ANAT2341 Lab 10: Difference between revisions

Latest revision as of 16:43, 30 September 2019

Organogenesis Lab

1. QUIZ

2. Organogenesis Lab

In this lab there will be two main activities:

1. We will open up fertilized eggs with life chicken embryos at around early to late stage gastrulation, ie after 12-24 hours of development. In preparation of this class, please watch this video showing instructions how to open fertile eggs (1 to 2.30 minutes) and some basic experimentation.

2. We will also slice up some flatworms/planarians to witness their capacity to fully regenerate their body parts within two weeks. In preparation of this practical class, please watch the following movie of this amazing little creature featuring Alejandro Sánchez Alvarado, a pioneer in the stem cell field who uses planarians for his research: [1].

Practical Class Notes will be available on Moodle

Additional Chicken Links

Viktor Hamburger (1900 – 2001)

More about chicken embryogenesis: Chicken Development | Hamburger Hamilton Stages

Historic Chicken Embryology
1883 History of the Chick \| 1900 Chicken Embryo Development Plates \| 1904 X-Ray Effects \| 1910 Somites \| 1914 Primordial Germ Cells 1919 Lillie Textbook \| 1920 Chick Early Embryology \| 1933 Neural \| 1939 Sternum \| 1948 Limb \| Movie 1961 \| Historic Papers

stage 1-4
stages 5-10
stages 11-14
stages 15-18
stages 19-21
stages 22-25
stages 26-28
stage 29-32

Additional Planarian Information

Link to Cutting Class Educational Resource

Additional Mouse Development Information

This figure compares the human and mouse developmental stages

More about Mouse embryogenesis: Mouse Timeline Detailed

@@ Line 1: / Line 1: @@
-==Skin Development and Regeneration==
+==Organogenesis Lab==
+== 1. QUIZ ==
+== 2. Organogenesis Lab ==
+In this lab there will be two main activities:
+. We will open up fertilized eggs with life chicken embryos at around early to late stage gastrulation, ie after 12-24 hours of development. In preparation of this class, please watch [https://www.youtube.com/watch?v=NTUHX1mRfiE this video] showing instructions how to open fertile eggs (1 to 2.30 minutes) and some basic experimentation.
+. We will also slice up some flatworms/planarians to witness their capacity to fully regenerate their body parts within two weeks. In preparation of this practical class, please watch the following movie of this amazing little creature featuring Alejandro Sánchez Alvarado, a pioneer in the stem cell field who uses planarians for his research: [https://www.youtube.com/watch?v=Bw1U7_Tk0H0|Movie].
+Practical Class Notes will be available on Moodle
+===Additional Chicken Links===
+[[File:Viktor Hamburger.jpg|thumb|alt=Viktor Hamburger|link=Embryology History - Viktor Hamburger|Viktor Hamburger (1900 – 2001)]]
+More about chicken embryogenesis: [[Chicken Development]] | [[Hamburger Hamilton Stages]]
+<br>
+{{Chicken links}}
+<br>
+<gallery>
+File:HHstage1-4.jpg|stage 1-4
+File:HHstage5-10.jpg|stages 5-10
+File:HHstage11-14.jpg|stages 11-14
+File:HHstage15-18.jpg|stages 15-18
+File:HHstage19-21.jpg|stages 19-21
+File:HHstage22-25.jpg|stages 22-25
+File:HHstage26-28.jpg|stages 26-28
+File:HHstage29-32.jpg|stage 29-32
+</gallery>
+===Additional Planarian Information===
+[https://cuttingclass.stowers.org/en Link to Cutting Class Educational Resource]
-== 1. QUIZ ==
-== 2. Dr. Annemiek Beverdam (UNSW) - "Stem cell regulation in normal skin regeneration and in skin disease" ==
+===Additional Mouse Development Information===
+[[File:Mouse_vs_Human_embryogenesis.jpg]]
-[[File:Annemiek Beverdam profile photo.jpeg]]
-Dr. Annemiek Beverdam studies the genetic processes that govern development, homeostasis and regeneration of the skin in the mouse. Her research aims at understanding the genetic and molecular basis of developmental and human regenerative skin diseases such as skin cancer, which affects 2 out of 3 Australians in their life time. Her lab recently made the pivotal discoveries that Yes-associated protein (YAP) functions acts as a key molecular switch in epidermal stem/progenitor cell proliferation and differentiation [https://www.ncbi.nlm.nih.gov/pubmed/23190885] by driving β-Catenin Activation [https://www.ncbi.nlm.nih.gov/pubmed/27816394]. Dr. Beverdam currently investigates the developmental genetic context in which YAP functions to control skin stem/progenitor cells in normal and in disrupted skin biology. She employs genetically manipulated mouse models, human skin samples, advanced imaging technology such as confocal microscopy and whole mouse in vivo imaging, gene and protein expression analyses and whole genome approaches to address her research questions. Her research will open up exciting new avenues for translational research and the development of treatments for human regenerative skin disease such as skin cancer and eczema.
+''This figure compares the human and mouse developmental stages''
-'''References:'''
+More about Mouse embryogenesis: [[Mouse Timeline Detailed]]
-Akladios B., Mendoza-Reinoso V., Samuel MS.,  Hardeman E., Khosrotehrani K., Key B; Beverdam A (2016). ‘Epidermal YAP2-5SA-ΔC Drives β-Catenin Activation to Promote Keratinocyte Proliferation in the Mouse Skin in vivo’ [https://www.ncbi.nlm.nih.gov/pubmed/27816394].
-Liang H*, Akladios B*, Canales CP*, Francis R, Hardeman EH, Beverdam A. (2016) CUBIC protocol visualizes protein expression at single cell resolution in whole mount skin preparations (2016). JoVE [https://www.ncbi.nlm.nih.gov/pubmed/27584943].
-Corley SM, Canales C, Carmona-Mora P, Mendoza-Reinoso V, Beverdam A, Hardeman EH, Wilkins M, Palmer S. (2016). RNA-Seq analysis of Gtf2ird1 knockout epidermal tissue provides potential insights into molecular mechanisms underpinning Williams-Beuren syndrome. BMC Genomics [https://www.ncbi.nlm.nih.gov/pubmed/27295951].
-Beverdam A., Claxton C., Zhang., X., James G., Harvey KF, Key B. (2013). YAP controls stem/progenitor cell proliferation in the mouse postnatal epidermis. Journal of Investigative Dermatology [https://www.ncbi.nlm.nih.gov/pubmed/23190885]
+{{2018ANAT2341}}