ANAT2341 Lab 9: Difference between revisions

Latest revision as of 10:13, 11 October 2018

1. Project Peer Assessment

No Quiz this week after mid-semester break.

Complete the peer assessment exercise for your individual assessment this week. Write a brief balanced feedback for each project (other than your own). Paste this feedback on each group discussion page as well as your own student page, where I will provide feedback.

Below are the criteria used by the course coordinator for the final project assessment.

Group Assessment Criteria
Science Student Projects The key points relating to the topic that your group allocated are clearly described. The choice of content, headings and sub-headings, diagrams, tables, graphs show a good understanding of the topic area. Content is correctly cited and referenced. The wiki has an element of teaching at a peer level using the student's own innovative diagrams, tables or figures and/or using interesting examples or explanations. Evidence of significant research relating to basic and applied sciences that goes beyond the formal teaching activities. Relates the topic and content of the Wiki entry to learning aims of embryology. Clearly reflects on editing/feedback from group peers and articulates how the Wiki could be improved (or not) based on peer comments/feedback. Demonstrates an ability to review own work when criticised in an open edited wiki format. Reflects on what was learned from the process of editing a peer's wiki. Evaluates own performance and that of group peers to give a rounded summary of this wiki process in terms of group effort and achievement. The content of the wiki should demonstrate to the reader that your group has researched adequately on this topic and covered the key areas necessary to inform your peers in their learning. Develops and edits the wiki entries in accordance with the above guidelines.
More Information on Assessment Criteria \| Science Student Projects

2. Guest Lecturer - Dr Gonzalo Del Monte

Lecture Slides

References

Search PubMed: Del+Monte G

Del Monte G & Harvey RP. (2012). An endothelial contribution to coronary vessels. Cell , 151, 932-4. PMID: 23178115 DOI.

del Monte G, Casanova JC, Guadix JA, MacGrogan D, Burch JB, Pérez-Pomares JM & de la Pompa JL. (2011). Differential Notch signaling in the epicardium is required for cardiac inflow development and coronary vessel morphogenesis. Circ. Res. , 108, 824-36. PMID: 21311046 DOI.

Luna-Zurita L, Prados B, Grego-Bessa J, Luxán G, del Monte G, Benguría A, Adams RH, Pérez-Pomares JM & de la Pompa JL. (2010). Integration of a Notch-dependent mesenchymal gene program and Bmp2-driven cell invasiveness regulates murine cardiac valve formation. J. Clin. Invest. , 120, 3493-507. PMID: 20890042 DOI.

Chandrakanthan V, Yeola A, Kwan JC, Oliver RA, Qiao Q, Kang YC, Zarzour P, Beck D, Boelen L, Unnikrishnan A, Villanueva JE, Nunez AC, Knezevic K, Palu C, Nasrallah R, Carnell M, Macmillan A, Whan R, Yu Y, Hardy P, Grey ST, Gladbach A, Delerue F, Ittner L, Mobbs R, Walkley CR, Purton LE, Ward RL, Wong JW, Hesson LB, Walsh W & Pimanda JE. (2016). PDGF-AB and 5-Azacytidine induce conversion of somatic cells into tissue-regenerative multipotent stem cells. Proc. Natl. Acad. Sci. U.S.A. , 113, E2306-15. PMID: 27044077 DOI.

Huang Y, Thoms JA, Tursky ML, Knezevic K, Beck D, Chandrakanthan V, Suryani S, Olivier J, Boulton A, Glaros EN, Thomas SR, Lock RB, MacKenzie KL, Bushweller JH, Wong JW & Pimanda JE. (2016). MAPK/ERK2 phosphorylates ERG at serine 283 in leukemic cells and promotes stem cell signatures and cell proliferation. Leukemia , 30, 1552-61. PMID: 27055868 DOI.

