Difference between revisions of "2017 Group Project 4"

From Embryology
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|'''Lens pit'''||A pit-like depression in the fetal head where the lens develops.  
|'''Lens pit'''||A pit-like depression in the fetal head where the lens develops.  
|'''Lens placode'''||The placode developing in the ectoderm overlying the optic vesicle, eventually becoming the lens of the eye.
|'''Lens vesicle'''||The ectodermal invagination in the embryo formed opposite the optic cup and gives rise to the lens.
|'''Lumina'''||the central cavity of a tubular structure.
|'''Lumina'''||the central cavity of a tubular structure.
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|'''Optic cup'''||A structure of the diencephalon formed after optic vesicle folding. This structure gives rise to the retina.  
|'''Optic cup'''||A structure of the diencephalon formed after optic vesicle folding. This structure gives rise to the retina.  
|'''Optic groove'''||Two small grooves on each side of the developing forebrain in the neural folds. Also chiasmatic groove.
|'''Optic nerve'''||Cranial nerve II which transmits impulses to the brain from visual information detected by the photoreceptive retina.
|'''Optic nerve'''||Cranial nerve II which transmits impulses to the brain from visual information detected by the photoreceptive retina.
|'''Optic stalk'''||Pair of slender embryonic structures that give rise to the optic nerve  
|'''Optic stalk'''||Pair of slender embryonic structures that give rise to the optic nerve.
|'''Optic vesicle'''||An evagination of each lateral all of the embryonic vertebrate forebrain from which the nervous structures of the eye develop.  
|'''Optic vesicle'''||An evagination of each lateral all of the embryonic vertebrate forebrain from which the nervous structures of the eye develop.  

Revision as of 09:06, 26 October 2017

2017 Student Projects 
Student Projects: 1 Cerebral Cortex | 2 Kidney | 3 Heart | 4 Eye | 5 Lung | 6 Cerebellum
Student Page - here is the sample page I demonstrated with in the first labs.I remind all students that you have your own Group Forum on Moodle for your discussions, it is only accessible by members of your group.
Editing Links: Editing Basics | Images | Tables | Referencing | Journal Searches | Copyright | Font Colours | Virtual Slide Permalink | My Preferences | One Page Wiki Card | Printing | Movies | Language Translation | Student Movies | Using OpenOffice | Internet Browsers | Moodle | Navigation/Contribution | Term Link | Short URLs | 2018 Test Student

Eye Development


Anatomy of the Adult Eye

The eye is a complex sensory structure which allows a variety of species to intake and process visual information from the world around us.

To discuss the key anatomical components of the eye, we will break down the eye in parts[1]:

Supporting Structures (Orbit, Extraoccular Muscles, Lacrimal Gland, Eyelid)

Figure 1. Layers of the Eyeball

The orbit of the eye consists of a framework of bones and connective tissue which provide structural support and protection to the sensitive human eye. 7 bones contribute to the orbit of the eye: frontal, lacrimal, sphenoid, zygomtatic, ethmoid, maxilary, palatine. Several openings exist in the orbital structure, 2 key fissures are the superior orbital fissure and inferior orbital fissure and the posterior optic canal. These openings allow crainial nerves passageway.

A set of 6 extraoccular muscles allow for a strong voluntary control of the movement of the eye. These muscles include superior recuts, inferior rectus, lateral rectus, medial rectus, inferior oblique, superior oblique muscles. These muscles are some of the smallest in the human body and are designed to produce fast, controlled motion to focus on an object of interest.

The eyelid is the anterior covering of the eye, consisting of a thin fold of skin. It is controlled by the levitator palpabrae superioris muscle. Superio-laterally to the eyelid is the lacrimal gland, which serves to create a film of tear, an important component to keeping the anterior surface of the eye moist. The eyelid assists in this process by opening and closing, or blinking, which spreads the tear film across the surface of the eye. Tear film and debris caught in the tear film are excreted through the lacrimal duct which is located on the infero-medial surface of the eyelid.

