted into leucocytes
 +
and reticulum cells. Vacuoles appear during the transformation which
 +
seem to be formed by the absorption of large cells. In a sheep embryo
 +
of 130 mm., the clear epithelial cells have all disappeared, giving rise
 +
to small round cells and reticulum cells. Prolongations of connective
 +
tissue, each containing a blood-vessel, grow into the anlage during the
 +
transformation. They are not sure that all the thymic elements are
 +
epithelial in origin, being especially in doubt about the origin of some of
 +
the reticulum cells.
 +
 +
Gulland (8), 91, describes the development of the tonsil in the rabbit.
 +
Leucocytes first appear in the connective tissue around the thymus. Later
 +
they appear in the connective tissue around the tonsil. They infiltrate
 +
the tonsillar epithelium. Fo leucocytes are of epithelial origin. After
 +
studying the tonsil he examined the thymus in a few specimens and
 +
concluded that the same process of leucocyte infiltration occurred there.
 +
He does not give the details of their infiltration, and did not see any of
 +
the transition forms of nuclei in the thymus at that period.
 +
 +
Prenant (22), 94, made a careful study of the development of the
 +
 +
 +
 +
38 The Drvi'lopincnt ol' the 'riiynius
 +
 +
thynuis in sheep embrvos. His results are as follows. At 25 mm. the
 +
gland is composed of distinct polyhedral cells with nuclei of only one
 +
kind. A few mitoses and an occasional direct division are to be seen. At
 +
20 mm. mitoses are numerous (oi^e nucleus in fifty). jSTuclei are regularly rounded or elliptical and some small nuclei occur juxtaposed to
 +
large nuclei. At 28 mm. many mitoses are present and irregular spaces
 +
have appeared. These spaces are not blood-vessels nor parts of the
 +
thymic duet but vacuoles. Some nuclei, noticeably small and darkly
 +
colored, lie close to the large, clear nuclei and seem to be budded off from
 +
them. Some nuclei (rare) are broken into three or four chromatic
 +
bodies. Embryos of 40 mm. have undergone in great part the lymphoid
 +
transformation. All transitions are found between the large, pale elliptical nuclei of clear reticular structure and the small, deeply colored
 +
rounded nuclei whose sap is strongly stained. These last are certainly
 +
lymphocytes and constitute an immense majority of the cellular elements.
 +
Large, clear nuclei are found Joined to small dark ones — nuclear couples.
 +
 +
At 85 mm. the medulla appears; the cortex corresponds to the entire
 +
thymic mass of preceding stages. The cortex contains a great many
 +
lymphocytes separated by islands and rows of pale nuclei. There are
 +
about thirty lymphocytes to one pale nucleus. Mitoses are numerous in
 +
the cortex. In the medulla at this stage, the large clear, and small dark,
 +
nuclei are about equal in number, and mitoses are rarer than in the
 +
cortex. In later embryonic stages a clear peripheral zone is present
 +
where cell proliferation takes place. Mitoses are now more numerous in
 +
the medulla than in the cortex. It is probable that a certain number of
 +
the epithelial cells persist as reticulum cells in the fully-formed organ.
 +
 +
J. Beard (3 a), 94, (3 b), 99, thinks that the function of the thymus
 +
is to form the first leucocytes. He finds that in the skate the epithelial
 +
cells are converted early into lymphocytes which emigrate into the blood.
 +
There are many breaks in the gland where the lymphocytes escape in
 +
masses. The thymus is the only source of leucocytes until the other
 +
lymphoid organs are formed.
 +
 +
Ver Eecke (28), 99, finds that in the frog the epithelial thymus is
 +
invaded by lymphocytes and connective tissue. The epithelial cells are
 +
not destroyed but merely dispersed by the mesenchymal elements. He
 +
calls the resulting tissue lympho-epithelial. This idea of the commingling of the two tissues had already been advanced by Eettcrer.
 +
 +
Xusbaum and Prymak (20), 01, on teleosts, agree with j\Iaurer that
 +
the lymphocytes are of epithelial origin but disagree on the details of their
 +
formation. They find that the epithelial anlage is at first composed of
 +
cells with distinct lioundaries. It is not different from the epithelium
 +
 +
 +
 +
E. T. Bell 39
 +
 +
of the pharynx. Before any blood-vessels or connective tissue have invaded the organ, changes begin in. the central part. These changes consist in the breaking up of the cytoplasm so that the cells become
 +
branched and connected by delicate processes. These processes finally
 +
break apart leaving a nucleus surrounded by a thin layer of protoplasm —
 +
a lymphocyte. The peripheral epithelial layer multiplies rapidly, forming
 +
nuclei somewhat smaller and darker than their own. These nuclei become gradually changed into the nuclei of lymphocytes and break away
 +
from the other cells. All transitions are present between the large, clear
 +
epithelial nuclei and the lymphocytes. Blood-vessels and connective
 +
tissue grow in from the outside.
 +
 +
It appears from a survey of the literature that, of those who have
 +
studied the origin of lymphocytes in the mamalian thymus. His, Stieda,
 +
and Gulland have advocated the idea that they invade the gland from
 +
without, and that the original epithelial anlage persists only as remnants,
 +
the corpuscles of Hassall. They also consider the stroma of mesenchymal
 +
origin. On the other hand, Kolliker, Hermann and Tourneux, and Prenant, have described the lymphocytes as derived directly from the epithelial
 +
cells of the anlage. Hermann and Tourneux and Prenant ascribed a
 +
similar origin to part of the reticulum.
 +
 +
Xeither His, Stieda, nor Gulland made a detailed histological study
 +
of the changes that take place in the thymus during the transformation.
 +
They did not see the vacuolization of the cytoplasm, the changes in the
 +
epithelial nuclei, etc. — processes wdiich undoubtedly occur. Gulland
 +
made nearly all his observations on the tonsil and then from a superficial
 +
examination of the thymus concluded that the process is the same there.
 +
The conclusions of these men are therefore not to be compared on this
 +
point with those obtained by the thorough and careful work of Prenant.
 +
 +
On amphibians, Maurer hesitatingly agrees with His, and Yer Eecke
 +
accepts the mesenchymal origin of the leucocytes ; while on fishes Maurer,
 +
Beard, and Nusbaum and Prymak believe in the epithelial origin of
 +
lymphocytes. Maurer's work on reptiles is in agreement with his work
 +
on teleosts.
 +
 +
As to the origin of leucocytes in the lymphoid organs of tlie alimentary
 +
canal, opinion is divided. Eetterer, v. Davidoff, Rudinger, Klaatsch,
 +
and others have described the leucocytes as arising from epithelium and
 +
being invaded by mesenchymal elements forming adenoid tissue. Stohr,
 +
Gulland, Tomarkin, and others describe them as penetrating the epithelium from without.
 +
 +
I shall now discuss my own observations on the lymphoid transformation in the thymus of the pig. From a very early stage (11 mm.), the
 +
 +
 +
 +
40 The Development of the Thymus
 +
 +
t'pithelium of the third gill pouch is a syncytium. No cell boundaries
 +
exist. The nuclei, large and irregular in shape, are embedded in a common mass of cytoplasm. In the thymus at 20 mm. I find a syncytium
 +
of dense cytoplasm embedded in which are large nuclei of irregular shape
 +
and size. No distinct types of nuclei are present yet; all stain with
 +
medium intensity. A few mitoses are to be seen.
 +
 +
In a section of the mid-cervical segment at 3.7 cm. (Plate I, Fig. 1),
 +
I find evidence that the lymphoid transformation has begun. The syncytium is composed of coarsely reticulated cytoplasm much looser in
 +
texture than that of the preceding stage. It contains a few irregular
 +
spaces (s s) which are evidently of the nature of vacuoles. These may
 +
be formed, as Maurer suggests, by liquefaction of the cytoplasm. There
 +
is no reason to suppose that cells degenerate and form them as Hermann
 +
and Tourneux believed. Three types of nuclei may be distinguished;
 +
large pale nuclei (Ipn) large dark nuclei (I dn), and small dark nuclei
 +
(lymphoblasts) (Ih). Transition forms occur between these types.
 +
The large dark nuclei are intermediate forms between the pale nuclei
 +
and the lymphoblasts. A few mitoses (m) occur. No blood-vessels are
 +
present inside the anlage but they may be seen between the buds just
 +
outside. At this stage, the head and the thoracic segment have areas
 +
that are somewhat farther advanced than this. The intermediary and
 +
cervico-thoracic cords show no changes.
 +
 +
At a later stage than the above (Plate I, Fig. 2), in the thoracic
 +
segment of a 4.5-cm. pig, jthe spaces of the syncytium (s s) have increased greatly in number and size. The anlage is now a cellular reticulum. The large pale nuclei are somewhat less numerous than the
 +
dark nuclei and many have become angular, adapting themselves to the
 +
nodes of the syncytium. They contain less chromatin than in the
 +
preceding stage. Large dark nuclei and lymphoblasts are present; the
 +
lymphoblasts are much more numerous than in the preceding stage. A
 +
very few small dark nuclei are completely separated from the syncytium.
 +
These are lymphocytes. There are no lymphocytes in the connective
 +
tissue around the thymus or in the blood at this stage. I did not
 +
examine the tonsil or spleen at any stage. A few small blood-vessels are
 +
to be seen; their walls consist of endothelium only. There are more
 +
mitoses than at the preceding stage, but none happened to be present in
 +
the area shown in the figure. During mitosis, at all stages of development, except the early epithelial condition, the chromosomes are so
 +
closely packed that it is very difficult to distinguish them individually.
 +
 +
In a section through the mid-cervical segment of a 7-cm. pig
 +
(Plate I, Fig. 5), we see a stage somewhat later than the one shown in
 +
 +
 +
 +
E. T. Bell 41
 +
 +
Fig. 2. In various jiarts of the section lymphocytes (I) are completely
 +
formed. Tlie great majority of the small round nuclei are in the lymphoblast (l h) condition, i. e.,thcy are not yet completely separated from the
 +
syncytium. There are a few lymphocytes outside the thymus in the
 +
interlobular tissue in this region; around the head and the thoracic
 +
segment at 7 cm. they are numerous, these parts being in a later stage
 +
of transformation. I have never seen lymphocytes outside the thyfnus,
 +
where there were none inside it ; but they appear outside shortly after they
 +
are formed here. Those formed next the interlobular septa seem to pass
 +
out very early. Of course the lymphoblasts, which are distinguishable
 +
from the lymphocytes only by being imbedded in the syncytium, are to
 +
be seen in the thymus long before any appear outside.
 +
 +
At the stage shown in Fig. 5, a great many nuclei are in mitosis. I
 +
have not seen at any stage, the amitoses and nuclear couples described
 +
by Prenant for the sheep. In some parts of the section comparatively
 +
large solid epithelial areas occur. These are found as often in the
 +
central as in the peripheral part. Many of the pale nuclei are smaller
 +
than those shown in Fig. 2. The blood-vessels are somewhat larger and
 +
more numerous than those at 4.5 cm.
 +
 +
It is to be noted that the epithelial anlage does not at any stage become
 +
converted entirely into small round cells as many observers have stated.
 +
Distinctly pale angular reticular nuclei can always be seen.
 +
 +
In the mid-cervical segment at 8.5 cm. (Plate I, Fig. 4), a great many
 +
lymphocytes (/) are formed. These lie between the persisting epithelial
 +
cells which are now arranged in irregular cords and islands. In these
 +
epithelial masses, lymphoblasts may still be seen indicating that the
 +
formation of lymphocytes is still in progTCss. Many of the pale nuclei
 +
are now small. The heavy hsematoxylin stain in this case makes the
 +
nuclei darker than they would appear with an ordinary stain. A few
 +
nuclei are in mitosis.
 +
 +
This figure shows also the first appearance of the medulla {ind). The
 +
medulla is formed directly, as shown in the figure, from persisting parts
 +
of the epithelial syncytium. Certain centrally situated masses of this
 +
syncytium undergo changes of such a nature that they stain readily with
 +
cytoplasmic stains such as Congo red. In sections stained with hematoxylin and Congo red, the medulla is first recognized as a l)rightly
 +
colored area situated usually about the center of the lobule. Tlicse
 +
epithelial masses that give rise to the medulla seem to increase in size
 +
about the time of the change in staining capacity. The first differentiation of the medulla is chemical rather than morphological, for there are
 +
other persisting epithelial masses even larger than it in the same section
 +
 +
 +
 +
42 The Development of the Thymus
 +
 +
that do not react in the same way with the cytophismic stains. The
 +
meduna appears in the head and the thoracic segment at 7.5 cm. to 8 cm.
 +
All the gland except this small central area forms the cortex. Bloodvessels now reach 'all parts of the gland, but are still few in number. I
 +
cannot distinguish any wall except the endothelium on those actually
 +
inside the gland.
 +
 +
In a 9.5-cm. pig, the medulla is larger. It contains pale nuclei of
 +
various sizes, large dark nuclei, and lymphoblasts. Its spaces are smaller
 +
than those of the cortex. The early stages in the formation of the corpuscles of Hassall appear as soon as the medulla begins to form. The
 +
epithelial cords in the cortex have become less conspicuous, but are still
 +
forming lymphocytes. A few nuclei are in mitosis. Many blood capillaries may now be seen penetrating the gland from the periphery.
 +
These vessels run in the epithelial masses and have a wall of large
 +
endothelial cells which gives them the appearance of radiating cords.
 +
When these vessels first appear, as at this stage, they have only an
 +
endothelial wall. The blood-vessels grow in as small capillaries which,
 +
after their entrance, increase in size and branch ; they do not break in as
 +
large vessels surrounded by mesenchymal tissue. I am fairly sure that
 +
aside from the endothelial cells few or no mesenchymal cells come into
 +
the thymus. Around the greater part of the periphery of the gland is
 +
a solid zone of syncytium two or three nuclei deep which is in transformation like the epithelial cords inside. This zone, described by Prenant as a zone of proliferation, grows rapidly, as the frequent mitoses
 +
indicate. Its inner boundary is forming lymphocytes and reticulum cells.
 +
 +
In a 14-cm. pig, the lymphoid transformation is practically at an end
 +
except in the medulla. The peripheral zone of proliferation has disappeared. The cortex has about the same structure as at 24 cm., as
 +
previously described. In the medulla, lymphoblasts, large pale nuclei,
 +
and the large dark intermediate types are still present. There are a few
 +
mitoses here. It is very probable therefore that the formation of lymphocytes is still in progress in the medulla. The medulla at 2-i cm.
 +
shows a similar structure except that there are fewer lymphoblasts. These
 +
facts persuade me to regard the medulla as a center for lymphocyte
 +
formation at least as late as birth. Connective tissue fibrillae begin to
 +
appear in the gland along the large blood-vessels and the interlolmlar
 +
septa as early as 10.5 cm. They are only a little farther in at 16 cm. ;
 +
but near full term they are present in nearly all parts of the stroma.
 +
(See Plate I, Fig. 6.)
 +
 +
The above account may be summarized as follows: In the pig the
 +
epithelial syncytium of the thymic anlage becomes loosened up by the
 +
 +
 +
 +
E. T. Bell 43
 +
 +
formation of vacuoles in it. These vacuoles increase in number and
 +
size, converting the anlage into a cellular reticulum. While this vacuolization is in progress, the nuclei, which at first are of one kind with a
 +
medium amount of chromatin, ditferentiate into large clear, large dark,
 +
and small dark (lymphoblast) forms. The large dark nuclei probably
 +
divide by mitosis and form the lymphoblasts. The lymphoblasts gradually break loose from the syncytium, passing into its spaces and becoming
 +
lymphocytes. Shortly after lymphocytes begin to be formed, some of
 +
them pass out of the gland into the surrounding connective tissue. The
 +
lymphoid transformation begins in embryos of 2.5 cm. to 3 cm. and continues in the cortex until 13 cm. or 13 cm. In the medulla it is not complete at birth. Since the thymus increases greatly in size during this
 +
period the epithelial syncytium must grow rapidly. Lymphocytes are
 +
constantly being formed at the expense of the growing syncytium. A
 +
peripheral zone of proliferation is present from about 8 cm. to 13 cm.
 +
The medulla is formed as a chemical differentiation of certain centrally
 +
situated areas of the epithelial syncytium. The histological changes
 +
occur earlier in the head and thoracic segment than in the mid-cervical
 +
segment and very much earlier than in the cords. The reticulum of both
 +
cortex and medulla is practically all of e])ithelial origin. Some branched
 +
cells around the blood-vessels in the cortex may be of mesenchymal
 +
origin.
 +
 +
My reasons for regarding tlie lymphocytes as of epithelial origin are
 +
as follows :
 +
 +
A. The lympholilasts are true epithelial nuclei, because (1) there are
 +
numerous transition forms between them and the large dark nuclei which
 +
later cannot l)e regarded as invading lymphocytes; (3) they are closely
 +
embedded in the syncytium and show no evidence of having eaten their
 +
way through the protoplasm; (3) they are present from a very early
 +
stage and increase in number as development proceeds; (4) they are
 +
present before blood-vessels invade the gland and have no constant relation to blood-vessels or to the surface of the gland that indicates an invasion from either of these directions; (5) they are present before lymphocytes appear in the connective-tissue around the thymus.
 +
 +
B. Some observers admit tliat the small dark nuclei (lymphoblasts)
 +
are of epithelial origin but do not admit that tlicy form lym])hocytes.
 +
The considerations that lead me to bciicNc tliat the lymphoblasts do form
 +
the lymphocytes are: (1) the small dark nuclei (lymphoblasts) show
 +
every possible relation to the syncytium from being completely embedded
 +
in it to lying free in the syncytial s]")aces. A comparison with later
 +
stages shows tb.at tliis appearance is not due to poor fixation oi- to the
 +
 +
 +
 +
44 'V\\v ncvclopnioiit of tlio M'hymns
 +
 +
adherence of the nuclei to the reticuhini ; (?) the first free niiclci often
 +
appear in the center of the gland whoi tlicrc are no other free nuclei in
 +
the periphery at that level; (3) there is good evidence that lymphocytes
 +
emigrate from the thymns in large numbers. If we examine the thymus
 +
of a 7-cm. pig in serial sections we find that the lymphoid transformation
 +
is less advanced in the mid-cervical segment than in the head. In the
 +
mid-cervical segment there are a few lymphocytes in the interlobular
 +
tissue. In the lower end of the head where there are more lymphocytes
 +
inside the gland, lymphocytes pack the interlobular tissue and form a
 +
thin zone around the periphery of the gland. In the middle of the head
 +
where the transformation is far advanced, lymphocytes pack the interlobular tissue and form a thick zone around the entire gland. Indeed,
 +
in some sections, there are more l3T3iphocytes in the zone outside than
 +
are present inside the gland. If this zone of lymphocytes be passing into
 +
the gland, it is not easy to understand why it is formed from Avithin outwards, and why it is thickest where the greatest number of lymphoc3ies
 +
are already present inside. No satisfactory suggestion has yet been made
 +
as to why lymphocytes should thus suddenly pour into the thymus at a
 +
time when if present at all elsewhere they are rare. They do not come
 +
to break up the thymic epithelium, for that is already a reticulum before
 +
free cells are present (Fig. 3, Plate I). Where lymphocytes invade
 +
intestinal epithelium as in the tonsil they eat paths through it leaving
 +
spaces. The epithelial reticulum of the thymus is not formed in that
 +
way. On the other hand it is not difficult to believe that this zone of
 +
lymphocytes is formed l^y cells passing out the periphery of the thymus
 +
and that the gland thus contributes a great number of lymphocytes to the
 +
organism; (4) I have not been able to find lymphocytes in the connectivetissue around the th}Tuus before they are present inside. An invasion by
 +
way of the blood-vessels may be excluded, since the thick zone of lymphocytes formed around the gland shows that these cells either enter or leave
 +
it through the preiphery.
 +
 +
The Corpuscles of Hassall.
 +
 +
These bodies were first mentioned by Hassall ( 10 ) in 46, He speaks
 +
of them as being composed of mother cells which enclose the newlyformed daughter cells and nuclei. He thought the central mass was
 +
formed by the outer enclosing layers. He found bodies which lie regarded
 +
of the same nature in fibrous coagulations in the heart.
 +
 +
Virchow (29), 51, in a discussion of endogenous cell formation, compares Hassall's corpuscles to carcinoma pearls. He had about the same
 +
 +
 +
 +
E. T. Bell 45
 +
 +
conception of the nature of the corpuscles as Hassall. This oft-quoted
 +
comparison was therefore not based upon a deep insight into their nature.
 +
 +
Giinzburg (9), 57, did not advance beyond Hassall's conception that
 +
the central mass is formed by the peripheral layers.
 +
 +
Paulitzky (21), 63, described the center of the corpuscles as homogeneous or granular. They sometimes contain an elliptical nucleus, sometimes fat droplets. The larger ones have in the center several nuclei' or
 +
cell-like forms. The central part is formed from masses of epithelial
 +
cells. Connective tissue cells grow around them and are transformed into
 +
epithelial cells forming the peripheral part of the corpuscle.
 +
 +
The term "concentric corpuscles" was introduced by Ecker (6), who
 +
described them as arising directly from gland cells by fatty metamorphosis. He distinguished (1) simple corpuscles, round vesicles with
 +
thick concentric hulls, containing inside a fatty opalescent mass, and (2)
 +
compound corpuscles, which consist of several vesicles with a common
 +
hull. The peripheral layers of a corpuscle consist of flattened cells.
 +
 +
His (13 a, 12 b), 60, 80, described the corpuscles as consisting of an
 +
outer striated shell, probably composed of nucleated cells, and containing lymphocyte-like cells inside. He supposed them to be the original
 +
cells of the epithelial anlage which become entangled in the reticulum in
 +
some way. Their rapid growth in their narrow confines causes the concentric form.
 +
 +
Cornil et Eanvier (5), 69, considered the corpuscles as arising from
 +
the endothelium of blood-vessels and compared them to the spheres of their
 +
" Sarcome angiolithique."
 +
 +
This suggestion of a vascular origin, made by Cornil et Eanvier, was
 +
elaborated by Afanassiew (la), 77.
 +
 +
Afanassiew held that the corpuscles of Hassall arise from the endothelium of the smaller veins and capillaries. The endothelial cells increase in size, become cubical, and later fill the lumen of the vessel.
 +
During the proliferation of the endothelium, the blood-vessels break up
 +
into segments which are now nearly solid cords. These cords are at first
 +
connected to each other and to blood-vessels, but they soon break apart.
 +
The surest proof that the corpuscles are of vascular origin is that erythrocytes may be found inside them. Vascular injections, however, do
 +
not go into a corpuscle except in a very early stage, since the lumen is
 +
soon obliterated by the endothelial plugs. The corpuscles are formed
 +
entirely by the endothelial cells. Afanassiew worked on embryos of man,
 +
the rabbit, and the calf.
 +
 +
Stieda (26), 81, in sheep embryos, describes the epithelial mass of the
 +
 +
 +
 +
4G Tlie Dcv('lf)|)tn(Mit of the Tli\nms
 +
 +
thymic aulage as being broken up by ingrowing adenoid tissue. From
 +
50 mm. to 60 mm., there arc no hirge epithelial ceils; but later at 100 mm.
 +
ho iinds in the adenoid tissue large cells 9 /a to 15 /x in diameter, isolated
 +
or united in groups, Avhose protoplasm colors light-red with carmine.
 +
These large cells have a concentric structure. Some of them are enclosed
 +
bv large cells whose cytoplasm does not color with carmine, giving rise to
 +
a yellowish mass of irregular form and stratified appearance. In older
 +
embryos (250 mm.), the cellular masses are numerous but the large
 +
colored cells are rare. The yellowish masses are groups of the large cells
 +
which have undergone a transformation like that of the stratum corneum
 +
of the epidermis. Stieda considers the large colored cells which form
 +
the corpuscles as remnants of the epithelial anlage, although he admits
 +
that for a long period during development he found no trace of them.
