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The primordial brain initially has the same basic structure as the developing spinal cord, however, consideration variations in the outline of transverse sections at different levels of the brain and in the position of the gray and white matter are produced by the brain flexures <ref name="textbook"/>. The sulcus limitans (the floor of the fourth ventricle) extends cranially to the junction of the midbrain and forebrain, and the alar and basal plates are recognisable only in the midbrain and hindbrain <ref name="textbook"/>.
The primordial brain initially has the same basic structure as the developing spinal cord, however, consideration variations in the outline of transverse sections at different levels of the brain and in the position of the gray and white matter are produced by the brain flexures <ref name="textbook"/>. The sulcus limitans (the floor of the fourth ventricle) extends cranially to the junction of the midbrain and forebrain, and the alar and basal plates are recognisable only in the midbrain and hindbrain <ref name="textbook"/>.
[[File:Developing brain.pdf|400px|center]]
[[File:Brain Development.png|400px|center]]
[[File:Neural- cortex Cajal drawing 01.jpg |thumb|right|200px|Laminar and columnar organization of the cerebral cortex (Historical drawing by Cajal)]]
[[File:Neural- cortex Cajal drawing 01.jpg |thumb|right|200px|Laminar and columnar organization of the cerebral cortex (Historical drawing by Cajal)]]

Revision as of 12:06, 25 October 2017

Cerebral Cortex

2017 Student Projects 
Student Projects: 1 Cerebral Cortex | 2 Kidney | 3 Heart | 4 Eye | 5 Lung | 6 Cerebellum
Student Page - here is the sample page I demonstrated with in the first labs.I remind all students that you have your own Group Forum on Moodle for your discussions, it is only accessible by members of your group.
Editing Links: Editing Basics | Images | Tables | Referencing | Journal Searches | Copyright | Font Colours | Virtual Slide Permalink | My Preferences | One Page Wiki Card | Printing | Movies | Language Translation | Student Movies | Using OpenOffice | Internet Browsers | Moodle | Navigation/Contribution | Term Link | Short URLs | 2018 Test Student


Fig.1. Cerebral cortex is the outermost layer of the cerebrum [1]

The cerebral cortex surrounds the cerebrum, and is the largest part of the human brain. It is thought to be the main control centre, playing an essential role in cognitive function, memory, sensation and association.The cerebral cortex differs from the cerebrum because, the cerebral cortex is the outermost layer of the cerebral hemispheres; it is around 2-4 mm in thickness, consists of the layer of grey matter and has ~ 10 billion nerve cell bodies and dendrites.

Fig 2. Section of the human brain [2]

The development of the cerebral cortex involves 3 main stages: proliferation/growth/differentiation, migration of neurons, and maturation/organisation/folding. The cortex is organised into six horizontal layers. I. Molecular layer, II. External granular layer , III. external pyramidal, IV. internal granular layer, V. internal pyramidal layer, VI. multiform layer. These individual layers organise the capacity for interconnections between both input and output signals.

Early Development of the Brain

The brain begins to develop during the third week of pregnancy when the neural plate and neural tube are derived from the outer most layer of embryonic cells, that is the neuroectoderm. The development of the brain is from the neural plate which folds to form the neural groove and then curls forming the neural tube, which is cranial to the fourth pair of somites, and eventually, forms the three primary brain vesicles [3].

Neuroprogenitor cells proliferate, migrate, and differentiate to form specific areas of the brain [4].

During week four fusion of the neural folds in the cranial region and closure of the rostral neuropore form three primary brain vesicles from which the brain develops [3]:

  • Forebrain/Prosecephalon
  • Midbrain/Mesencephalon
  • Hindbrain/Rhombencephalon

From the three primary vesicles, there is a further division at the anterior extremity of the medullary canal into five secondary vesicles during week five. These are fundamental divisions of the adult brain and communicate freely with each other [5]. They are [4]:

  • Telencephalon: endbrain, forms cerebral hemispheres - derived from Prosecephalon
  • Diencephalon: between brain, forms optic outgrowth - derived from Prosecephalon
  • Mesencephalon: undivided
  • Metencephalon: posterior to the brain, gives rise to the pons (bulbous expansion consisting of white matter tracts serving the cerebellum) [6] - derived from Rhombencephalon
  • Myelencephalon: gives rise to the medulla oblongata - derived from Rhombencephalon

In the beginning, these five divisions are uniform in size and shape but quickly differentiate at various rates [5].