Figure 2. Anterior Part of the Eye

Layers of the Eyeball (Sclera, Choroid, Retina)

The superficial layers of the eyeball is the sclera, a thick fibrous layer which becomes thicker as it extends posteriorly. The sclera is the visible as the white part of our eye. It's function is to provide structural stability to the eye and serves as attachment for some extraoccular insertions. Deep to the sclera is the choroid, a vascular network which provides nourishment to the layers of the eyeball and plays a large role in the growth of the eye. Deep to the choroid is the retina, a network of nerve fibers which transmit visual information. The retina contains 2 types of photoreceptors which convert visible light into nerve impulses. Rods work best in low light environments while cones work best in high light enthronements and detect colour and sharp detail.[2]: The retina does not extend to the anterior part of the eyeball, unlike the sclera and choroid layers.

Anterior structure (Lens, Cornea, Pupil, Iris, Ciliary Body, Conjunctiva Anterior Chamber)

The most anterior component of the eyeball is the cornea, which is a multilayered avascular transparent structure who's major function to the refract light to assist image formation (contributes about 75% of total refraction). Continuous with cornea superiorly and inferiorly is the conjunctiva. Deep to the cornera is the iris, or the coloured ring visible in the eye. The iris consists of 2 muscles which act to enhance and diminish the size of the pupil, a hole in the middle of the iris allowing light to enter the eye. Deep to the pupil and iris is the lens, which is a transparent structure which contributes the other 25% of the eye's refractive power. The lens changes shape through accomodation controlled by the ciliary body muscles. The ciliary body is the anterior extension of the choroid. The function of the lens is to focus images by changing refractive power based on the position and depth of focal objects. In between the anterior surface of the lens and posterior surface of the cornea is the anterior chamber. This chamber is filled with aqueous humor supplied by the ciliary body, who's function is to provide nutrients to ocular structures and maintain intraocular pressure.

Figure 3. Posterior Part of the Eye

Posterior structure (Optic Nerve, Optic Disc, Macula, Fovea, Posterior Chamber)

In the posterior section of the retina is the macula, a central spot in which a high concentration of cone photoreceptors are found. The direct centre of the macula is called the fovea which contains solely cone photoreceptors. The fovea is only part of the eye which can conduct 'perfect' 20/20 vision. It has a high workload and hence a strong vascular supporting structure[3]:. The most posterior part of the eye is the optic nerve (along with the central retinal artery and central retinal vein), which is responsible for transporting nerve impulses to the cerebral cortex for processing. The opening of the optic nerve is called the optic disc, which contains no photoreceptors and is a blind spot of the eye. Between the posterior surface of the lens, the retina and the optic disc is the posterior chamber. The posterior chamber contains a vitreous humour, a jelly like substance with a high viscosity.

Embryonic Contributions

Eye development is a complex process. It involves the formation of the retina and the lens, which we together call the eyeball. It also involves the formation of auxiliary eye structures which are the eyelid, lacrimal gland, and cornea. The retina is formed from the neuroectoderm, the lens from the surface ectoderm and the auxiliary tissues are formed from the head surface ectoderm, neural crest cells and the head mesoderm [4].

Figure 4. Stage 14
Embryonic contributions Eye component
Neuroectoderm of the forebrain Retina, Posterior layers of the iris, The optic nerve
Surface ectoderm of the head The lens of the eye, The corneal epithelium
Mesoderm between the neuroectoderm and the surface ectoderm The fibrous and vascular coats of the eye
Neural crest cells Choroid, Sclera, Corneal endothelium

Timeline of embryonic development

3-4 Eye fields, Optic vesicles
5-6 Optic Cup, Lens Vesicle, Choroid Fissure, Hyaloid Artery
7-8 Cornea, Anterior Chamber, Pupillary Membrane, Lens, Retina
8-10 Eyelids
9-15 Iris, Ciliary Body

Carnegie Stages

Carnegie Stages is a system of 23 stages used to describe developmental events of the vertebrate embryo. At stage 10 we see early signs related to eye development. The table below is an made of knowledge from Anthony A. Person's article [5].

Stage Events
Stage 10 (22 days) The optic primordium has developed. Two small grooves develop on each side of the developing forebrain in the neural folds - this is the optic grooves. The optic placode has begun to develop, which is seen by a small thickening of the surface ectoderm lateral to the hindbrain.
Stage 11 (24 days) Optic vesicle begins to form from the optic groove.

Figure 1. Stage 11

Stage 12 (26 days) The optic vesicles have extended from the forbrain to the surface ectoderm. The optic vesicle now lies close to the surface ectoderm.
Stage 13 (28 days) The optic vesicle interacts with the surface ectoderm and will induce this ectoderm to form the lens placode, which is the precursor of the lens. Optic evagination differentiation makes it possible to identiy optic parts of retina, future pigmented layer of retina and optic stalk.