 +
He explains the formation of the corpuscles in accordance with Cohnheim's hypothesis that most tumors arise from unused tissue remnants.
 +
 +
Ammann (2) 82, made most of his observations on human foetuses.
 +
He describes the corpuscles as arising from connective tissue. The corpuscles are cellular in structure and are formed of one, two, or three central cells around which a variable number of cells, increasing with age,
 +
are arranged like the coats of an onion. The corpuscles are formed from
 +
reticulum cells and leucocytes. Growth consists in the apposition of cells
 +
from without. The life of a corpuscle consists usually of four stages :
 +
(1) Stadium der Transparenz ; (3) Stadium der colloidcn Entartung;
 +
(3) Stadium der Verkalkung; (4) Stadium des Zerfalls. The nucleus
 +
of a reticulum cell or leucocyte increases in size at the expense of the
 +
cell body. Its increase in size establishes the concentric form. The
 +
corpuscle undergoes colloid and usually calcareous degeneration. Fat
 +
droplets, cholesterin crystals, and colloid granules are found together in
 +
the degenerating corpuscles. Breaking up in this way makes absorption
 +
possible. No epithelial remnants are to be observed. Xo erythrocytes
 +
are found in the corpuscles.
 +
 +
In four cases of atrophic thymus gland wliich yet contained lymphoid
 +
tissue Ammann found corpuscles in all stages of development. He also
 +
found that the corpuscles are formed most rapidly when the thymus is
 +
at the height of its development. From these facts he concluded that
 +
they are not connected with the involution of the thymus as Afanassiew
 +
thought. He thought that their formation is due to a physiological decrease in the intensity of growth of the medulla, due to the rapid growth
 +
of the cortex.
 +
 +
Watney (31), 83, agreed with Ammann that the corpuscles arise from
 +
connective tissue cells.
 +
 +
 +
 +
E. T. Bell 47
 +
 +
Monguidi (18), 85, distinguished true and false concentric corpuscles — •
 +
the latter being onl}- sections of blood-vessels.
 +
 +
Hermann et Tourneux (11), 87, gave a description of the structure
 +
and formation of the concentric corpuscles about like that given by
 +
Ammann except that they regard the reticulum cells from which the
 +
corpuscles develop as of epithelial origin.
 +
 +
Gulland (8), 91, regarded the corpuscles as epithelial remnants and
 +
compared them to the epithelial pearls of the tonsil.
 +
 +
Maurer (17 c), 99, described the corpuscles as epithelial in origin.
 +
His description of their formation is however different from that of His.
 +
All the cells of the epithelial anlage at first assume a lymphoid character.
 +
Later, some of these cells reassume their epithelial nature and then form
 +
the corpuscles. His conclusions for teleosts and amphibians are similar
 +
to the above results which he obtained from the lizard.
 +
 +
Yer Eecke (28), 99, for the frog, describes the leucocytes and connective tissue cells as invading the thymic anlage and separating the epithelial cells. The epithelial cells, separated by the mesenchymal elements,
 +
lie at first in groups or singly. They go through a cycle of two phases,
 +
a stage of growth, and a stage of involution. In the former stage, they
 +
increase to three or four times their original size and their cytoplasm
 +
differentiates into circular layers like the coats of an onion. The majority
 +
are monocellular. Some cells grow together making a more complex
 +
multicellular type. There are some intermediate forms, cells with a dense
 +
dark protoplasmic body, indistinct striations, and a nucleus partly or
 +
completely hidden in a precocious degeneration. In the stage of involution, which sets in early, the cytoplasm degenerates by the formation
 +
of vacuoles containing a hyaline liquid. The liquefaction may be in the
 +
form of a diffuse vacuolization, a large central vacuole, or a peripheral
 +
vacuole circular in section. The nucleus loses its affinity for stains, becomes deformed, breaks up, and finally disappears. The corpuscles are
 +
finally absorbed. They never contain erythrocytes. The cells do not degenerate to form a corpuscle. The liquefaction forms an internal secretion which is forced out by the muscle tissue in the reticulum.
 +
 +
Entirely different results on amphibians are reported by Nusbaum and
 +
Machowski (19), 02. These investigators revive the old idea of Afanassiew, accepting his results except that they think the adventitia as well
 +
as the endothelium of the blood-vessels takes part in the formation of the
 +
corpuscles. They find erythrocytes in the corpuscles. These erythrocytes
 +
either gradually shrivel and disappear, or they are absorbed by leucocytes
 +
or endothelial cells. The leucocytes after digesting the hemoglobin of the
 +
erythrocytes become eosinophile cells which are numerous in the thymus.
 +
 +
 +
 +
48 The Development of the Thymus
 +
 +
Wallisch (30), 03, measured the volume of the human thymus and of
 +
the corpuscles of Hassali at various stages. He finds that the total volume
 +
of the corpuscles of a 7-mo. embryo is 4.6 mm./ and of those of a 6-mo.
 +
child, 174.6 mm." The total volume of the thymus of a 78-mm. embryo,
 +
when it has already been partly transformed into adenoid tissue is only
 +
6.8 mm.^ Since there is no evidence that the cells of the corpuscles
 +
multiply, he concludes that they cannot be regarded merely as remnants
 +
of the original epithelial anlage.
 +
 +
Disregarding the crude observations of the earliest investigators, there
 +
remain three distinct theories of the formation of the corpuscles of
 +
Hassali.
 +
 +
1. The epithelial anlage of the thymus is broken up by the invading
 +
mesenchymal elements. The separated masses of epithelial cells undergo
 +
further changes mainly of a degenerative nature to form the corpuscles.
 +
This was the belief of His and KoUiker. According to this interpretation,
 +
the corpuscles are to be regarded as remnants that have nothing further to
 +
do with the gland. Stieda, Maurer, and Ver Eecke held this view in a
 +
modified form. Stieda regarded the cells forming the corpuscles as
 +
epithelial remnants but admitted that they go through a stage in which,
 +
for a time, they lose their epithelial character. This is substantially the
 +
same as ]\Iaurer's view. He thinks that the cells of the epithelial anlage
 +
all become lymphoid, and that some of them afterwards reassume their
 +
epithelial nature and form the corpuscles. Yer Eecke regards the corpuscles as epithelial remnants but thinks that they are glandular in nature,
 +
not mere useless remains.
 +
 +
2. The corpuscles are formed from the proliferating walls of bloodvessels. This idea was suggested by Cornil and Eanvier and elaborated by
 +
Afanassiew. Nusbaum and Machowski accept Afanassiew's view except
 +
that they believe the adventitia of the blood-vessels as well as their endothelium takes part in the formation of a corpuscle. These investigators
 +
thought that the formation of the corpuscles is connected with the involution of the thymus.
 +
 +
3. The corpuscles are formed from reticulum cells of the medulla
 +
and grow by apposition of the surrounding cells. This view was advanced
 +
by Ammann. Ammann thought that the reticulum is of connective tissue
 +
origin. He also believed that leucocytes formed the central part at least
 +
of some corpuscles. Hermann and Tourneux accepted Ammann's results,
 +
except that they ascribed an epithelial origin to the reticulum. (I do not
 +
know whether they accepted the origin from leucocytes described by
 +
Ammann.) Ammann thought that the corpuscles formed because of a
 +
physiological decrease in the rate of growth in the medulla.
 +
 +
 +
 +
E. T. Bell 49
 +
 +
l\Iy own observations on tlie development of tlie eorpuseles of Hassall
 +
in pi_o- embrvos. will now be considered. The medulla, as previouslv described, begins to form from the epithelial syncvtium usually near the
 +
center of the lobule. It is first recognized by its more marked reaction
 +
with cytoplasmic stains such as Congo red. Shortly after the medulla
 +
begins to form, the earliest stages of the corpuscles may be observed. - A
 +
few corpuscles have appeared at 9.5 cm. I did not find them earlier.
 +
They are all formed from the epithelial syncytium of the medulla.
 +
 +
Before beginning this discussion I will explain the use of ray terms.
 +
By a corpuscle of Hassall, I mean a modified area of the epithelial syncytium of the medulla, containing at some period of its development, one
 +
or more nuclei, and whose cytoplasm has been in part or entirely transformed into colloid. The term colloid is applied to various substances
 +
probably of widely different chemical nature, but is fairly adapted to our
 +
imperfect knowledge. I shall use the term here in the restricted sense
 +
employed by Ziegler," i. e., hyaline substances of epithelial origin, that do
 +
not give the reactions of mucin.
 +
 +
Colloid in the corpuscles of Hassall does not usually appear as solid
 +
masses in its early formation, but as fibers, granules, or sheets which are
 +
separated by more or less cytoplasm that is not yet changed. This stage
 +
I have called, "colloid in formation" (c f). It later assumes a more
 +
solid homogeneous appearance which I call solid colloid (c s). Often
 +
the solid colloid stains intensely with cyto])lasmic stains. I call this
 +
kind solid deeply-staining colloid (c .*? d). In later stages, the colloid
 +
often loses its affinity for cytoplasmic stains, staining a very pale color or
 +
not staining at all. I call this variety old colloid (o c).
 +
 +
According to their mode of development, the corpuscles of Hassall may
 +
be classified as follows :
 +
 +
A. Concentric Corpuscles.
 +
 +
a. Simple.
 +
 +
1. Ordinary type.
 +
 +
2. Epithelioid type.
 +
 +
3. Cystic ty.pe.
 +
 +
b. Compound.
 +
 +
B. Irregular Corpuscles.
 +
 +
a. Compact type.
 +
 +
b. Eeticular type.
 +
 +
*Gen. Pathology, 10th ed., Warthin's translation, p. 205.
 +
 +
 +
 +
50 Tlie Development of the Thymus
 +
 +
A. The concentric corpuscles inchule those that from their earliest
 +
appearance are concentric in structure. Adopting Ecker's classification,
 +
I distinguis^i simple concentric corpuscles and compound concentric
 +
corpuscles.
 +
 +
(a) Three types of simple concentric corpuscles are to be considered.
 +
(1) The ordinary type is far more numerous than any other. The earliest recognizable stage is shown in Plate II, Fig. 11. A nucleus (n)
 +
of the syncytium of the medulla has enlarged to perhaps twice its ordinary
 +
volume and has lost the ability to stain in the characteristic way with
 +
ha?matoxylin. Its sap is clear and a few reddish stained granules represent its chromatin. x\round it in the cytoplasm is an indistinct uneven
 +
layer of colloid (c /). The colloid is not yet solid and is being formed
 +
in concentric fibers or sheets. A slightly later stage is shown in Plate II,
 +
Fig. 14 and Fig. 15 (left side of figure). Some of the colloid (c s)
 +
next to the nucleus is now solid. The next stage is shown in Plate II,
 +
Fig. 15 (right side of figure). These corpuscles show a thick layer of
 +
colloid (c s d) that stains intensely with Congo red. Just outside the
 +
deeply staining colloid, colloid in formation may be seen. The nuclei
 +
are clear, and have become smaller and irregular in outline. The colloid
 +
seems to be pressing upon them and obliterating them. The colloid
 +
transformation gradually involves the adjacent c3^toplasm of the syncytium until other nuclei are involved. The corpuscle has now reached
 +
the condition shown in Plate II, Fig. 12. The central area (o c) is
 +
solid, the nucleus having disappeared entirely. Another (n') is nearly obliterated by the colloid. Part of the central area {o c) no longer stains
 +
intensely, and it is breaking loose by the formation of a concentric space.
 +
Several nuclei are surrounded by colloid in formation. Their long axes
 +
are nearly in a tangential direction. These nuclei are clear but only
 +
moderately swollen.
 +
 +
In the further development of the corpuscle (Plate III, Fig. 17 and
 +
Plate II, Fig. 7), the central area {c s d) increases in size. The nuclei
 +
involved in this area become obliterated probably by the pressure of the
 +
colloid and are no longer distinguishable. This central area usually splits
 +
off and may break up into many smaller masses. The peripheral part of
 +
the corpuscle increases by extension of the colloid formation into the
 +
adjacent part of the syncytium. This extension takes place in the early
 +
stages by direct progressive involvement of the immediately adjacent cytoplasm; in later stages (Fig. 7), by the formation of concentric lamellae
 +
which cut ofl' unchanged areas of cytoplasm. The lamelhp increase in
 +
size and number, the cytoplasm included between them is changed into
 +
colloid. They finally become closely packed, giving the characteristic and
 +
 +
 +
 +
E. T. Bell 51
 +
 +
well-kncnvn onion-like structure found in the fully-formed eorpusele.
 +
The nuclei that are enclosed between the lamells! gradually lose their
 +
chromatin and become flattened out. They do not swell and are not
 +
obliterated. It seems that swelling occurs only in nuclei that are surrounded by deeply staining colloid, and that this change is preparatory to
 +
their obliteration by or transformation into colloid. The amount of the
 +
corpuscle that breaks up to form the softer center is very variable. The
 +
size of the center usually seems to increase with the age of the corpuscle.
 +
 +
Plate III, Fig. 21, shows a variation from the ordinary concentric
 +
type. The central nucleus (n) stains reddish but is not enlarged. Most
 +
of the other nuclei are unchanged. All the colloid (c /") is in the early
 +
fibrous and granular stage.
 +
 +
From 20 cm. to full term nmny corpuscles show masses of calcareous
 +
material in or near the center. This material rarely appears in younger
 +
corpuscles (Plate III, Fig. 17, cl). It stains a violet blue with Delafield's ha?matoxylin.
 +
 +
The majority of the corpuscles of Hassall belong to the ordinary type
 +
of simple concentric cor])uscles described above. It is very clear that
 +
they have nothing to do with blood-vessels. They never contain erythrocytes nor anything resembling them. Earely a lymphoblast or leucocyte is found inside the corpuscle. These seem to be usually involved in
 +
the corpuscle like ordinary stronui nuclei during the formation of the
 +
lamellae. (Their occurrence in other types will be discussed later.) It
 +
is also clear that these corpuscles arise from the syncytium of the
 +
medulla. They are epithelial in origin, since the entire stroma of
 +
the gland is derived from epithelium, but they are certainly not remnants
 +
of the original epithelial anlage. Xeither are they forjned from lymphoidlike elements that reassume their epithelial nature as Maurer described
 +
for the lizard.
 +
 +
Some of Ammann's observations are in accord with my results. The
 +
swelling of the nucleus was noted by Ammann as tlu; first step toward the
 +
formation of the corpuscle. It should he noted, however, that rarely a
 +
corpuscle begins to form as a mass of colloid out in the cytoplasm and
 +
involves nuclei secondarily. I cannot distiiiguish his " Stadium der
 +
Transparenz " for I cannot be sure that a corpuscle is beginning to form
 +
until some colloid is present. The formation of the colloid is associated
 +
with the swelling of the nucleus. His other three stages, "Stadium der
 +
coUoiden Entartung," " Stadium der Yerkalkung," and " Stadium des
 +
Zerfalls " are easily seen. I have never seen corpuscles begin in leucocytes
 +
as x\mmann described. His statement thnt the corpuscle grows by apposition of reticulum cells is true in a modified sense. He thought that
 +
 +
 +
 +
52 Tln' I )L'V('lo[)iiu'iit of []]{' 'I'liymus
 +
 +
the outer ])art of a {•or])nscle is formed of reticuhim cells that have moved
 +
up and thittened themselves out around it. The description just given
 +
shows ihat tlie coi'puscles are iieNcr composed of distinct cells, and that
 +
the increase in size is due to an extension outward of tlie colloid foi'mation
 +
and not to a moving in of the adjacent tissue.
 +
 +
The concentric form of this type of corpuscle is due at first to its l)eing
 +
formed around a spherical or ellipsoidal nucleus. The swelling of this
 +
nucleus creates a centrifugal pressure in the adjacent cytoplasm. Before
 +
or during its transformation into colloid, the cytoplasm also increases in
 +
quantity\ That the cytoplasm increases in quantity is shown hy the fact
 +
that the nuclei are fewer in the corpuscle than in any adjacent area of
 +
the syncytium of equal size. This centrifugal pressure presses the newly
 +
formed colloid into concentric lamellae. It at first turns the long axes of
 +
the nuclei tangentially. and later flattens them and makes them concave
 +
toward the center.
 +
 +
3. The epithelioid type of corpuscle is characterized by large areas of
 +
cytoplasm so marked off by colloid lamella? as to give the appearance of a
 +
mass of large epithelial cells. They may contain only one nucleus embedded in a well-defined area of cytoplasm (Plate III, Figs. 18 and 20).
 +
These correspond to the monocellular corpuscles that have been described
 +
for lizards and amphibians. They are rare in the pig. I have not been
 +
able to trace these very far, as they soon become indistinguishable from
 +
other forms. The only difference I have noted is that the outer colloid
 +
lamellae begin to form early, causing the peculiar appearance of a large
 +
epithelial cell. Again the epithelioid type may present an appearance
 +
such as shown in Plate I, Fig. 3. These do not seem to be formed around
 +
any special nucleus. The outer colloid lamella? form before any center
 +
has been established, niarking off large cytoplasmic areas that may look
 +
like large cells. The centrifugal pressure of expansion caused by the
 +
great increase of cytoplasm in this area determines the concentric form
 +
in these corpuscles. Pure epithelioid corpuscles are very rare, but epithelioid areas in other corpuscles are not uncommon. The occurrence of
 +
epithelioid areas in corpuscles of the ordinary type shows that it is due
 +
to variations in a fundamentally similar process.
 +
 +
3. In the cystic type of corpuscle, the central part, instead of becoming
 +
transformed into colloid, undergoes early liquefaction, forming vacuoles.
 +
The central nucleus does not increase in size as in the ordinary type, but
 +
shrivels up and disappears. The corpuscle begins by the formation of
 +
outer colloid lamellae-^the central mass is not changed into colloid.
 +
In Plate II, Fig. 10, the central area (/; m) is undergoing a diffuse
 +
liquefaction. The nucleus (iv) is colorless and shrunken. In Plate TI.
 +
 +
 +
 +
E. T. Bell 53
 +
 +
Fig. 8 (right side of figure), the central area has fonned two lai-ge vacuoles (v). On the left side of the same figure, a concentric vacuole (v)
 +
has formed, separating off a central spherical nucleated mass of protoplasm. The nucleus of this mass of protoplasm is shrunken and the
 +
cytoplasm shows many small vacuoles. The corpuscle shown in Plate II,
 +
Fig. 9, is probably a later stage of the form just described. The central
 +
protoplasmic mass has become converted into an ellipsoidal pale bo'dy
 +
(pm). The small circular body in this shriveled mass is probably the
 +
nucleus. Some corpuscles like the one shown in Fig. 9 are found in
 +
wdiich the central mass has entirely disappeared. The further growth
 +
of corpuscles of this type seems to be by formation of colloid lamella? as
 +
in the ordinary type. They soon become indistinguishable from other
 +
forms.
 +
 +
The cystic type of corpuscle is rare in the pig. This evidently corresponds to the form in amphibia that misled Nusbaum and Machowski
 +
into reviving Afanassiew's theory. The central masses, in Figs. 8 and 9,
 +
might readily be mistaken for red corpuscles in animals in which these
 +
cells are nucleated. But the red cells of the blood of the pig are not
 +
nucleated at this stage. I have traced a number of these corpuscles (as
 +
well as those of other types) in serial sections and have never seen any
 +
indications of a connection to blood-vessels. Nusbaum and Machowski (19), (Fig. 1, c, S. 116) show a corpuscle which is similar to jny
 +
Fig. 9. It will 1)0 noted that the central space in neither of these figures
 +
is lined by endothelium. The early form of corpuscle shown by Nusbaum and Machowski (Fig. 1, d, S. 116) is very probably a normal bloodvessel with cubical endothelium. I have often found such vessels with
 +
cubical endothelium in the interlobular tissue of the pig's thymus at 10
 +
cm. to 12 cm. They probably may be found at other stages also. In the
 +
thymus of a kitten, injected l)y the intra-vitam Prussian blue method previously described, the majority of the corpuscles were found to be in
 +
early stages. The injection did not penetrate any corpuscle. I had a
 +
somewhat better opportunity to study the relations of the corpuscles to
 +
the blood-vessels in a 14 cm. human embryo. Here the vessels of the
 +
thymus were all very much distended with blood and the corpuscles were
 +
in early stages. No blood cells were found in the corpuscles.
 +
 +
(b) Compound concentric corpuscles are formed whenever two or more
 +
simple concentric corpuscles begin to form so close together that they
 +
come in contact during their later growth. An early stage of such a
 +
corpuscle is shown in Plate II, Fig. 15. The colloid lamellae are formed
 +
around each center until they come in contact; they are tlien formed
 +
around both centers. In Plate III, Fig. 22, a compound concentric cor
 +
 +
 +
5-t Tlio D(>\('l()|)in('iit of the Tliyiinis
 +
 +
piisclc is shown. 'I'lici'c aiT three siiiiph' coiu'cntric corpuscles in it —
 +
one of them (the h>\vcst in the liyiii-c) in a very early stage. Several
 +
lamella' ai'c common lo the older cor|)uscles, and one is common to all
 +
three. This ari'an<i'ement of the lamella' is a mechanical efTect of the
 +
tension in the cytoplasm, due to the ccntrifug^al pressure from the two
 +
centers. The size of the separate centers in a compound corpuscle depends upon the stage they have reached when they come in contact. If
 +
a compound coi'puscle he formed hy the union of two simple corpuscles
 +
in an early stage, as in Plate 111, Fig. 19, all indications of its compound nature are soon lost. A corpuscle originally compound may, then,
 +
in later stages, hecome indistinguishal)Ie fi'om simple corjniscles. The
 +
simple corpuscles uniting to form a compound concentric cor]niscle may
 +
be of any of the types previously described.
 +
 +
B. Irregular Corpuscles.
 +
 +
This grou]) includes those corpuscles which are not at first concentric.
 +
Concentric areas may appear later. According to the classification previously given, I distinguish a compact type and a reticular type.
 +
 +
(a) The compact type (Plate III, Fig. 16) first appears as a compact
 +
area of syncytium of irregular shape. It is recognizable l)y the colloid
 +
it contains. The nuclei are not noticeably increased in size and have no
 +
regular arrangement. Their chromatin still stains dark with nuclear
 +
stains. The colloid (c f) is not yet solid. The corpuscle has no distinct
 +
center. These corpuscles grow by direct colloid transformation of the
 +
adjacent syncytium. No distinct lamella^ are formed. The colloid may
 +
remain in the fibrous condition shown in the figure (c f) or it may
 +
become solid, but it never reaches the deeply staining condition unless a
 +
concentric area be established.
 +
 +
A later stage of this tyi)e is shown in Plate III, Fig. 23. The corpuscle is sharply marked off from the syncytium. Some of its colloid is
 +
solid. A concentric area (cs) is beginning to form. The nuclei are
 +
not markedly different from those of the adjacent syncytium. These
 +
corpuscles may become large and branched. Often one or more concentric areas are developed after the corpuscle has attained considerable
 +
size. By the growth of these concentric areas, irregidar corpuscles may
 +
become converted into concentric corpuscles.
 +
 +
(b) The reticular type is produced by colloid formation in the ordinary reticulum of the medulla. In the types previously described, the
 +
spaces of the reticulum are \isually obliterated as the colloid formation
 +
advances; but in this form the spaces persist as a part of the corpuscle.
 +
Pure reticular corpuscles vary greatly in size, sometimes involving only
 +
 +
 +
 +
E. 'W Bell ■ 55
 +
 +
one node of the syncytium. Thev are never concentric, and never form
 +
lamellae. Eeticnlar areas often occur in other forms of corpuscles. In
 +
tiiis way leucocytes are often involved in tlic corpuscle, since they lie in
 +
the spaces of the reticulum. Lymphocytes often get into a corpuscle in
 +
the lymphohlast condition, heing cut off hy tlio formation of lamellae
 +
outside tliem (Plate III, Fig. 22). The Icui'Dcytes shut in the cor])uscle in this way during development uuiy not degenerate. They probably persist and help to remove the corpuscle in its final stages of degeneration.