As the telencephalon is the region that develops into the cerebral cortex, we will discuss this region specifically in more detail. The telencephalon is the utmost rostral region of the brain vesicles, and consists of:

  • The median region: the lamina terminalis, with the cavity forming the anterior part of the third ventricle [7]
  • Two lateral diverticula: the cerebral hemispheres [4]

The cerebral hemispheres grow simultaneously in lateral, longitudinal and parietal directions [7], and originally are in communication with the third ventricle cavity via the interventricular foramina [4]. Upon expansion, the cerebral hemispheres eventually cover the diencephalon, midbrain and hindbrain, where they will eventually congregate at the midline, resulting in the flattening of each hemispheres medial surfaces [4]. During the sixth week the corpus striatum emerges as an obvious swelling in the floor of both of the cerebral hemispheres. the floors expand at a slower rate compared to the hemispheres thin cortical walls, as it contains large crpus striatum, causing the cerebral hemispheres to become a c shape [4]. This growth and curvature of the cerebral hemispheres does have consequential effects on the shape of the lateral ventricles, which too become c-shaped and fill with cerebrospinal fluid (CSF). Each hemispheres caudal ends turn ventrally, and then rostrally resulting in the formation of the temporal lobe. The telencephalon is further subdivided into a dorsal pallium and a cenrtral subpallium. The dorsal pallium forms the large neuronal nuclei of the basal ganglia (corpus striatum, globus pallidus) [6]. These structures are essential for fulfilling commands from the cerebral hemispheres, and appear as lateral outpouchings of the pallium growing at a fast rate in order to cover the mesencephalon and diencephalon. The cortex is formed by the pallium, or vault, of each hemisphere.

Brain Flexures

During the fifth week, the embryonic brain undergoes rapid growth, folding the neural tube, consequently resulting in three brain flexures. These are [3]:

  • Cephalic flexure - centered at the midbrain region and pushes mesencephalon upwards being the first fold to develop, ventral folding of the brain tube [6]
  • Cervical flexure - between brain stem and spinal cord at the junction of the spinal cord and hindbrain, ventral folding of the brain tube [6]
  • Pontine flexure - beings at the developing pons, generates the fourth ventricle; produced in the opposite direction - dorsal folding [6] - as a result of later unequal brain growth, resulting in the thinning of the roof of the hindbrain [5].

The primordial brain initially has the same basic structure as the developing spinal cord, however, consideration variations in the outline of transverse sections at different levels of the brain and in the position of the gray and white matter are produced by the brain flexures [4]. The sulcus limitans (the floor of the fourth ventricle) extends cranially to the junction of the midbrain and forebrain, and the alar and basal plates are recognisable only in the midbrain and hindbrain [4].

Brain Development.png
Laminar and columnar organization of the cerebral cortex (Historical drawing by Cajal)

Development of the Cerebral Cortex

Corticogenesis refers to the development of the cerebral cortex, the outer portion of the cerebrum, into its six layers. It involves a sequential process of migration and differentiation of neocortical projection neurons into a laminated structure that contains a diverse set of neuronal subtypes. The diversity in neurons contributes to the complex circuitry involved in the mammalian cortex. The large size of the cortex distinguishes humans from other mammals because it corresponds to a larger capacity to perform behavioral and cognitive tasks. [8] As mentioned in above in “Early Development of the Brain,” the cerebral cortex is derived from telencephalon, the rostral end of the neural tube. Origins of various neuronal subtypes come from the pallium (roof) and the subpallium (base) sections of the telencephalon which contributes to the overall laminar and columnar organization of the cortex. [9]

getting refererence [10]

Main classes of neurons [11]

  • Projection neurons: excitatory neurons (glutamatergic) with axons that project to distant targets and are generated by progenitors in the dorsal pallium of the telencephalon. The have a typical pyramidal structure and send signals to various regions of the brain and neocortex.
  • Interneurons: inhibitory neurons (GABAergic) that have local connections within the cortex and are generated in the subpallium of the telecephalon in the ventral proliferative zone. These neurons have to migrate to the neocortex area.
Key developmental zones in the human cortex