Figure 2. Stage 13

Stage 14 (32 days) The lens placode is indented by the lens pit and is be cup-shaped.
Stage 15 (33 days) The lens pit will close and form a circle that separates from the surface ectoderm and becomes the lens vesicle. The lens vesicle and optic cub lie close to the surface ectoderm, which creates a slight elevation in the region of the eye.
Stage 16 (37 days ) The lens body has grown and now has a D-shaped lens cavity. First indication of the development of the eyelids visible as eyelid grooves above and below the eye.
Stage 17 - 19 Retinal pigment is visible and the retinal fissure is almost closed. The eyes are still laterally placed but starts to take a more anterior position. The lower eyelid fold develops first and then the eyelid fold above the eye.
Stage 18 (44 days) Mesenchyme invades the region between the lens epithelium and the surface ectoderm. The eyes have shifted to a more anterior position. The groove above and below the eyes are deeper but have not joined yet.
Stage 19 - 22 The eyelid folds develop into the eyelids and the upper and the lower eyelids now meet at the outer canthus, which is the corner of the eye.

Figure 3. Stage 22

Stage 20 (51 days) The lens cavity is lost. The upper and lower lids meet laterally and medially. The eyelids now partly cover the eye. Lens suture begins to form.
Stage 23 (57 days) End of embryonic period. The face begins to look human. Eyelids closure is complete.

Development of the eye components


Figure 5. Stages of lens formation in mouse embryos

The eye starts to develop at 22 days. The optic grooves (sulci) appears in the neural folds at the cranial end of the embryo. When the neural fold fuse to form the forebrain, the optic grooves will form optic vesicles. The optic vesicles are continuous cavities from the cavity of the forebrain and project from the wall of the forebrain and into the mesenchyme. The optic vesicle extends from the diencephalon and will come in contact with the surface ectoderm of the head. This induces the formation of a lens placode.

The surface ectoderm near the optic vesicles will thicken and form the lens placodes. The lens placodes will sink into the surface ectoderm and form lens pits. The edges of the lens pits will travel towards each other and fuse to form round lens vesicles, which will later lose connection with the surface ectoderm. The optic vesicles do also keep developing - they will form double-walled optic cups which are connected to the brain by the optic stalk. The two layers of the optic cup will differentiate in different directions. The cells of the outer layer will produce melanin pigment and later become the pigmented retina. The cells of the inner layer of the optic cup will proliferate fast and develop glia, ganglion cells, interneurons and light-sensitive photoreceptor neurons. These cells are in the neural retina. The ganglion cells of the retina are neurons that send signals to the brain. The axons of the ganglion cells of the neural retina will grow in the wall of the optic stalk. The cavity in the optic nerve will start disappearing, and instead, the axons of the ganglion cells will form the optic nerve. The optic stalk is now the optic nerve [6]

The optic cups will fold inwards around the lens while the lens vesicles have grown inwards so they have fully lost their connection with the surface ectoderm, which locates them in the cavities of the optic cups. The retinal fissures (linear grooves) will develop and cover the ventral surface of the optic cups and down to the optic stalk. The retinal fissures contain vascular mesenchyme and hyaloid blood vessels will develop here. The hyaloid artery supplies the structures in the eye with blood and the hyaloid vein will return the blood from these structures [6].

Formation of the optic vesicle

It is a specific area of the neural ectoderm that will become the optic vesicle - this happens because of a group of transcription factors - Six3, Pax6, and Rx1. These transcription factors are expressed in the most anterior tip of the neural plate. This area will split into bilateral regions and form the optic vesicles. The Pax6 protein has shown to be especially important for the development of the lens and retina. This protein is important for photoreceptive cells in all phyla. Pax 6 is also present in the murine forebrain, hindbrain, and nasal placodes, but the eyes are most sensitive its absence [6].

The sonic hedgehog gene is important for the separation of the single eye field into two fields. If this gene is inhibited, the eye field will not split which will result in cyclopia, a single eye in the center of the face [6].