 +
 +
The amount of expansion of the cyt()i)lasm before or during the colloid
 +
transformation is probably small in the irregular reticular corpuscles,
 +
since it does not obliterate the spaces of the syncytium. In the compact
 +
type, the spaces of the syncytium are obliterated and there is evidence
 +
of some expansive force (note the arrangenunit of the nuclei in the
 +
upper part of Fig. 23, Plate III). In the figure referred to, the number
 +
of nuclei in any part of the corpuscle is less than in an equal area of the
 +
adjacent reticulum. These facts indicate that there is an expansion of
 +
the cytoplasm. That this expansive force does not produce a concentric
 +
form is due primarily to the fact that there is no expansion of a nucleus
 +
and distinct center of formation as is present in concentric corpuscles of
 +
the ordinary type. The absence of the onion-like structure in irregular
 +
corpuscles is due to the fact that the colloid is not laid down in lanielUw
 +
 +
Significance of the corpuscles of Hassall. It has been shown in the
 +
preceding pages that the corpuscles of Hassall in the pig are not epithelial remnants, and also that they are not formed from blood-vessels.
 +
There is no evidence connecting their development with the involution
 +
of the thymus, for they begin to form before the lymphoid transformation is complete and are most numerous when the thymus is at the height
 +
of its development. I have not been al)le to see the decrease in the rate
 +
of growth of the medulla described by Amnumn, and even if such did
 +
occur it is difficult to understand how it could cause the formation of a
 +
corpuscle.
 +
 +
The above theories are, therefore, inconsistent with the facts of development in the pig. It seems to me that the formation of a corpuscle is
 +
not to be regarded as a ]u-ocess of degeneration. The fact that the formation of colloid is an essential feature in the development of every
 +
corpuscle is a strong argument that it is a form of secretion such as
 +
occurs in its neighl)oring branchial derivative, the thyroid. The fact
 +
that the corpuscles differentiate in an aj^parently uniform syncytium is
 +
further evidence airainst a theory of degeneration. Since the lymphocyte-forming fuiu-tion of tbc tliymus is probaI)ly secondary, it is not
 +
 +
 +
 +
56 The Dov(^lopin(<Tit of tlio Thymus
 +
 +
unreasonable to suppose that its primitive fimetion was the formation
 +
of a colloid secretion such as occurs in the thyroid, and that the corpuscles
 +
are abortive expressions of this primitive function/
 +
 +
Giant Cells.
 +
 +
Polykaryocytes may often 1)0 seen in the medulla. Tliese bodies develop froui the syncylium of the uieilulla. They are first noticeable as
 +
groups of small s])herieal nuclei in a solid area of the syncytium. Tliese
 +
nuclei stain with merlium intensity and are all very similar in size and
 +
color. The area containing this group of nuclei becomes a well-defined
 +
node of the reticulum and persists as such. A polykaryocyte is, therefore, a large node of the reticulum containing a number of small nuclei
 +
very similar in appearance. These cells often occur in groups. They
 +
are entirely distinct from the corpuscles. They are evidently similar
 +
to the polykaryocytes found in bone marrow and other lymphoid tissues.
 +
 +
Summary.
 +
 +
The following is a resume of the development of the thymus in the
 +
pig :
 +
 +
The thymus of the pig is probably developed entirely from the endoderm of the third gill pouch.
 +
 +
By a gradual process of vacuolization and liquefaction of the cytoplasm, the epithelial syncytium of the thymic anlage is converted into a
 +
cellular reticulum.
 +
 +
From the first appearance of vacuolization, three types of nuclei are
 +
present: large pale nuclei; small dark nuclei (lymphoblasts), and large
 +
dark intermediate forms.
 +
 +
The lymphoblasts gradually break loose from the cellular reticulum,
 +
moving into its spaces and forming lymphocytes. Mitoses are most numerous at the period of the most rapid formation of lymphocytes. The
 +
medulla continues to form lymphocytes at least as late as birth.
 +
 +
Lymphocytes appear in the connective tissue aromul tlu> th.ynuis
 +
shortly after they are formed ; and lymphoblasts, wdiich are distinguishable from lymphocytes only by being embedded in the syncytium, arc
 +
present in the thymus a long period before lymphocytes are found anywhere in the neighborhood of the thymus.
 +
 +
The celhdar reticulum of the earlier stages persists in a modified form
 +
as the reticulum of both cortex and medulla. It retains more cytoplasm
 +
 +
' Ver Eecke (28) believes that the corpuscles in amphibians are of a
 +
glandular nature.
 +
 +
 +
 +
E. T. Bell 57
 +
 +
in tlio inedulla. Practically all the reticnhira of both cortex and nie(liilla. as well as the lymphocytes, are, therefore, of epithelial origin.
 +
 +
The {■oi-])iisclos of Hassall develop from the syncytium and arc, therefore, epithelial in origin. They are, however, not to be considered as
 +
remnants of the original epithelial anlage.
 +
 +
In development various types of corpuscles are distinguished. Tlie
 +
ordinary typo of concentric corpuscles first appears as an enlarged clear
 +
nucleus around which colloid is being formed. Before or during the
 +
formation of colloid, the cytoplasm increases in quantity, filling the
 +
spaces of the reticulum and producing a centrifugal pressure which
 +
shapes the newly-formed colloid into concentric lamellse and flattens the
 +
neighboring nuclei, making them concave toward the center. The central nuclei usually become obliterated.
 +
 +
The epithelioid type is distinguished by its resemblance to large epithelial cells, this appearance being due to the formation of colloid
 +
lamella" around largo areas of clear cytoplasm. The central part of the
 +
corpuscle usually remains unchanged until after some of the colloid
 +
lamellfE are formed.
 +
 +
The cystic type differs from the ordinary type only in that the central
 +
part undergoes vacuolization instead of colloid transformation. Those
 +
with concentric vacuoles may simulate blood-vessels containing nucleated
 +
red cells. Corpuscles never contain erythrocytes; neither can they be
 +
injected at any stage of development. Serial sections also show tliat
 +
there is no connection to blood-vessels at any stage.
 +
 +
Compound concentric corpuscles are formed by the union of two or
 +
more simple concentric corpuscles during development.
 +
 +
Irregular corpuscles are not concentric ' in arrangement, and are
 +
formed in the syncytium in an irregular manner. In the compact type
 +
of irregular corpuscles, concentric areas may form.
 +
 +
The formation of colloid is an essential feature in the development of
 +
every corpuscle, and is not to be considered as a process of degeneration.
 +
 +
Since the conclusion of my work and after my manuscript was given to the
 +
publishers, two articles dealing with the thymus have appeared.
 +
 +
Ph. Stohr (Ueber die Thymus, Sitzungsberichte der phys.-med. Gesellschaft
 +
zu Wiirzburg, June 8, 1905) believes that the thymus first epithelial in nature
 +
becomes converted entirely into small cells of lymphoid appearance. Later
 +
the large reticulum cells are formed from these by enlargement. The corpuscles of Hassall are formed by the massing together and enlargement of
 +
these lymphoid-like cells. The small round cells of the gland are epithelial
 +
in origin but are to be regarded not as lymphocytes but as epithelial cells.
 +
The thymus is not a source of lymphocytes.
 +
 +
 +
 +
58 ''J'lu^ Devolopmont nf the Tliymiis
 +
 +
The author apparently believes that none of the small rounrt cells leave
 +
the gland though he admits that lymphocytes enter. But as mentioned above
 +
the zone of connective tissue immediately around the head at 7 cm. may
 +
contain even more lymphocytes than are present inside the gland at that time
 +
If these are all entering the gland then it is probable that most of the small
 +
round cells are really lymphocytes. This conception then does not simplify
 +
the problem but is only a theoretical compromise between the two views a'i
 +
to the origin of the lymphocytes.
 +
 +
J. Aug. Hammar (Zur Histogenese und Involution der Thymusdriise, Anat.
 +
Anz. Bd. XXVII, June 17, 1905) regards the reticulum as formed from the
 +
epithelial anlage but thinks the evidence at hand insufficient to decide the
 +
question as to the origin of the lymphocytes. He finds lymphocytes outside
 +
the thymus in many animals (man, cat, chick, frog) before any are present
 +
inside the gland. The corpuscles of Hassall develop from the epithelial
 +
reticulum and undergo hyaline (colloid?) degeneration.
 +
 +
My description of the formation of the corpuscles of Hassall differs essentially from Hammar's, in that I believe the formation of the corpuscle consists
 +
in the expansion of the cytoplasm of the syncytium and its conversion into
 +
colloid. Hammar did not recognize " colloid in formation," though he speaks
 +
of the coarse fibrillar structure of the protoplasm. He -did not describe such
 +
corpuscles as are shown in Fig. 7, Plate II.
 +
 +
The considerations presented above in favor of the epithelial origin of the
 +
lymphocytes seem to me much stronger than those given by Hammar. His
 +
statements as to the presence of lymphocytes around the thymus before they
 +
are present inside are to be taken with some reservation inasmuch as he
 +
mentions small round cells separate from the syncytium earlier, but regards
 +
ihem as degenerating epithelial cells (S. 65). His figure from the human
 +
foetus (Fig. 18, S. 66) does not seem to be strong support for his statement.
 +
Certainly many lymphocytes are present in the pig thymus when the reticulum is broken up as much as shown in the figure referred to. It is also to
 +
be borne in mind that the different parts of the thymus undergo the lymphoid
 +
transformation at different times and that a single section may therefore be
 +
misleading.
 +
 +
LITERATURE.
 +
 +
la. Afanassiew, B. — Ueber die concentrischen Korper der Thymus. Archiv
 +
 +
f. mikr. Anat., Bd. XIV, 1877.
 +
lb. Weitere Untersuchungen iiber den Bau und die Entwickelung
 +
 +
der Thymus und der Wintcrschlafdriise der Saugethiere. Archiv f.
 +
 +
mikr. Anat., Bd. XIV, 1877.
 +
2. Ammann, a. — Beitrage zur Anatomic der Thymusdriise. Basel, 1882.
 +
3a. Beard. — The development and probable function of the thymus. Anat.
 +
 +
Anz., Bd. IX, 1894.
 +
3b. The true function of the thymus. Lancet, 1899.
 +
 +
4. Born, G.— Ueber die Derivate der embryonalen Schlundbogen und
 +
 +
Schlundspalten bei Saugethieren. Archiv f. mikr. Anat., Bd. XXII,
 +
1883.
 +
 +
5. CoRNiL et Ranvier. — Manuel d'histologie pathologique. Paris, 1869, p.
 +
 +
135 (cited from Ammann).
 +
 +
 +
 +
E. T. Bell 59
 +
 +
6. EcKER. — Art. " Blutgefassdrusen," Wagner's Handw. der Phys., Ill (cited
 +
 +
from Ammann).
 +
 +
7. Priedleben, a. — Die Physiol, der Thymusdriise. Frankfurt, 1858.
 +
 +
8. GULLAND. — The Development of adenoid tissue with special reference
 +
 +
to the tonsil and thymus. Laboratory Reports issued by the Royal
 +
College Phys., Edinburgh, Vol. Ill, 1891.
 +
 +
9. GiJNZBURG. — Ueber die geschichteten Korper der Thymus. Zeitschr. f.
 +
 +
klin. Med., Bd. VI, 1857, S. 456 (cited from Henle und Meissner.
 +
Bericht iiber die Fortschritte der Anat. u. Physiol.).
 +
 +
10. Hassall. — The microscropical anatomy of the human body in health and
 +
 +
disease. London, 1846 (cited from Ammann).
 +
 +
11. Hermann et Tourneux. — Article thymus, Diet, encycl. des Sciences Medi
 +
cales. Troisieme Serie, 17, 1887.
 +
12a. His, W.— Zeitschrift f. wiss. Zoologie, Bd. X, S. 348. Leipzig, 1860.
 +
12b. Anatomic menschlicher Embryonen. Leipzig, 1880, S. 56.
 +
 +
13. Jackson, C. M. — Zur Histologie und Histogenese des Knochenmarkes.
 +
 +
Archiv f. Anat. und Physiol., Anat. Abth., 1904.
 +
 +
14. Kastschenko. — Das Schicksal der embryonalen Schlundspalten bei
 +
 +
Saugethieren. Archiv f. mikr. Anat., Bd. XXX, 1887.
 +
 +
15. Klein. — Neuere Arbeiten iiber die Glandula Thymus. Centralbl. f. allg.
 +
 +
Pathol, u. pathol. Anat., 1898.
 +
 +
16. Langerhans und Savei.iew. — Beitrage zur Physiologic der Brustdriise.
 +
 +
Virchow's Archiv, Bd. 134, 1S93.
 +
17a. Maurer. — Schilddriise und Thymus der Teleostier. Morph. Jahrb.. Bd.
 +
 +
XI, 1886.
 +
17b. Schilddriise, Thymus, und Kiemenreste bei Amphibien. Morph.
 +
 +
Jahrb., Bd. XIII, 1888.
 +
17c. Schilddriise, Thymus, und andere Schlundspaltenderivate bei der
 +
 +
Eidechse. Morph. Jahrb., Bd. XXVII, 1899.
 +
17d. In Hertwig's Handbuch der Entwickelungslehre der Wirbelthiere,
 +
 +
Lief. 6-8, S. 131 ff., 1902.
 +
 +
18. MoNGUiui. — Sulla glandula timo. Parma, 1885 (cited from Prenant).
 +
 +
19. NusBAUM, J., und Maciiowski. — Die Bildung der concentrischen Korper
 +
chen und die phagocytotischen Vorgange bei der Involution der
 +
Amphibienthymus, etc. Anat. Anz., Bd. XXI, 1902.
 +
 +
20. NusBAUM, J., und Prymak, T.— Zur Entwickelungsgeschichte der lym
 +
phoiden Elemente der Thymus bei den Knochenfischen. Anat. Anz.,
 +
Bd. XIX, 1901.
 +
 +
21. Paulitzky. — Disquis. de stratis glandulse thymi corpusculis. Habilita
 +
tionsschr., Halis, 1S63 (cited from Henle und Meissner's Bericht iiber
 +
die Fortschritte der Anat. und Physiol.).
 +
 +
22. Prenant. — Developpement organique et histologique du thymus, de la
 +
 +
glande thyroide, et de la glande carotidienne. La Cellule, Tome X,
 +
1894.
 +
 +
23. Prymak. T. — Beitrage zur Kenntnis des feineren Baues und der Involu
 +
tion der Thymusdriise bei den Teleostieren. Anat. Anz., Bd. XXI,
 +
1902.
 +
 +
 +
 +
60 'I'lio Dovclnpmont of the T'hynms
 +
 +
24. SciiAKFER, J. — Ueber den feirieren Bau der Thymus und deren Beziehung^
 +
 +
zur Blutbildung. Sitzungsber. d. K. Acad. d. Wissensch. Math.naturw. Kl. Wien., Bd. CII, Abt. Ill, 1893.
 +
 +
25. SciiEnEL, J. — Zellvermehrung in der Thymusdriise. Archiv f. mikr.
 +
 +
Anat., Bd. XXIV.
 +
 +
26. Stieda, L. — Untersuchungen uber die Entwickelung der Glandula Thy
 +
mus, Glandula Thyroidea, und Glandula Carotica. Leipzig, 1881
 +
(cited from Hermann et Tourneux).
 +
 +
27. Sx'LTAN.^Beitrag zur Involution der Thymusdriise. Virchow's Archiv,
 +
 +
Bd. 144, 189G.
 +
 +
28. Vek Eecke. — Structure et modifications fonctionelles du thymus de la
 +
 +
grenouille. Bulletin de I'Acadc'mie royale de MC'dicine de Belgique,
 +
1899.
 +
 +
29. ViKciiow, R. — Kritisches iiber den oberschlesischen Typhus. Archiv, Bd.
 +
 +
3, 1851, S. 222.
 +
 +
30. Wallisch. — Zur Bedeutung der Hassall'schen Korperchen. Archiv f.
 +
 +
mikr. Anat., 1903.
 +
 +
31. Watxey. — The minute anatomy of the thymus. Philos. Transact, of the
 +
 +
Royal Society of London, Vol. 173, Part III, 1883 (cited from Prenant).
 +
 +
EXPLANATION OF PLATES.
 +
 +
All the figures were drawn with Leitz obj. 1/12, oc. 4, and camera lucida.
 +
The magnification after the reduction of the plates is about 1060 diameters.
 +
All drawings were made from transverse sections of the mid-cervical segment of the thymus unless they are otherwise indicated.
 +
 +
The following abbreviations designate the structures indicated in all the
 +
figures :
 +
 +
c f — colloid in formation. I p n — -large pale nucleus.
 +
 +
cl — -calcareous deposit. m — nucleus in mitosis,
 +
 +
c s — solid colloid. vid — beginning of medulla,
 +
 +
c s d — solid colloid that stains n — nucleus.
 +
 +
deeply. o c — old colloid.
 +
 +
e — erythrocyte. p m — protoplasmic mass.
 +
 +
end — -endothelial nucleus. sf — fibril in syncytium.
 +
 +
I — lymphocyte. ss — space in syncytium.
 +
 +
Z& — lymphoblast. v — vacuole.
 +
I d n — large dark nucleus.
 +
 +
Plate I.
 +
 +
Fig. 1. From a 3.7-cm. pig embryo. Stained with iron-lijematoxylin and
 +
Congo red. Vacuolization of the cytoplasm and differentiation of the nuclei
 +
have begun.
 +
 +
Fig. 2. From the thoracic segment of a 4.5-cm. pig embryo. Stained with
 +
iron-haematoxylin and Congo red. A cellular reticulum is now formed. Large
 +
pale nuclei, lymphoblasts, and the large dark intermediate forms are present.
 +
 +
Fig. 3. Epithelioid type of concentric corpuscle. From a 16-cm. pig embryo. Stained with hajmatoxylin and Congo red. Colloid lamellae (c s d)
 +
 +
 +
 +
E. T. Bell 61
 +
 +
separate large areas of cleai- cytoplasm, causing the appearance of large
 +
epithelial cells. Colloid is being formed between the lamellae and around
 +
several nuclei.
 +
 +
Fit;. 4. From a 8.5-cm. pig embryo. Stained with iron-hsematoxylin (not
 +
decolorized). The medulla has appeared. Lymphocytes are present between
 +
the epithelial cords.
 +
 +
Fig. 5. From a 7-cm. pig embryo. Stained with iron-htematoxylin and
 +
Congo red. A few lymphocytes have been formed. In the cellular reticulum
 +
are large pale nuclei, lymphoblasts, and large dark intermediate nuclei. The
 +
nuclei in mitosis are very compact.
 +
 +
Fig. 6. From the medulla of a 24-cm. pig embryo. Stained with Jackson's
 +
modification of Mallory's method (ref. in text). Many fibrilte are seen in
 +
the syncytium.
 +
 +
Plate II.
 +
 +
Fig. 7. Ordinary type of simple concentric corpuscle. From a 16.5-cm.
 +
pig embryo. Stained with hsematoxylin and Congo red. The corpuscle is
 +
well advanced in development. Concentric lamellag of colloid have been
 +
formed. The cytoplasm between the lamellse is in an early stage of colloid
 +
transformation. Colloid fibers cut transversely appear as dots. The nuclei
 +
are becoming flattened by the pressure of expansion. The central mass stains
 +
irregularly and all traces of the nuclei in that region are gone.
 +
 +
Fig. 8. Two cystic concentric corpuscles. From a 16-cm. pig embryo.
 +
Stained with iron-hgematoxylin and Congo red. On the left, a nucleated
 +
mass of protoplasm has been separated off by the formation of a vacuole
 +
annular in section. This might be mistaken for a blood-vessel containing a
 +
nucleated red cell. In this central protoplasmic mass the nucleus is shrunken
 +
and the cytoplasm vacuolated. In the small corpuscle on the right, two large
 +
vacuoles have formed.
 +
 +
Fig. 9. Cystic concentric corpuscle. From a 14-cm. pig embryo. Stained
 +
with hsematoxylin and Congo red. The central protoplasmic mass is pale
 +
and shrunken. The small circular body in it probably is the remains of
 +
the nucleus. Colloid lamellae are forming. Colloid fibers cut transversely
 +
appear as dots.
 +
 +
Fig. 10. Cystic concentric corpuscle. From a 10.5-cm. pig embryo.
 +
Stained with hsematoxylin and Congo red. The center contains no colloid
 +
and seems to be softening. The nucleus is shrunken.
 +
 +
Fig. 11. Ordinary concentric corpuscle in a very early stage. From a
 +
l{j.5-cm. pig embryo. Stained with iron-ha'matoxylin and Congo red. The
 +
nucleus is enlarged and colloid is forming around it. A few colloid fibers
 +
may be seen in the cytoplasm for some distance from the central nucleus.
 +
 +
Fig. 12. Ordinary concentric corpuscle. Several nuclei are involved.
 +
From a 10.5-cm. pig embryo. Stained with hsematoxylin and Congo red. The
 +
deeply-staining colloid has completely obliterated the central nucleus (in the
 +
region o c), and nearly obliterated another («'). Some of the colloid now
 +
stains pale (o c).
 +
 +
Fig. 13. Ordinary concentric corpuscle. . From a 10.5-cm. pig. Stained
 +
with hiematoxylin and Congo red. The central nucleus is being obliterated
 +
 +
 +
 +
(52 The I)i'V('l()})iiHiit nf the Tlivnius
 +
 +
by the deeply-staiuing colloid. The neighboring nuclei are beginning to
 +
show the effect of the centrifugal pressure.
 +
 +
Fig. 14. Ordinary concentric corpuscle in an early stage. From a 10.5-cm.
 +
pig. Stained with haematoxylin and Congo red. A band of deeply-staining
 +
colloid has been formed. Just outside this is colloid in formation.
 +
 +
Fig. 15. Two simple concentric corpuscles which would have formed a
 +
compound concentric corpuscle. From a 10.5-cm. pig. Stained with haematoxylin and Congo red. The left corpuscle is a little more advanced than
 +
Fig. 11. The right corpuscle shows a large area of deeply-staining colloid
 +
which has pressed the nucleus into a small Irregular shape.
 +
 +
Plate III.
 +
 +
Fig. 16. Compact irregular corpuscle In an early stage. From a 14-cm.
 +
pig embryo. Stained with haematoxylin and Congo red. The colloid is not
 +
yet solid. The nuclei are not essentially different from those of the adjacent
 +
syncytium.
 +
 +
Fig. 17. Ordinary concentric corpuscle. From a 12-cm. pig embryo.
 +
Stained with haematoxylin and Congo red. There is a large, central, deeplystaining colloid mass in which calcareous deposits (c?) have been made.
 +
The neighboring nuclei show the effects of the centrifugal pressui-e.
 +
 +
Fig. is. Epithelioid concentric corpuscle in an early stage. From a 10.5cm. pig embryo. Stained with haematoxylin and Congo red. The outer colloid lamella marks off a nucleated mass of cytoplasm resembling a large cell.
 +
The nucleus is undergoing the same changes as occur in the central nucleus
 +
of an ordinary concentric corpuscle.
 +
 +
Fig. 19. Compound concentric corpuscle. From a 10.5-cm. pig embryo.
 +
Stained with haematoxylin and Congo red. This would have soon lost all
 +
evidence of its compound nature.
 +
 +
Fig. 20. Epithelioid concentric corpuscle. From a 10.5-cm. pig embryo.
 +
Stained with hematoxylin and Congo red. Some colloid is forming outside
 +
the circular area. Solid deeply-staining colloid is forming.
 +
 +
Fig. 21. Ordinary concentric corpuscle, showing a variation from the
 +
usual type. From a 16.5-cm. pig embryo. Stained with iron-haematoxylin
 +
and Congo red. The central nucleus is reddish but not enlarged. No solid
 +
colloid has been formed.
