Difference between revisions of "ANAT2341 Lab 2"

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=Week 1 to 3 Development=
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PRACTICAL CLASS PROGRAM:
{{ANAT2341Lab2}}
 
  
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* Weekly Quiz + revision (15 minutes)
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* Practical class activities: (45 minutes)
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* Guest Lecture by [https://wch.med.unsw.edu.au/people/associate-professor-robert-gilchrist Professor Robert Gilchrist] (45 minutes)
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* Practical Class Revision (15 minutes)
  
==Oocyte Biology Research Unit==
 
[[File:Oocyte BMP15 and GDF9 effects.jpg|thumb|Oocyte BMP15 and GDF9 effects PMID 25058588]]
 
{|
 
|-bgcolor="FAF5FF"
 
! Associate Professor Robert Gilchrist
 
|-
 
| Talk - The Reproductive Technology Revolution
 
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| Dr Gilchrist’s primary research interests are in the regulation of mammalian oocyte development and maturation, and the development of novel oocyte maturation techniques for infertility treatment.
 
|-bgcolor="FAF5FF"
 
| [https://research.unsw.edu.au/people/associate-professor-robert-bruce-gilchrist UNSW Research Gateway] - [http://www.ncbi.nlm.nih.gov/pubmed/?term=Gilchrist+R%5BAuthor%5D PubMed]
 
|-
 
|}
 
  
===Recent Article===
 
  
<pubmed>26254468</pubmed>
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PRACTICAL CLASS ACTIVITIES:
  
:"Growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) are oocyte-specific growth factors with central roles in mammalian reproduction, regulating species-specific fecundity, ovarian follicular somatic cell differentiation and oocyte quality. In the human, GDF9 is produced in a latent form, the mechanism of activation being an open question. Here, we produced a range of recombinant GDF9 and BMP15 variants, examined their in silico and physical interactions, and their effects on ovarian [[Granulosa Cells|granulosa cells]] (GC) and oocytes. We found that the potent synergistic actions of GDF9 and BMP15 on GC can be attributed to the formation of a heterodimer, which we have termed cumulin. Structural modelling of cumulin revealed a dimerization interface identical to homodimeric GDF9 and BMP15, indicating likely formation of a stable complex. This was confirmed by generation of recombinant heterodimeric complexes of pro/mature domains (pro-cumulin) and covalent mature domains (cumulin). Both pro-cumulin and cumulin exhibited highly potent bioactivity on GC, activating both SMAD2/3 and SMAD1/5/8 signaling pathways, and promoting proliferation and expression of a set of genes associated with oocyte-regulated GC differentiation. Cumulin was more potent than pro-cumulin, pro-GDF9, pro-BMP15 or the two combined on GC. However, on cumulus-oocyte complexes, pro-cumulin was more effective than all other growth factors at notably improving oocyte quality as assessed by subsequent day 7 embryo development. Our results support a model of activation for human GDF9 dependant on cumulin formation through heterodimerization with BMP15. Oocyte-secreted cumulin is likely to be a central regulator of fertility in mono-ovular mammals."
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# Virtual human embryo dissections and histology
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# Embryo models and specimens of human developmental abnormalities (optional)
  
  
:'''OMIM  Links:''' [http://omim.org/entry/601918 GDF9] | [http://omim.org/entry/300247 BMP15]  | [http://omim.org/entry/601366 SMAD2]
 
  
==Objectives==
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LEARNING OBJECTIVES:
  
# Identify the key features of fertilization.
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* Understanding of reproductive technologies
# Identify the key features of week 1 development.
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* Understanding of events during early ectoderm and mesoderm development
# Identify the key features of week 2 development.
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* Understanding of the process of neurulation and neural crest formation
# Complete the individual assessment 2.
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* Understanding of the adult components derived from ectoderm and mesoderm
# Group assessment project.
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* Understanding the process of body cavity formation
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* Brief understanding of early heart formation
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* Brief understanding of abnormalities associated with early ectoderm and mesoderm development
  
==Fertilization Reviews==
 
  
# [http://www.ncbi.nlm.nih.gov/pubmed/18649281 Review of sperm penetration through cumulus mass and zona pellucida]
 
# [http://www.ncbi.nlm.nih.gov/pubmed/22054237 Review of sperm-egg interaction]
 
# [http://www.ncbi.nlm.nih.gov/pubmed/22217572 Review of parthenogenesis]
 
  
==Human development timeline==
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PRACTICAL CLASS NOTES:
  
[[File:Human development timeline graph 01.jpg|600px]]
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The Practical class notes are available on [https://moodle.telt.unsw.edu.au/mod/url/view.php?id=2398854 Moodle].
 
 
 
 
 
 
 
 
{{ANAT2341Lab2}}
 
 
 
 
 
 
 
 
 
 
 
 
 
{{2015ANAT2341}}
 

Latest revision as of 11:41, 26 September 2019

PRACTICAL CLASS PROGRAM:

  • Weekly Quiz + revision (15 minutes)
  • Practical class activities: (45 minutes)
  • Guest Lecture by Professor Robert Gilchrist (45 minutes)
  • Practical Class Revision (15 minutes)


PRACTICAL CLASS ACTIVITIES:

  1. Virtual human embryo dissections and histology
  2. Embryo models and specimens of human developmental abnormalities (optional)


LEARNING OBJECTIVES:

  • Understanding of reproductive technologies
  • Understanding of events during early ectoderm and mesoderm development
  • Understanding of the process of neurulation and neural crest formation
  • Understanding of the adult components derived from ectoderm and mesoderm
  • Understanding the process of body cavity formation
  • Brief understanding of early heart formation
  • Brief understanding of abnormalities associated with early ectoderm and mesoderm development


PRACTICAL CLASS NOTES:

The Practical class notes are available on Moodle.