Figure 6. Diagram of developing lens and optic cup

The retina is the essential component of the eye with the primary function of photoreception [7]. Formation of the retina begins with the specification of retinal cells in the anterior neuroectoderm. First morphological sign of eye development is the formation of two lateral grooves in the anterior neuroectoderm, which is called the optic sulci. The cells of the optic sulci will evaginate and form the optic vesicle. The distal portion of the optic vesicle will form the retina and the proximal will form the optic stalk [8]. The retina develops from the walls of the optic cup, these walls develop into the two layers of the retina. The thin, outer layer of the optic cup will become the pigment layer of the retina and the thick, inner layer of the optic cup will differentiate into the neural retina. Forkhead transcription factors are regulating the proliferation and differentiation of the retinal precursor cells. During the embryonic and early fetal periods, the two layers of the retina are separated which makes an intraretinal space. The intraretinal space is derived from the cavity of the optic cup. The two layers of the retina will fuse and the intraretinal space will gradually disappear [9].

When the lens is developing the inner layer of the optic cup starts to proliferate and forms a thick neuroepithelium which will later differentiate into the neural retina. The neural retina contains photoreceptors and the cell bodies of neurons and is the light-sensitive region of the retina. The axons of the ganglion cells in the neural retina grow in the wall of the optic stalk and will form the optic nerve [9].


Human lens induction occurs at around 28 days and is completed around day 56. The surface ectoderm will thicken near the optic vesicle and create the lens placode and later form the lens vesicle [10]. Lens cells come from ectoderm and differentiate into either lens fibers or the lens epithelium. The anterior monolayer of epithelial cells of the lens will create the lens epithelium, which makes the sheet of cuboidal epithelium covering the anterior surface of the lens. The posterior lens vesicle cells will produce the linear primary fibre cells aligned parallel to the optic axis. These fibres will create the lens mass and form the embryonic lens nucleus. The lens epithelial cells will keep proliferating and produce new cells which generate a secondary lens fiber cells. This rows of cell will form the outer shells and keep the lens growing throughout life. This makes the eye lens unique - it will have an addition of new cells inside the surrounding capsule all the time [11].

Ciliary Body

The ciliary body is a muscular and secretory tissue and is located directly behind the lens. The ciliary body forms part of the anterior segment of the eye and is an important regulator of eye physiology and the vision. The ciliary body produces the aqueous fluid which fills the eyes and nourishes the lens and cornea - this aqueous fluid function to keep the eye in a pressurized and inflated state, which is important the vision.The ciliary body also synthesizes collagenIX and tenasin-C [12].

The ciliary body extends from the iris root (anteriorly) to the ora serrata (posteriorly). It consist of ciliary muscles and ciliary processes. Each ciliary process (fold) is covered by a double-layered secretory epithelium; the outer pigmented and the inner unpigmented (closes to the lens). The epithelial layers of the ciliary body comes from the retina of the optic cup. We see two different ciliary body epithelium: the inner non-pigmented ciliary epithelium which is connected with the neural retina and the outer pigmented ciliary epithelium which is connected with the retinal pigmented epithelium. The epithelium of the iris is the further anterior extension. The epithelial layers are associated with a stroma containing the ciliary muscle. The ciliary muscle is complex, but can be divided into three portions; anterior, posterior and internal [13].

In the chick eye development, we see the mesenchyme grow together on the margin of the optic cup and will form the stroma of the ciliary body and the iris, which is located more anteriorly. It is unknown how if the lens has a role in inducing the secretory ciliary body epithelium and the muscular iris epithelium [14].


The iris is the circular, pigmented muscular portion of the eye with a central aperture that is the pupil. The main function of this round muscle is to manipulate pupil diameter as an adjustment to various conditions and to control the amount of light entering the eye [15]. This is influenced by dilator pupillae (radial) and sphincter pupillae (constricting) muscles. Iris pigmentation is dependent on the abundance and distribution of certain types of melanin in the posterior epithelium of the iris (eumelanin and pheomelanin) [15].

The Iris develops at the end of the third month of development. The iris is a thin layer and derives from the anterior rim of the optic cup [16]. The iris is formed by the pigment-containing internal and external layers of the optic cup and by a layer of vascularised connective tissue which also contains the pupillary muscles. Internal layers of the optic cup form the non-pigmented iris parts whereas the external layers of the optic cup develop into the pigmented epithelial structures. [17]. Iris stroma develops as a result of neural crest cell migration – within this stroma, the dilator and constrictor muscles develop from the neuroectoderm of the optic cup. In the anterior of the eye, the optic epithelium is non-neural and matures as ciliary body and iris epithelia [17].