 +
 +
Fig. 22. Compound concentric corpuscle. From a lG.5-cm. pig embryo.
 +
Three centers are present. The pale colloid in the upper part is probably
 +
older than the deeply-staining variety. In the lower part of the figure, a
 +
young corpuscle is shown.
 +
 +
Fig. 23. Compact irregular corpuscle. From a 16-cm. pig embryo. Stained
 +
with haematoxylin and Congo red. Some of the colloid is solid. No definite
 +
center is present but one is beginning to form (c s) . The nuclei are not
 +
markedly different from those of the adjacent syncytium.
 +
 +
 +
 +
THE DEVELOPMENT OF THE THYMUS.
 +
E. T. BELL.
 +
 +
 +
 +
Idn
 +
 +
 +
 +
f'^
 +
 +
 +
 +
 +
 +
 +
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 +
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 +
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 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
f
 +
 +
 +
 +
^^^ Idn
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
*-f»i
 +
 +
 +
 +
J^'\ i
 +
 +
 +
 +
1 ss
 +
 +
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 +
 +
 +
 +
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 +
 +
 +
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u
 +
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 +
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t
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
Ijlil.
 +
 +
 +
 +
 +
 +
 +
3 ?rt
 +
 +
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lb
 +
 +
 +
 +
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 +
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 +
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 +
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 +
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 +
 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
 +
 +
AMERICAN JOURNAL OF ANATOMY--VOL V.
 +
 +
 +
 +
E. T. BELL, DEL.
 +
 +
 +
 +
THE DEVELOPMENT OF THE THYMUS.
 +
E. T. BELL.
 +
 +
 +
 +
 +
12
 +
 +
 +
 +
^^®\r
 +
 +
 +
 +
 +
AMERICAN JOURNAL OF ANATOMY--VOL V.
 +
 +
 +
 +
E. T. BELL, DEL.
 +
 +
 +
 +
THE DEVELOPMENT OF THE THYMUS.
 +
E. T. BELL.
 +
 +
 +
 +
 +
rsil
 +
 +
 +
 +
rsil .,
 +
 +
 +
 +
AMERICAN JOURNAL OF ANATOMY--VOL V.
 +
 +
 +
 +
E. T. BELL, DEL.
 +
 +
 +
 +
THE YEIXS OF THE ADEE^AL.
 +
 +
BY
 +
 +
JEREMIAH S. FERGUSON, M. Sc, M. D.
 +
 +
From the Histological Laboratory of Cornell University Medical College.
 +
 +
Neio York, N. Y.
 +
 +
With 3 Text Figures.
 +
 +
Within the past decade our knowledge of the functions of the adrenal
 +
glands, and of their relations to the rest of the economy, has been greatly
 +
enhanced l)y many careful cliomical and physiological researches. Tlie
 +
recent studies of Aichel (1), Wiesel (2, 3), Soulie (4), and others have
 +
placed the early development of the organ upon a fairly certain basis.
 +
These advances in the physiology and embryology of the organ have not
 +
as yet been accompanied by corresponding advances in our appreciation
 +
of its minute anatomy. Hence this branch of the subject is, at the
 +
present time, one of unusual interest.
 +
 +
The intimate relation of the parenchyma of the adrenal to its bloodvessels, as shown by the general tendency to regard the organ as a true
 +
gland whose secretion enters its blood-vessels and leaves the organ
 +
through its efferent veins, makes it specially important that these vessels
 +
should be carefully studied and their structure and distribution accurately
 +
recorded.
 +
 +
The exhaustive study of Flint (5), on the course of the adrenal vessels,
 +
based as it was upon carefully prepared reconstructions, leaves little to
 +
be desired along this line. The writer is, however, unable to find in the
 +
literature any reference to the minute structure of the veins of the adrenal,
 +
with the notable exception of Minot's (6) article on sinusoids.
 +
 +
To be sure Pfaundler (7) mentioned the occurrence, in the medulla of
 +
the adrenal, of venous vessels whose only wall consisted of endothelium.
 +
Gottschau (8) also, though omitting their description, has figured similar
 +
vessels in his Plate XVIII, Fig. 1. But as to the structure of the larger
 +
blood-vessels of the adrenal glands the literature seems to be entirely
 +
barren.
 +
 +
The architecture of the arterial walls does not appear to offer any
 +
distinctive peculiarities, the tissues of which they consist being arranged
 +
in a manner precisely similar to that which characterizes the arteries of
 +
American Journal of Anatomy. — Vol. V.
 +
 +
 +
 +
64 The N'ein.s oi' the Adrenal
 +
 +
similar size occnrriiiii- in olliei' or<ians. 'I'lie veins, liowever, j^resent
 +
distinct and remarkable peeidiarilies which it is the iiur))()se oi' the
 +
present |>a|)ci- to describe.
 +
 +
Methods (iiid Mafcr'ud. — The tissue used for this stud}^ has included
 +
specimens of the adrenal from twenty-one human adults, together with the
 +
casual examination of fetal adrenals of the pig and of man. The
 +
adrenals of other mammals, e. g., nionke}^ dog, cat, rabbit, and guineapig, have also been more or less carefiilly studied.
 +
 +
These tissues have been fixed and hardened with many reagents, among
 +
which are Zenker's solution, formol. Miiller-formol, alcohol, corrosive
 +
acetic mixture, Tellyesniczky's fluid, and Flemming's solution. The
 +
stains used were hematein by various methods, acid hematein, iron
 +
hematein, etc., and for counter stains eosin, orange. Van Clieson's picrofuchsin, Weigert's elastic tissue stain, Mann's methyl blue-eosin mixture,
 +
Congo-red, and Ehrlich's triacid mixture. A combination of Mann's
 +
hematein, Weigert's elastic tissue stain and A'an Gieson's picro-fuchsin,
 +
gave the best results for the differentiation of the muscular and connective tissues. This method was applied as follows, and may be used
 +
after any of the above fixatives.
 +
 +
1. Stain 10-12 minutes in Mann's hematein or in Bohmer's hematoxylin, until somewhat overstained. 2. Wash well in water. 3. Stain
 +
10-20 minutes in recently prepared resorcin-fuchsin solution after the
 +
method of Weigert (9). -1. Wash in water. 5. Stain 1-3 minutes in
 +
the freshly prepared picric acid-acid fuchsin solution of A^an Gieson
 +
(10). 6. Wash and dehydrate in 95 per cent, or in absolute alcohol.
 +
7. Clear, and mount.
 +
 +
Types of Adrenal Veins. — The efferent veins of the adrenals arise in
 +
the medulla of the organ by the union of the broad capillaries of the
 +
medulla and the adjacent zone of the cortex. These capillaries form
 +
broad thin-walled vessels which have been described by Minot (6) as
 +
sinnsoids. They converge toward the middle of the medulla, where they
 +
pass into somewhat larger vessels, which, for convenience, may be termed
 +
small central veins. These veins tend toward the hilum, are relatively
 +
short, and by union with one another soon form thicker-walled vessels
 +
which may be described as large central veins. These large veins pass
 +
toward the hilum, near which, they unite to form a large efferent vessel,
 +
the suprarenal vein. This last vein makes its exit from the hilum of the
 +
organ and enters either the vena cava inferior, as is the rule on the right
 +
side, or the renal vein, as frequently occurs on the left.'
 +
 +
From this brief review of the course of these vessels it will l)e seen thai
 +
four distinct venous types have been enumerated, and it is the purpose
 +
 +
 +
 +
Jeremiah S. Ferguson 65
 +
 +
of the writer to show that these types exhibit well-defined structural
 +
peculiarities.
 +
 +
Observations. — The sinusoids, after the careful description by Minot
 +
(6), will require but brief mention. These vessels possess the wall of a
 +
capillary and the lumen of a venule. A number of such vessels may be
 +
seen in Fig. 1, in the central portion of the medulla, on either side of the
 +
group of central veins. Their wall consists of nucleated endothelial
 +
plates which rest directly upon the parenchymal cells. Their lumen is
 +
several times the diameter of the- medullary capillaries. They are dis
 +
> >
 +
 +
 +
 +
 +
 +
 +
 +
Fig. 1. A group of vessels from the central portion of tlie medulla of the
 +
human suprarenal gland, a, sinusoids; h. small central veins. Fixation, 5
 +
per cent formalin; stain, Mayer's hematein; thickness, 8// ; photomicrograph,
 +
X 100.
 +
 +
tinguished from the small central veins by the absence of connective tissue
 +
from the wall of the sinusoids.
 +
 +
The small central veins are of the type shown in Fig. 1. The wall of
 +
these vessels consists of two coats, endothelial and connective tissue. The
 +
latter is always relatively thin, though the vessels possess a very considerable lumen. Venules of this type of structure. Fig. 1, collect the blood
 +
from the sinusoids of the medulla. Frequently, however, the sinusoids
 +
open directly into the small central veins and venules, the connective
 +
tissue of the venous wall being occasionally continued for a very short
 +
distance upon the endothelium of the sinusoid.
 +
5
 +
 +
 +
 +
66
 +
 +
 +
 +
The Veins of the Adrenal
 +
 +
 +
 +
The connective tissue of the small central veins is richly supplied with
 +
elastic fibers, which are disposed in oblique nnd circular directions,
 +
 +
 +
 +
 +
Fig. 2. The medulla ol a suprarenal gland of man, showing a group of
 +
large central veins. The middle and lowermost veins are in transection, the
 +
uppermost vessel in longitudinal section. The series of sections shows that
 +
this last vessel is a branch of that in the middle of the figure. Fixation,
 +
Zenker's fluid; stain, hematein and methyl-blue, Mann's method; thickness,
 +
10 // ; photomicrograph, X GO.
 +
 +
occasional elastic fibers are also longitudinal. The typical small central
 +
veins contain no muscle. As they ap]iroach tlieir termination in the
 +
 +
 +
 +
JciTiniah S. i^\'rgiL-'on 67
 +
 +
large central veins a few smooth nnisole fibers are found, but these are
 +
always disposed in a longitudinal direction. As soon as longitudinal
 +
muscle fibers appear in apprcciahh' uiiinl)ors the venous wall acquires the
 +
type of the succeeding variety, ihc large central vein.
 +
 +
In the large central veins, as in the small, but two coats can be readily
 +
distino-uished. The inner coat, or intima, in these vessels consists of a
 +
lining endothelium, which rests upon a very thin membrane of delicate
 +
connective tissue, containing numy elastic fibers.
 +
 +
The outer coat, or adventitia, is also a vei-y thin m(!ml)rane of fibroelastic tissue, but its fibrous Ijiindlos are coarser than those of the intima,
 +
and its clastic fibers form a very close network. The outer portion of
 +
this coat contains a few longitudinal smooth muscle tibers. The great
 +
majority of these fibers, however, ai'c arranged in the form of longitudinal
 +
ridges which project into the adjacent medullary tissue. From one to live
 +
of these muscular ridges occur in the circumference of the vein (Fig. 2
 +
and 3). Except at those points at which the muscle occurs, the venous
 +
wall is extremely thin (Fig. 3). The muscular ridges are frequently so
 +
large as to materially obstruct the lumen of the vessel (e. g., the middle
 +
vessel in Fig. 2, also the uppermost vessel, which is cut in v(!ry neirly
 +
longitudinal section), and they form so noticcuible a peculiarity that
 +
their presence may be considered charactei-istic of this type of vessel.
 +
The writer has never failed to find these peculiar muscular ridges more or
 +
less highly developed in each of the human adrenals which he has examined : he believes them to be constantly present. They are less highly
 +
developed in the suprarenal vessels of the lower mammals, but even there
 +
they may frequently be demonstrated.
 +
 +
The muscle fibers of the larger ridges are an-anged in bundh^s which
 +
are enveloped in fibro-elastic septa of connective tissue. All of the
 +
muscle fibers in these bundles are longitudinally disposed. This arrangement is well shown in Fig. 3, in which a hii'ge central vein is seen in
 +
transection at a point near the entrance of a large branch. p]xamination
 +
of sections somewhat higher in the series shoAvs the union of these two
 +
vessels.
 +
 +
In the section photographed, tlu; l)raiicli has been longitudinally cut.
 +
The fine dai'k lines shown in the figure are bauds of elastic fibers which
 +
are enveloped in delicate white fibrous tissue inclosing the cut ends of
 +
the bundles of smooth muscle. The tendency to form longitudinal ridges
 +
is shown in this figure by the irregular disti-ibufion of the muscle, one
 +
side of the vessel, in both the parent stem and the branch, beirig almost
 +
devoid of muscle fibers. The muscular character' of these ridges is
 +
beyond doubt.
 +
 +
 +
 +
G8
 +
 +
 +
 +
The Veins of the Adrenal
 +
 +
 +
 +
 +
Fig 3 Large central veins from the medulla of the human suprarenal
 +
gland The figure shows the distribution of the elastic tissue and the bundles
 +
of smooth muscle which are seen in transection in the larger vein and m
 +
longitudinal section in the smaller ones below. The series shows thes.
 +
latter vessels to be branches of the former, the section being selected to
 +
show a plane near the point of division. The smaller vessels are jeiy obliquely cut and the muscle is distinctly longitudinal. Fixation Zfnker s
 +
fluid; stain, Mann's hematein, Weigert's elastic tissue, and Van Gieson s
 +
picro-fuchsin; thickness, 10 a; photomicrograph, X 37.
 +
 +
 +
 +
Jeremiah S, Eerguson 69
 +
 +
The writer has observed that the formation of such heavy ridges as
 +
those shown in Fig. 3, nearly always occurs at those points where the
 +
vessel branches. It is possible that, as in the case of the somewhat similar
 +
ridges in the veins of the erectile tissues (see Kolliker's Handbuch der
 +
Gewebelehre, 6te Aufl., 1902, pages 486 and 487), these muscular protuberances may to some extent serve the purpose of valves.
 +
 +
As the large central veins approach the hilum of the organ they form
 +
still larger vessels which partake of the structure of the suprarenal vein.
 +
The point of transition from the one type to the other is variable, occasionally the type of the large central veins is continued to the exit of the
 +
suprarenal vein at the hilum of the organ. More frequently the primary
 +
branches of the suprarenal vein may be traced for a considerable distance
 +
into the medulla of the organ, still retaining the type of structure found
 +
in the larger vessel.
 +
 +
The suprarenal vein presents three coats, intima, media, and adventitia.
 +
The tunica intima, in addition to its endothelial lining, possesses a thin
 +
membrane of very delicate connective tissue in which occasional branched
 +
connective tissue cells may be distinguished; such cells are, however, very
 +
scanty. This coat also contains a delicate network of elastic fibers.
 +
 +
The tunica media of the suprarenal vein is extremely thin, rarely ever
 +
does it exceed in thickness the tunica intima. It consists chiefly of
 +
fibro-elastic tissue, the elastic fibers forming quite a dense network.
 +
Few muscle fibers occur in this coat, nowhere are they found in sufficient
 +
numbers to form a definite layer, as in veins of similar size in other
 +
organs. Some of the muscle fibers are circularly disposed, but many of
 +
them are longitudinal.
 +
 +
The tunica adventitia is by far the thickest of. the three coats and
 +
forms two-thirds to five-sixths of the entire vascular wall. It consists
 +
chiefly of smooth muscle fibers, all of which are longitudinally disposed.
 +
These smooth muscle fibers form characteristic coarse bundles which are
 +
distributed around the entire circumference of the vessel. The largest
 +
of these bundles may occasionally form projecting ridges as in the smaller
 +
veins, but as a rule the muscular tissue is more evenly distributed than in
 +
the central veins. Each of the muscle bundles is enveloped in a perimysial sheath of connective tissue, which blends with that of the tunica
 +
media. These adventitial sheaths possess a dense network of elastic
 +
fibers, in fact the greater part of the elastic tissue in the vascular wall
 +
is frequently found in the adventitia. On its outer surface the tunica
 +
adventitia is continuous with the capsule of the adrenal or with the
 +
adjacent connective tissue.
 +
 +
This peculiar type of vessel is not strictly confined to the suprarenal
 +
 +
 +
 +
•70 'JMu' W'ins of tlie Adiviial
 +
 +
gland, but occurs, more or less typically developed, in many of the large
 +
abdominal veins, notably in the renal veins and vena cava, into which the
 +
suprarenal veins empty. But UQwhere is this peculiar venous type more
 +
strikingly developed, nowhere is the adventitia relatively so much thicker
 +
than the media, nowhere is a greater proportion of the smooth muscle of
 +
the venous wall longitudinally disposed, nowhere is there relatively less
 +
circular muscle, than in the suprarenal vein. Eealizing the intimate
 +
relation of the parenchyma of the organ to its blood-vessels, and adopting, if we may, the accepted physiological function of the adrenal — the
 +
formation of an internal secretion, a powerful vaso-constrictor which is
 +
poured into the blood within the capillaries and veins of the organ — ^the
 +
peculiar longitudinal arrangement of the muscular tissue, the valve-like
 +
protuberances at the junctions of the venous vessels, the absence of circular
 +
muscle from the walls of the veins of all sizes, and the general appearance
 +
of these vessels which are so remarkably different from the veins of most
 +
other organs, become, to say the least, extremely significant of a close
 +
structural relation, physiologically speaking, to the presence of an
 +
astringent secretion in the outflowing blood current.
 +
 +
In this connection, one further observation is of importance. In the
 +
periadrenal connective tissue are numbers of small veins which return the
 +
abundant blood supply of the tissues of this region, most of them
 +
emptying into the phrenic veins. Many of these veins do not differ from
 +
the similar veins of other parts, but in many others the writer has observed that the muscle tissue is almost entirely disposed in a longitudinal
 +
direction, a condition which is quite the reverse of that found in the
 +
adipose and areolar tissues of other portions of the body.
 +
 +
The writer also finds that many of the small veins of the adrenal,
 +
instead of opening into the central veins as is usually the case, pursue a
 +
less frequent course, penetrating the cortex and capsule of the organ, and
 +
emptying into the small veins of the surrounding connective tissue. The
 +
frequency with which this condition was associated with the occurrence
 +
of longitudinal muscle fibers in the periadrenal veins, suggests a more
 +
than casual relationship between the two conditions.
 +
 +
Summary.
 +
 +
In conclusion, the above facts may be summarized as follows:
 +
 +
1. The efferent blood-vessels of the adrenals form four successive vascular types, the sinusoids, the small central vein, the large central vein,
 +
and the suprarenal vein.
 +
 +
2. Each of these types presents distinctive characteristics.
 +
 +
 +
 +
Jeremiah S. Ferguson 71
 +
 +
3. In all four tA^pes circular muscle is either absent or noticably
 +
deficient.
 +
 +
4. In the large central veins prominent and characteristic muscular
 +
ridges are constantly present, and are frequently in relation with those
 +
points at which the branches of these vessels enter.
 +
 +
5. These peculiarities of structure may possibly bear a close physiological relation to the function of the adrenal as a gland that forms an
 +
internal secretion which has been shown to be a powerful vaso-constrictor
 +
and stimulant of smooth or involuntary muscle.
 +
 +
BIBLIOGRAPHY.
 +
 +
1. AicHEL.— Munch, med. Wochenschr., 1900, XLVII, 1228; and Arch. f. mik.
 +
 +
Anat, 1900, LVI, 1.
 +
 +
2. WiESEL.— Anat. Hefte. 1901, XVI, 115.
 +
 +
3. Ibid., 1902, XIX, 481.
 +
 +
4. SouLiE.— J. de I'anat. et de la physiol., 1903, XXXIX, 197, 390, 634.
 +
 +
5. Flint. — Contrib. -dedicated to W. H. Welch, Baltimore, 1900, 153; also in
 +
 +
Johns Hop. Hosp. Rep., 1900, IX, 153.
 +
 +
6. MiNOT.— Proc. Post. Soc. Nat. Hist., 1900, XXIX, 185.
 +
 +
7. Pfaundler. — Sitz. d. Akad. d. Wissensch., Wien, 1892, CI, 515.
 +
 +
8. GoTTSCHAU.— Arch. f. Anat., 1883, 412.
 +
 +
9. Weigert.— Centralbl. f. allg. Path. u. path. Anat., 1898, IX, 289.
 +
10. Freeborn.— Proc. N. Y. Path. Soc, 1893, 73.
 +
 +
 +
 +
THE BLOOD VESSELS OF THE PEOSTATE GLAND.
 +
 +
BY
 +
 +
GEORGE WALKER, M. D.
 +
 +
Associate m Surgery, Johns Hopkins University.
 +
From the Anatomical Laboratory, Johns Hopkins University.
 +
With 2 Colored Plates.
 +
 +
As the structures of the body are being more and more carefully investigated it is found that organs are composed of like structural units,
 +
which when repeated a number of times form the whole organ. In general these units are formed by the glandular structures, the blood-vessels, or by both, as is the case in the liver.
 +
 +
Some eight years ago, at the suggestion of Dr. Mall, I undertook the
 +
study of the prostate gland, with the hope of finding structural units in
 +
it. In this search I was successful. Since then my work has been
 +
continued in the laboratories of Professor Born ^ of Breslau and Professor Spalteholz ^ of Leipzig, and although this communication is several years late in appearing, it should in reality have preceded those that
 +
were published in 1899.
 +
 +
In the present study for the most part the prostate glands of dogs were
 +
used. Several cadavers were injected and the gross blood supply was
 +
studied in part from these. After the animals had been killed by chloroform, the aorta was exposed just above the bifurcation and injected with
 +
various substances. A preliminary washing out of the blood-vessels with
 +
salt solution was practised in a few of the first injections, but this was
 +
soon discarded as it did not seem to enhance the value of the method.
 +
 +
Carmine gelatine, followed by ultramarine-blue gelatine, as an injecting
 +
mass, gave the most satisfactory results. About 250 cc. of the carmine
 +
gelatine were injected first, the injection being stopped as soon as all
 +
of the tissues had acquired a maximum carmine hue. This was followed by the injection of ultramarine-blue gelatine, which was kept up
 +
until no more of the material would pass in. The carmine gelatine
 +
 +
^Walker. George: Ueber die Lymphgefasse der Prostata beim Hunde.
 +
Arch, fiir Anatomie, 1899.
 +
 +
= Walker, George: Beitrag zur Kenntnlss der Anatomie und Physiologie
 +
der Prostata, etc. Ibid., 1899.
 +
American Joukxal of Anatomy. — Vor-. V.
 +
 +
 +
 +
74 Tlif lUood Vessels of the Prostate Cilaiicl
 +
 +
filled the arteries, capillaries, and veins ; the blue passed into the arteries
 +
and arterioles, displacing the gelatine and filling them, but was stopped
 +
at the capillaries because the nltramarine-blue granules were too large
 +
to enter them. In a specimen thus prepared the arteries appear blue, and
 +
the capillaries and veins red. This is shown in Figure 1, with colors
 +
reversed, in order to present the conventional appearance. As it was
 +
impossible to get a perfectly complete injection in one specimen, several
 +
of the best were selected and the gaps filled in, with the results as shown
 +
in Figure 1. One section, however, is remarkably beautiful and presents
 +
a picture very closely resembling that seen in this figure.
 +
 +
In order to map out the complete network of arteries surrounding a
 +
separate lobule, I injected them with Prussian blue, then opened the
 +
urethra, and injected carmine gelatine into a prostatic duct through a
 +
very fine blunt hypodermic needle. A specimen made in this way is shown
 +
in Figure 2 where the ducts are represented in brown. The capillaries
 +
were studied in a specimen which had been completely injected with carmine gelatine. A very thin section of this was stained with iron hematoxylin, and is shown in Figure 3. The basement membrane is artificially tinted with yellow so as to make it visible.
 +
 +
The technique of the injecting is rendered difficult by the fact that the
 +
situation of the gland in the pelvis is somewhat remote. In all, about
 +
 +
75 dogs were used before a complete circulation cycle could be seen.
 +
Cinnabar, lampblack, and various other substances were tried, but they
 +
did not prove as good as the combination of carmine gelatine followed by
 +
ultramarine-blue gelatine.
