ANAT2341 Lab 10: Difference between revisions

Latest revision as of 16:43, 30 September 2019

Organogenesis Lab

1. QUIZ

2. Organogenesis Lab

In this lab there will be two main activities:

1. We will open up fertilized eggs with life chicken embryos at around early to late stage gastrulation, ie after 12-24 hours of development. In preparation of this class, please watch this video showing instructions how to open fertile eggs (1 to 2.30 minutes) and some basic experimentation.

2. We will also slice up some flatworms/planarians to witness their capacity to fully regenerate their body parts within two weeks. In preparation of this practical class, please watch the following movie of this amazing little creature featuring Alejandro Sánchez Alvarado, a pioneer in the stem cell field who uses planarians for his research: [1].

Practical Class Notes will be available on Moodle

Additional Chicken Links

Viktor Hamburger (1900 – 2001)

More about chicken embryogenesis: Chicken Development | Hamburger Hamilton Stages

Historic Chicken Embryology
1883 History of the Chick \| 1900 Chicken Embryo Development Plates \| 1904 X-Ray Effects \| 1910 Somites \| 1914 Primordial Germ Cells 1919 Lillie Textbook \| 1920 Chick Early Embryology \| 1933 Neural \| 1939 Sternum \| 1948 Limb \| Movie 1961 \| Historic Papers

stage 1-4
stages 5-10
stages 11-14
stages 15-18
stages 19-21
stages 22-25
stages 26-28
stage 29-32

Additional Planarian Information

Link to Cutting Class Educational Resource

Additional Mouse Development Information

This figure compares the human and mouse developmental stages

More about Mouse embryogenesis: Mouse Timeline Detailed

@@ Line 1: / Line 1: @@
+==Organogenesis Lab==
 == 1. QUIZ ==
+== 2. Organogenesis Lab ==
+In this lab there will be two main activities:
-== 2. Guest Lecturer - Dr. Kimberley Beaumont ==
+. We will open up fertilized eggs with life chicken embryos at around early to late stage gastrulation, ie after 12-24 hours of development. In preparation of this class, please watch [https://www.youtube.com/watch?v=NTUHX1mRfiE this video] showing instructions how to open fertile eggs (1 to 2.30 minutes) and some basic experimentation.
-{|
-| width=185px|[[File:Kim_Beaumont.jpg|180px]]
-Dr. Kimberley Beaumont
-| '''Melanocyte Development, Differentiation and Melanoma'''
-<br>
-Dr Beaumont leads the Melanoma Cell Biology group at the Centenary Institute and is currently focused on investigating the mechanisms underlying melanoma progression and metastasis.
-In her studies she utilizes a 3D melanoma spheroid model, which better mimics the tumour microenvironment compared to conventional 2D cell culture models, as well as mouse models.
+. We will also slice up some flatworms/planarians to witness their capacity to fully regenerate their body parts within two weeks. In preparation of this practical class, please watch the following movie of this amazing little creature featuring Alejandro Sánchez Alvarado, a pioneer in the stem cell field who uses planarians for his research: [https://www.youtube.com/watch?v=Bw1U7_Tk0H0|Movie].
-Dr Beaumont specialises in live, confocal and multi photon microscopy.
+Practical Class Notes will be available on Moodle
-[[Media:Beaumont_devbiol_lecture_2017v2.pdf|Lecture PDF]]
-Links: [http://sydney.edu.au/medicine/people/academics/profiles/kimberley.beaumont.php Sydney University Research Profile] | [https://www.ncbi.nlm.nih.gov/pubmed/?term=beaumont+ka PubMed - Beaumont KA]
-|}
-===References===
-Search PubMed: [https://www.ncbi.nlm.nih.gov/pubmed/?term=beaumont+ka Beaumont KA]
-<pubmed>28634959</pubmed>
-<pubmed>23949769</pubmed>
+===Additional Chicken Links===
+[[File:Viktor Hamburger.jpg|thumb|alt=Viktor Hamburger|link=Embryology History - Viktor Hamburger|Viktor Hamburger (1900 – 2001)]]
+More about chicken embryogenesis: [[Chicken Development]] | [[Hamburger Hamilton Stages]]
+<br>
+{{Chicken links}}
 <br>
-==3. Group Projects==
+<gallery>
+File:HHstage1-4.jpg|stage 1-4
+File:HHstage5-10.jpg|stages 5-10
+File:HHstage11-14.jpg|stages 11-14
+File:HHstage15-18.jpg|stages 15-18
+File:HHstage19-21.jpg|stages 19-21
+File:HHstage22-25.jpg|stages 22-25
+File:HHstage26-28.jpg|stages 26-28
+File:HHstage29-32.jpg|stage 29-32
+</gallery>
-Peer review process should now be completed. I will now unlock the project pages and you can continue editing before submission.
+===Additional Planarian Information===
+[https://cuttingclass.stowers.org/en Link to Cutting Class Educational Resource]
-Now read through your peer reviews of your project.
-# Have many reviews identified specific faults with the current project page?
-# Have many reviews identified specific strengths with the current project page?
-# Are there simple errors that need correction.
-All members of your group have now reviewed all the other project pages.
+===Additional Mouse Development Information===
-# How does your page compare to other projects?
+[[File:Mouse_vs_Human_embryogenesis.jpg]]
-# Have you thought now that parts of your own page that could be improved?
-# Do other groups have good ideas you can incorporate into your own page?
-Remember that peer assessors '''will not''' have identified all issues that will come out in the final assessment.
-# Image information, referencing and copyright will not have been checked in detail. This is a common mistake (look at previous years assessments on their discussion page).
-# Referencing and sources within the project page will not be checked in detail.
-# Parts of your project page may be identified by peer assessment as incomplete, due to a group member not completing their assigned work. You need to contact me so that I can follow up with those individual(s). Remember the whole project mark will be affected by this issue now.
+''This figure compares the human and mouse developmental stages''
+More about Mouse embryogenesis: [[Mouse Timeline Detailed]]
-{{2017ANAT2341 footer}}
+{{2018ANAT2341}}