The cornea is the unique, transparent and avascular component located at the most anterior section of the eye. It is composed of five layers stemming from the 3 main layers of epithelium, stroma and endothelium:

  • Corneal epithelium (most anteriorly) – consisting of multiple layers of cells which rest on a basement membrane.
  • Bowman’s membrane – a clear, acellular homogenous layer. This layer is a modified portion of the stroma and has no regenerative capacity as a response to damage [18].
  • Corneal stroma – largest portion of the retina consisting of collagenous tissue.
  • Descemet’s membrane – a homogenous, elastic true basement layer with a regenerative capacity.
  • Corneal endothelium – a single layer of cuboidal cells responsible for the maintenance of the dehydrative nature of corneal tissue. Cite error: The opening <ref> tag is malformed or has a bad name.

Corneal development involves interactions between ectoderm-derived tissues. More specifically, the corneal epithelium arises from interactions between the cranial ectoderm and optic vesicles derived from the neural ectoderm. [19]. The neural tube and the adjacent ectoderm give rise to the neural crest cells which are multipotent with a migratory capacity. These cells contribute to the formation of corneal epithelium and the stromal cells. Innervation of the cornea stroma and epithelium is derived from both the neural crest and the ectodermal placode Cite error: The opening <ref> tag is malformed or has a bad name. The endothelium is comprised of differentiated neural crest cells which contribute to the later formation of Descemet’s membrane [20].

Aqueous Chambers

There are two aqueous chambers of the eye: anterior and poster. These chambers are filled with a plasma-like fluid called aqueous humour and is produced by the ciliary body and trabecular meshwork in 3 main stages: diffusion, ultrafiltration and active secretion [21]. The main function of this fluid is to maintain the intraocular pressure as well as provide structural integrity to the round shape of the eyeball. This structure also has a role in the immune response and provides nutrition to the avascular tissue (e.g., the cornea and lens). [22].

The anterior chamber is defined as the developed space posterior to the cornea (between the cornea and the iris) and develops at the stage of differentiation of the mesodermal portion of the iris [17]. The space defining the posterior chamber develops as the mesenchyme between the lens and the peripheral iris.

Choroid and Sclera

The choroid and sclera are both surround the eye and are located adjacent to each other (the choroid lines the inner portion of the sclera). The sclera is the outer, fibrous white layer of the eye which functions as a supportive wall of the eye ball and is continuous with the cornea Cite error: The opening <ref> tag is malformed or has a bad name . The heavy vasculature of the choroid provides a major supply of oxygen and nourishment to the retina [23]. They are derived from interactions between the neural crest and mesoderm-derived mesenchyme. The sclera forms as a result of condensation of the mesenchyme outside the optic cup [24]. The choroid arises from the mesenchyme surrounding the optic vesicle and the cranial neural crest cells. The first vasculature structures appear in the choroid during week 15 in the form of arterioles and venules; veins and arteries become distinguishable by week 22 [25].

Extraocular muscles

The extraocular muscles are responsible for eye movement within the eye orbit – a critical aspect of sight and communication. 6 main muscles contribute to eye movement. These include: superior rectus, inferior rectus, lateral rectus, medial rectus, superior oblique and inferior oblique.

The development of these muscles is dependent on interactions between the eye, its muscles and the neural crest. [26]. Somites supplied by cranial nerve III forms 5 of the 6 extraocular muscles which form an interlocking tendinous ring made of fibrous connective tissue. The 5 muscles include the lateral, medial, superior and inferior rectus, and the superior oblique muscle. This common tendinous ring is known as the annulus of Zinn and surrounds the entry of the optic nerve. The remaining sixth muscle is the inferior oblique muscle and is separate from the aforementioned tendinous ring due to its distinct point of origin. [27].


The eyelids are upper and lower folds of skin functioning as a barrier protecting the anterior surface of the eye from direct local injury. Eyelids also regulate light exposure, distribute precorneal tear film over the corneal surface when blinking and are involved in functions involving tear flow [28]. No sign of eyelid fold development is evident at week 5 however at this stage the lens pit begins to invaginate from the surface ectoderm [29]. Week 6 initiates the timeline of eyelid development as small depressions emerge in the surface ectoderm both above and below the developing eye – these rapidly deepen to form the folds of the eyelids [30]. The lower eyelid fold develops first and, by the end of week 6, the lower fold is more distinct than the upper eyelid fold [31]. Week 7 marks the point at which both the upper and lower folds are distinctly developed and during this stage, the upper lid assumes its more dominant role [29]. The eyelid is not fully closed - there is a gap between the folds and the cornea is slightly visible – they eventually undergo fusion in week 8 [29].

