2012 Group Project 3

From Embryology
Revision as of 03:30, 19 September 2012 by Z3330986 (talk | contribs) (Neural Pathways)

Taste Development

Gustatory System

Introduction

The five basic tastes

Taste or more appropriately gustation, is a fundamental survival tool in animals as it directs the consumption of essential nutrients. The five tastes that exist within the human gustatory system: salty, sweet, sour, bitter and umami, all signify basic physiological requirements. Salty tastes denote the presence of Na+, an important ion involved in the transportation and retention of water across cell membranes. Sweetness is the recognition of carbohydrates, essential for maintaining optimal brain function and providing the basis for energy production in muscle tissue via ATP hydrolysis. Similarly umami is codes for the presence of L-amino acids, especially L-glutamate which is an integral component of protein systhesis.


These different modalities not only function to maintain the proper intake of nutrients within the body but also, by way of aversion, prohibit the consumption of undesired or poisonous materials. The bitter modality for example is generally regarded as displeasing to the human palate. It represents a high acid content which may be a result of foods which have become rotten or are inherently poisonous to the body.


Research has historically limited itself to investigating the mechanisms of these five taste qualities though it must be noted many other modalities may exist. Of particular conjecture is the existence of fatty acid recognition. In the past the detection of fat in food has been attributed to somatosensory inputs from the tongue, that is, the oily feel and texture of fat rather than its actual ‘taste’ allows the brain to encode for its presence. Further research is therefore required to elicit the precise mechanisms of fat detection.

Cell Biology

The five taste qualities are not all detected by the same type of receptor cell located within the taste bud. Like rods and cones in the eye which detect different wavelengths of light, there are specific types of receptors for different tastes. For example, sweet, umami and bitter are recognised by Type II G-protein coupled receptors, whereas sour is related to Type III presynaptic cells. The cell type involved in salty taste transduction is unknown however it is known that sodium ions can enter the receptor cell membrane via ion channel permeation.

Type II receptors

When a bitter, sweet or umami ligand binds to a type II G-coupled receptor, a cascade of chemical reactions causes the release of Ca2+ which in turn mediates exocystosis of ATP. The function of this ATP is threefold:

  1. Stimulates gustatory nerve afferents which project to gustatory nuclei in the spinal cord
  2. Excites Type III presynaptic nerve fibres to release serotonin.
  3. Has a positive feedback effect on type II receptor cells, increasing the level of ATP production.


The role of serotonin is believed to be in the form of lateral inhibition i.e. when a bitter quality is recognised, adjacent receptors for sweetness are deactivated and thus the two tastes may be clearly differentiated. It also has a negative feedback effect on receptor cells, inhibiting umami, bitter and sweet taste transduction.

Taste Map

Neural Pathways

First order neurons - From the receptors located in the taste buds, gustatory nerve afferents project to the ipsilateral rostral 1/3 of the nucleus tractus solitarius (NTS), located in the medulla. This rostral 1/3 is commonly referred to as the gustatory nucleus.

The gustatory nucleus receives input from cranial nerves VII (Facial n.), IX (Hypglossal n.), and X (Vagus n.) via special visceral afferent (SVA) nerve fibres. A summary of their functions is as follows:

  • Facial nerve n. - carries taste information from the anterior 2/3 of the tongue.
  • Hypoglossal n. - carries taste information from the posterior 1/3 of the tongue.
  • Vagus n. - carries taste information from the palate


Second order neurons - From the NTS second order neurons carry taste information to the Ventral posteromedial (VPM) nucleus in the thalamus.

Copious scientific conjecture surrounds how each taste modality is transmitted to the brain. The ‘labelled-line’ hypothesis suggests that there are taste specific neurons which exclusively carry that taste modality to cortical areas. In analogous terms it can be viewed like the pipelines leading toward a house. Each pipeline carries its own utility, there is one for gas, another for water and finally for electricity with each terminating in slightly different areas of the house. In the same way, on the tongues there are different receptors for each taste which concurrently have individual nerve tracts leading to the primary gustatory cortex.

Contrapuntally there is electrophysiological evidence that single nerve afferents carry multiple modalities. These studies show one nerve afferent may have both a strong and a weak activation in response to a multiple taste stimuli.

