ANAT2341 Lab 8: Difference between revisions

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==Organogenesis Lab==
'''PRACTICAL CLASS PROGRAM:'''
* Weekly Quiz + revision (10 minutes)
* Completion of surveys (10 minutes)
* Practical class activities (40 minutes)
* Guest Lecture by A/Prof Stuart Fraser (45 minutes)
* Practical Class Revision (15 minutes)


== 1. QUIZ ==


== 2. Organogenesis Lab ==
'''PRACTICAL CLASS ACTIVITIES''' (40 minutes):
In this lab you will dissect fertile chicken eggs and study fixed mouse embryos up to mid-gestation using dissection microscopes. You will name the embryonic anatomical structures, and describe what these will give rise to.
* Cell lineage activity
* Investigate the chicken embryo skeletal preps


[[Media:ANAT2341_-_INTRODUCTION_TO_THE_CHICK_EMBRYO.pdf|Organogensis Lab Manual]]


[[File:Chicken_Embryo_Hamburger_stages.jpg|600px|link=Hamburger Hamilton Stages]]
'''LEARNING OBJECTIVES''':
* Understanding organogenesis
* Understanding the developmental paths of cell types/structures
* Understand the developmental basis of human disease
* Understanding skeletal development
* Understanding blood cell development
* Understanding research into blood cell development


''These are the Hamburger stages of chicken development''


See also:
<pubmed>18989413</pubmed>


J. Korn, M., S. Cramer, K. Windowing Chicken Eggs for Developmental Studies. J. Vis. Exp. (8), e306, doi:10.3791/306 (2007).
'''Blood Cell Development - A/Prof Stuart Fraser'''
<br><br>
Biographical details


Stuart Fraser joined the Discipline of Physiology as Sesquicentenial lecturer in Molecular Embryology in April 2010. Prior to returning to Australia, Dr. Fraser was Assistant Professor in Hematology/Medical Oncology in the Mount Sinai School of Medicine in New York City for 6 years. Dr. Fraser also completed postdoctoral studies at the University of Mainz in Germany and spent 4 years at Kyoto University in Japan.
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More about chicken embryogenesis: [[Chicken Development]] | [[Hamburger Hamilton Stages]]
Stuart's main research interests focus upon the mechanisms controlling the formation of the {{blood}}, or hematopoietic lineages, in the embryo and how these processes can go awry in the adult.


==References==
{{#pmid:31273739}}


<gallery>
{{#pmid:29076088}}
File:HHstage1-4.jpg|stage 1-4
File:HHstage5-10.jpg|stages 5-10
File:HHstage11-14.jpg|stages 11-14
File:HHstage15-18.jpg|stages 15-18
File:HHstage19-21.jpg|stages 19-21
File:HHstage22-25.jpg|stages 22-25
File:HHstage26-28.jpg|stages 26-28
File:HHstage29-32.jpg|stage 29-32
</gallery>


[[File:Mouse_vs_Human_embryogenesis.jpg]]
{{#pmid:28401096}}


''This figure compares the human and mouse developmental stages''
{{#pmid:28395744}}


More about Mouse embryogenesis: [[Mouse Timeline Detailed]]
{{#pmid:26898901}}


{{#pmid:26113865}}


{{Chicken}}
Search PubMed: [https://www.ncbi.nlm.nih.gov/pubmed/?term=Fraser%20ST%5BAuthor%5D&cauthor=true&cauthor_uid=31273739 Fraser ST]
 
===External Links===
{{External Links}}
 
* JOVE - [http://www.jove.com/science-education/5153/an-introduction-to-the-chick-gallus-gallus-domesticus An Introduction to the Chicken]
 
 
{{2017ANAT2341 footer}}

Latest revision as of 16:37, 8 November 2019

PRACTICAL CLASS PROGRAM:

  • Weekly Quiz + revision (10 minutes)
  • Completion of surveys (10 minutes)
  • Practical class activities (40 minutes)
  • Guest Lecture by A/Prof Stuart Fraser (45 minutes)
  • Practical Class Revision (15 minutes)


PRACTICAL CLASS ACTIVITIES (40 minutes):

  • Cell lineage activity
  • Investigate the chicken embryo skeletal preps


LEARNING OBJECTIVES:

  • Understanding organogenesis
  • Understanding the developmental paths of cell types/structures
  • Understand the developmental basis of human disease
  • Understanding skeletal development
  • Understanding blood cell development
  • Understanding research into blood cell development


Blood Cell Development - A/Prof Stuart Fraser

Biographical details

Stuart Fraser joined the Discipline of Physiology as Sesquicentenial lecturer in Molecular Embryology in April 2010. Prior to returning to Australia, Dr. Fraser was Assistant Professor in Hematology/Medical Oncology in the Mount Sinai School of Medicine in New York City for 6 years. Dr. Fraser also completed postdoctoral studies at the University of Mainz in Germany and spent 4 years at Kyoto University in Japan. Back to Top

Stuart's main research interests focus upon the mechanisms controlling the formation of the blood, or hematopoietic lineages, in the embryo and how these processes can go awry in the adult.

References

Colonne CK, Yeo JH, McKenzie CV & Fraser ST. (2019). Identification and Analysis of Mouse Erythroid Progenitor Cells. Methods Mol. Biol. , 2029, 125-145. PMID: 31273739 DOI.

Yeo JH, Cosgriff MP & Fraser ST. (2018). Analyzing the Formation, Morphology, and Integrity of Erythroblastic Islands. Methods Mol. Biol. , 1698, 133-152. PMID: 29076088 DOI.

Yumine A, Fraser ST & Sugiyama D. (2017). Regulation of the embryonic erythropoietic niche: a future perspective. Blood Res , 52, 10-17. PMID: 28401096 DOI.

Ross SB, Fraser ST, Bagnall RD & Semsarian C. (2017). Peripheral blood derived induced pluripotent stem cells (iPSCs) from a female with familial hypertrophic cardiomyopathy. Stem Cell Res , 20, 76-79. PMID: 28395744 DOI.

Yeo JH, McAllan BM & Fraser ST. (2016). Scanning Electron Microscopy Reveals Two Distinct Classes of Erythroblastic Island Isolated from Adult Mammalian Bone Marrow. Microsc. Microanal. , 22, 368-78. PMID: 26898901 DOI.

Al-Drees MA, Yeo JH, Boumelhem BB, Antas VI, Brigden KW, Colonne CK & Fraser ST. (2015). Making Blood: The Haematopoietic Niche throughout Ontogeny. Stem Cells Int , 2015, 571893. PMID: 26113865 DOI.

Search PubMed: Fraser ST