Lacrimal Glands

The lacrimal glands are exocrine glands located in the upper lateral region of each eye orbit which secrete the aqueous potion of the tear film. This film is continuously secreted and functions to clean and protect the external surface of the eye, keeping the environment constantly moist and preventing possible injury from dust and other particles [32]. Lacrimal gland morphogenesis is identified in three stages.

  • The first is the presumptive glandular stage. This stage involves the thickening of the conjunctival superior fornix epithelium and the condensing of the surrounding mesenchyme [33].
  • The second is the bud stage. This stage is considered the initial sign of glandular formation due to the development of nodular structures and lumina within epithelia buds [33].
  • The third is the glandular maturity stage (week 9-16). In this stage, the lacrimal gland begins to morphologically mirror the adult gland [33].

Signalling pathways and transcription factors involved throughout eye development

Eye field development

Many signalling pathways and transcription factors are involved in the development of the eye field (which forms in addition to the telencephalon and diencephalon as part of division of the anterior neural plate). Eye progenitor cells are guided into the eye field region by fibroblast growth factor, bone morphogenetic protein and Wnt-signalling pathways [34]. Additionally, interactions between canonical and non-canonical Wnt-signalling have been shown to maintain the eye field border [35].

The cells of the eye field express eye field transcription factors, some of which include Rx1/Rax, Pax6, Lhx2, Six3 and Otx2. Six3 ,Pax6, Otx2 and Rx1 specify retinal lineage and regulate progenitor cell movement to guide them to their correct locations [34]. Six3 also partly mediates Wnt-signalling repression, allowing for the development of the optic vesicles from the eye field [36]. Lhx2 maintains optic lineage in eye progenitor cells and suppresses the potential for differentiation towards different fates [37].

Eye field division and progression to optic cup

Tgf-β, Fgf and Shh family factors secreted by the axial mesoderm underlying the eye field initiate its splitting into two bilateral, symmetric eye primordia [36]. These factors also regulate the expression of transcription factors Pax2, Pax6, Vax1 and Vax2, thereby establishing the boundaries and patterning the proxiodistal and ventronasal axes of the optic vesicles [34].

Despite the close relationship between cellular proliferation and morphogenesis, optic vesicle evagination does not appear to be mainly driven by proliferation [36]. Alternatively, it has been shown that retinoic acid has critical importance in optic vesicle evagination to form the optic cup [34]. Rx3 activity has also been observed to have involvement in optic vesicle evagination [36].

Eye formation in the absence of the retina

The gene Rx is a key component in the formation of retinal structures. Studies have shown that mice lacking Rx function do not form optic sulci or optic vesicles and do not have retina-specific gene expression. There has been made studies in human, medaka, zebrafish and Xenopus which suggest that Rx genes are required for the formation of the vertebrate retina. It is also shown that in Rx-deficient mouse embryos the mature lens does not develop. This indicates that retinal cells are necessary for lens formation, and if the mouse is lacking Rx gene, then it will not display any retina-specific gene expression [4].

It is thought that signaling from the optic vesicle is important to activate the lens-specific gene and the formation of the lens placode. If there are no retinal cells generated, then the morphogenesis of the optic cup does not take place and the lens will not form. The study also showed that elimination of β-catenin expression in the head surface ectoderm in Rx-deficient embryos would develop a lens-like structure even though there were no optic vesicle/cup. Elimination of β-catenin lead to upregulation of Pax6, which is a gene that has a key role in lens formation. This indicates that the optic vesicle/cup might not be required for lens formation. The lens developed smaller than in wild type embryos, which means that the optic cup/vesicle might have an effect on a determination of the size of the lens. It could be that the developing optic vesicle/cup shields the developing lens placode from signals for other tissues that could disturb the lens formation [4].

It is not fully understood if the formation of the auxiliary eye structures is dependent on the development of the eyeball and its components, the retina and the lens. A study made my Eric C. Swindell and al. showed that auxiliary eye structures form even in the absence of retinal morphogenesis and retina-specific gene expression. This indicates that there are two separate developmental processes involved in the formation of the eye and its associated structures. In the absence of the retina and the lens, we still see specific gene expression in the surface ectoderm initiating the formation of auxiliary eye structures like eyelids and lacrimal glands [4].