@@ Line 1: / Line 1: @@
-=Muscle Development =
+==1. Project Peer Assessment==
-[[File:Actin_myosin_crossbridge_3D_animation.gif]] [[File:Actin_myosin_crossbridge_3D_animation.jpg]][[File:Muscle fiber types.jpg]]
-== Introduction ==
+No Quiz this week after mid-semester break.
-This laboratory concerns the development and differentiation of skeletal muscle, muscle stem cells, hypertrophy, fibre type differentiation and plasticity.
-==Objectives==
+Complete the peer assessment exercise for your individual assessment this week. Write a brief balanced feedback for each project (other than your own). Paste this feedback on each group discussion page as well as your own student page, where I will provide feedback.
-# Understand the origin, differentiation and development of skeletal muscle tissue.
-# Know what is meant by patterning, conversion and adult plasticity of muscle fibre type.
-# Develop an understanding of research methods for studying skeletal muscle development and function.
-[[File:Sarcomere_animation.gif]]
+Below are the criteria used by the course coordinator for the final project assessment.
+{{Group Assessment Criteria table}}
-[http://www.lab.anhb.uwa.edu.au/mb140/CorePages/Muscle/Muscle.htm#SKELETAL Skeletal Muscle Histology]
+==2. Guest Lecturer - Dr Gonzalo Del Monte==
-== Terms ==
+[[Media:Lecture Heart Development UNSW 2-10-18 .pdf|'''Lecture Slides''']]
-'''anterior tibialis''' - (tibialis anterior) skeletal muscle situated on the lateral side of the tibia and is a direct flexor of the foot at the ankle-joint.
-'''cis-acting elements''' -  DNA sequences that through transcription factors or other trans-acting elements or factors, regulate the expression of genes on the same chromosome.
-'''enhancer''' - A cis-regulatory sequence that can regulate levels of transcription from an adjacent promoter. Many tissue-specific enhancers can determine spatial patterns of gene expression in higher eukaryotes. Enhancers can act on promoters over many tens of kilobases of DNA and can be 5' or 3' to the promoter they regulate.
-'''extensor digitorum longus'''  - (EDL) skeletal muscle situated at the lateral part of the front of the leg and extend the phalanges of the toes, and, continuing their action, flex the foot upon the leg.
+===References===
+Search PubMed: [https://www.ncbi.nlm.nih.gov/pubmed/?term=Del+Monte+G%5BAuthor%5D Del+Monte G]
-'''Gtf2ird1''' - General Transcription Factor 2 -I Repeat domain-containing protein 1.  [http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=604318 OMIM: Gtf2ird1]
-'''MusTRD1'''  - muscle TFII-I repeat domain-containing protein 1.
+{{#pmid:23178115}}
-'''MyHC''' - acronym for myosin heavy chain.
+{{#pmid:21311046}}
-'''myoblast''' - the undifferentiated mononucleated muscle cell progenitor, which in skeletal muscle fuses to form a myotube, that in turn expresses contractile proteins to form a muscle fibre.
+{{#pmid:20890042}}
-'''myosin heavy chain''' - protein forming the thick filament of the sarcomere and the motor for actin-myosin contraction. There are 17 different myosin classes.
+{{#pmid:27044077}}
-'''myotube''' - the initial multinucleated cell formed by fusion of myoblasts during skeletal muscle development.
+{{#pmid:27055868}}
-'''promoter''' - A regulatory region a short distance upstream from the 5' end of a transcription start site that acts as the binding site for RNA polymerase II. A region of DNA to which RNA polymerase IIbinds in order to initiate transcription.
-'''regulatory sequence''' - (regulatory region, regulatory area) is a segment of DNA where regulatory proteins such as transcription factors bind preferentially.
-'''soleus''' - skeletal muscle situated immediately in front of the gastrocnemius and when standing taking its fixed point from below, steadies the leg upon the foot and prevents the body from falling forward.
-'''Troponin''' - striated muscle contraction is regulated by the calcium-ion-sensitive, multiprotein complex troponin and the fibrous protein tropomyosin. Troponin has 3 subunits (TnC, TnI, TnT) and is located on the actin filament. [http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=191040 OMIM: Troponin I slow]
+{{2018ANAT2341}}
-'''visuospatial deficiency''' -  performing the Delis hierarchical processing task, subjects are asked to copy a large global figure made of smaller local forms. Both Down syndrome (DS) and William-Beuren syndrome (WBS) groups fail but in different ways. WBS individuals produce the local elements and DS individuals produce only the global forms. [http://www.ncbi.nlm.nih.gov/pubmed/12952863?dopt=Abstract PMID: 12952863] [http://hmg.oxfordjournals.org/cgi/content/full/12/suppl_2/R229#DDG299F2 Hum Mol Genet.]
-'''William-Beuren syndrome''' - (WBS) rare developmental disorder (1/20,000–1/50,000 live births). A contiguous gene deletion syndrome resulting from the hemizygous deletion of several genes on chromosome [http://www.ncbi.nlm.nih.gov/Omim/getmap.cgi?l194050 7q11.23]. The syndrome has associated craniofacial abnormalities, hypersociability and visuospatial defects. [http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=194050 OMIM: William-Beuren syndrome]
-===Search ===
-* '''Bookshelf''' [http://www.ncbi.nlm.nih.gov/sites/entrez?db=Books&cmd=search&term=muscle_development muscle development] |  [http://www.ncbi.nlm.nih.gov/sites/entrez?db=Books&cmd=search&term=muscle_fiber_type muscle fiber type] | [http://www.ncbi.nlm.nih.gov/sites/entrez?db=Books&cmd=search&term=troponin troponin]