Key developmental zones in the human cortex:

  • Ventricular zone
  • Subventricular zone
    • Inner Subventricular Zone
    • Outer Subventricular zone
  • Intermediate Zone
  • Preplate: neural progenitor cells split into 2 regions
    • Marginal zone
    • Subplate
  • Cortical Plate: establishment of the 6 cortical layers form in this region between the subplate and the marginal zone

Timeline of Corticogenesis

The dorsolateral wall of the telencephalon is initially made up of undifferentiated neuroepithelial cells at the beginning of development. The cells are neural stem cells, capable of dividing into various neuronal subtypes. [8] The timing of birth for these neuronal subtypes determines the location (e.g fate) of the neurons so that specific connections and cortical structure are achieved. "E" relates to embryonic day in relation to corticogenesis.

E30 Progenitors in the dorsal telencephalon divide symmetrically and give rise to the initial ventricular zone (VZ), a single layer of cells that attach their "feet" to the cerebral wall and divide. These neurons lay adjacent to the ventricular surface. [8]
E31-32 Dividing cells from the VZ begin to differentiate into "pioneer" neurons to form the preplate. These neurons migrate radially and tangentially from the VZ to the pial surface in an upward fashion. These cells are often referred to as radial glial cells (RGCs) because they contain radial fibers that span the entire embryonic wall from the VZ to the pial surface. These fibers create a "scaffolding" for subsequent migrating neurons to attach to and travel along in order to expand the neocortex. These cells are multipotent cells that divide asymmetrically to produce intermediate precursor cells that can become projection neurons, astrocytes, and oligodendrocytes [12] . In addition to RGCs, Cajal-Retzius (CR) cells are another type of early born neurons that develop at the same time as the preplate. These cells express reelin, an important signaling factor for migrating neurons to properly organize into their destined layers. They remain in the marginal zone when the preplate splits into two (see E50-55).[9] The preplate is referred to as heterogeneous because it contains many developing cell types.
E40-45 Cells in the VZ continue to divide symmetrically and give rise to another zone known as the subventricular zone (SVZ). This proliferative layer lies above the ventricular zone and is not attached to the ventricular surface. Radial glial cells also populate this area as well as the preplate and many of the cells in the SVZ contribute to the later cortical neurons. The SVZ also separates into the outer subventricular zone (OSVZ) and the inner subventricular zone (ISVZ). The OSVZ continues to expand as it produces more migrating immature neurons and intermediate precursor cells. [12]
E50-55 The preplate begins to split into two subsections: the marginal zone and the subplate. An intermediate zone forms below the subplate and contains only migrating cells (migrating to the target destination) and no intermediate precursor cells. The cortical plate also begins to form in between the two regions [11]. Newly born neurons that migrate to the cortical plate are developed "inside out". This term "inside-out" means that earlier born cells populate the deep layers first and later born neurons populate the superficial layers of the neocortex by migrating past the deep layers. Therefore, layers 6 is populated before layer 5; layer 5 is populated before layer 4 and so on [8]. Each layer condenses and the neurons are closely packed. As the layers form, the cortex expands.

Migration and division of all six layers of the cortex is completed during the third trimester.[8] Each layer has distinct synaptic connections and cell types that contribute to the specific functions of the cortex such as sensory and motor function.

Corticogenesis from E30 to adult human brain

Signalling involved in Cerebral Cortex Development

Figure 1: Shh signalling increases the number of proliferating cells

Cell signalling is an essential part of the laminar patterning of the cerebral cortex into its 6 distinct, radially organised layers. There is a large network of interweaving signalling pathways that are responsible for the formation of this sophisticated anatomical structure and its functioning. There is still much debate about the exact pathways and signalling molecules that lead to this specific patterning, however some factors contributing to the control of cortical differentiation have been uncovered.