Current research

Iris epithelium cells as a potential source for regenerative medicine for retinal pigment epithelium degeneration

The retinal pigment epithelium (RPE) is a monolayer of neural-crest-derived cells and is located between the photoreceptors and the choroid. There are many disorders that involve dysfunctional RPE and leads to retinal degenerative diseases. There is no treatment for these diseases, therefore, one of the most promising future therapy for RPE related disorders is to replace the cells of the dysfunctional RPE [38]..

Studies have focused on the development and use of induced pluripotent stem cells (iPSC) for cell replacement therapy. It is possible to differentiate iPSC in vitro towards RPE cells and use them for experimental transplantation studies in animal models. But an alternative strategy for a retinal replacement is based on transdifferentiation (direct conversion) and involves transforming an adult somatic cell into another adult somatic cell. Use of overexpression of cell-lineage specific genes can convert one cell into another cell type and skip the pluripotent state. Iris epithelium (IE) cells might be able to be transdifferentiated into the RPE since both RPE and IE are neuro-epithelia and has a common embryological origin - they both derive from neuroectoderm of the developing optic cup [38].

A study made by Anna Bennis et al. [38]. has shown that the canonical pathways for the most highly expressed genes of the IE and the RPE were very similar. Wnt signaling pathways has shown to be active in the IE but not in the RPE. The Wnt signaling pathway consists of a group of signal transduction pathways that has an influence on cell fate determination and cell proliferation during embryonic development. Activation of Wnt SP makes it possible to reprogram somatic cells (also retinal neurons) into iPSC and is important for the differentiation of pluripotent stem cells to RPE cells. The high expression of Wnt SP genes in the iE suggests that the IE has a multipotent character during life and therefore could be transformed into RPE cells used for RPE replacement [38].

Animal Models

Congenital Abnormalities

Although quite rare, abnormalities can occur during the embryonic development that causes serious effects to the features of the eyes, its function as well as the further normal development after birth. These abnormalities can occur unilaterally or bilaterally and it is also common for most reported cases to have more than one type of eye abnormalities at a time. Thus, the estimated prevalence statistics listed in the table can overlap.

Congenital Anomalies Description Epidemiology
Anopthalmia The absence of the eyes. Prevalence: Anophthalmia-Microphthalmia Syndrome was estimated to be 5.3 per 100 000 cases [39]

Cause: SOX2 gene mutation [40]

Microphtalmia Abnormal reduction in size of the eyeballs.
Coloboma A condition where parts of the eye is missing and failed to develop normally. Prevalence: Occular coloboma was estimated to be 8.0 per 100 000 cases [39]
Aniridia Complete or partial absence of the iris. Prevalence: Estimated to be 1.5 per 100 000 cases [39]
Optic Nerve Hypoplasia A condition where the optic nerves are underdeveloped. Prevalence: Reported to be the most common abnormality [41]
  • 10.9 per 100 000 in United Kingdom
  • 17.3 per 100 000 cases in Sweden.
  • 6.3% of cases was reported in New Zealand

Future questions

Will it be possible to have eye transplants in the future?

Lots of research has been done in making a whole-eye transplantation successful in order to finally cure blindness. Blindness is a worldwide problem and is currently untreatable due to the fact that retinal ganglion cells and the optic nerves do not regenerate. This can result from diseases including macular degeneration and end of stage glaucoma. The first successful eye transplantation was led by Stone and Cole in 1943[42] investigating 104 samples of the salamander species, Amblystoma Punctatum, and one of its eyes were either implanted back into the same animal or it was completely transplanted to a new host. It was reported that circulation in the iris was re-established, retina was able to regenerate, a new optic nerve was connected to the brain through the optic chiasma and ocular movements were regained. Vision was reestablished by the second month. In a more recent study, in 2015, Li et al.[43] investigated the feasibility of eye transplantation by observing the surviving 15 rat models out of the original 22. They were able to confirm the integrity and functional return of vision after whole-eye surgeries with the aid of advance techniques that are available today. The Ear and Eye Foundation of Pittsburgh[44] is an organization that is currently running a project with lead researchers to discover a way for the optic nerves to regenerate and to cure blindness by 2020.

Will the bionic eye pass clinical trials in the next few years?

