ANAT2341 Lab 2: Difference between revisions

From Embryology
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| [[File:Manipulating_the_Mouse_Embryo.jpg|200px]]
| [[File:Manipulating_the_Mouse_Embryo.jpg|200px]]
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| [http://cshlpress.com/default.tpl?cart=147944968860031853&action=full&--eqskudatarq=982 Manipulating the Mouse Embryo: A Laboratory Manual (Fourth Edition)]
| [http://cshlpress.com/default.tpl?cart=147944968860031853&action=full&--eqskudatarq=982 Manipulating the Mouse Embryo: A Laboratory Manual (Fourth Edition)]




By Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, & Andras Nagy.
By Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, & Andras Nagy.
|-
| [[File:Reproductive Engineering Techniques.jpg|200px]]
| [http://card.medic.kumamoto-u.ac.jp/card/english/sigen/manual/onlinemanual.html Reproductive Engineering techniques in mice: Technical manual]
| [http://card.medic.kumamoto-u.ac.jp/card/english/sigen/manual/onlinemanual.html Reproductive Engineering techniques in mice: Technical manual]



Revision as of 17:10, 4 August 2017

1. QUIZ

2. Guest Lecturer - Dr Fabien DeleRue

Fabien deleRue profile photo.jpg

Dr Fabien DeleRue

Transgenic Mouse Models of Human Conditions


Dr Delerue is currently in the Transgenic Animal Unit (TAU) his career spans over twenty years practice in animal handling, surgery, and generation of animal models of diseases. His research focuses on the production and characterization of new transgenic mouse models of human genetic disorders, using cutting-edge genome engineering techniques such as CRISPR/Cas9.

Links: UNSW Research Gateway

References

Delerue F & Ittner LM. Genome Editing in Mice Using CRISPR/Cas9: Achievements and Prospects Cloning and Transgenesis 2015, 4:135 doi:10.4172/2168-9849.1000135 [1]

"Animal models are a powerful tool to understand the mechanisms underlying physiological and pathological processes in vivo. To date, mice remain the species most commonly used for genetic manipulation. The recent development of engineered endonucleases such as Zinc Finger Nucleases (ZFN), Transcription activator-like effector nucleases (TALEN), and the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) offered easy, flexible, and fast alternative to ES-Cell based gene targeting. Thanks to multiple advantages, the CRISPR system superseded its predecessors and became a popular method for genome editing. Here, we review the latest techniques to apply CRISPR editing to the mouse genome, and emphasize on the current methods used in transgenic laboratories and subsequent achievements in mice."

Ke YD, van Hummel A, Stevens CH, Gladbach A, Ippati S, Bi M, Lee WS, Krüger S, van der Hoven J, Volkerling A, Bongers A, Halliday G, Haass NK, Kiernan M, Delerue F, Ittner LM.
Short-term suppression of A315T mutant human TDP-43 expression improves functional deficits in a novel inducible transgenic mouse model of FTLD-TDP and ALS. Acta Neuropathologica 2015 Nov;130(5):661-78 PubMed 26437864 [2]

Ittner A, Chua SW, Bertz J, Volkerling A, van der Hoven J, Gladbach A, Przybyla M, Bi M, van Hummel A, Stevens CH, Ippati S, Suh LS, Macmillan A, Sutherland G, Kril JJ, Silva APG, Mackay J, Poljak A, Delerue F, Ke YD, Ittner LM. Site-specific phosphorylation of tau inhibits amyloid-β toxicity in Alzheimer’s mice Science 2016 Nov;354 (6314):904-908 doi: 10.1126/science.aah6205 [3]

Recommended Literature

Manipulating the Mouse Embryo.jpg Reproductive Engineering Techniques.jpg
Manipulating the Mouse Embryo: A Laboratory Manual (Fourth Edition)


By Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, & Andras Nagy.

Reproductive Engineering techniques in mice: Technical manual


Prof Naomi Nakagata, Center for Animal Resources and Development, Kumamoto University, Japan

External Links

External Links Notice - The dynamic nature of the internet may mean that some of these listed links may no longer function. If the link no longer works search the web with the link text or name. Links to any external commercial sites are provided for information purposes only and should never be considered an endorsement. UNSW Embryology is provided as an educational resource with no clinical information or commercial affiliation.

<html5media height="460" width="640">https://www.youtube.com/watch?v=16g6fEup2pQ</html5media>



 2017 ANAT2341 - Timetable | Course Outline | Group Projects | Moodle | Tutorial 1 | Tutorial 2 | Tutorial 3

Labs: 1 Fertility and IVF | 2 ES Cells to Genome Editing | 3 Preimplantation and Early Implantation | 4 Reproductive Technology Revolution | 5 Cardiac and Vascular Development | 6 CRISPR-Cas9 | 7 Somitogenesis and Vertebral Malformation | 8 Organogenesis | 9 Genetic Disorders | 10 Melanocytes | 11 Stem Cells | 12 Group

Lectures: 1 Introduction | 2 Fertilization | 3 Week 1/2 | 4 Week 3 | 5 Ectoderm | 6 Placenta | 7 Mesoderm | 8 Endoderm | 9 Research Technology | 10 Cardiovascular | 11 Respiratory | 12 Neural crest | 13 Head | 14 Musculoskeletal | 15 Limb | 16 Renal | 17 Genital | 18 Endocrine | 19 Sensory | 20 Fetal | 21 Integumentary | 22 Birth | 23 Stem cells | 24 Revision

 Student Projects: 1 Cortex | 2 Kidney | 3 Heart | 4 Eye | 5 Lung | 6 Cerebellum