Figure 2: Cortex development in wild-type and reeler mice [13]

Sonic Hedgehog (Shh) is a morphogen involved in regulating the development of many different tissue types. During the development of the neocortex, Shh plays a role in controlling the proliferation and survival of cortical progenitor cells along with the specification of cerebral cortex GABAergic neurons. [14]

It has been shown that blockade of Shh in murine models with cyclopamine has effects on cortical neurogenesis, with indications that Shh morphogen could be play a role in the control of cell cycle length, cell growth and the process of cell division of the dorsal telencephalon progenitors during early corticogenesis (Fig. 1) [15]

Reelin is one of the more well understood signalling pathways involved in corticogenesis. Cajal-Retzuis cells with are located in the marginal zone (MZ) secrete Reelin (an extracellular matrix glycoprotein). Through studies that examine the absence of Reelin in reeler mutant mice, neuronal migration is significantly altered (Fig. 2). Additionally, Reelin has been shown to have a crucial role in inducing branching and specific positioning of radial glial cells (RGC), in that the distribution of Reelin within the MZ defines the specific location of radially migrating neurons, and is essential for the characteristic ‘inside-out’ pattern of the six cortical layers. [16]

Notch signalling molecular pathway is also crucial to corticogenesis. It is thought that there is an important interplay between this pathways and Reelin, as deficits of both result in impaired migration and altered morphology. [17] Both signalling pathways are involved in the expression of the radial glial gene, BLBP, which could be significant in the development of radial glial cells. 5. Although generally considered a developmental pathway, studies have shown the importance of Notch signalling in the adult brain, through distinguishing the balance between maintenance of neural stem cells and differentiation. These new understandings have implications for therapeutic approaches to neurodegenerative diseases. [18]

Figure 3: Cortical thickness in the absence of Bmp7[19]

Bmp7 in addition to cell intrinsic factots there are also estristic signalling factors to take into account, for example Bone Morphogenitic Protein 7 (Bmp7) with debate ongoing as to its promotion or inhibition of neurogenesis. Deletion of Bmp7 in murine models has been shown to result in reduced thickening of the cortex, likely due to the changed differentiation of progenitor cells from the subventricular zone to the upper layers. Bmp7 regulates the expression of gene Ngn2, which in Bmp7 knock out models appeared to be majorly reduced, perhaps playing a role in the development of deep layer neurons. [20]

The signalling pathways that underlie corticogenesis are very complicated and the exact mechanisms are still under continuous investigation. Further research will only improve understanding of this network of intertwining factors and potentially lead to better appreciation of neurodevelopmental conditions and thus innovative therapeutic approaches.

Anatomy of the Cerebral Cortex

The cerebral cortex is a convoluted layer of grey matter on the outer surface of the cerebrum. Grey matter is neuronal tissue containing neuronal cell bodies. In humans the cortex has a thickness of 2-3mm however it's surface area is many hundred square centimetres allowing an increased cortical surface to occupy small cranial volume. The cortex in humans contains 10 billion densely packed nerve cells (10% of the neurons in the brain). The human neocortex is the most phylogenetically developed structure of the brain compared to other species. The folding in larger mammals is important to allow addition and evolution of a greater diversity of functional areas. As the folding is highly preserved across individuals this allows the different sections sepearted by gyri and sulci to be labelled. [21]

Anatomy of the human cerebral cortex [22]

The longitudinal fissure divides the cortex into the left and right hemispheres, which are connected at the midline by the longitudinal fissure. Each hemisphere is then divided into four lobes (frontal, temporal, parietal and occipital) which are labeled by their relation to bones of the skull. The frontal lobe is separated from the temporal lobe by the lateral sulcus also known as the Sylvian fissure. The Rolandic fissure (central sulcus) divides the frontal and parietal lobe and the parietal and occipital lobes are distinguished by the parieto-occipital sulcus. [23]

The frontal lobe is subdivided by the superior and inferior frontal sulci into the superior, middle and inferior frontal gyri. The frontal operculum is formed by the inferior frontal gyrus and is divided into the pars orbitalis, pars triangularis and pars opercularis. These are triangular gyri and are listed in order of anterior to posterior. [21]

The temporal lobe can also be subdivided by the superior and inferior temporal sulci. These subdivisions include the superior, middle and inferior temporal gyri. Lateral to the midbrain on the inferior temporal lobe surface is the parahippocampal gryus. The occipitotemporal gyrus, also named fusiform gyrus, lies between the inferior temporal gyrus and the parahippocampal gyrus. Within the parietal lobe the angular gryrus lies above the superior temporal sulcus. Above the angular gyrus, the supramrginal gyrus lies above the lateral sulcus. Below the angular gyrus, the lateral occipital gyrus lies above the inferioir temporal sulcus. [21]