Cortical Areas

Primary gustatory cortex

The location of the taste perception centres has been observed via functional magnetic resonance imaging (fMRI) studies. The primary taste cortex has been identified as being located in the anterior insula/frontal operculum (I/fO), with the secondary taste cortex in the caudolateral orbitofrontal cortex. Within the insula there are further subdivisions related to each taste modality. Two photon calcium imaging research has outlined certain ’hot-spots’ of activation which are clearly delineated in relation to each taste. For example, the bitter modality is represented on the insula cortex approximately 1mm posterior to the middle cerebral artery whereas the sweet modality is represented 2.5mm rostrodorsal to the bitter field with no apparent overlap. These findings lend weight to the idea that there is only one receptor for each taste quality.


It is interesting to note the relationship between taste and the reward centres of the brain. There is evidence of neural input to the ventral tegmental area (the origin of the mesolimbic dopaminergic reward pathway) and nucleus accumbens (involved in the conversion of motivation into physical action). Neural connections such as this provide the foundations in explaining concepts such as flavour learning and preference. It may also give some insight into chronic problems such as food addiction and craving. As of yet however the gustatory neural network is not well understood and mor research is required to elucidate the relationship between pleasure and taste sensation

Timeline of Developmental Processes of the Gustatory System

Postovulatory Week Description Image
Week 6

The surface of the developing tongue is covered by nearly flat epitheliuem (Figure 1) [1]. The first gustatory papillae of the tongue appears in the caudal midline near the foramen caecum [1] . On the dorsal midline the first circumvallate papilla develops (Figure 2) [1]

image

Weeks 6 to 7

Nerve fibres approach the basal lamina of lingual epithelium [2]. At this stage the lingual epithelium consists of two to three cell layers and there is not yet any sign of cell specializations indicating early taste bud formation[2].

Image

Week 7

A series of epithelial swellings in the anterior part and midline of the tongue indicate early forming fungiform papillae (figure 2) [1].

Image

Week 8

The lingual epithelium shows first signs of taste bud development as nerve fibres penetrate epithelial basal lamina [2] and form synapses with taste bud progenitor cells. The synapses reach a maximum around the 12th to 13th week [2]. However, at this time these cells are still poorly differentiated, elongated epithelial cells [2] . The synapses demonstrate the neuronal connection between the taste bud primordium and the central nervous system [2]. Ciliated cells also appear around the 8th week, however the significance of these cells which are scattered randomly across the lingual surface remains unclear [2].

Image

Weeks 8-9

“By this stage the top surface of circumvallate papillae usually contains a taste pit partly filled with microvilli of presumed underlying taste bud cells” (Figure 10) [1]

Image

Weeks 10-11

At this stage, the lingual epithelium compromises of about four cell layers, and the first shallow grooves above the taste bud primordium are developed [2].

Fungiform papillae appear on the lateral margins and the tip of the tongue [1], containing taste bud primordial that display the first signs of a primitive pore formation [2].

Untypically differentiated apical cellular processes extend onto the surface [1]

Some taste bud primordial contain cells that perforate the covering epithelial later with short, broad untypically differentiated apical cellular processes. (Figure 3,4) [2] [1]

Image

Week 12

Taste bud cells are more clearly differentiated into epithelial cell types II and III [2].

Image

Weeks 12-13

By this stage the taste bud primordial are all located on the top of dermal papillae (Fig 6a) [2]. There is also maximum synapses between cells and afferent nerve fibres, which intermingle with each other to form a plexus-like structure [2].

Image

Weeks 14 -15

The shape of the taste buds primordial begins to resemble those of adult taste buds [2]. By the 14th week the taste pores develop as the taste pits are filled by microvilli [2], indicating the possibility that the taste buds begin their gustatory function [2], however they still lack an electron-dense mucous material (Fig.10) [2]. The tastebuds only achieve a fully developed function in week 15 of gestation with the development of type I cells to produce the mucous material in the taste pit [2].

