ANAT2241 Covering and Lining Epithelia

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ANAT2241 This practical support page content is not part of the virtual science practical class and provides additional information for student self-directed learning purposes. All practical class pages are located on Moodle - ANAT2241

General Objective

Histology terminology cartoon.jpg

To learn the structure and function of covering and lining epithelia.


Associated lecture slides: Covering and Lining Epithelia (17.51 MB PDF) | 4 slides/page

Specific Objectives

  1. To identify covering and lining epithelia.
  2. To understand the terms endothelium, and mesothelium.
  3. To classify epithelia on the basis of cell morphology and cell arrangement (e.g. layering), noting the general absence of blood vessels in epithelia.
  4. To study the surface specializations of some epithelial cells: brush or striated border (microvilli), non-motile stereocilia and motile cilia and to recognise these specialisation in electron micrographs.

Learning Activities

To identify, draw and label the various types of epithelium (simple, stratified and pseudostratified) and to discuss some of the special functional features associated with their structure.


Virtual Slides: Covering and Lining Epithelia


Epithelium histology cartoon 01.jpg Epithelium histology cartoon 02.jpg
Epithelia cell shape Epithelia sectioning appearance

Histology

Epithelium forms continuous layers of cells that cover surfaces and line cavities of the body.

Simple epithelium

Vein histology 01.jpg

Venule with endothelium, a simple squamous epithelium lining.

Thyroid histology 002.jpg

Thyroid follicles, with a simple cuboidal epithelium surrounding each follicle.

Pseudostratified epithelium

Trachea histology 01.jpg

Trachea lined with a pseudostratified epithelium.

Testis histology 015.jpg

Testis ductus epididymidis lined with a pseudostratified epithelium.

Testis histology 023.jpg

Testis ductus epididymidis lined with a pseudostratified epithelium.

Stratified epithelium

Oesophagus stratified epithelium

Transitional epithelium

Bladder histology 01.jpg

Urinary bladder transitional epithelium

Ureter histology 001.jpg

Ureter transitional epithelium

Unlabeled Images

Course Links

Moodle - ANAT2241 - 2019

Histology Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | ANAT2241 Support | Histology | Histology Stains | Embryology Glossary


Common Histology Stains  
Histology Stains - Common Stains and Their Reactions
Stain
Nucleus
Cytoplasm
Collagen
RBCs
Other
Haematoxylin
blue
-
-
-
mucins - light blue
Eosin
-
pink
pale pink
bright red
colloid - pinkmuscle - red
Iron Haematoxylin
blue/black
-
-
-
Van Gieson
-
brown/yellow
red
yellow
muscle: yellow/browncartilage - pink
Verhoeff's Elastin
black
-
-
-
elastic fibres - black
Tartrazine
-
yellow
yellow
yellow
Silver Impregnation
-
-
grey/brown
-
reticular fibres - black
Methyl Green
dark green
light green
light green
green
Nuclear Fast Red
red
pink
pink
pink
Gomori's Trichrome
purple/red
purple
green
red
keratin - redmuscle - purple/red
Heidenhain's Azan
red
purple/red
deep blue
red
muscle - red
Osmium Tetroxide
-
-
brown
brown
myelin, lipids - black
Alcian Blue
-
-
-
-
mucins, - blue
Periodic acid-Schiff (PAS)
-
-
pink
-
mucins, glycogen, glycocalyx - magenta
Phosphotungstic Acid-Hematoxylin (PTAH)
blue
-
red
blue
muscle bands - blue
Masson's Trichrome
blue/black
red
green/blue
red
cartilage, mucins - blue or green; muscle - red
Luxol Fast Blue
-
-
-
variable
myelin - blue
Aldehyde Fuchsin
-
-
-
-
elastic fibres, mast cells - deep purple
Light Green
-
-
light green
-
Gallocyanin
dark blue
-
-
-
nucleic acids, Nissl granules - dark blue
Romanowsky (e.g. Leishman's)
blue
pink
acidophils - red, basophils - blue, azurophilic - purple
Aldehyde Pararosanilin elastic fibres - purple
Haematoxylin and Eosin
One of the most common staining techniques in pathology and histology. Acronym "H and E" stain. (H&E, HE).


Haematoxylin
  • Stains nuclei blue to dark-blue.
  • Stains the matrix of hyaline cartilage, myxomatous, and mucoid material pale blue.
  • Stains myelin weakly but is not noticeable if combined with eosin stain.
  • combined with Orange G (H & Or. G.) instead of eosin, specifically stains the granules of acidophilic cells of the adenohypophysis (anterior pituitary).
Eosin
  • Stains cytoplasm pink to red; red blood cells are also bright red.
  • Common counterstain to hematoxylin.
  • Stain intensity varies with the formula as well as the fixative.

Practical Support

Pages can be accessed from any internet connected computer.

ANAT2241 Support Links: The Virtual Microscope | Covering and Lining Epithelia | Glandular Epithelia | CT Components | CT Types | Bone, Bone Formation and Joints | Muscle | Nervous | Blood | Eye | Cardiovascular | Respiratory | Integumentary | Gastrointestinal | Gastrointestinal Organs | Lymphatic and Immune | Endocrine | Urinary | Female Reproductive | Male Reproductive | Histology Stains | Histology Drawings | Practicals Health and Safety 2013 | Moodle - 2019


ANAT2241 This practical support page content is not part of the science practical class and provides only background information for student self-directed learning purposes.


Cite this page: Hill, M.A. (2024, March 28) Embryology ANAT2241 Covering and Lining Epithelia. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/ANAT2241_Covering_and_Lining_Epithelia

What Links Here?
© Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G