Layers of the Cortex

Layer 1

outermost layer (pial surface)

molecular layer with few scattered neurons

mainly extensions of pyramidal neuron apical dendrite tufts with some spiny stellate cells

inputs to apical tufts are crucial for feedback interactions in cortex in associative learning and attention [24]

Layer 2

external granular layer

contains small pyramidal neurons and many stellate neurons

pyrimidal neurons are excitatory [25]

Layer 3

external pyramidal layer

small and medium sized pyramidal neurons

non-pyramidal neurons with orientated intracortical axons

layers 1,2 and 3 are the main target for interhemisphere corticocortical afferent fibres

layer 3 is the main source for cortiocortical efferent fibres

Layer 4

internal granular layer

many different types of stellate and pyramidal neurons

main target for thalamocortical afferents from thalamus type C neurons and intra-hemispheric corticocortical afferents [26]

Layer 5

internal pyramidal layer

large pyramidal neurons

give rise to axons leaving cortex and run down to subcortical structures e.g. basal ganglia

In the primary motor cortex of the frontal lobe, layer 5 contains Betz cells and their axons travel through the internal capsule, the brain stem and the spinal cord forming the corticospinal tract, which is the main pathway for voluntary motor control

cortical areas with no layer 5 are named agranular and cortical areas with an undeveloped layer 5 are named dysgranular [25]

Layer 6

polymorphic/ multiform layer

few large pyramidal neurons

many small spindle like pyramidal and multiform neurons

sends efferent fibres to thalamus and forms exact reciprocal interconnection between thalamus and cortex

these connections are both inhibitory and excitatory [27]

Functions of the Cerebral Cortex

The cerebral cortex controls memory, movement, perception, cognition, consciousness, awareness and language. Majority of the connections in the cortex are from one cortex area to the other rather than with other structures. However, the cortex is connected to structures below it such as the basal ganglia and thalamus. The cortex communicates with these structures; information is sent along efferent connections and received by afferent connections. Majority of the sensory information in the brain is sent to the cortex by the thalamus and olfactory information is send to the olfactory cortex through the olfactory bulb. The cortex can be split into three cortical areas (cortices for plural) based on their function; sensory, motor and association areas. [28]

Cortical areas human cortex [29]
Cortical Homonculus [30]

Primary cortices have simpler functions including direct sensory input (vision, hearing and somatic sensation) or directly producing eye and limb movements. The association cortices functions are more complex and include memory, language, abstraction, creativity, judgement, emotion, attention and movement synthesis. [28]

Sensory areas receive and process information from the senses including visual, hearing and somatosensory information. The primary sensory areas receive sensory inputs from the thalamus. The sensory area in each hemisphere receives sensory information from the opposite side of the body. The sensory cortex is organised as shown in figure and provides a map of the body also known as a homunculus. A cortical homunculus is a model used to represent the area and proportions of motor and sensory functioning in the human body. The size of the body part represents the innervation density, for example the fingers and lips have a higher number and more complex sensory and motor connections. They require more cortical area for the processing of finer sensation. [31]

The motor areas control voluntary movements and like the sensory areas, the motor area in the left hemisphere controls the movement for the right side of the body and vice versa. The basal ganglia receive input from the motor areas as well as the midbrain (substantia nigra) and also sends signals back to these areas aiding the control of motor movements. [32]

The association areas produce perceptual experience and involve functions such as abstract thinking and language. The parietal, temporal and occipital lobes assimilate information stored as memories and sensory information. The association areas connect distant areas of the cortex. [28]

Cortical Area Function
Primary motor cortex Executes voluntary movement
Primary visual cortex Receives and processes visual information
Primary somatosensory cortex Receives and processes somatosensory information such as heat, pain and pressure
Primary auditory cortex Receives and processes auditory information
Motor association cortex (premotor cortex) Selects voluntary movements
Auditory association area Complex processing of auditory information
Sensory association area Complex processing of multi sensory information
Visual association area Complex processing of visual information
Prefrontal cortex Involved in organising thoughts and actions to match internal goals. These include planning complex cognitive behaviour, making executive decisions, expressing personality, social awareness and storing short term memories. [33]
Broca's area (speech centre) Speech production and articulation [34]
Wernickes area Speech comprehension [34]


Abnormalities associated with Cerebral Cortex Development

Main stages of cortical development where abnormalities may arise [36]

The embryologic development of the cerebral cortex involves highly organised and complex events (as has been seen in the section 'Development of the cerebral cortex').