Image


History of Discoveries

Date Significant Discovery
350BC Aristotle writes about the basic tastes, sweet and bitter, which can be modified, he says, by salty and acidic.
1901 D. Hanig publishes a paper containing data of taste sensitivity in different regions of the tongue. The data are later misinterpreted, giving rise to the myth of the ‘tongue map’
1908 Fifth basic taste discovered: savouriness, described as umami, which is conferred by glutamate.
1931 Bitter taste sensitivity found to vary among humans (1)
1931-32 Genetecists confirm findings about sensitivity to bitter tasting PTC and discover non-tasting is a recessive genetic trait (2,11)
1939 Geneticists show that chimpansees like humans, vary in their ability to perceive the bitterness of PTC
1992 Discovery of gustucin, a teste cell-specific G-protein, in the taste buds. Gustucin is later shown to mark bitter, umami and sweet cells (13)
1992 Discovery of gustucin, a teste cell-specific G-protein, in the taste buds. Gustucin is later shown to mark bitter, umami and sweet cells (13)
1996 Witt M and Reutter K of the Source Department of Anatomy, Technical University Dresden, Germany, carried out a transmission electron microscopy study to investigate the embryonic and fetal development of Human taste buds. Their results suggest an "at least dual function of embryonic/fetal taste buds", including non-gustatory, paracrine functions prior to the 14th week and gustatory after the 14th week. PMID 8955790
2000 First taste sonsors, the T2R receptors, discovered (3)
2001 The sweet receptor is discovered (5): a combination of TaR2 and T1R3.
2002 Amino acid detector, T1R1 and T1R3 identified (6) = Umami
2005 Sweet taste receptor found (15) in the GI tract
2006 Cells for sour taste discovered, identified by PKD2L1 (4-7)
2007 Harlow DE, Barlow LA of the Department of Cell and Developmental Biology, Rocky Mountain Taste and Smell Center, University of Colorado Denver Health Sciences Center provide evidence of the embryonic origin of gustatory cranial sensory neurons PMID 17826760
2009 The Car4 receptor, which senses the carbon dioxide in fizzy drinks is found on sour cells (8)


A study by Hevezi P et al, presents the first comprehensive characterization of gene expression in primate taste buds, as opposed to previous studies which focused on rodents. PMID 19636377

2010 ENaC identified as the sodium-salt taste receptor (9)


Adult Tongue and Taste Buds – Structure and Function

The tongue is for tasting, swallowing, and speech.The tongue is located on the floor of the oral cavity, It is a muscular structure with sensory units crowning. The tongue is divided into an anterior two thirds and a posterior one third. These regions are divided by a V-shaped groove at the back of the tongue (sulcus terminalis). The anterior two thirds of the tongue is covered by stratified squamous epithelium, It contains a roughened surface and has projections called papillae that vary in shape and number. The most numerous papillae are the filiform papillae, which function to provide a surface that aids in holding food on the tongue during chewing. The larger, less numerous fungiform papillae are scattered among the filiform papillae where as the Circumvallate papilae form a wide V at the sulcus terminalis. There are no papillae or taste buds located on the posterior third of the tongue, having mucosal folds and the lingual tonsils instead.

The Image Below is a very simplistic Hand drawn Diagram of the surface of the Tongue showing the locations of the different papillae & and also the sulcus terminalis, This image indicates the tongue from an above veiw


Drawing of Tongue.png

The functional unit of the taste bud is a taste cells, there are between 50 and 100 taste cells in each taste bud these taste cells represent all 5 different tastes. Historically is was believed that different areas of the tongue were responsible for different taste sensations although this has since been disregarded.[1] The papillae contain taste buds which are connected to the oral cavity via a taste pore, the function of the taste bud is to transmit a chemical signal from the oral cavity to a taste cell, this chemical signal is the converted into an electrical impulse and delivered to the brain via nerve fibres for interpretation.


The Image Below is a very simplistic Hand drawn Diagram of a taste bud, in an extreme close up, cross section view of the the taste bud unit, not to scale


Taste bud 1.jpg


Abnormalities

(try to include technologies to detect abnormalities during pregnancy)

Knocking out P2X Receptors

Gustatory abnormalities has not been widely researched and what has been researched has been through animal testing, most commonly we have found on mice.

In Huang, 2008 [PMID:21940456] this team used the release of the neurotransmitter, ATP (adenosine triphosphate) as a quantitative measurement of gustatory sensation and taste. This was done by using a comparison of wild type (WT) and double knockout (DKO) mice. P2X receptors, P2X2 and P2X3 were knocked out in the DKO mice. The premise of this article was that knocking out P2X receptors reduces transmitter secretion of ATP in taste buds, therefore they cannot taste.

It should be noted that the taste buds in DKO are functional, but are not stimulated by the administration of tastants.

The transmission of release of ATP is secretion through gap junction hemichannels (pannexin 1 gap junction).

When both P2X2 and P2X3 are knocked out, no taste is elicited. However they found that if either P2X2 OR P2X3 was knocked out there was a taste response. So the inference made from this is that if one of the two receptors from the P2X family was knocked out there still can have taste response. The WT mice showed significant stimulation by tastants whereas DKO had little to no stimulation of ATP release.

Insert image 4 from article here

FGF signalling and genes

Sprouty, or Srpy, genes have been related to regulating the development of circumvillate papillae (CVP). The CVP are large dome shaped papillae, which form a 'V' just in front of the terminal sulcus.