 +
 +
When the ordinary directions for preparing carmine gelatine were followed, it always proved difficult to get a perfectly transparent substance.
 +
The trouble is connected with the neutralization of the ammonia by the
 +
acetic acid. The gelatine should be rendered practically neutral, but
 +
if the reaction is carried the least bit too far, the solution becomes
 +
cloudy. Sometimes two drops of the acetic acid are sufficient to make
 +
turbid a whole litre of the prepared carmine. After a good many
 +
trials, the following method was adopted : Take 10 cc. of the ordinary laboratory ammonia and dilute with 40 cc. of distilled water,
 +
then determine by titration the exact amount of the laboratory acetic
 +
acid which will neutralize it. After this determination has been made,
 +
10 grms. of pure carmine are rubbed up with 50 cc. of distilled water;
 +
then 25 cc. of the ordinarj^ ammonia are measured, and a few drops at a
 +
time are poured into the carmine mixture which is kept constantly rubbed
 +
up. This process is very closely watched, and the ammonia is gradually
 +
added until the carmine is completely dissolved, and the mixture becomes
 +
 +
 +
 +
George Walker 75
 +
 +
translucent and assumes .a dark red color. Tlie amount of ammonia
 +
used is determined by referring to the vessel in which the 25 cc. have been
 +
measured. The gelatine in whatever proportion it is required — according
 +
as a thin or thick solution is desired — is dissolved in the distilled water,
 +
and the carmine solution is added to it. We then calculate how mucli
 +
acetic acid will be required for the amount of ammonia w^hich has been
 +
used; this is measured and added, drop l)y drop, to the mixture which is
 +
constantly stirred. A sufficient quantity of water is then added to bring
 +
the amount up to a litre. I found that in this way I could always obtain
 +
a beautifully clear gelatine and was never annoyod by the failures and
 +
uncertainties belonging to tlie other method.
 +
 +
Akteries. .
 +
 +
The prostate gland derives its arterial supply from the internal iliac
 +
arteries by means of four branches; the superior vesical, the inferior
 +
vesical, a small branch from the inferior hemorrhoidal, and a small terminal branch from the internal pudic artery. These vessels will be found
 +
illustrated in my paper published in 1899. The superior vesical, a
 +
branch from the internal iliac, which is given off high up, divides before
 +
reaching the bladder, into two fair-sized branches ; the lower and smaller
 +
branch extends downward and supplies the vesical third of the prostate ;
 +
this branch is sometimes called the middle vesical artery. The inferior
 +
vesical, which is a large branch, is practically the main blood vessel of the
 +
prostate gland, and should be called the prostatic artery for, in the
 +
majority of instances, it does not send any branches to the bladder. The
 +
major part of the gland is supplied by this vessel; it courses along the
 +
vesicorectal fascia and meets the prostate at its lower border, where it
 +
usually divides into seven branches, four of these enveloping the anterior,
 +
and three the posterior surface. The posterior are about one-half the
 +
size of the anterior lu'anchcs. These vessels are situated in the capsule of
 +
the gland and envelop it as the fingers of one's hand would do in clasping
 +
a round object. From these trunks a numljer of smaller ones are given
 +
off, so that a very close arterial network is formed over the surface of the
 +
gland. The ])ranch from the inferior liemorrhoidal is not constant; in
 +
fact, it appears to be more often absent than present. When it is seen,
 +
it occurs as one or two small branches wbicli ]uQci llic prostate in its
 +
urethral half, and extend over the surface as line vessels which anastomose
 +
with the vesica] artery. The internal ])iulic branch is fairly constant.
 +
It extends along Ibc membranous urotlira and ])lunges directly into the
 +
prostatic substance usually without giving off any l)ranclies to the surface.
 +
 +
 +
 +
7G The Blood Vessels of the Prostate Gland
 +
 +
A slight anastomosis is occasionally seen. The vessels supplying the two
 +
sides of the gland are distinct. The only anastomosis across the median
 +
line is by way of the venous channels around the urethra.
 +
 +
From the large superficial branches above described, smaller ones are
 +
given off at right angles, and pierce the gland in places corresponding to
 +
the divisions of the lobules (Art. Fig. 1). Here they penetrate the
 +
fibrous-tissue septa, and extend to the urethra, becoming smaller and
 +
smaller, however, as they approach it, so that in this region they are seen
 +
as very delicate terminal vessels. As they pass down, they give off
 +
branches which penetrate into the lobule and finally divide into myriads
 +
of capillaries which pass around the alveoli, and come in very close
 +
relationship with the secreting cells. From these cells they are separated
 +
simply by a delicate basement membrane composed of fine fibrils. From
 +
the superficial vessels branches are given off which enter the lobule directly, that is, they do not pass first into the fibrous-tissue septa {8up. Br.
 +
Fig. 1). On the anterior surface there are usually two branches which
 +
do not give off as many smaller ones as the rest, and consequently remain
 +
larger and extend over to the middle line, where they dip into the median
 +
fissure and supply the median side of the lobules (Med. Br. Fig. 1).
 +
 +
The arrangement on the posterior surface corresponds to that seen on
 +
the anterior surface, in so far as the supply of the lobules is concerned.
 +
On the posterior surface toward the bladder one vessel penetrates the substance of the gland and runs directly to the caput gallinaceum (Art. Col.
 +
Sem. Fig. 1). Here it divides into a fine network and supplies the erectile tissue of the organ. Before this vessel reaches the eminence a small
 +
trunk is given off which extends to the ejaculatory duct (Ai-t. duct. ej.
 +
Fig. 1). The branch supplying the caput gallinaceum is usually derived
 +
from the pudic; sometimes it comes from the inferior vesical.
 +
 +
The arterial supply in the connective tissue toward the urethra is much
 +
poorer than in the secreting portion. Here the vessels terminate in fine
 +
branches, relatively somewhat sparsely scattered. The arterial arrangement is shown on the red side of Figure 1.
 +
 +
Capillaries.
 +
 +
The capillaries form a very complete and elaborate network around the
 +
alveoli of the lobule. Here, as is seen in Figure 3, they surround an
 +
alveolus in a more or less circular manner, and upon these vessels the
 +
cells rest almost directly, being separated only by the very delicate connective-tissue basement membrane. From this outside capillary, a folding in is seen, which forms a definite loop (Cap. L. Fig. 3.) This at
 +
 +
 +
 +
George Walker 77
 +
 +
first sight might appear to end blindly, but a more careful study reveals
 +
the two branches, which sometimes appear winding around each otlier,
 +
and presenting enlarged club-shaped ends. The cells rest on these as
 +
they do on the circular portion. Under the low power, the epithelial cells
 +
appear to be in direct contact with the capillaries, and it is only by the
 +
aid of the oil immersion that a very delicate connective-tissue basement
 +
membrane is seen. This is shown artificially colored as B. M. Fig, 3.
 +
This membrane contains a few elastic fibers.
 +
 +
Veins.
 +
 +
On the surface of the gland are veins corresponding to the arteries
 +
which lie in the capsule. As a rule they merge into two main trunks
 +
corresponding to the vesical arteries; occasionally several small branches
 +
pass off into the middle hemorrhoidal vein.
 +
 +
The superficial veins do not drain the blood from the whole gland, but
 +
only from the outer fourth, as is shown in Fig. 1. From the inner threefourths of the gland the blood passes towards the centre, and into the
 +
large venous sinuses which are a continuation of the corpora spongiosa.
 +
(Co. 8p. Fig. 1). These immediately surround the urethra. The large
 +
venous trunks which collect the blood from the gland do not lie on the
 +
same plane as the arteries, but are situated in the fibrous septa some
 +
little distance removed from them. These run, as do the arteries, on the
 +
outside of the lobule, and are interlobular, not intralobular. For the
 +
venous return from the caput gallinaceum there is no distinct vessel corresponding with the artery, but there are anastemoses with the spongy
 +
plexus.
 +
 +
The venous plexus around the urethra is, as before stated, a continuation of the corpora spongiosa. The blood from this region passes away
 +
into the internal pudic vein. Occasionally two or three small veins
 +
drain the tissues from this region, pass out of the prostate and run along
 +
the membranous urethra and off into the vesicorectal fascia.
 +
 +
There is an anastomosis of the veins in the prostate and bladder where
 +
these organs come together, and also on the outside through the superior
 +
vesical veins. There is, of course, an anastomosis of the urethral veins
 +
through the corpora spongiosa plexus.
 +
 +
SUMMAEY.
 +
 +
The prostate gland is supplied with blood by branches of the internal
 +
iliac arteries, viz., the superior vesicals, inferior vesicals, inferior hemorrlioidals, and internal pudics; the main blood supply comes from the
 +
inferior vesicals.
 +
 +
 +
 +
78 The Jilood Vessels of I lie Tr. .slate 11 land
 +
 +
Branelies of llieso envelop iho siirfaec! of tlie <ilnii(l and f^ivo ofT smallcMon(>s, wliieli iieiielratc between tlie lohnles in (he fihrons-tissuc septa.
 +
 +
The caiiilhii-ies are separated iVom I he ciiilhclial cells only by a very
 +
lliiii haseiiieiil iiieiiibraiie.
 +
 +
'I'here are siipei-llcial vcins correspond iiiii' with the arteries.
 +
 +
I'\)i- the Older superficial fourth of the gland the return flow is towards
 +
(lie siirfaei'. 'I'he inner three-fourths are drained by veins wliieb enijjiy
 +
into the venous plexus immediately aronnd the nretlira.
 +
 +
The lobule is formed primarily by the individual gland dnets as shown
 +
in Figure 2. The main arteries surround this lobnle wliicb they penetrate at many points. The veins leave the lobule mainly at its peripheral
 +
5ind central (urethral) ends as shown in Fig. ].
 +
 +
EXPLANATION OF PLATP^S I AND II.
 +
 +
Fid. 1 is Irom a section of a prostate gland of a dog injected with carmine
 +
gelatine and ultramarine-blue gelatine. The arteries in the section were
 +
blue, the veins and capillaries red. The section was cut free hand, about
 +
r.O// in thickness, and cleared both in glycerine and in creosote. In the
 +
figure this artery is red and this vein blue. Ar^., Arteries; Art. Col. Ficm..
 +
Artery of the colliculus seminal is; Art. duct, cj.. Artery of the ejeculatory
 +
duct; Col. Scni., Colliculus s(>niinalis; V. J'L, Venous plexus around the
 +
urethra.
 +
 +
Fio. 2. Lobule of prostate from a gland which had been injected with
 +
uHramarine-golatine blue into the artery, and with carmine gelatine into
 +
the prostatic duct. Pr. duct., Opening of the prostatic duct into the urethra;
 +
Gl. Tis., Gland tissues distended with carmine gelatine; Art., Surrounding
 +
artery. In this figure the artery is represented in red and the ducts in brown.
 +
 +
Fig. 3. Very thin section from Ihe i)rostate gland of a dog. Capillaries in
 +
red, injected with carmine gelatine. Section stained with iron h;rmatoxylin,
 +
with artificial yellow tinting of basement membrane. Oil immersion with
 +
one inch eye-piece amplification. Cap., Capillaries; B. M., Basement membrane; 07. Ep., Glandular epithelium; Cap. L., Capillary loop.
 +
 +
 +
 +
BLOOD VESSELS OF PROSTATE GLAND
 +
 +
GEORGE WALKER
 +
 +
 +
 +
PLATE
 +
 +
 +
 +
 +
a)
 +
CO
 +
 +
 +
 +
Q
 +
 +
 +
 +
AMERICAN JOURNAL OF ANATOMY — VOL. V
 +
 +
 +
 +
BLOOD VESSELS OF PROSTATE GLAND
 +
 +
GEORGE WALKER
 +
 +
 +
 +
PLATE II
 +
 +
 +
 +
GI.Tis.
 +
 +
 +
 +
Art
 +
 +
 +
 +
 +
PKDuct
 +
 +
Fig. 2
 +
 +
 +
 +
 +
Cq1>.L,
 +
 +
 +
 +
NCap.
 +
 +
 +
 +
Krcj. 3
 +
 +
 +
 +
AMERICAN JOURNAL OF AN ATOM Y--VO L. V
 +
 +
 +
 +
THE EMBKYONIC DEVELOPMENT OP THE EETE-CORDS
 +
AND SEX-COEDS OF CHRYSEMYS.
 +
 +
BY
 +
 +
BENNET M. ALLEN,
 +
 +
Instructor in Yertehrate Anatomy, University of Wisconsin.
 +
 +
With 1 Double Plate akd 6 Text Figures.
 +
 +
A glance at the diagrams on the next page will at once serve to show the
 +
great difference of opinion that has prevailed in regard to the origin of
 +
the sex-cords and rete-cords of the Sauropsida. In fact, it is hard to
 +
conceive of any possible manner of origin that has not been held to be
 +
correct by some well-known embryologist.
 +
 +
The Chelonia have remained almost untouched in the study of this
 +
problem. Only one work has appeared upon the rete-cords (Von Moller,
 +
98), while no work has been published upon the subject of the sex-cords.
 +
 +
Von ]\Ioller studied two turtles, one a specimen of Emys lutaria of
 +
2.5 cm. plastron length, and the other Clemmys leprosa of 4.9 cm.
 +
plastron length. He observed no connection between the testis and
 +
Wolffian body. This caused him to remark :
 +
 +
" Dieser Befund wird hochst auffallig, wenn mann bedenkt dass die
 +
Beobachtungen an Amphibien zeigen dass die Verbindungen zwischen
 +
Hoden und Wolffschen Gauge schon dann angelegt und vollendet werden,
 +
wenn die iibrigen Organe sich noch in der Entwickelung befinden, und
 +
wenn das Junge in der Eischale, respective in Uterus eingeschlossen ist.
 +
Die zwei von mir untersuchten Schildkroten hatten dagegen schon seit
 +
Monaten die Eischale verloren, und doch war bei ihnen noch kein einzige
 +
Verbindung zwischen Hoden und Wolffschen Gauge vorhanden, obwohl
 +
Anlagen dieser Verbindungen sich bereits vorfanden."
 +
 +
It is quite unfortunate that he considered these stages to be early
 +
enough for his purpose, since my work has shown the rete-cords to be
 +
formed at a relatively early stage of development in Chrysemys.
 +
 +
Von Moller sums up his results as follows : " Ich finde also bei diesem
 +
Thiere zwischen Hoden und Wolffschen Korper noch keine Verbindungen, dagegen im Mesorchium und im oberflachlichen Bindegewebe der
 +
Umiere solide Zellenstrange, fiir welche ich genotigt bin einen UrAmeeican Journal op Anatomy. — Vol. V.
 +
 +
 +
 +
80
 +
 +
 +
 +
The Eete-Cords and Sex-Cords of Chrysemys
 +
 +
 +
 +
Text Fig. A.
 +
 +
 +
 +
 +
GER
 +
 +
 +
 +
Text Figs. A-D. Diagrams ilhistrating various views held by authors
 +
whose Avritings are reviewed in this
 +
article.
 +
 +
^D.— Fundament of the adrenal
 +
body.
 +
GER.— Germinal epithelium.
 +
 +
il/.— Mesentery.
 +
M. P.— Malp'ghian corpuscle.
 +
J?.— Rete-cord.
 +
S. C.-Sex-cord.
 +
U. r.— TJriniferous tubule.
 +
W. I).— Wolffian duct.
 +
 +
 +
 +
spningsort anzunehmen, der wedcr in
 +
den Geweben der Urniere noch in denen
 +
dcs Hodens liegt denn ieh woder im
 +
Stande bin einen Ziisammenhang mit
 +
den gewundenen Kanalchen des Hodens
 +
nachznweisen, noch einem solchen mit
 +
deni Epithel Bowmanscher Kapseln
 +
oder sonst mit Theilen der Urniere.
 +
Ich nehme daher an, dass sie vom Peritoneum stammen." No further allusion need be made to this article.
 +
 +
Turning to the other groups of the
 +
Sauropsida, we find a large mass of literature. To intelligently discuss this,
 +
we must use precise terms. The sexcords are those masses or cords of cells
 +
which eventually become the seminiferous tubules of the testis or the medullary cords of the ovary. The rete-cords
 +
are those structures which eventually
 +
give rise to the canals which unite the
 +
seminiferous tubules or medullary cords
 +
of the sex-glands with the ducts of the
 +
mesonephros.
 +
 +
It is not necessary to enter into a
 +
lengthy review of the literature upon
 +
this subject. That has been thoroughly
 +
done by Born, 94, MihalkovicS;, 85,
 +
Janosik, 85, Coert, 98, Winiwarter, 00,
 +
and others. A few diagrams will suffice to show, in a sufficiently vivid manner, the wide differences between the
 +
many views upon this subject as expressed in the papers most worthy of
 +
note.
 +
 +
The names associated with the different diagrams, Text Figures A-D. are
 +
those of the authors who have held
 +
views represented by the diagrams so
 +
indicated. After the name of each
 +
 +
 +
 +
Bennet M. Allen 81
 +
 +
author are placed the names of the forms which he studied in arriving
 +
at his conclusions.
 +
 +
A. Tubules arise from the Wolffian duct and grow into the sex-gland
 +
fundament. Their distal portions form the sex-cords while their proximal portions form the rete-tubules.
 +
 +
70, Waldeyer — Chick (Gallus).
 +
 +
B. According to this view, evaginations grow out from the capsule
 +
of Bowman. Distal branches from these stems pass down into the sexgland fundament to form sex-cords, while the more proximal portions of
 +
the evaginations remain attached to the capsules of Bowman and serve
 +
as rete-tubules.
 +
 +
Brann, 77, Platydactylus, Tropidonotus.
 +
 +
Weldon, 85, Lacerta.
 +
 +
Hoffmann, 8g and 92, Lacerta, Hjematopsis, Sterna, Gallinula.
 +
 +
Semon. 87, Gallus.
 +
 +
Peter, 04, Lacerta.
 +
Brann, 77, considers the rete-sex-cords to be, in the strictest sense,
 +
segmental in arrangement. He expressly denies that the cells that contribute to the formation of the adrenal body are derived from branches
 +
of the evaginations from the capsules of Bowman, as asserted by Weldon,
 +
85 and Hoffmann. 89 and 92. These two last named authors asserted
 +
that each evagination divides into a dorsal and a ventral branch, the
 +
former suppljdng the cells of the cortical portion of the adrenal body,
 +
and the latter forming the sex-cords. Semon, 87, was not so clear upon
 +
the question. He merely stated that the anastomosing cords arising from
 +
the capsule of Bowman pass into the adrenal and sex-gland fundaments,
 +
— the more dorsal to the former, the more ventral to the latter.
 +
 +
C. Large numbers of cells migrate from the germinal epithelium into
 +
the underlying stroma. From this unorganized blastema, the sex-cords
 +
are formed, suddenly crystallized as it were. The rete-cords are formed
 +
of evaginations from the capsule of Bowman.
 +
 +
Schmiegelow, 82, Gallus.
 +
Mihalkovics, 85, Lacerta, Gallus.
 +
Laulanie, 86, Gallus.
 +
 +
D. According to Janosik, the sex-cords arise as direct ingrowths from
 +
the germinal epithelium. Cords of cells grow from their distal ends to
 +
the capsules of Bowman, thus forming the rete-cords. Cords of cells
 +
grow in from the peritoneum between the sex-gland fundament and the
 +
mesentery to form the cortical portion of the adrenal body.
 +
 +
Janosik. 90, Gallus. .
 +
 +
6
 +
 +
 +
 +
83 The Kete-Cords and Sex-Cords of Chrysemys
 +
 +
The following table will show the great difference of opinion held by
 +
authors working upon the same identical species. The view held is indicated in the same manner as above.
 +
 +
Lacerta agilis — Weldon (B) ; Hoffmann (B) ; Mihalkovics (C).
 +
Chick (Gallus)— Waldeyer (A); Semon (B) ; Mihalkovics (C) ;
 +
Laulanie (C) ; Schmiegelow (C) ; Janosik (D) ; Weldon (?),
 +
 +
We cannot close an account of the literature upon the subject without
 +
refeiring to the work of Semon, 91, upon Ichthyophis, one of the Gymnophiona, and a paper by Semper, 75, upon the Sex-glands of the
 +
Elasmobranchs.
 +
 +
Semon^ 91, considers the nephrotome to be the ventral portion of the
 +
mesoblastic somite. This view, by the way, is also held by Brauer, 02.
 +
Semon states that after the nephrotome breaks away from the myotome
 +
and sclerotome, it still remains attached to the peritoneum (unsegmented
 +
mesoderm) by means of two bridges of cells — a lateral and a medial.
 +
The major part of each nephrotome forms a Malpighian corpuscle of the
 +
mesonephros. The lateral of the two bridges connecting it with the
 +
peritoneum becomes its peritoneal funnel (nephrostome), while the
 +
medial bridge sends out a process which divides into a dorsal branch passing to the adrenal body, and irre|gular branches (sex-cords) non-segmental in character, that pass to the sex-glands, there to come in
 +
contact, in the case of the male, with the seminal vesicles, which, are
 +
derived from the germinal epithelium. He holds a theory that the
 +
pronephros extends in rudiment, at least, along the entire length of the
 +
mesonephros, and that this pronephric rudiment develops into the
 +
adrenal body. He considers the dorsal branches spoken of above, to be
 +
these vestiges of the pronephros.
 +
 +
Semper, 75, gives the most interesting account of the rete in the male
 +
of Acanthias. According to him, each of the 34 primary Malpighian
 +
corpuscles of the kidney is connected with the body cavity by a peritoneal
 +
funnel. Seven of the most anterior of these funnels lose their union
 +
with the peritoneum and take on the form of vesicles. Three or four
 +
of them now fuse together to form the " central canal," which lies at the
 +
base of the testis and parallel with it. From this central canal there arise
 +
a number of irregular anastomosing canals which extend into the testis
 +
and come in contact with the true sex-structures (Vorkeimketten) that
 +
have arisen from the germinal epithelium. This net-Avork of rete-cords
 +
he calls the rete-vasculosum.
 +
 +
In other forms there exists a somewhat modified condition of considerable interest. In comparing Acanthias and Mustelus, Semper said:
 +
" Trotzdem scheint ein grosser Untershied in Bezug auf die Entstehung
 +
 +
 +
 +
Bonnet j\r. Allen 83
 +
 +
des Centralcanals des Ilodens /wisclien ]\[usleliis ;ind Acantliias zu bestehen. Bei dieser Gattung wird or seiner ganzer Liingc nach gebiklet durcli
 +
die Verwachsimg der seitlicli vom Segmantalgang nach vorn sich wendenden Trichterblasen. Seitliche Aiisbuchtiingen der letztoren bildeii den
 +
basalem Theil der rete vascolosum. Bei Mnstehis dagegen ist es nur der
 +
vorderste iiber die Hodenfalte hinaiis vorgreifende Abschnitt des Centralcanals den mann entstanden ansebcn konnte, denn nur an diesen setzen
 +
sich 2 (oder 3) Segmentalgange an. Der ganze iibrige viel langere
 +
Theil des Centralcanals entsteht aiis den in das Stroma der Epithelfalte
 +
eingestiilpten Keimepithel Zellen."
 +
 +
Balfour, 78, shows that in the forms which he studied, the anterior
 +
end of the sex-gland only, was directly united to the mesonephros by
 +
means of the rete-canals.
 +
 +
The condition in the lizard Platydactylus is, according to Braun, 77,
 +
quite similar. He considers the union to be formed in adult life by two
 +
or three rete-cords joining the anterior ends of mesonephros and testis;
 +
although he states that they are connected along the entire length of the
 +
testis in early stages. Ploffmann, 89, finds the union of rete-cords to be
 +
complete and intact along the entire length of the testis in Lacerta at the
 +
end of the first year. He did not study older specimens. Semon, 87,
 +
claims that there is a degeneration of the rete-cords at both the anterior
 +
and posterior ends of the sex-gland of the chick; but Janosik, go, denies
 +
this.