These events are generally divided by scientists into 3 major stages in cortical formation where crucial changes occur in neuronal cells; these stages include:
1. Proliferation (or Growth),
2. Migration,
3. Organisation (or Maturation or Differentiation).

Disruptions in these stages of cortical development, are the precedent that give rise to malformations or irregularities in the cerebral cortex (Fig. ). This section will explore some abnormalities that are commonly discussed in scientific literature.

Disorders due to the abnormal proliferation, growth or differentiation of neuroblasts

1) Focal cortical dysplasia (FCD)

Focal cortical dysplasia (FCD) is heterogeneous developmental disorder of uncertain etiology. Focal Cortical Dysplasia is characterised by neurons arranged abnormally in the focal areas of the cerebral cortex as well as disorganisation of layers and very large cells called 'balloon' cells.
FCD can affect the areas throughout the brain but occurs dominantly in the frontal and temporal lobes of the cerebral cortex.

In all patients who present with epilepsy, FCD is the cause for about approximately 25% of them. FCD can be of two types: Type I and Type II.

Hemimegalencephaly [36]

2) Hemimegalencephaly

A rare congenital disorder that also results from the abnormal proliferation of neuroblasts is Hemimegalencephaly.

Hemimegalencephaly is a developmental disorder which consists of a 'hamartomatous' overgrowth (where normal mature cells and tissues normally present in an area of the body, form a tumour-like, benign malformation) on part or all of the cerebral hemisphere.

Hemimegalencephaly accounts for 0.2% of cases of childhood epilepsy. Clinical symptoms of this disease may include developmental delay, paralysis of one side of the body and blindness over half the field of vision; but the most significant symptom is seizures as 90% of patients present with this symptom.

Microcephaly [37]

3) Microcephaly Vera

Microcephaly or 'small brain', can be caused by abnormal cell proliferation or cell division along with the involvement of other organs.

Primary Microcephaly or Microcephaly Vera results only due to abnormal cortical development, specifically cell division or proliferation. It is a congenital malformation in which the circumference of the head is quite less than normal and is accompanied by mental retardation and sometimes epilepsy.

References used to write: [36] [38] [39] [40]

4) Tuberous Sclerosis Complex

Tuberous sclerosis complex (TSC) is a multi-organ disease resulting from mutations of certain genes, and is usually classified under defects of early cell proliferation and growth although it is also associated with defects of neuronal migration.

Tuberous sclerosis complex (TSC) is thus called due to lesions seen that resembled potato tubers; and is characterised by benign abnormal mass of cells (tumors, cysts, and other malformations including hamartomas and neoplasms) in the cortex.

" Beneath the cortex, the brain in tuberous sclerosis also shows nodular collections of small cells along the surface of the lateral ventricle that resemble ventricular cells and are called subependymal nodules...or, more descriptively, “candle drippings.” These nodules often contain numerous balloon cells and can in some cases become transformed into glial tumors referred to as subependymal giant cell astrocytomas..."

Disorders due to abnormal neuronal migration

5) Heterotopia

Heterotopia [36]

Migration of neurons occurs during the 8th week of development, along radial glial fibers (RGF). RGFs can be damaged due to ischemia, which can be caused by infection resulting from trauma or metabolic errors.
RGF damage leads to the migration process arresting at the wrong time, resulting in abnormal or ectopic locations of neurons of the cortex. This condition is known as Heterotopia. There are different types of heterotopia:

  • Subcortical band heterotopia:

In this type of heterotopia, ectopic locations of neurons, seen as nodules, occur in the sub-cortical regions of the cerebral cortex.

  • Periventricular heterotopia/ sub-ependymal heterotopia:

In this type of heterotopia, ectopic locations of neurons, seen as nodules, occur in the sub-ependymal or periventricular area of gray matter of cerebral cortex.