The bionic eye is an external visual device that has been in the making for the last several years. It hopes to bring back full vision through the aid of a pair of glasses with a camera attached to it, which then transmits high-frequency radio signals to the microchip implanted in the users' eye. These signals are converted into electrical impulses by the electrodes implanted in the chip, which then stimulates the retinal cells and passes through the optic nerve. Electrical impulses travels to the vision processing centres in the brain and these are then interpreted as an image. At the moment, users must learn to understand light flashes and visual patterns and convert them into images they can interpret. Current prototypes require the users to have a previously working eyes, which means their optic nerves must still be relatively healthy and their visual cortex must be developed. Patients who have suffered from retinitis pigmentosa and age-related macular degeneration would benefit this technology. Bionic Vision Australia[45] is one of the leading organisations that are aiming to treat blindness and are set to perform clinical trials on two of the devices of the bionic eye implants soon. Their objectives are to develop strategies that can improve the affected visual pathway to the brain by stimulating the remaining working retinal cells and also develop safe surgical procedures.


Term Definition
Age-related macular degeneration An eye disease with its onset usually after age 60 that can progressively destroy the macula, the central portion of the retina, impairing central vision.
Aqueous humour A transparent fluid similar to plasma with low protein concentrations secreted by the ciliary epithelium.
Choroid The pigmented vascular layer of the eye located between the retina and sclera.
Ciliary body The connection between the iris and choroid consisting of the ciliary muscle, ciliary processes and the ciliary ring. Alters the shape of the lens and ciliary epithelium in order to focus on an image.
Cornea The transparent anterior part of the external coat of the eye covering the iris and pupil and continuous with the sclera.
Ectoderm The outermost primary germ cell layer in the early embryo.
Endoderm The innermost primary germ cell layer in the early embryo.
Ectodermal placode A neurogenic placode consisting of an area of thickened epithelium in the embryonic head ectoderm layer that gives rise to neurons and other structures of the sensory system.
Extraocular muscles Muscles controlling eye movement.
Fovea A small depression in the retina where visual acuity is the highest.
Intraocular pressure Fluid pressure inside the eye created through continued renewal of fluids.
Iris The coloured muscular ring surrounding the pupil which manipulates the diameter and size of the pupil and controls the amount of light entering the eye.
Lens The transparent biconvex structure posterior to the iris with the main function of focusing light onto the retina in order to form clear images of objects at various distances.
Lens pit A pit-like depression in the fetal head where the lens develops.
Lens placode The placode developing in the ectoderm overlying the optic vesicle, eventually becoming the lens of the eye.
Lens vesicle The ectodermal invagination in the embryo formed opposite the optic cup and gives rise to the lens.
Lumina the central cavity of a tubular structure.
Macula An oval-shaped pigmented area where there is the largest density of cone cells and is involved in producing the sharpness of central vision.
Mesenchyme Loosely organised embryonic connective tissue originating from the mesoderm.
Neural crest A temporary group of embryonic ectodermal cells.
Neural retina Refers to three layers of neural cells within the retina (photoreceptor cells, bipolar cells and ganglion cells).
Neural tube A hollow tubular structure as a result of neural plate folding which later develops into the brain and spinal cord.
Neuroepithelium Epithelium consisting of specialised cells which detect sensory triggers for reception of external stimuli.
Optic cup A structure of the diencephalon formed after optic vesicle folding. This structure gives rise to the retina.
Optic groove Two small grooves on each side of the developing forebrain in the neural folds. Also chiasmatic groove.
Optic nerve Cranial nerve II which transmits impulses to the brain from visual information detected by the photoreceptive retina.
Optic stalk Pair of slender embryonic structures that give rise to the optic nerve.
Optic vesicle An evagination of each lateral all of the embryonic vertebrate forebrain from which the nervous structures of the eye develop.
Pupil The central opening in the iris, allowing and regulating the entry of light into the eye.
Retina The sensory membrane that forms the inner lining of the eye containing rods and cones with the main function of photoreception. Nerve impulses in response to visual information are transmitted to the brain via the optic nerve.
Retinitis Pigmentosa A chronic hereditary eye disease characterized by breakdown and loss of cells in the retina leading to progressive visual loss.
Sclera The dense, white, fibrous membrane that, along with the cornea, forms the external protective covering of the eye
Tear film A layer of tears which spreads over the eye, ultimately maintaining the health of the ocular surface by making the external surface smooth and clear.

Glossary Links

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External links