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ANAT2241 This practical support page content is not part of the virtual science practical class and provides additional information for student self-directed learning purposes. All practical class pages are located on Moodle - ANAT2241
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Aims
Currently a template page - this notice removed when complete.
Histology
Course Links
Moodle - ANAT2241 - 2019
- Histology Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | ANAT2241 Support | Histology | Histology Stains | Embryology Glossary
Common Histology Stains
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Histology Stains - Common Stains and Their Reactions
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Stain
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Nucleus
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Cytoplasm
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Collagen
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RBCs
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Other
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Haematoxylin
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blue
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-
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-
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-
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mucins - light blue
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Eosin
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-
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pink
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pale pink
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bright red
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colloid - pinkmuscle - red
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Iron Haematoxylin
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blue/black
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-
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-
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-
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Van Gieson
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-
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brown/yellow
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red
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yellow
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muscle: yellow/browncartilage - pink
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Verhoeff's Elastin
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black
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-
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-
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-
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elastic fibres - black
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Tartrazine
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-
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yellow
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yellow
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yellow
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Silver Impregnation
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-
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-
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grey/brown
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-
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reticular fibres - black
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Methyl Green
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dark green
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light green
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light green
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green
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Nuclear Fast Red
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red
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pink
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pink
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pink
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Gomori's Trichrome
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purple/red
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purple
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green
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red
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keratin - redmuscle - purple/red
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Heidenhain's Azan
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red
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purple/red
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deep blue
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red
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muscle - red
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Osmium Tetroxide
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-
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-
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brown
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brown
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myelin, lipids - black
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Alcian Blue
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-
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-
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-
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-
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mucins, - blue
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Periodic acid-Schiff (PAS)
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-
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-
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pink
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-
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mucins, glycogen, glycocalyx - magenta
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Phosphotungstic Acid-Hematoxylin (PTAH)
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blue
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-
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red
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blue
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muscle bands - blue
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Masson's Trichrome
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blue/black
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red
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green/blue
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red
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cartilage, mucins - blue or green; muscle - red
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Luxol Fast Blue
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-
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-
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-
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variable
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myelin - blue
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Aldehyde Fuchsin
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-
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-
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-
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-
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elastic fibres, mast cells - deep purple
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Light Green
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-
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-
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light green
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-
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Gallocyanin
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dark blue
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-
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-
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-
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nucleic acids, Nissl granules - dark blue
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Romanowsky (e.g. Leishman's)
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blue
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pink
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acidophils - red, basophils - blue, azurophilic - purple
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Aldehyde Pararosanilin
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elastic fibres - purple
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Haematoxylin and Eosin
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One of the most common staining techniques in pathology and histology. Acronym "H and E" stain. (H&E, HE).
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Haematoxylin
- Stains nuclei blue to dark-blue.
- Stains the matrix of hyaline cartilage, myxomatous, and mucoid material pale blue.
- Stains myelin weakly but is not noticeable if combined with eosin stain.
- combined with Orange G (H & Or. G.) instead of eosin, specifically stains the granules of acidophilic cells of the adenohypophysis (anterior pituitary).
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Eosin
- Stains cytoplasm pink to red; red blood cells are also bright red.
- Common counterstain to hematoxylin.
- Stain intensity varies with the formula as well as the fixative.
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Practical Support
- Pages can be accessed from any internet connected computer.
ANAT2241 Support Links: The Virtual Microscope | Covering and Lining Epithelia | Glandular Epithelia | CT Components | CT Types | Bone, Bone Formation and Joints | Muscle | Nervous | Blood | Eye | Cardiovascular | Respiratory | Integumentary | Gastrointestinal | Gastrointestinal Organs | Lymphatic and Immune | Endocrine | Urinary | Female Reproductive | Male Reproductive | Histology Stains | Histology Drawings | Practicals Health and Safety 2013 | Moodle - 2019
ANAT2241 This practical support page content is not part of the science practical class and provides only background information for student self-directed learning purposes.
Cite this page: Hill, M.A. (2024, April 24) Embryology ANAT2241 Connective Tissue Types. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/ANAT2241_Connective_Tissue_Types
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- © Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G