  • Focal heterotopia:

In this type of heterotopia, abnormal location of neurons result in focal masses within deep white matter of cerebral cortex.

6) Lissencephaly

Lissencephaly is a congenital cortex malformation, in which gyri (and sulci) of the cerebral cortex are absent (agyria) or largely absent (pachgyria), resulting in a smooth surface of the brain. The normal lamination or layers fail to form and the cerebral cortex usually has only 4 of the typical 6 layers. Like most congenital brain disorders, the etiology of Lissencephaly is uncertain.
Lissencephaly has also been associated with other abnormalities including corpus callosum hypoplasia, small brain stem and gray matter heterotopia.

Lissencephaly is generally characterised by the following features:

  • Total agyria (gyri and sulci absent resulting in 'smooth brain')
  • Pachygyria (gyri can be seen but are very few compared to normal brain)
  • Agyric brain with areas of pachygyria
  • Vertically oriented Sylvian fissures of the brain, giving the brain an 'hourglass' configuration

Lissencephaly is of different types; even so common clinical symptoms are 'epilepsy' and 'severe mental retardation'. Types of Lissencephaly are the following:

  • Classical (Type I) Lissencephaly- results from neuronal undermigration (failed or incomplete neuronal migration) due to varying causes like mutation; additional clinical features include motor deficits. Patients often also have microcephaly [discussed later in Microlissencephaly].

  • Cobblestone (Type II) lissencephaly- results from overmigration, where neurons migrate from the cortex into the overlying leptomeninges, causing diverse disruptions of the basement membrane; the cortex has a 'cobblestone' type of nodular appearance and additional clinical features include various eye abnormalities, congenital muscular dystrophies, hypotonia and generalized weakness in infancy.

Type II Lissencephaly also includes:

  1. Muscle-Eye-Brain Disease

  2. Walker-Warburg Syndrome

  3. Fukuyama Syndrome

  • Microlissencephaly

Microlissencephaly occurs when Type I Lissencephaly occurs in combination with congenital Microcephaly, and presents with severe symptoms including seizures, spasticity, severe developmetal delay and short life span.

7) Kallmann Syndrome

The Kallman Syndrome is syndrome which results from the migration of neurons that secrete leuteinizing hormone-releasing hormone (LHRH) from the olfactory placode to the hypothalamus, causing congenital hypogonadism (since LHRH is necessary for normal gonadal function).

Kallman Syndrome is associated with hypoplasia of the olfactory bulbs and olfactory cortex, called arhinencephaly, and lack of the sense of smell. [not yet changed into own words]

Disorders due to abnormal cortical maturation and organization/folding

8) Polymicrogyria

If the neurons of the cerebral cortex successfully complete the proliferation and migration stages, but during the last organisation stage, distribute abnormally then multiple small undulating gyri can result. This condition is called Polymicrogyria (PMG), in which, the gyri become extremely small (hence the term micro) and their number is greater than normal. The cerebral cortex in this condition is flat and thickened, similar to that of pachygyria or agyria, and contains numerous small gyri.

Polymicrogyria is usually focal. It is most commonly 'perisylvian' (around the Sylvian fissure) and can be 'bilateral' or 'unilateral'. The most common sites, in descending order, are the frontal lobe, parietal lobe, temporal lobe and then occipital lobe.

Schizencephaly [41]

9) Schizencephaly

Schizencephaly is an organisational developmental disorder characterised by a cleft(s) or lesion(s) on the brain surface, which is filled with CSF and lined by gray matter. Schizencephaly can be bilateral or unilateral.

The clefts can be small with closed walls in Type I or Closed lip type schizencephaly; or the clefts can be large with free communication between the ventricle and subarachnoid spaces in Type II or Open lip type schizencephaly.

Schizencephaly commonly involves the parasylvian regions, and severity of the disease correlates with the extent of the clefts.

Other Disorders

12) Fetal Alcohol Spectrum Disorder (FASD)

Facial features appearance in Fetal Alcohol Spetrum Disorder (FASD) [42]

Fetal Alcohol Spectrum Disorder (FASD) refers collectively to the malformations that occur in children due to maternal alcohol consumption during pregnancy. This results from the effects of ethanol as it crosses the placental barrier. The mechanism for these abnormalities developing is complicated and not entirely clear, but various studies show that ethanol disrupts all major processes of fetal CNS development and causes toxicity of blood (as fetal liver cannot yet detoxify ethanol well).

