ANAT2241 Virtual Slides - Example Spot Images

From Embryology
Revision as of 16:05, 22 March 2018 by Z8600021 (talk | contribs)

ANAT2241 course banner.jpg

ANAT2241 This practical support page content is not part of the virtual science practical class and provides additional information for student self-directed learning purposes. All practical class pages are located on Moodle - 2019 ANAT2241

The Spot Test

  • 20 questions.
  • 2 minutes / question. Clock
  • will be Powerpoint slides generated from the virtual slide images used in the practical classes.
  • cover up to and including PNS/CNS practical classes.
  • may have more than one part to each question.
  • may include electron micrograph images.



Example Images below only - Not indicative of final Spot Test presentation.

  1. Specifically identify this epithelium
  2. Specifically identify this cell
  3. 1. Specifically identify the cells in this region and 2. The cell process occurring here
  4. Specifically identify this structure
  5. Specifically identify this cell type
  6. Identify this muscle tissue and location

Course Links

Moodle - ANAT2241 - 2019

Histology Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | ANAT2241 Support | Histology | Histology Stains | Embryology Glossary


Common Histology Stains  
Histology Stains - Common Stains and Their Reactions
Stain
Nucleus
Cytoplasm
Collagen
RBCs
Other
Haematoxylin
blue
-
-
-
mucins - light blue
Eosin
-
pink
pale pink
bright red
colloid - pinkmuscle - red
Iron Haematoxylin
blue/black
-
-
-
Van Gieson
-
brown/yellow
red
yellow
muscle: yellow/browncartilage - pink
Verhoeff's Elastin
black
-
-
-
elastic fibres - black
Tartrazine
-
yellow
yellow
yellow
Silver Impregnation
-
-
grey/brown
-
reticular fibres - black
Methyl Green
dark green
light green
light green
green
Nuclear Fast Red
red
pink
pink
pink
Gomori's Trichrome
purple/red
purple
green
red
keratin - redmuscle - purple/red
Heidenhain's Azan
red
purple/red
deep blue
red
muscle - red
Osmium Tetroxide
-
-
brown
brown
myelin, lipids - black
Alcian Blue
-
-
-
-
mucins, - blue
Periodic acid-Schiff (PAS)
-
-
pink
-
mucins, glycogen, glycocalyx - magenta
Phosphotungstic Acid-Hematoxylin (PTAH)
blue
-
red
blue
muscle bands - blue
Masson's Trichrome
blue/black
red
green/blue
red
cartilage, mucins - blue or green; muscle - red
Luxol Fast Blue
-
-
-
variable
myelin - blue
Aldehyde Fuchsin
-
-
-
-
elastic fibres, mast cells - deep purple
Light Green
-
-
light green
-
Gallocyanin
dark blue
-
-
-
nucleic acids, Nissl granules - dark blue
Romanowsky (e.g. Leishman's)
blue
pink
acidophils - red, basophils - blue, azurophilic - purple
Aldehyde Pararosanilin elastic fibres - purple
Haematoxylin and Eosin
One of the most common staining techniques in pathology and histology. Acronym "H and E" stain. (H&E, HE).


Haematoxylin
  • Stains nuclei blue to dark-blue.
  • Stains the matrix of hyaline cartilage, myxomatous, and mucoid material pale blue.
  • Stains myelin weakly but is not noticeable if combined with eosin stain.
  • combined with Orange G (H & Or. G.) instead of eosin, specifically stains the granules of acidophilic cells of the adenohypophysis (anterior pituitary).
Eosin
  • Stains cytoplasm pink to red; red blood cells are also bright red.
  • Common counterstain to hematoxylin.
  • Stain intensity varies with the formula as well as the fixative.

Practical Support

Pages can be accessed from any internet connected computer.

ANAT2241 Support Links: The Virtual Microscope | Covering and Lining Epithelia | Glandular Epithelia | CT Components | CT Types | Bone, Bone Formation and Joints | Muscle | Nervous | Blood | Eye | Cardiovascular | Respiratory | Integumentary | Gastrointestinal | Gastrointestinal Organs | Lymphatic and Immune | Endocrine | Urinary | Female Reproductive | Male Reproductive | Histology Stains | Histology Drawings | Practicals Health and Safety 2013 | Moodle - 2019


ANAT2241 This practical support page content is not part of the science practical class and provides only background information for student self-directed learning purposes.


Cite this page: Hill, M.A. (2019, December 13) Embryology ANAT2241 Virtual Slides - Example Spot Images. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/ANAT2241_Virtual_Slides_-_Example_Spot_Images

What Links Here?
© Dr Mark Hill 2019, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G