By knocking out Spry genes using mice which had Spry1 and Spry2 knocked out showed that the number of CVP doubled. However, when Fibroblast Growth Factor gene ("Fgf10") was absent, the number of CVP was significantly reduced, if not completely absent. The correlation between Spry1/2 and Fgf10 is that Spry1/2 antagonises "Fgf10" to limit the size of the CVP progenitor placode. Exclusive expression of Fgf10 in the mesenchyme is necessary for the formation of CVP.

This image shows the CVP stained with H&E and scanned by Scanning Electron Microscope (SEM) CVP of WT(top) and DKO(bottom) mice with H&E and SEM.png

Taste buds develop from the mesenchyme but require local signalling to properly differentiate. Some signalling factors for proper development of taste buds besides FGF are: Sonic Hedgehog (SHH), Bone Morphogenetic Proteins (BMPs), Epidermal Growth Factor (EGF).


Additionally, this proves that the anterior and posterior developments of the tongue are derived from embryonic tissues, where the anterior tongue is derived fromt he ectoderm and the posterior tongue is derived from the endoderm.

Current Research

We have an evolving understanding of Embryonic Taste development, through the use of state-of-the-art technology and research techniques we are able to make brilliant discoveries that continue to connect the dots of this amazing natural process of human development. The majority of research in taste development is involving mice, these mammals show similar embryonic pathways to humans and research is performed in ethical and humane methods.

the below image shows histological stains of a mice tongue

Histology.jpg

In an animal study using mice by Suzuki Y, Ikeda K, Kawakami K.(2011) Firstly nominating "Six Genes" as a major component in gustatory development, stating that deficiencies in certain Six genes (specifically Six1 & Six4) leads to poor development. Their research also highlights evidence of cooperative relationships between Six genes for normal advance. Understanding the role of certain genes along with the intrinsic relationships they hold is crucial for the ability to identify possible causes and correction of any abnormalities [7] Another Animal Study involving mice explores a new idea of Neural crest contribution in taste development, specifically the development of papillae and taste buds. Liu HX, Komatsu Y, Mishina Y, Mistretta CM. (2012) suggest that Neural crest cells travel to the location of the tongue in early embryonic stages, gain epithelium phenotypes, multiply and then differentiate to eventually form taste papillae. Appreciating embryonic courses allows constant monitoring throughout expansion of the foetus.[8] In an animal study conducted by Rothova M, Thompson H, Lickert H, Tucker AS.(2012) exploring the historically debated issue of endoderm contribution to tongue development showed promising evidence that position of taste buds are patterned by the border of ectoderm and endoderm derivative epithelium. [9] Research by Ozdener H, Spielman AI, Rawson NE.(2012) developing a culture which allows taste cells to survive for up to 12 months, empowers researchers to study the processes of proliferation, differentiation and function. [10] Brain-derived neurotrophic factor (BDNF) was identified to promote gustatory neuron development, along with identifying the embryonic precursor populations for cranial ganglia in mice through the use of fate mapping in a study by Harlow, Yang, Williams, Barlow (2011)[11] The WNT gene family has a function of signally proteins for various reason such as development, In 2010 Liu, Staubach Grosse, Walton, Saims, Gumucio, Mistretta explored recent findings on the role of WNT's in tongue and papillae development, Concluding that WNT/β-catenin is essential for fungiform papillae differing to WNT5a which proved to be principle in tongue development. [12]

The below image shows and example of tongue abnormalities, this is called "double tongue" each side has independent movement [2] Abnormal of tongue.jpg

Useful links

Glossary

Image gallery

References

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 <pubmed>9455607</pubmed>
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 <pubmed>8955790</pubmed>
  3. <pubmed>9455607</pubmed>
  4. <pubmed>9541477</pubmed>
  5. <pubmed>8241557</pubmed>
  6. <pubmed>22717400</pubmed>
  7. <pubmed>21978088</pubmed>
  8. <pubmed>22659543</pubmed>
  9. <pubmed>ref>22581563</pubmed>
  10. <pubmed>ref>22643728</pubmed>
  11. <pubmed>ref>PMC2674259</pubmed>
  12. <pubmed>ref>PMC2768563</pubmed>


Glossary

External Links

External Links Notice - The dynamic nature of the internet may mean that some of these listed links may no longer function. If the link no longer works search the web with the link text or name. Links to any external commercial sites are provided for information purposes only and should never be considered an endorsement. UNSW Embryology is provided as an educational resource with no clinical information or commercial affiliation.

--Mark Hill 12:22, 15 August 2012 (EST) Please leave the content listed below the line at the bottom of your project page.


2012 Projects: Vision | Somatosensory | Taste | Olfaction | Abnormal Vision | Hearing