-* '''Pubmed''' [http://www.ncbi.nlm.nih.gov/sites/gquery?itool=toolbar&cmd=search&term=muscle_fiber_type muscle fiber type] | [http://www.ncbi.nlm.nih.gov/sites/gquery?itool=toolbar&cmd=search&term=troponin troponin] | [http://www.ncbi.nlm.nih.gov/sites/gquery?itool=toolbar&cmd=search&term=Gtf2ird1 Gtf2ird1] |
-==UNSW Embryology Links==
-* [[File:Muscle_development_1-Talk1_muscle_development.pdf‎]]
-* [[File:Talk1_muscle_development_4_per_page.pdf‎‎]]
-* [[File:Part_2_Muscle_Development.pdf‎]]
-* [[File:Part_2_Muscle_Development_4_per_page.pdf]]‎
-== External Links ==
-{{External Links}}
-* Blue Histology [http://www.lab.anhb.uwa.edu.au/mb140/CorePages/Muscle/Muscle.htm#SKELETAL Skeletal Muscle histology]
-* Primer [http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.0020348 Skeletal Muscle Fiber Type: Influence on Contractile and Metabolic Properties]
-* Gray's Anatomy of the Human Body - [http://www.bartleby.com/107/129.html The Muscles and Fasciæ of the Leg] | [http://www.bartleby.com/107/illus440.html Cross-section through middle of leg]
-* Wiki [http://en.wikipedia.org/wiki/Skeletal_muscle Skeletal muscle] | [http://en.wikipedia.org/wiki/Troponin Troponin]
-* [http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=604318 OMIM: Gtf2ird1]
-* [http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=194050 OMIM: William-Beuren syndrome]
-==Individual Assessment==
-* Each student should now look at each of the other Group projects in the class.
-* Next prepare a critical assessment (should include both positive and negative issues) of each project using the project group assessment criteria.
-* This assessment should be pasted without signature on the top of the specific project's discussion page. (minimum length 3-5 paragraphs/project)
-* This critical assessment should also be pasted on your own student page.
-* Each student should therefore have 5 separate reports pasted on their own page for this assessment item.
-* Length, quality and accuracy of your reports will be part of the overall mark for this assessment.
-** there will be a greater loading on this than simple question assessments.
-{| class="wikitable mw-collapsible mw-collapsed"
-! Group Assessment Criteria
-|-
-| [[File:Mark_Hill.jpg|50px]]
-# The key points relating to the topic that your group allocated are clearly described.
-# The choice of content, headings and sub-headings, diagrams, tables, graphs show a good understanding of the topic area.
-# Content is correctly cited and referenced.
-# The wiki has an element of teaching at a peer level using the student's own innovative diagrams, tables or figures and/or using interesting examples or explanations.
-# Evidence of significant research relating to basic and applied sciences that goes beyond the formal teaching activities.
-# Relates the topic and content of the Wiki entry to learning aims of embryology.
-# Clearly reflects on editing/feedback from group peers and articulates how the Wiki could be improved (or not) based on peer comments/feedback. Demonstrates an ability to review own work when criticised in an open edited wiki format. Reflects on what was learned from the process of editing a peer's wiki.
-# Evaluates own performance and that of group peers to give a rounded summary of this wiki process in terms of group effort and achievement.
-# The content of the wiki should demonstrate to the reader that your group has researched adequately on this topic and covered the key areas necessary to inform your peers in their learning.
-# Develops and edits the wiki entries in accordance with the above guidelines.
-|}
-==Group Project==
-* Peer Assessment of projects.
-===Plagiarism===
-Currently all students originally assigned to each group are listed as equal authors/contributors to their project. If you have not contributed the content you had originally agreed to, nor participated in the group work process, then you should contact the course coordinator immediately and either discuss your contribution or request removal from the group author list. Remember that all student online contributions are recorded by date, time and the actual contributed content. A similar email reminder will be sent to all current students.
-Please note the [https://my.unsw.edu.au/student/atoz/Plagiarism.html Universities Policy regarding Plagiarism]
-In particular this example:
-:"Claiming credit for a proportion of work contributed to a group assessment item that is greater than that actually contributed;"
-Academic Misconduct carries penalties. If a student is found guilty of academic misconduct, the penalties include warnings, remedial educative action, being failed in an assignment or excluded from the University for two years.
-----
-==SOMS Summer Research Awards==
-* The Research Support Committee is pleased to announce that it will again be offering Summer Research Awards (SRAs).
-* These awards are for undergraduate students carrying out supervised research in SOMS between November 2014 and February 2015.
-* Successful students will receive a stipend each week for 6 weeks of research. [http://medicalsciences.med.unsw.edu.au/students/undergraduate/summer-research-awards More Information?]
-==References==
-<references/>
-{{2014ANAT2341}}