The three major indications (that also help form the diagnoses) for FASD are facial dysmorphology, growth deficits and central nervous system defects. These conditions result in immense physiological and psychological impacts on the child. Fetal Alcohol Syndrome (FAS) is an acute form of FASD.

On average, FASD is diagnosed in a child between 3-10 years. It is of utmost importance however that the diagnosis is done as early as possible so that timely interventions could be taken in order to lessen the severity of the symptoms that result from this syndrome. [43]

13) Corpus Callosum Agenesis



Mouse vs Human Neurogenesis

Models and Research

Animal Models

https://bmcneurosci.biomedcentral.com/articles/10.1186/1471-2202-11-75 (reelin in pig model)

Mice have often been used to study corticogenesis in mammals. Corticogenesis lasts from E11 to E19 in mice and lasts eight days. [46] The importance of reelin has been greatly studied in mice. Studies showed that mutant mice have disruptions in the migration of neurons into their subsequent layers. By injecting thymidine at various time points of gestation into the female mice, researchers were able to track the development of various populations of neurons. Later developing neurons were unable to ascend past the already early formed neurons due to disruptions in reelin signaling (in the marginal zone). Instead, superficial layer neurons resided below the older, deep layer neurons. This inverted lamination comprises sensory and motor function.[47]

Current Research

3D Organoids

Timeline and protocol of cerebral organoid development[48]

One of the limiting steps in research at present is the lack of models that can accurately recapitulate the developmental changes and pathophysiology of the human brain. Although it is recognized that certain fundamentals of brain development are conserved in mammalian brains there are distinct differences that should be considered when studying the human brain. [49]

Currently there has been the development of 3 dimensional models, derived from stem cells that can be used to mimic the evolution of the human brain, and have been deemed advantageous over previous in vitro models. This method, as first described by Lancaster et al. can, in a 1-2 month period be reproduced in a tissue culture room give rise to the developing parts of the human brain, including the cerebral cortex. This is established using a protocol human pluripotent stem cells (PSCs). PSCs separate to single cells before rearranging to form embryoid bodies, which are prompted to form neuroectoderm in a medium that prevents either endoderm or mesoderm development. At day 11-15 of this protocol the tissues undergo neuroepithelial bud expansion after being transferred to Matrigel droplets. The final stage of the process involves the tissues being transferred to an agitator (either spinning bioreactor, or orbital shaking plate) which allows for more substantial growth of the brain regions due to better nutrient and oxygen supply. [50]

Future Questions

http://www.sciencedirect.com/science/article/pii/S0736574804001364 (will most likely use this article)




Term Definition
Sulcus Sulci (plural) are grooves in the cerebral cortex
Gyrus Gyri (plural) are folds/ridges in the cerebral cortex
Fissures Large sulci
Corticogenesis The development of the cerebral cortex into its six layers. It involves a sequential process of migration and differentiation of neocortical projection neurons into a laminated structure that contains a diverse set of neuronal subtypes
Cajal-Retzius (CR) cells Cortical neurons that develop early on, at the same time as the preplate, and express an important glycoprotein known as reelin, which helps with the proper migration of neurons into their respective layers.
GABAergic Neurons Generate gamma aminobutyric acid (GABA) as their output, one of the two inhibitory neurotransmitters in the central nervous system (CNS)
Radial glial cells A class of distinct nonneuronal cells that are bipolar shaped and span the entire width of the cortex during development

INFO/ Research Links (temporary heading)




For concept: https://www.youtube.com/watch?v=dNngOlsLuGI

You might find this helpful (although you need to request copyright permission):

"Difference Between Cerebrum and Cerebral Cortex." DifferenceBetween.Com. August 7, 2012. < http://www.differencebetween.com/difference-between-cerebrum-and-vs-cerebral-cortex/ >

PubMed Searches: Cerebral Cortex Development | Cerebrum Development

BMC Dev Biol Search: Cerebral Cortex Development

Recent papers

<pubmed limit=5>Cerebral+Cortex+Development</pubmed>


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