 +
 +
Material axd Technique.
 +
 +
Our lakes in the vicinity of Madison abound in the little painted
 +
tortoise, Chrysemys marginata. The number of embryos to be gathered
 +
in the season is limited only by ones patience in the work of preserving
 +
them. I have prepared a large number of serial sections of the mesonephros and sex-gland, as well as of entire embryos, comprising an unbroken chain of stages from gastrulation to adult life.
 +
 +
As a fixative, TQllyesnitzky's Bichromate-acetic fluid was almost exclusively used, as it gave most excellent results. Haidenhein's iron-ahnn
 +
hsematoxylin stain proved unsatisfactory for early stages of the embryos
 +
under 7 mm. length. For later stages than this it gave excellent results
 +
and was used almost exclusively. A counter-stain of Congo red was also
 +
employed. The sections were cut at a thickness of 7 fi.
 +
 +
Measurements Avere made of the distance between the cervical bend
 +
and the tail bend (C-T). In the later stages the length of carapace was
 +
also given.
 +
 +
To more clearly understand the origin of the sex-cords, it will be
 +
 +
 +
 +
84
 +
 +
 +
 +
The Eete-C'ords and 8ex-Cords of Chrysemys
 +
 +
 +
 +
necessary to first understand certain features in the development of the
 +
niesonephros. Reference to these features will be made only in so far as
 +
they concern the subject of this paper. In an early stage of development (C-T. 3.5 mm.), a section through the posterior part of the sexgland fundament shows the mesoblastic somites to be attached to the
 +
lateral plates by the unmodified middle plate (Text Figure E). The
 +
cells of the latter are arranged in two rows, in such a manner as to leave
 +
a line of weakness between, which may be considered as a rudimentary
 +
lumen, connecting the body-cavity on the one hand with the cavity of the
 +
 +
 +
 +
 +
^'iEF
 +
 +
 +
 +
Text Fig. E. Transverse section through the middle of the mesonephros
 +
fundament of an embryo of 3.5 mm. C-T. length.
 +
 +
AO. — Aorta. NO. — Notochord.
 +
 +
EC. — Ectoderm. SO. — Somatopleiire.
 +
 +
MY. — Myotome. 8P. — Splanchnopleure.
 +
 +
N. — Neural canal. WD.— Wolffian duct.
 +
 +
NEP. — Nephrotome.
 +
 +
 +
 +
mesoblastic somite on the other. In the region posterior to this, these
 +
relations become even more marked.
 +
 +
More anteriorly, just behind the interesting region which forms a
 +
transition between the pronephros and mesonephros, the middle plate is
 +
found to be wholly broken away from the mesoblastic somites, and to be
 +
divided by transverse intervals into nephrotomes which occur in the
 +
number of three to four per somite.
 +
 +
I found no evidence of a primary metamerism of these nephrotomes.
 +
So soon as the middle piece appeared to be broken up at all, the number
 +
of nephrotomes here recorded appeared. Special investigation along this
 +
 +
 +
 +
Bennet M. Allen 85
 +
 +
line, however, might show a primary metamerism, from which the above
 +
described condition was derived by further secondary splitting of the
 +
nephrogenous tissue. Each nephrotome becomes vesicular to within a
 +
sliort distance of the peritoneum thus forming the primary Malpighian
 +
corpuscles. The remaining portion of the nephrotome uniting it with
 +
the peritoneum becomes, in later stages, the peritoneal funnel or nephrostome, while the uriniferous tubule arises as an outgrowth from the distal
 +
end of the nephrotome. The mesonephric peritoneal funnels are vestigial
 +
structures from the time of their origin.
 +
 +
In later stages {C-T. 6 mm.), two sharply defined regions of the
 +
mesonephros may be distinguished from one another. In the anterior
 +
part of the sex-gland, only the primary Malpighian corpuscles are
 +
formed. Each is well developed, the glomerular invagination having
 +
already taken place. The 11th to 21st Malpighian corpuscles are connected with the peritoneum by peritoneal funnels (Plate I, Fig. 5), some
 +
of which are much better developed than others, there being great variation among them. In the best developed among them, the end attached
 +
to the peritoneum flares open to form an actual funnel-like mouth, yet
 +
this opening is never continuous with that of the Malpighian corpuscles.
 +
The greater part of the peritoneal funnel is merely a cord of cells. In
 +
some cases even, it has lost its continuity with the capsule of Bowman.
 +
At this stage the first ten Malpighian corpuscles are without peritoneal
 +
funnels.
 +
 +
Caudad of the 21st Malpighian corpuscle, each nephrotome shows two
 +
or three rudimentary vesicular enlargements. Each enlargement is destined to form a Malpighian corpuscle. The most ventral of these we
 +
shall consider as the primary Malpighian corpuscle. It is still rather
 +
broadly connected with the peritoneum. This place of union we shall
 +
consider as a rudimentary peritoneal funnel, although it has no flaring
 +
opening.
 +
 +
In later stages, secondary and tertiary Malpighian corpuscles appear
 +
in the anterior region described above, thus making the total number per
 +
somite approximately equal to that in the posterior region. Eoughly
 +
speaking, from nine to twelve Malpighian corpuscles in all, appear in
 +
each somite.
 +
 +
Eeference to Plate I, Fig. 1, will show certain of the points mentioned
 +
above. Furthermore, one can see an elongated mass of tissue that extends from each peritoneal funnel dorso-mediad and which lies just laterad of the V. renalis revehens (vena cava). This we shall term the funnel-cord. They appear in both the anterior and posterior regions of the
 +
mesonephros as described above and are co-extensive with the sex-gland
 +
 +
 +
 +
86 'I'lic li'i'ti'-('<)i-(ls and Sex-Cords of Chrysemys
 +
 +
I'liiidaiiiciit. in fact (licy ai'c found foi' a slioi't distance anterior to it
 +
Naturally each I'unnel-iord lies ()|)])ositc a ])i'iniarv Malpit^'liiaii eorpnscle,
 +
and likewise to the series of secondary, tertiary, etc., corpuscles formed in
 +
a vci'tical row above it. Each cord is made np of rather loosely arranged
 +
cells that bear a rather close resemblance to the mesenchyme cells. In
 +
fact the nuclei of these cells, the funnel cells, and the cells of the" peritoneum are not to be distinguished from one another. Cytoplasmic differences alone appear and these depend upon the density of the tissue. In
 +
some cases a slight evagination of the capsule of Bowman is found at
 +
the point where it joins the peritoneal funnel. This evagination may
 +
take various forms and in many cases is -wholly absent. Such an appearance may have led to the view held by some authors that these cords
 +
arise as outgrowths from the capsules of Bowman. This view would be
 +
still further justified if the peritoneal funnel were to break away from
 +
the peritoneum at a stage prior to that observed. There can be no
 +
question, however, but that the funnel-cords are outgrowths from the
 +
peritoneal funnels ; in fact their bases are the funnels themselves.
 +
 +
The distal portions of the funnel-cords lie above the vena cava in the
 +
fundament of the adrenal body, contributing the greater part of the tissue
 +
that in later stages constitutes the cortical substance of that gland. Peritoneal ingrowths may also be seen extending dorso-laterad from the
 +
peritoneum at a point near the base of the mesentery to the adrenal fundament. These also contribute to the cortical tissue of the adrenal body.
 +
They are of less regular occurrence than the funnel cords, and in later
 +
stages lose their connection with the peritoneum, although they are easily
 +
distinguishable in the stage of 7 mm. G-T. length.
 +
 +
The sex-gland can be clearly distinguished in the embryo of 6.8 mm.
 +
C-T. length. It extends through six somites, although the last ^ of it
 +
remains in a rudimentary condition. Even in this stage it consists
 +
merely of thickened peritoneum containing scattered primitive sex-cells
 +
(Ureier).
 +
 +
The sex-gland develops from a portion of the germinal epithelium lying
 +
between the bases of the funnel-cords and the base of the mesentery. In
 +
the embryo of 6 mm. C-T. length, a few primitive sex-cells were already
 +
beginning to appear in this region. At this time, the V. renalis revehens
 +
(vena cava) lies close above the germinal epithelium which has not yet
 +
begun to thicken to form the sex-cords. In an embrvo of 6.8 mm. C-T.
 +
length the germinal epithelium has sent out masses of cells towards the
 +
Y. renalis revehens, and has at the same time bent outward in such a
 +
manner as to form in transverse section, the periphery of a semi-circle,
 +
the interior of which is occupied by the sex-cords. The tips of the sex
 +
 +
 +
Bennet M. Allen 87
 +
 +
cords remain stationary and almost, if not quite, in contact with the
 +
wall of the V. renalis revehens, while their bases grow peripherally with
 +
the germinal epithelium. Mesenchyme cells between the sex-cords are
 +
few and far between.
 +
 +
At some points, the tips of the sex-cords penetrate to one side or the
 +
other of the Y. renalis revehens, and penetrate to the adrenal fundament
 +
to which they contribute.
 +
 +
Plate I, Fig. 3 shows a wax plate reconstruction of a large part of the
 +
sex-gland of the 7 mm. C-T. stage. In this stage the carapace has just
 +
formed. The prominent funnel-cords afford the most striking feature of
 +
the model. Their bases are attached to the peritoneum at the lateral
 +
boundary of the sex-gland. They extend in a dorso-medial direction.
 +
It will be noticed that each is connected with a primary Malpighian
 +
corpuscle. The other Malpighian corpuscles are not shown in the model.
 +
 +
Mediad of the funnel-cords the peritoneum is greatly thickened, forming numerous irregular elevations and ridges between which are deep
 +
clefts and pits. These thickenings are the sex-cords. They are solid
 +
and their cells show no evidence of a radial arrangement to form a
 +
lumen. The peritoneum is far more cut up than would appear from the
 +
model. Many slight fissures separating adjacent sex-cords do not appear.
 +
In any case many of these rudimentary sex-cords are from the first,
 +
united with the funnel-cords while others anastomose freely with one
 +
another, so that all are either directly or indirectly connected with the
 +
latter.
 +
 +
Primitive sex-cells are frequently met with in the germinal epithelium,
 +
as well as in the distal parts of the funnel-cords. Aside from the
 +
scattered primitive sex-cells, these tissues are composed of ordinary peritoneal cells. The cells of the germinal epithelium are so crowded as
 +
to make it stain very deeply. The sex-cords are less dense, their cells
 +
being distinct and having clear, sharp outlines, thus differing from
 +
those of the sex-cords of the pig and rabbit, in which a syncytium is
 +
formed among the pure peritoneal cells. The cells of all but the most
 +
proximal parts of the funnel-cord are elongated in the direction in which
 +
the cords extend. This elongation of the cells is so marked that they
 +
resemble the surrounding mesenchyme save for the fact that their cytoplasm is more dense thasi that of the latter. The cells are so closely
 +
associated that these funnel-cords stand out quite clearly from the surrounding mesenchyme.
 +
 +
The proximal part of each funnel-cord is met by one, or sometimes two,
 +
evaginations from the capsule of Bowman of the adjoining primary
 +
Malpighian corpuscle. These evaginations are very clearly distinguish
 +
 +
 +
88 The Eete-Cords and Sex-Cords of Chrysemys
 +
 +
able in this stage from the tissue of the funnel-cords but are in close
 +
contact with tliem.
 +
 +
In earlier stages the funnel-cords are not even in contact with the
 +
capsules of Bowman, although they lie close to them. In these stages
 +
there are no evaginations from the capsules of Bowman, although a
 +
thickening of the cells of the medio-dorsal portions of them indicates
 +
the general region where these evaginations will take place. In the much
 +
earlier stages described above, 6 mm. C-T., the primary union of Malpighian corpuscle, peritoneal funnel and funnel-cord has already been
 +
described. The later union of Malpighian corpuscle and funnel-cord
 +
is a secondary one, and has nothing to do with the temporary primary
 +
union. The breaking away and reuniting of these elements seems to be a
 +
useless process which I confess I am at a loss to explain. I can merely
 +
describe it. It is, however, a most easily demonstrated fact.
 +
 +
In later stages, the evaginations from the Malpighian corpuscles closely
 +
fuse with the funnel-cords, and are not to be distinguished from them.
 +
As development proceeds, the primary Malpighian corpuscles are often
 +
drawn some distance laterad of the sex-gland, at the same time pulling
 +
the funnel-cords laterad and causing them to stretch. In these cases
 +
each funnel-cord becomes sharply bent at the point where the evagination
 +
from the capsule of Bowman meets it; it is then continued in a dorsomedial direction to the adrenal body. As shown above, each primary
 +
Malpighian corpuscle is connected with the sex-cord by a cord of tissue,
 +
formed by an evagination from the capsule of Bowman plus the basal
 +
portion of a funnel-cord. These strands uniting the mesonephros with
 +
the sex-gland are the rete-cords and constitute the rete-testis or rete-ovarii,
 +
as the case may be. In these later stages the funnel-cords are more
 +
elongated and slender, but far more compact than in the early stages.
 +
 +
Plate I, Fig. 4 shows the rete-cords and the relation that they bear to
 +
the sex-cords and primary Malpighian corpuscles. Here the base of the
 +
funnel-cord lies within the sex-gland and forms one of the sex-cords.
 +
This has been observed in many cases. In very many instances, however,
 +
the funnel-cords lie wholly outside the sex-gland, their bases being still
 +
attached at a greater or less distance from the sex-gland to the peritoneum
 +
covering the mesonephros.
 +
 +
It will be noticed that the two rete-cords shown in this model are
 +
united to one another by a thickening of each in the direction of the
 +
long axis of the sex-gland. This represents a tendency to form a longitudinal canal uniting the rete-cords as in the Amphibia and to a certain
 +
extent in the Elasmobranchs, and in the lizard (Braun, 77). This
 +
 +
 +
 +
Bennet M. Allen " 89
 +
 +
longitudinal canal remains incomplete, however, although it may unite
 +
several rcte-eords in the manner shown.
 +
 +
Young males taken at the time of hatching, show many of the retccords to have already acquired a lumen in places. The rete-cords of
 +
females at this age do not show a lumen, nor do they at any time, because
 +
they have already paused in development. They are, however, still
 +
recognizable. Up to this point no distinction of sex has been noted
 +
although well marked differences had begun to appear in the stage of
 +
13 mm. C-T. length. Close study has yet to be made to determine the
 +
earliest evidences of sex differentiation.
 +
 +
It is not our aim to follow the later development of the rete-cords or
 +
sex-cords. In its general features, the further development of the sexglands of the turtle shows many points of similarity to that in the mammals. The sex-cords degenerate in the female forming the medullary
 +
cords while the " cords of Pfiiiger " arise as a later thickening of the
 +
germinal epithelium. In the males the sex-cords lengthen, assuming a
 +
more regular form and arrangement. Their thorough anastomosis with
 +
one another allows the semen to be poured from several into a common
 +
rete-cord. The mesonephros degenerates leaving a number of the uriniferous tubules to function as vasa efferentia. In the adult male the retecords are found to be reduced in number, there being nine in the specimen
 +
studied while sixteen were counted on the right side of an embryo of
 +
C-T. 8 mm. length. No attempt was made to determine how or when
 +
this reduction was brought about. It is quite probable that some retecords are weak and become broken by shifting of the organs in the
 +
process of growth. In any case there is no systematic degeneration of
 +
the rete-cords in any particular region or regions along the sex-gland.
 +
 +
Summary and Conclusions.
 +
 +
The sex-cords are formed from irregular ingrowths of the germinal
 +
epithelium. It is not until relatively late in development that they take
 +
on the semblance of cords. They are made up of ordinary peritoneal cells,
 +
together with primitive sex-cells which are also found in the peritoneum
 +
at this stage.
 +
 +
The rete-testis and rete-ovarii are formed by the union of funnel-cords
 +
with evaginations from the capsules of Bowman. The funnel-cords are
 +
derived from the peritoneal funnels of the Malpighian corpuscles. They
 +
occupy a region lying along the lateral edge of the sex-gland, and not only
 +
co-extensive with the latter, but extending a short distance anterior to
 +
it. The bases of the funnel-cords may, or may not, be included in the
 +
sex-gland to form a part of the seminiferous tubules of the testis or
 +
 +
 +
 +
90 The Eete-Cords and Sex-Cords of Chrysemys
 +
 +
iiRHluUary cords; of the ovary, as the case may he. The ])roxiinal portions
 +
of these fi)nnel-cords go to form a large part of the rete-testis-ovarii,
 +
while the more distal portions join the adi'onal fundament and contril)nte
 +
the major portion of the cortical suhstance of that organ.
 +
 +
This leads me to briefly consider the adrenal body, although this was
 +
not within the original plan of the present work. Soulie, 02, finds that
 +
in Lacerta and the chick, the cortical substance arises wholly from cords
 +
of cells proliferated from the peritoneum mediad of the sex-gland and
 +
at the base of the mesentery. He states, however, that these cords become
 +
closely applied to the capsule of Bowman of the Malpighian corpuscle.
 +
It is difficult to understand how, arising from the base of the mesentery,
 +
they could reach the Malpighian corpuscle without growing dorsad along
 +
the medial side of the Y. renalis revehens to the adrenal body fundament,
 +
and thence laterad and ventrad to the Malpighian corpuscle. It is difficult to understand how they could take this course, without passing
 +
through and beyond the fundament of the adrenal body. There certainly
 +
are, in the turtle, cords of cells that arise as Soulie and others claim, near
 +
the base of the mesentery, and these contribute to the formation of the
 +
adrenal body; but certain sex-cords and the funnel cords contribute to
 +
it as well, and in even greater measure. Brauer, 02, also holds a view
 +
similar to that of Soulie as regards Hypogeophis one of the Gymnophiona.
 +
Poll, 03, reached similar results with the Elasmobranchs, Acanthias and
 +
Spinax. Be this as it may, I feel quite sure of my ground in the case of
 +
Chrysemys, and the work of Weldon, 85, and Hoffmann, 89, would lend
 +
color to this view, though they hold views in some points radically different from mine.
 +
. In this connection it may be well to state that several of Hoffmann's,
 +
89, figures of the " Sexual Strange " would serve fairly well to represent
 +
the funnel-cords as I have seen them. They certainly do not prove
 +
his contention that the cords in question, sex-cords and adrenal-cords,
 +
arise from the capsule of Bowman ; although he has so interpreted them.
 +
 +
Those who held view C, probably used insufficient material and lacked
 +
the intermediate stages between the period just before the formation of
 +
the sex-cords and those subsequent to their separation from the germinal
 +
epithelium.
 +
 +
Janosik, 85, D, worked upon the chick. It is quite possible that future
 +
work may in large part substantiate his results for that form. My results
 +
agree with his as regards the origin of the sex-cords,- but differ from his
 +
upon the origin of the rete-tissue, although even here there may be a
 +
reconciliation between our views.
 +
 +
In the literature upon the morphological significance of the uro-genital
 +
 +
 +
 +
Bennct M. Allen
 +
 +
 +
 +
91
 +
 +
 +
 +
system we have some melancholy examples of the futility of making
 +
rash hypotheses unsupported by a sufficient array of facts. Still it is
 +
of interest to consider the possible interpretation that may be placed
 +
upon these structures when they are viewed from the standpoint of
 +
phylogeny.
 +
 +
I am inclined to consider tlie funnel-cords as modified sex-cords. The
 +
fact that their distal extremities contribute to the formation of tbe
 +
adrenal bodies does not conflict with this interpretation, because that is
 +
also true of undoubted sex-cords. The funnel-cords arise just laterad of
 +
the true sex-cords and in a very similar manner. The fact that they
 +
arise from the peritoneal funnel would not be contrary to this view if the
 +
 +
 +
 +
 +
Text Fig. F. Diagram to show essential structures of the mammalian
 +
 +
 +
 +
sex-gland.
 +
 +
M. — Mesonephros.
 +
MP. — Malpighian corpuscles.
 +
 +
R. — Rete-region.
 +
R. C. — Rete-cord.
 +
 +
S. — Sex-gland region.
 +
 +
 +
 +
8. C— Sex-cord.
 +
 +
y. — Vestigial portion of genital
 +
ridge.
 +
W.D.— Wolffian duct.
 +
 +
 +
 +
funnels could be shown to be mere recesses of the peritoneum, and similar
 +
to the latter in histological character. A more careful study of the
 +
origin of the sex-glands in the Amphibia is much to be desired as it might
 +
throw new light upon this question. It will be of interest to compare
 +
the results of this paper with those of a previous paper upon the same
 +
structures in the pig and rabbit. Allen, 04.
 +
 +
The very schematic diagram of the testis of the pig (Text Figure F),
 +
shows the following points seen in a sagittal section passing through the
 +
genital ridge and the mesonephros. The genital ridge may be divided
 +
into three regions : (1) rete, (3) sex-gland, (3) rudimentary sex-gland
 +
ridge. The rete-cords arc liomodynamous with the sex-cords, being formed
 +
at the same time and in tlie same luannei" as the latter, and occupying
 +
 +
 +
 +
92 The Eete-Cords and Sex-Cords of Chrysemys
 +
 +
the anterior third of the genital ridge, whose middle portion is occupied
 +
by the sex-gland. As the rete-cords develop, they come in contact with
 +
slight evaginations from the Malpighian corpuscles in that part of the
 +
mesonephros which lies nearest the rete region. They then grow back
 +
to the anterior portion of the sex-gland and at a relatively late ]ierio(l
 +
of development advance along its entire length, giving off numerous
 +
branches (tubuli recti) which fuse with the tips of the seminiferous
 +
tubules. The rete-cords of the mammals are the peritoneal ingrowths of
 +
the anterior part of the genital ridge. Speaking in terms of phylogeny
 +
they are the sex-cords of the anterior part of the sex-gland. The analogous structures of the turtle, the funnel-cords, appear at intervals along
 +
the entire lateral margin of the sex-gland.
 +
 +
It is quite probable that the mammalian sex-gland was derived from
 +
that of some reptilian group and that some now existing groups of reptiles
 +
may show sex-gland conditions from which those of the mammals were
 +
derived. ]S[o existing group is more likely to show mammalian affinities
 +
than that of the Chelonia.
 +
 +
jSTothing exactly corresponding to the funnel-cord has ever been found
 +
in the embryonic development of the mammals. It is true that Aichel,
 +
00, has found that the cortical portion of the adrenal body of the rabbit
 +
(Lepus) arises from funnel-like invaginations of the peritoneum near
 +
the base of the mesentery. He is very positive in his claim that these
 +
are the peritoneal funnels of the mesonephros. Nevertheless, he does not
 +
claim to have followed these funnels back to stages in which they were
 +
actually connected with the Malpighian corpuscles. The rete-tubules that
 +
may have directly united the sex-gland proper along its entire length with
 +
the adjacent Malpighian corpuscles of the mesonephros have disappeared
 +
without leaving a recognized vestige, in any of the mammals thus far
 +
studied. The rete-region of these mammals has been evolved from that
 +
part of the genital ridge which was primitively the anterior part of the
 +
sex-gland in the ancestors of the mammals.^
 +
 +
It is scarcely possible to be more specific as regards the nature of the
 +
rete-region of the mammals. Two assumptions are possible: one, that
 +
 +
^ It will be well to note that in Chrysemys, several funnel-cords occur in
 +
a well-marked region, anterior to the sex-gland, in which the sex-cords remain
 +
vestigial. Upon closer study of some sagittal sections of the sex-gland and
 +
mesonephros of Chrysemys I have been struck with the resemblance that
 +
this region bears to the rete-region of the pig and rabbit as seen in similar
 +
sections. In Chrysemys the funnel-cords of this anterior region together with
 +
those of the sex-gland region are joined to form the central canal. This
 +
shows some points of resemblance to the portions of the rete-cords lying
 +
parallel to the peritoneum anterior to mammalian sex-gland.
