ANAT2341 Lab 10: Difference between revisions

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==Organogenesis Lab==
== 1. QUIZ ==
== 1. QUIZ ==


== 2. Organogenesis Lab ==
In this lab there will be two main activities:
1. We will open up fertilized eggs with life chicken embryos at around early to late stage gastrulation, ie after 12-24 hours of development. In preparation of this class, please watch [https://www.youtube.com/watch?v=NTUHX1mRfiE this video] showing instructions how to open fertile eggs (1 to 2.30 minutes) and some basic experimentation.
2. We will also slice up some flatworms/planarians to witness their capacity to fully regenerate their body parts within two weeks. In preparation of this practical class, please watch the following movie of this amazing little creature featuring Alejandro Sánchez Alvarado, a pioneer in the stem cell field who uses planarians for his research: [https://www.youtube.com/watch?v=Bw1U7_Tk0H0|Movie].


== 2. Guest Lecturer - Dr. Kimberley Beaumont ==
Practical Class Notes will be available on Moodle
{|
| width=185px|[[File:Kim_Beaumont.jpg|180px]]


Dr. Kimberley Beaumont
===Additional Chicken Links===
| '''Melanocyte Development, Differentiation and Melanoma'''
[[File:Viktor Hamburger.jpg|thumb|alt=Viktor Hamburger|link=Embryology History - Viktor Hamburger|Viktor Hamburger (1900 – 2001)]]
More about chicken embryogenesis: [[Chicken Development]] | [[Hamburger Hamilton Stages]]
<br>
{{Chicken links}}
<br>
<br>
Dr Beaumont leads the Melanoma Cell Biology group at the Centenary Institute and is currently focused on investigating the mechanisms underlying melanoma progression and metastasis.


In her studies she utilizes a 3D melanoma spheroid model, which better mimics the tumour microenvironment compared to conventional 2D cell culture models, as well as mouse models.
<gallery>
File:HHstage1-4.jpg|stage 1-4
File:HHstage5-10.jpg|stages 5-10
File:HHstage11-14.jpg|stages 11-14
File:HHstage15-18.jpg|stages 15-18
File:HHstage19-21.jpg|stages 19-21
File:HHstage22-25.jpg|stages 22-25
File:HHstage26-28.jpg|stages 26-28
File:HHstage29-32.jpg|stage 29-32
</gallery>
 
===Additional Planarian Information===
[https://cuttingclass.stowers.org/en Link to Cutting Class Educational Resource]
 
 


Dr Beaumont specialises in live, confocal and multi photon microscopy.
===Additional Mouse Development Information===
[[File:Mouse_vs_Human_embryogenesis.jpg]]


[[Media:Beaumont_devbiol_lecture_2017v2.pdf]]|Lecture PDF


Links: [http://sydney.edu.au/medicine/people/academics/profiles/kimberley.beaumont.php Sydney University Research Profile] | [https://www.ncbi.nlm.nih.gov/pubmed/?term=beaumont+ka PubMed - Beaumont KA]
''This figure compares the human and mouse developmental stages''
|}


===References===
More about Mouse embryogenesis: [[Mouse Timeline Detailed]]
Search PubMed: [https://www.ncbi.nlm.nih.gov/pubmed/?term=beaumont+ka Beaumont KA]


<pubmed>28634959</pubmed>
<pubmed>23949769</pubmed>


<br>


{{2017ANAT2341 footer}}
{{2018ANAT2341}}

Latest revision as of 17:43, 30 September 2019

Organogenesis Lab

1. QUIZ

2. Organogenesis Lab

In this lab there will be two main activities:

1. We will open up fertilized eggs with life chicken embryos at around early to late stage gastrulation, ie after 12-24 hours of development. In preparation of this class, please watch this video showing instructions how to open fertile eggs (1 to 2.30 minutes) and some basic experimentation.

2. We will also slice up some flatworms/planarians to witness their capacity to fully regenerate their body parts within two weeks. In preparation of this practical class, please watch the following movie of this amazing little creature featuring Alejandro Sánchez Alvarado, a pioneer in the stem cell field who uses planarians for his research: [1].

Practical Class Notes will be available on Moodle

Additional Chicken Links

Viktor Hamburger
Viktor Hamburger (1900 – 2001)

More about chicken embryogenesis: Chicken Development | Hamburger Hamilton Stages

Chicken Links: Introduction | Chicken stages | Hamburger Hamilton Stages | Witschi Stages | Placodes | Category:Chicken
Historic Chicken Embryology  
1883 History of the Chick | 1900 Chicken Embryo Development Plates | 1904 X-Ray Effects | 1910 Somites | 1914 Primordial Germ Cells

1919 Lillie Textbook | 1920 Chick Early Embryology | 1933 Neural | 1939 Sternum | 1948 Limb | Movie 1961 | Historic Papers


Additional Planarian Information

Link to Cutting Class Educational Resource


Additional Mouse Development Information

Mouse vs Human embryogenesis.jpg


This figure compares the human and mouse developmental stages

More about Mouse embryogenesis: Mouse Timeline Detailed


 2018 ANAT2341 - Timetable | Course Outline | Moodle | Tutorial 1 | Tutorial 2 | Tutorial 3

Labs: 1 Preimplantation and Implantation | 2 Reproductive Technology Revolution | 3 Group Projects | 4 GM manipulation mouse embryos | 5 Early chicken eggs | 6 Female reproductive tract | 7 Skin regeneration | 8 Vertebral development | 9 Organogenesis Lab | 10 Cardiac development | 11 Group projects | 12 Stem Cell Journal Club

Lectures: 1 Introduction | 2 Fertilization | 3 Week 1/2 | 4 Week 3 | 5 Ectoderm | 6 Placenta | 7 Mesoderm | 8 Endoderm | 9 Research Technology | 10 Cardiovascular | 11 Respiratory | 12 Neural crest | 13 Head | 14 Musculoskeletal | 15 Limb | 16 Renal | 17 Genital | 18 Endocrine | 19 Sensory | 20 Fetal | 21 Integumentary | 22 Birth | 23 Stem cells | 24 Revision

 Student Projects: Group Projects Information Project 1 | Project 3 | Project 4 | Project 5 | 2018 Test Student | Copyright