 +
 +
 +
 +
Bennet M. Allen ' 93
 +
 +
the sex-cords have disappeared leaving only the funnel-cords, the other,
 +
that the sex-cords which primitively existed in this region have taken
 +
on the character and function of funnel-cords. It is difficult to decide
 +
this question, I can merely say that the latter assumption seems the
 +
more probable one, because often two or more rete-cords can be seen in
 +
a single transverse section to arise from more than one point of the
 +
peritoneum covering the rete ridge. In fact the strongest and most numerous rete-cords arise from the portion of it that lies nearest the mes'entery. This question might be solved with certainty by a study of the
 +
conditions in the Monotremes or even in other less primitive groups of
 +
mammals.
 +
 +
LITERATURE CITED.
 +
 +
AiCHEL, Otto, oo. — Vergleichende Entwickelungsgeschichte unci Stammes
 +
geschichte der Nebennieren. Arch. f. mikr. Anat., Bd. LVI, 1900.
 +
Allex, B. M., 04. — The Embryonic Development of the Ovary and Testis of
 +
 +
the Mammals. American Journal of Anat., Vol. Ill, 1904.
 +
Balfour, P. M., 78. — A Monograph of the development of the Elasmobranch
 +
 +
Fishes. Works of F. M. Balfour, 1878.
 +
Braux, M., 77. — Das Urogenitalsystem der einheimischen Reptilien. Arb.
 +
 +
Zool.-zoot. Institut, Wiirzburg, Bd. IV, 1877.
 +
HoFFMAxx, C. K., 89. — Zur Entwickelungsgeschichte der Urogenitalorgane bei
 +
 +
den Reptilien. Zeitschr. f. wiss. Zool., Bd. XLVIII, 1889.
 +
 +
92. — Sur le developpement de I'appareil. uro-genital des oiseaux. Verb.
 +
 +
d. Koninklyke Akademie v. Wetenschappen te Amsterdam. Sectie 2,
 +
Deel I, No. 4, 1892.
 +
Jaxosik, J., 85. — Histologisch embryologische Untersuchungen iiber das Urogenitalsystem. Sitz. Ber. Akad. Wien, 3 Abth., Bd. XCI, 1885.
 +
 +
90. — Bemerkungen iiber die Entwickelung des Genital Systems. Sitz.
 +
 +
Ber. Akad. Wien, 3. Abth., Bd. XCIX, 1890.
 +
Laulaxie. F., 86. — Sur le mode d'evolution et la valeur de I'epithelium germi
 +
natif dans le testicule embryonnaire du Poulet. C. R. Soc. de
 +
 +
Biologie, T. 3, 1886.
 +
Mihalkovics, v., 85. — Untersuchungen iiber die Entwickelung des Harn- und
 +
 +
Geschlechtsapparates der Amnioten. Inter. Monatschr. f. Anat. Hist.,
 +
 +
Bd. II, 1885.
 +
MoLLER, F. v., 99. — Ueber das Urogenitalsystem einiger Schildkrbten. Zeitschr.
 +
 +
f. wiss. Zool., Bd. LXV, 1899.
 +
 +
The most plausible theory is that the rete-region of the mammals has not
 +
been directly derived from a condition like that in Chrysemys; but that the
 +
genital ridges of both have been derived from a type in which the anlage of
 +
the sex-cords was co-extensive with that of the funnel-cords.
 +
 +
To be more exact then, the rete-region of the mammals corresponds to the
 +
anterior end of the sex-gland of the turtle plus the modified region of funnelcords anterior to it.
 +
 +
 +
 +
94 The Rete-Cords iind Sex-Cords of Chrysemys
 +
 +
Peter, K., 04. — Normeiitafel ziir Eutwickelungsgeschichte der Zauiieidechse
 +
 +
(Lacerta muralis). Normentafeln z. Entw. gesch. d. Wirbelthiere,
 +
 +
Heft IV, 1904.
 +
Poll, H., 03. — Die Anlage der Zwischenniere bei den Haifischen. Arch. f.
 +
 +
mikr. Anat., Bd. LXII, 1903.
 +
SCHMIEGELOW, E., 82. — Studien iiber die Entwickelung des Hodens und Neben
 +
hodens. His u. Brawne Archiv f. Anat. u. Physiol., 1882.
 +
Semox, R., 87. — Die indifferente Anlage der Keimdriisen beim Hiihnchen und
 +
 +
ihre Differenzierung zum Hoden. Jena Zeitschr. f. Naturwiss., Bd.
 +
 +
XXI, 1887.
 +
 +
90. — Ueber die morphologische Bedeutung der Urniere in ihrem Ver
 +
haltniss zur Vorniere und Nebenniere und iiber ihre Verbindung mit
 +
dem Genitalsystem. Anat. Anz., Bd. V, 1890.
 +
 +
91. — Studien iiber dem Bauplan des Urogenitalsystems der Wirbel
 +
thiere. Jenaische Zeitschr. f. Naturwiss., Bd. XXVI.
 +
Semper, C, 75. — Das Urogenitalsystem der Plagiostomen. Arb. Zool.-zoot.
 +
 +
Institut, Wiirzburg, Bd. II, 1875.
 +
SovLiE, 04. — Recherches sur le developpement des capsules Surrenales chez
 +
 +
les vertebres Superieurs. Journ. de I'Anat. et de la Phys., T. 39.
 +
Waldeyer, W., 70. — Eierstock und Ei. Leipzig, 1870.
 +
Weldon, W. F. R., 85. — On the Suprarenal Bodies of the Vertebrata. Quart.
 +
 +
Journ. of Micr. Sci., Vol. XXV, 1885.
 +
 +
EXPLANATION OF PLATE.
 +
 +
Ao. — Aorta. P. — Peritoneum.
 +
 +
Art. — Arterial branch passing to P. F. — Peritoneal funnel.
 +
 +
the Malpighian corpuscle. P. C. — Posterior cardinal vein
 +
 +
FC. — Funnel-cord. 8C. — Sex-cord.
 +
 +
M. — Mesentery. Y. R. R. — V. renalis revehens.
 +
 +
M.C. — Malpighian corpuscle. W.D. — Wolffian duct.
 +
 +
Fig. 1. Transverse section of the mesonephros and sex-gland fundament
 +
of an embryo of 6 mm. C-T. length. X 190.
 +
 +
Fig. 2. Transverse section of the sex-gland fundament of an embryo of
 +
7 mm. C-T. length (carapace 5 mm. long). X 190.
 +
 +
Fig. 3. Wax plate reconstruction of the indifferent sex-gland of an embryo of 7 mm. C-T. length (carapace 5 mm. long). This includes as much of
 +
the sex-gland as lies within a little more than two somites. X 190.
 +
 +
Fig. 4. Reconstruction of a small part of the sex-gland of an embryo of
 +
13 mm. C-T. length (carapace 12 mm. long). X 190.
 +
 +
Fig. 5. Drawing of a part of a section adjacent to that shown in Fig. 1.
 +
The proximal portion of the peritoneal funnel is here better shown than in
 +
Fig. 1.
 +
 +
 +
 +
THE RETE-CORDS AND SEX-CORDS OF CHRYSEMYS
 +
 +
BENNET M. ALLEN
 +
 +
 +
 +
%tt 4 "% '
 +
 +
 +
 +
^ »> J
 +
 +
 +
 +
 +
 +
 +
 +
AMERICAN JOURNAL OF ANATOMY~VOL. V
 +
 +
 +
 +
PLATE I
 +
 +
 +
 +
 +
 +
F,g4
 +
 +
 +
 +
THE DEVELOPMENT OF THE LYMPHATIC SYSTEM IN
 +
 +
BABBITS.
 +
 +
BY
 +
 +
FREDERIC T. LEWIS, A.M., M. D.
 +
 +
From the EmbryoJogical Laboratory, Harvard Medical School.
 +
 +
With 8 Text Figures.^
 +
 +
In following the transformations of the subcardinal veins in rabbits,
 +
the writer observed that a portion of those veins seemed to become detached from the venous system, and to be transformed into l3'mphatic
 +
vessels (02, p. 238). This supposition is not identical with the theory
 +
that the lymphatic system is a gland-like outgrowth of venous endothelium, always connected with the veins by means of the lymphatic ducts.
 +
It differs also from the older idea that lymphatic vessels are excavations
 +
in mesenchyma.
 +
 +
In favor of this mesenchymal origin, the work of Sala, 00, is the most
 +
convincing. He observed in the chick that both the posterior lymph heart
 +
and the thoracic duct arose independently of the veins or of other lymphatics, and that their permanent openings into the veins were acquired
 +
subsequently. In the rabbit, as will be shown presently, there are many
 +
disconnected lymphatic spaces, but to their origin from mesenchyma there
 +
are four objections : 1st. The lymjjhatic spaces do not resemble mesenchyma even when it is cedematous, but on the contrary, are scarcely distinguishable from blood-vessels (Langer). 2d. After being formed, the
 +
lymphatics increase like blood-vessels, by means of blind endothelial
 +
sprouts, and not by connecting with intercellular spaces (Langer, Eanvier,
 +
MacCallum, Sabin). 3d. In early embryos, detached blood-vessels may
 +
be seen without proving that blood-vessels are mesenchymal spaces. These
 +
detached vessels are not far from the main trunks, from which they may
 +
have arisen by slender endothelial strands, yet often the connecting
 +
strands cannot be demonstrated. A similar supposition would account
 +
for detached lymphatic vessels. 4th. The endothelium of the embryonic
 +
lymphatics is sometimes seen to be continuous with that of the veins.
 +
 +
^ This investigation, and the one which follows, were accomplished with
 +
the aid of a Bullard Fellowship, established in memory of John Ware.
 +
Ameeican Journal of Anatomy. — Vol. V.
 +
 +
 +
 +
96 The Development of the Lymphatic System in Eabbits
 +
 +
The second thcoiy, that of the giand-like origin of the lymphatic system,
 +
is supported by the remarkable injections of pig embryos, made by
 +
Prof. Sabin.' She considers that in mammals, this system buds from the
 +
venous endothelium at four points, forming four lymphatic ducts. The
 +
ducts are dilated to form four lymph hearts, which, though destitute of
 +
muscles, correspond with the four lymph hearts of amphibia. Starting
 +
from these hearts, lymphatic outgrowths invade the body, and those from
 +
the anterior pair unite with those from the posterior pair. Then the
 +
posterior hearts lose their original openings into the veins, but those of
 +
the anterior hearts persist as the outlets for the thoracic and right lymphatic ducts respectively. The lymph hearts themselves are said to become transformed into lymph nodes (05, p. 355).
 +
 +
According to this idea, the lymphatic vessels are true lymphatics from
 +
their earliest inception. They differ from other branches of the veins
 +
by their very oblique angle of entrance, and by failing to anastomose with
 +
arteries or veins. Anastomoses with other lymphatics are abundant, due
 +
to absorption of contiguous walls (Ranvier, 97, p. 74).
 +
 +
The supposition suggested by the study of the subcardinal veins is
 +
intermediate between those of Sabin and Sala. The endothelium of
 +
the lymphatics is considered to be a derivative of that which lines the
 +
veins, since the lymphatics are at first a part of the venous system; but
 +
by becoming detached from their origins these lymphatics form closed
 +
sacs in the mesenchyma. Later they acquire permanent openings into
 +
the veins, and many connections with other lymphatics.
 +
 +
In studying the development of the lymphatic vessels, several methods
 +
have been employed. Sala used serial sections, generally of injected
 +
embryos, and made wax reconstructions of the posterior hearts. Sabin
 +
perfected the method of injection whicli had been employed by Ranvier
 +
for pigs of 100 mm., so that it was applicable to those of 20 mm. By
 +
this means she studied the large jugular hmpli sacs, or " anterior hearts,"
 +
which, as Saxer discovered (p. 370), are the earliest lymphatic vessels
 +
to appear. On the basis of injections she was enabled to present the
 +
first connected account of the development of the mammalian Ij^mphatic
 +
system. This was illustrated by a series of conventional diagrams, in
 +
which the blood-vessels are shown without details. Thus the internal
 +
 +
^ Ranvier described the interesting analogies, botli functional and embryological, between typical glands and the lymphatic system. Sabin does not
 +
adopt the idea that the whole lymphatic system represents a few large
 +
glands. She does, however, describe it as arising from four blind epithelial
 +
(endothelial) outpocketings which ramify in the connective tissue, and this
 +
origin may be designated, after Ranvier, as " gland-like."
 +
 +
 +
 +
Frcdoi'ic T. Lewis
 +
 +
 +
 +
97
 +
 +
 +
 +
and t'xtonial juuular veins are merged in an '" anterior cardinal vein,"
 +
the subcardinals are omitted, the renal and iliac anastomoses are made
 +
continuous with one another, and the sciatic and femoral veins are
 +
reversed.
 +
 +
 +
 +
 +
Fig. 1. Rabbit, 13 days, 9.5 mm.. Harvard Embryological Collection, Series
 +
498, X 13 diams. 3, 4, and 5 indicate the position of the corresponding
 +
cervical nerves in this, as in the following figures. The veins shown are
 +
those of the left side: D. C. duct of Cuvier; Ex. M.. external mammary;
 +
In. J., internal jugular; Pr. VI., primitive ulnar.
 +
 +
 +
 +
It was thought that more accurate figures might bo obtained bv tlie
 +
graphic reconstruction of uninjected embryos. The possibility of overlooking minute orifices guarded by valves, and the limitation of this
 +
method to small embryos are obvious disadvantages, but these are offset
 +
7
 +
 +
 +
 +
98
 +
 +
 +
 +
Tlie Dovclojiiiu'iit of tlio Lyniitliatic Svf^tein in Kabbits
 +
 +
 +
 +
l)v the avoidance of rnptnre of very thin-walled vessels and by the opportunity of seeing lymphatics too small for injection. The method has
 +
been employed ^Yith the following results.
 +
 +
 +
 +
 +
Fig. 2. Rabbit, 14 days, 10 mm., H. E. C, Series 155, X 13 diams. The
 +
lymphatic vessels are lieavily shaded, as in all the following figures. The
 +
veins are those of the left side: An. T., anterior tibial; C, caudal: c. b..
 +
" connecting branch "; D. C. duct of Cuvier; Ex. J., external jugular; Ex. M.,
 +
external mammary; In. J., internal jugular; P.O., posterior cardinal; Pr.Fi.,
 +
primitive fibular; Pr. VI., primitive ulnar.
 +
 +
In a rabbit of 13 days, 9.5 mm., no lymphatics could be found. The
 +
reconstruction, Fig. 1, shows the veins along which the first lymphatics are
 +
soon to appear. The internal jugular vein receives a great many small
 +
 +
 +
 +
Frederic T. Lewis 99
 +
 +
branches. One of these, nearly parallel with the dorsal border of the vein
 +
and wider than the others, opens into the vein at either end. It is in
 +
relation with the third cervical nerve. From its position and appearance
 +
it is believed that this branch of the vein becomes a lymphatic vessel.
 +
 +
The second reconstruction is a 10 mm. embryo of 14 days. In this
 +
specimen a chain of lymphatic spaces has appeared along the internal
 +
jugular and the dorsal root of the primitive ulnar veins. The most
 +
anterior segment of the chain extends back to the third cervical nerve. It
 +
sends out short blind sprouts like a vein and contains many blood
 +
corpuscles. The partition between it and the jugular vein is very thin,
 +
and at one point there is a suggestion of communication between the
 +
two, as shown in the figure. No opening into the vein can be demonstrated, however. The second segment of the chain, proceeding posteriorly, extends to the fifth nerve. It equals the internal jugular vein in
 +
diameter, and is closely applied to its wall. Behind the third nerve it
 +
sends a blind diverticulum around the ventral end of the dorsal body
 +
muscles, into the deep subcutaneous tissue of the back. This diverticulum, not matched on the opposite side of the embryo, contains blood
 +
which apparently entered it from rough treatment in preserving the
 +
specimen. The third segment of the chain, between the fifth and sixth
 +
nerves, seems to connect with the root of the ulnar vein. This connection,
 +
however, lies in the plane of section, and a thin intervening wall may have
 +
been carried away in the process of cutting. A detached lymph space
 +
follows the dorsal root of the ulnar vein. A small and somewhat questionable one, not matched on the opposite side, rests against the superior
 +
vena cava, between the roots of the ulnar vein. The most significant
 +
structure found in this embryo is a space filled with blood, which opens
 +
into the external jugular vein near its junction with the internal jugular.
 +
This space lies quite near the third segment of the lymphatic chain. On
 +
the opposite side of this embryo, and in the following one, this blood-filled
 +
sac connecting with the vein appears to . be replaced by a lymphatic
 +
space, detached from the vein, but connecting with the chain.
 +
 +
Fig. 3. from an embryo of 1-1 days, 11 mm., shows the fusion of all the
 +
lymphatics of the previous stage into one large sac which encircles the
 +
external jugular vein. On neither side could this sac be seen to communicate with the veins. No lymphatic vessels were found which did not
 +
connect with the jugular sacs. The dorsal subcutaneous extension, described in the preceding stage, occurred on both sides. In the posterior
 +
part of the embryo, no lymphatics were found. The reconstruction of
 +
the cardinal veins is that already figured in this journal. Vol. I, Plate
 +
2, Fig. 5 (following p. 244).
 +
 +
 +
 +
100 The Development of the Lymphatic System in Rabbits
 +
 +
 +
 +
The cardinal veins of the 14.5 mm. rabbit, Fig. 4, were also shown in
 +
the earlier paper (Plate 3, Fig. 7). In the plate, the lower portions of
 +
the subcardinal veins are detached from the rest, and, though colored blue
 +
 +
 +
 +
 +
Fig. 3. Rabbit, 14 days, 11 mm., X 13 diams. The structures drawn are
 +
the same as in Fig. 2, except that in the trunlt of the embryo the following^
 +
veins, belonging to the median plane and to the right side, have been added:
 +
Az., azygos; G., gastric; R. A., renal anastomosis of the subcardinal veins;
 +
Sc, subcardinal; /S. M., superior mesenteric; V., vitelline; V. G. I., vena cava
 +
inferior.
 +
 +
 +
 +
Frederic T. Lewis 101
 +
 +
■ like the veins, they are deseribed and figured as " spaces in the mesentery "
 +
suggesting the lymph hearts of the chick (p. 238). It is stated that
 +
these spaces " may be siibcardinal derivatives." Ee-examination of this
 +
embrj'O has yielded no more definite information. The spaces which are
 +
midoubtedly lymphatic, as shown by their later development, seem to replace veins of the preceding stage. In the same way the lymphatic
 +
vessels in the mesentery, accompanying the superior mesenteric ancVthe
 +
gastric veins may have arisen as the branches of those vessels seen in
 +
Fig. 3. They extend around the superior mesenteric artery, which the
 +
corresponding vein accompanies. The fused vitelline vein is destitute of
 +
small branches, and is not provided with lymphatics.
 +
 +
The jugular lymph sac in Fig. -t has completely surrounded the third
 +
and fourth cervical nerves. It envelops two-thirds of the circumference
 +
of the internal jugular vein. On the right side of the embryo, in one
 +
section (No. -176), a miniite orifice connected the sac and the vein.
 +
It was not in the position of the adult opening between these structures,
 +
and was not matched on the opposite side. The deep subcutaneous outgrowth from the jugular sac has become greatly dilated in its distal portion.
 +
Near the beginning of the external mammary vein, a large lymph space
 +
is found wedged between two converging venous branches. This space is
 +
not connected with the veins. It may be a remnant of the lymphatic
 +
vessels which in the preceding stage accompanied the dorsal root of the
 +
ulnar vein. A few slender detached lymphatics follow the external
 +
mammary vein. Finally there are two lymphatics which appear to have
 +
arisen from branches of the azygos vein, one near the vagus nerve (Fig.
 +
4, x) and the other along the aorta (Fig. 4, y). The former connects
 +
with a small vein, the latter ends blindly not far from one. Obviously
 +
when a connection with a vein is well preserved the structure in question
 +
would be considered a venous branch; and after becoming detached, were
 +
it not for its endothelial wall, it might be called a mesenchymal excavation. The study of this and the following specimens seems to show that
 +
the lymphatics along the aorta (thoracic ducts) are derived in part from
 +
the azygos veins; below, from the subcardinals; and above, from the
 +
jugular sacs.
 +
 +
In order to determine whether the lymphatic system of the rablut
 +
differed materially from that of other mammals, reconstructions were
 +
made of a 21 mm. pig, and a 15 mm. cat. The former is of special
 +
interest as a basis of comparison between the present work and > that of
 +
Prof. Sabin. The lymphatics in the pig (Fig. 5) consist of a pair of
 +
jugular lymph sacs, a pair of subcardinal sacs which fuse with one
 +
another irregularly and are variously subdivided by thin septa, and
 +
 +
 +
 +
 +
Fic. 4. Rabbit. 14 days 18 hours, 14.5 mm., H. E^ C Sen^^l^^'^^,/,":
 +
diams. X designates a lymphatic vessel '-^^^^^P^!^^"^?^ f ^/^ Jf ceiSc;
 +
.,. a lymphati^c along ^^e aorta. The veans of th -^^,^%^,_ ,;
 +
Pr. Ul.. primitive ulnar. Those ot tne leg axe. ^-i". ^ ••
 +
"connecting branch"; Fe., femoral; Pr. Fu, primitive fibular.
 +
 +
 +
 +
Frederic T. Lewis ' 103
 +
 +
finally some irregular spaces behind the aorta, probably derived from the
 +
azygos veins. These spaces also fnse across the median line at several
 +
points.
 +
 +
The jngnlar sac is shaped like a D of which the chief portion is vertical
 +
and closely applied to the internal jugular vein. Through the aperture
 +
in the D pass the third, fourth, and fifth cervical nerves, and from its
 +
dorsal arch several deeply subcutaneous sprouts pass off, corresponding
 +
with the single large sac of the rabbit. ISTo connection between the
 +
jugular sac and the veins could be detected. Except for this point, the
 +
reconstruction agrees with, and combines, the figure and diagram presented by Prof. Sabin in this journal. Vol. 3, p. 184, and Vol. -1, p. 359.
 +
It does not agree so well with the diagram on p. 380 of A^ol. I. In the
 +
latter the subcardinal l3anph spaces are not shown. The posterior portion
 +
of the body contains instead two " lymph hearts " arising from tlie posterior cardinal veins " below the Wolffian body "" but anterior to the
 +
femoral vein. In later stages, outgrowths from these hearts invade the
 +
skin of the back, and nltimately, as has already been noted. Prof. Sabin
 +
considers that the hearts become transformed into lymph nodes. From
 +
this description, it appears that the posterior lymph hearts are in the
 +
position of the ilio-lumbar veins. In the pig embryo represented in Fig.
 +
5, however, no lymphatics were found in relation with the ilio-lumbar
 +
vessels.
 +
 +
Considering its lymphatic development the pig of 21 mm. is less advanced than the rabbit of 14.5 mm., since there are no lymphatic vessels
 +
along the external mammary vein nor in the mesentery. The cat of
 +
15 mm. is more advanced than either. In this embryo the D formed by
 +
the jugular sac is almost bisected diagonally. The second, third, and
 +
fourth nerves pass through its aperture, but the fifth penetrates the
 +
posterior section of the sac by a separate opening. There are two deep
 +
subcutaneous diverticula corresponding with the single one in the rabbit
 +
and several in the pig. In one section (266) a branch of the jugular
 +
sac may enter the innominate vein a little anterior to the subclavian, but
 +
it is not clear that an actual opening exists and none can be found on
 +
the opposite side.
 +
 +
"Where the external mammary vein joins the brachial there is a large
 +
sac, and the question arises M'hether or not the detached lymphatics following the mammai-y vein are independent formations, or are outgrowths
 +
from that sac. The occurrence of the lymphatics especially near the
 +
places where the veins l)ranch suggests that they may have budded at such
 +
points. On the other hand, as in the rabbit, their order of appearance
 +
is from the proximal ])art of the vein distally. Similarly there are
 +
 +
 +
 +
 +
vf w r Series 59, X 10 diams. The veins are:
 +
 +
Fig. 5. Pig, 20 mm.. H. E. C., series oy, ^ Cuvier; Ea-. J., exter
 +
A^., azygos; Br., brachial: Ce cephalic D. ^^^^^^ f ^^'^^ _ ^^stric; In. J.,
 +
 +
nal' jugular: i... M -^--^^ ^.^^^rn'osifof ^b'ardinals- ..n.. sciatic;
 +
 +
;rS!tp^e^S?;ies?nte^riV;?^^viSlfne; V. C. I. vena cava inferior.
 +
 +
 +
 +
 +
Fig. 6. Cat. 15 mm., H. E. C, Series 436, X 13 diams. The lettering Is
 +
the same as in Fig. 5.
 +
 +
 +
 +
10() The Development of the Lymphatic System in Eabbits
 +
 +
obscure spaces which ap})ear to ho lymphatic, along the aorta, and in relation with the azygos veins. An occasional apparent connection with
 +
the vein suggests their venous origin in situ. The mesenteric and subcardinal plexuses have united with one another. They do not empty into
 +
the veins. The subcardinal sacs extend from the renal anastomosis
 +
almost to the sciatic vein, connecting with one another across the median
 +
line, as in the pig. No lymphatic vessels follow tlie ilio-lumbar veins
 +
into the posterior body wall.
 +
 +
Eeturning to the rabbit embryos it will be seen that Fig. 7 from a
 +
21 mm. rabbit differs from Fig. 4, the 14.5 mm. embryo, chiefly in regard
 +
to the thoracic duct. The duct is represented by a pair of vessels which
 +
connect with one another and pass on to the left jugular sac. Sometimes
 +
in the adult rabbit, as figured by Gage (02, p. 650), and occasionally in
 +
man, the thoracic duct bifurcates anteriorly and passes to the jugular
 +
sacs on either side. This did not occur in the 21 mm. embryo, which
 +
exhibited the relations figured by Sabin, Vol. I, p. 383.
 +
 +
In Fig. 7 scattered lymphatics are shown along the external jugular
 +
vein and its branches. One much larger than the rest occurs wdiere the
 +
anterior and posterior facial veins unite. From its isolation it probably
 +
arose independently of the large jugular sac. Other and more isolated
 +
lymphatic centers are seen in the oldest rabbit studied, one of 20 days,
 +
29 mm., Fig. 8, notably along the pudic and the sciatic veins. They arise
 +
near the junction of several venous branches, with which, however, they
 +
are not in communication.
 +
 +
In the oldest embryo the lymphatic system has invaded the skin to such
 +
an extent that it is impracticable to represent more than a small part of
 +
it. In entering the skin the lymph vessels accompany the veins, those
 +
of the head following chiefly the external jugular vein. The jugular sac
 +
has become relatively less important, and persists as the lymphatic sheath
 +
of the internal jugular vein. The deep subcutaneous extension has
 +
becom^ covered by a thin layer of muscle, presumably the panni cuius,
 +
and does not appear to connect with the more superficial vessels of the
 +
skin. There are no lymphatics in the distal part of the arm, Init the subcutaneous vessels of the shoulder are attended by rich netw^orks. These
 +
veins are the external mammary, and another w^hich is ventral to the
 +
scapula and posterior to the shoulder joint, — a subscapular vein. The
 +
lymphatics along this large subscapular vein do not connect with the
 +
jugular sac. At the point L. N., indicated in the figure, a small but
 +
very distinct lymph node has developed in relation to these subscapular
 +
lymphatics. A corresponding node is found on the opposite side of the
 +
body.
 +
 +
 +
 +
 +
Fig. 7. Rabbit, 17 clays, 21 mm., H. E. C, Series 738, X 10 diams. The
 +
veirs not previously lettered in the rabbit figures are: 7?., ilio-lumbar; Ss.,
 +
subscapular; R., radial; Sci., sciatic.
 +
 +
 +
 +
 +
i^Z u ^^^'Y- ^^ ^f^^' ^^ "'"'•• ^- ^- C- Series 170, X 6.9 diams. The
 +
first lymph nodes develop at L. lY., along the subscapular vein. ^^s. ; and at
 +
J. «., along the iho-lumbar vein, II. The veins of the arm are: Br., brachialte cephalic: J. Ce.. jugulo-cephalic: R.. radial. Those of the legs are- A« T
 +
anterior tibial; Sci., sciatic: Po. T.. posterior tibial: Fe.. femoVal; c. &., connecting branch between femoral and sciatic. P. marks the pudic vein
 +
 +
 +
 +
Frederic T. Lewis 109
 +
 +
The jugular sac on the leCt side, except for an extensive rupture, does
 +
not connect with the vein. On the right, a pore is found leading from
 +
the sac to the internal jugular vein near its union with the external, but
 +
this also may he artificial. Thus in all the series of rabbits no bilateral
 +
communication of the lymphatics and veins, in the position of the adult
 +
openings, could be found. The pores, sometimes detected in various
 +
positions, are not adequate to empty the large sacs, and may indeed be
 +
artifacts. Communication with the veins in these stages must be by
 +
osmosis, therefore, and the permanent outlets of the lymphatic system
 +
must develop later.
 +
 +
The left jugular sac in Fig. 8 connects with the thoracic duct, which
 +
arises from a plexus of lymphatics surrounding the aorta. Ventral to the
 +
aorta these vessels receive the lymphatics from the mesentery. There are
 +
none in the leg. The body wall is supplied by those which follow the
 +
external mammary A'ein in its anastomosis with the superficial epigastric,
 +
and by vessels accompanying the ilio-lumbar vein. The ilio-luml)ar vein
 +
of Krause, whicli Hochstetter named the posterior transverse lumbar,
 +
supplies the subcutaneous tissue of the back, and seems to be inversely
 +
homologous with the much larger subscapular vein. At the position
 +
I. n., indicated in Fig. 8, a node is found among the lymphatics accompanying this vein. A similar node exists on the opposite side, and the pair
 +
was identified in a duplicate series of a 20-day rabbit. These superior
 +
inguinal nodes (Krause) develop almost simultaneously with the subscapular nodes already described. The early appearance of the inguinal
 +
nodes further identifies the lymphatics of the ilio-lumbar vein with the
 +
" posterior lymph heart " of Prof. Sabiu. It is my opinion that an
 +
identification of this structure with the amphibian or avian lymph heart
 +
is, at present, not justified. The posterior heart of the bird empties into
 +
the coccygeal veins (Sala), and that of the frog into the transveree iliac
 +
vein, a vessel connecting the femoral with the sciatic vein (Gaupp). The
 +
ilio-lumbar vein is more anterior than either. Its lymphatics do not differ
 +
in form, from those accompanying other veins, and they are presumably
 +
non-contractile. If the first lymph nodes can be utilized in making comparisons, then this " posterior heart " of the rabbit should be compared
 +
with the lymphatics of the subscapular vein, and not with the jugular sac.
 +
The jugular sac itself does not empty into the vertebral vein, like the
 +
anterior heart of the frog. It is non-contractile, so far as known. 1 f it
 +
shall l)e found that the anterior heart of the frog develops from the first
 +
]ym})hatics which are formed in that animal, a comparison between the
 +
jugular sac and a lymph heart may be possible. At present it is not
 +
evident that mammals possess any lymph hearts.
 +
 +
 +
 +
110 'I'lio Dovi'lopinoiit of tlic Lympliatic Systom in liabbits
 +
 +
Summary.
 +
 +
The lymphatic system of rabbits begins along the internal jugular vein
 +
as a detached sac formed by the coalescence of several venous outgrowths.
 +
 +
Similar though smaller sacs arise from the subcardinal and mesenteric
 +
veins at a slightly later date.
 +
 +
Subsequently lymphatic vessels develop along the courses of the azygos
 +
and cutaneous veins, apparently from independent venous outgrowths.
 +
All of these vessels unite with one another to form a continuous system,
 +
which acquires new and permanent openings into the veins near the
 +
subclavian termination.
 +
 +
The first lymph nodes observed are two pairs, one beside the subscapular vessels, and the other beside the ilio-lumbar vessels.
 +
 +
In order to facilitate comparison with Prof. Sabin's work, the following
 +
conclusions may be added :
 +
 +
The lymphatic system does not arise from the venous system by four
 +
outgrowths, but by several. It is not always in communication with the
 +
veins. The outlets of the thoracic and right lymphatic ducts are not persistent primary openings. An identification of mammalian lymph hearts,
 +
comparable with those of the amphibia, should not be made, on the evidence now available. Judged by their relation to the early lymph nodes,
 +
the jugular sac is not comparable with the lymphatics along the iliolumbar vein. However, the study of rabbit embryos confirms the chief
 +
conclusion established by Prof. Sabin, that the lymphatic system is a
 +
derivative of the venous system.
 +
 +
LITERATURE CITED.
 +
 +
Gage, Simon H., 02. — A Reference Handbook of the Medical Sciences. Edited
 +
 +
by Albert H. Buck. 2d ed., Vol. 5, pp. 624-659, New York.
 +
Gaupp, Ernst, gg. — Anatomic des Frosches. 2d ed.. Part 2, Braunschweig.
 +
HocHSTETTER, FERDINAND, g^. — Beitragc zur Entwicklungsgeschichte des
 +
 +
Venensystems der Amnioten, III. Morph. Jahrb., Vol. 20, pp. 543-648.
 +
Krause, W., 68. — Die Anatomic des Kaninchens. Leipzig.
 +
Langeb, C, 68. — Ueber das Lymphgefasssystems des Frosches, III. Sitz.-Ber.
 +
 +
d. Akad. d. Wiss., Wien, Vol. 58, pp. 198-210.
 +
Lewis, Frederic T., 02, — The development of the vena cava inferior. Amer.
 +
 +
Journ. of Anat. Vol, 1, pp. 229-244.
 +
MacCallum, W. G., 02. — Die Beziehung der Lymphgefasse zum Bindegewebe.
 +
 +
Arch. f. Anat. u. Phys., Anat. Abth., pp. 273-291.
 +
Ranvier, L., gj. — Morphologic et developpement des vaisseaux lymphatiques
 +
 +
Chez les mammiferes. Arch. d'Anat. mic. Vol. I, pp. 69-81.
 +
Sabin, Florence R., 02. — On the origin of the lymphatic system .from the
 +
 +
veins and the development of the lymph hearts and thoracic duct in
 +
 +
the pig. Amer. Journ. of Anat., Vol. 1, pp. 367-389.
 +
 +
 +
 +
Frederic T. Lewis 111
 +
 +
Saiun, Florence R., 04. — Onthe development of the superficial lymphatics in
 +
 +
the skin of the pig. Amer. Journ. of Anat., Vol. 3, pp. 183-195.
 +
05. — The development of the lymphatic nodes in the pig, and
 +
 +
theii- relation to the lymph hearts. Amer. Journ. of Anat., Vol. 4,
 +
 +
pp. 355-389.
 +
Sala, LuKii, 00. — Sullo svillupo dei cuori linfatici e dei dotti toracici nell'
 +
 +
embrione di polio. Ric. fatte nel hab. di Anat. norm. d. R. Univ.
 +
 +
di Roma, Vol. 7, pp. 263-296.
 +
Saxer, Fr., 96. — Ueber die Entwickelung und den Bau der normalen Lymph
 +
driisen und die Entstehung der roten und weissen Blutkorperchen.
 +
 +
Anat. Hefte, Abt. 1, Vol. 6, pp. 349-532.
 +
 +
 +
 +
THE DEVELOPMENT OF THE VEINS IN THE LIMBS OF
 +
BABBIT EMBEYOS.
 +
 +
BY
 +
 +
FREDERIC T. LEWIS, A. M., M. D.
 +
 +
From the Emhryological Laboratory, Harvard Medical School.
 +
 +
With 1 Text Figure.
 +
 +
In connection with the preceding study of the lymphatic system it was
 +
necessary to reconstruct the veins of the shoulder and hip in a series of
 +
rabbit embrj^os. The reconstructions were then extended to include the
 +
distal portions of these vessels, complete figures of which had never been
 +
published. Hochstetter, in 1891, had observed the veins in the limbs of
 +
living rabbit embryos, and had studied them in serial sections. His
 +
drawings, however, show only detached portions of the veins such as
 +
could be seen under most favorable conditions, in living embryos. Ten
 +
years later Grosser described but did not reconstruct, the developing veins
 +
in the extremities of bats. To these two investigators embryology is indebted for the present knowledge of the veins in mammalian limbs. It
 +
is proposed to review their work, while describing the reconstructions,
 +
considering first the veins of the anterior extremity, then those of the
 +
posterior extremity, and finally the homologies which exist between the
 +
two sets.
 +
 +
A^EINS OF THE ANTERIOR EXTREMlTY.
 +
 +
In the youngest rabbit figured, an embryo of 13 days. Fig. 1, p. 97,
 +
the small vessels along the radial or anterior border of the arm unite to
 +
form a vein which follows the periphery of the limb to its posterior or
 +
ulnar border, and then ascends behind the brachial plexus to terminate
 +
near the junction of the anterior and posterior cardinal veins. It receives
 +
a branch which at this stage is not well defined, ascending in the body
 +
wall. This is the Seitenrumpfvene of Hochstetter, and becomes the
 +
external mammary vein of the adult.
 +
 +
According to Hochstetter, in rabbits of 12 and 121/2 days, the " border
 +
vein " mak€s a complete circuit of the limb, and its radial part either
 +
empties into the ulnar vein near its termination or connects with the
 +
cardinal vein directly. But this radial vein is said to be hard to follow
 +
 +
American Jocrxal of Anatomy. — Vol. V.
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8
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114 I)evc4opment of the A'oiiis in the Limbs of Eabbit Embrvos
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because *" attended by several venous twigs of nearly tht' same caliber,
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and only shortly before its termination is it recognizahU' as a distinct
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(starkeres) vessel " (p. 24). In a 13-day rabbit the radial vein had disappeared, " since it had been but imperfectly marked out." Similarly
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(irosser found a radial vein emptying into the anterior cardinal close to
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the ulnar vein, in the youngest bat which he studied (4% mm.). In the
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next stage (614 nim.) it had vanished (p. 136). An examination of
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rabbits of 12 and I2I/2 days, together with younger ones in the Harvard ■
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Collection, shows that the first vein of the arm develops along its ulnar
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margin, extending distally around the border to the radial side. Small
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and variable vessels such as Hochstetter described as a radial vein may
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occur, as shown in Fig. 1, p. 97, Init they do not form a structure comjDarable with the primitive ulnar vein. The latter may be called the
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primary vein of the arm.
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The rabbit of 14 days. Fig. 2, p. 98, presents the condition described
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by Hochstetter in embrvos of 13 days. The primitive ulnar vein has
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acquired a new outlet ventral to the brachial plexus, so that, by the persistence of the original dorsal termination, most of the plexus and the
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brachial artery are surrounded by a loop of vein. In the following rabbit, Fig. 3, p. 100, the ventral outlet of the idnar vein is the chief one.
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This specimen shows a small vessel extending from the external jugular
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vein toward the radial border of the arm.
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The next embryo, Fig. 4, p. 102, is considerably more advanced. The
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dorsum of the hand, which was previously its external surface has rotated
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and become anterior; the arm is in pronation. The differentiation of the
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fingers is indicated by the sinuous terminal border of the hand, and by
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shallow interdigital depressions on its dorsum. Beneath these, interdigital veins have been formed, probably from branches of the primitive
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ulnar vein. A new vein has grown from the external jugular down the
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anterior or radial border of the arm, and has united with the independently formed interdigital veins. This is the cephalic vein of the adult.
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It is embryologically the second vein of the arm.
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Hochstetter states that the cephalic vein in rabbits develops toward the
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body from the back of the hand, connecting with the ulnar vein at the
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elbow, and later continuing up the arm to the external jugular vein.
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The preceding reconstructions of the rabbit agree better with Grosser's
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description of the bats. He failed to find a stage in which the cephalic
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vein emptied into the ulnar. In the earliest specimen in which the
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cephalic vein was found, it connected with the external jugular vein.
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The cephalic vein of the 17-day rabbit is the chief vein of the limb, and
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has developed a In-aneh whicli follows the radial artery, the deej) radial
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Frederic T. Lewis 115
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vein, Fig. T, }>. 107. At 20 days, Fig. 8, j). 108, the eeplialic vein has
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acquired its new and permanent orifice near the axiUary vein. The
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jugulo-cephalic vein niarl-cs its former outlet.
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Witli tlie differentiation of the digits, the primitive ulnar veiii becomes
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greatly reduced by the loss of its distal portion. This is shown in Fig.
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•1. At 17 days, Fig. 7, p. 107, the continuity of the primitive ulnar vein
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has been interrupted at tlie elbow, resulting in further reduction. The
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vein then extends from the elbow to the superior vena cava, following the
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brachial artery, from around which it receives small branches. In tlie
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20-day embryo, Fig. 8, p. 108, the brachial vein (proximal part of the
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primitive ulnar) is continued down the forearm following the ulnar
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artery. If we may judge from the position of this vessel, there lias been
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a re-establishment of the course which was interrupted in the younger
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embryo. Hochstetter, however, states (p. 28) that in rabbits the forearm section of the primitive ulnar vein seems to disappear, although in
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man (p. 33) the corresponding vessel is preserved throughout, and forms
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the basilic vein of the forearm and arm, the axillary and subclavian veins.
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The question arises whether the primitive ulnar vein should be described as producing the deep ulnar, brachial, and axillary veins, naming
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it for the adjacent arteries, or as forming the basilic and axillary veins,
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considering the cutaneous vein of the corresponding region as its more
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direct derivative. This uncertainty calls attention to the fact that both
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the superficial and deep sets of veins have a common origin, and that before their separation the embryonic vein may properly be called either
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brachial or l^asilic. The ra])I)it of 20 days is characterized by the establishment of this brachial (or l:)asilic) vein.
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In the develo])ment of the veins of the arm three stages have been distinguished :
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1st. The stage of the primitive ulnar vein.
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2 d. " " " " cephalic vein.
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3d. " " " " brachial vein, the cephalic vein persisting.
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Veins of the Posterior Extremity.
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A rabbit of IOI/2 days (Harvard Collection, No. 199) has a very large
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umbilical vein which sends l^ranches into both Jimbs. Those in the leg
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form a net which connects with the posterior cardinal vein, still a minute
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vessel in the caudal end of the l)ody. From the network a vein is developed, which after following the periphery of the limb and passing along
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its posterior or fibular l)order, empties into the cardinal vein. This vessel
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may be called tlie ])rimitive fibular vein. The original connections of the
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116 Developmeut of tlie Veins in the Limbs of Eabbit Embryos
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net with the umbilical vein do not form ;i well defined vessel and soon
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disappear. Although Ilochstetter recognizes this, he refers to the connection with the umbilical vein as a tibial border vein. Grosser could not
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identify such a vessel in any of his three youngest bats (p. 149).
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The primitive fibular vein as shown in Fig. 2, p. 98, is a vessel readily comparable with the primitive ulnar vein. Both course along the
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posterior borders of their respective limbs, in which they are the first
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veins developed. They are undoubtedly homologous. In later development, however, they constantly diverge from one another. Even at 14
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days the fibular vein has two small branches which are not matched by
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any belonging to the ulnar vein. One of these, coming from twigs on
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the outer and caudal surface of the leg, becomes the anterior tibial vein,
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An. T. The other which extends mediad toward what at this stage is
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the inguinal line, may be referred to as the " connecting branch," c. b.
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In the more advanced embrj^o. Fig. 3, p. 100, the same branches appear
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in similar relations. They have become much larger at 14 days 18
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hours. Fig. 4, p. 102. Here the anterior tibial branch has extended
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diagonally down the limb to the dorsum of the foot. The connecting
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branch has sent its twigs into the abdominal wall and the adjoining tibial
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border of the limb. The primitive fibular vein is still the chief vein of
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the leg.
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In the older rabbit, Fig. 7, p. lOT, the difl'erentiation of the toes has
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broken up the distal portion of the primitive fibular vein, wdiich has disappeared almost to the point where it receives its anterior tibial branch.
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This branch now arises from the interdigital veins on the dorsum of the
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foot and its main trunk appears continuous with the proximal part of the
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primitive fibular vein. The anterior tibial and primitive fibular veins
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together, now constitute the sciatic vein, wliich is embryologically the
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second vein of the leg.
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The reconstructions to wliich we have referred agree with Hochstetter's
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description of the development of the sciatic vein except in one detail.
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They do not show that a part of the primitive fibular vein distal to the
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anterior tibial branch persists as the small saphenous vein.
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In the rabbit of 14 days 18 hours, a third vein of the leg has begun its
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development. This is the femoral vein which terminates in the posterior
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cardinal anterior to the sciatic vein. It advances toward the tibial l^order
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of the limb. At 17 days, Fig. 7, p. 107, it is seen approaching the external mammary and the connecting branch of the sciatic vein. In the
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embryo of 20 days, Fig. 8, p. 108, it has anastomosed with both and passes
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down the leg as the posterior tibial vein, Po. T.
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Just as it is questionable in the arm whether the parent vessel should
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Frederic T. Lewis 117
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be designated hriu-liial or basilic, so in tbe leg there is the cboice between
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femoral and large saphenous vein. Both of the latter spring from the
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vessel which we have called femoral. The close relation between the two
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is shown by Krause's description of the veins in the adult rabbit, where the
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posterior tibial is considered to be the distal continuation of the large
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saphenous vein. It seems probable also, that the anteror tibial vein,
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which is quite superficial at 20 days, though it accompanies the artery,
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should give rise to the small saphenous vein, with which it anastomoses
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in the adult. Hochstetter, as already noted, assigns a somewhat different
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origin to the small saphenous vein.
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The condition found in the rabbit at 20 days, is essentially that of the
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adult. The sciatic vein remains a large vessel. In man, assuming that
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the embryological history is similar to that of the rabbit, the proximal
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section of the sciatic vein dwindles after the formation of the femoral
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anastomosis near the knee. The sciatic vein is represented, therefore,
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merely by the collateral circulation of the thigh, as figured by Charpy
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(Poirier's Anatomic, Vol. 2, p. 1052), and by Spalteholz (Handatlas,
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Vol. 2, p. 469).
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The preceding observations seem to establish three stages in the venous
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development of the leg, comparable with those in the arm.
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1st. The stage of the primitive fibular vein.
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2d. " " " " sciatic vein.
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3d. " " " " femoral vein, the sciatic vein persisting (in
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man, very much reduced ) .
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Homologies between the Veins of the Anterior and Posterior
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Extremities.
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Bardeleben's view that the primary vein of the arm consisted of the
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vena cephalica antibrachii, vena mediana cubiti, and vena basilica brachii,
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and that this was homologous with the vena saphena magna of the leg
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was rightfully criticized and condemned by Hochstetter. Nevertheless it
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is referred to somewhat favorably by Charpy.
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Krause finds that the cephalic and sciatic veins are analogous (p. 210).
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Hochstetter denies this, and arrives at the following conclusions. Since
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the ulnar and fibular borders of the limbs are homologous, the primitive
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veins which follow them are also homologous. The small saphenous vein
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and the basilic vein of the forearm, being presumably persistent portions
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of the primitive veins, are therefore homologous. The cephalic and large
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saphenous veins are secondary formations, and any comparison between
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118 l)ov('l()|)nuMit of the Veins in the Limbs of Eabbit Embryos
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them is uncertain. The femoral and brachial veins " sliow no a<ii-eement
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either in position or in origin " (p. ;}-")).
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These conclusions clearly depend upon the serial homology of the limbs.
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If we should accept the idea of inverse homology, advocated by Wilder,
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WynuTn, and others, according to whom the thumb is comparable with the
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little toe, and the radial border with the ulnar, then conclusions almost the
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reverse of Hochstetter's would be expected. A third basis for comparison
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