2010 Lab 8: Difference between revisions

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<div style="background:#F5FFFA; border: 1px solid #CEF2E0; padding: 1em; margin: auto; width: 95%; float:left;"><div style="margin:0;background-color:#cef2e0;font-family:sans-serif;font-size:120%;font-weight:bold;border:1px solid #a3bfb1;text-align:left;color:#000;padding-left:0.4em;padding-top:0.2em;padding-bottom:0.2em;">Laboratory Notice - Mark Hill</div>This online lab page will contain the content required when attending the practical. Currently this page is only a template and will be updated before the practical (this notice removed when completed).</div>
==Introduction==
This laboratory will study the early development of both the Renal and Genital systems, often grouped as the Urogenital System.
 
'''Mid-Embryonic (stage 13)'''
* Renal middle stage (mesonephros) and septating cloacal region.
* Gonad is present only as indifferent genital ridge stage.
 
'''Late Embryonic (stage 22)'''
* Renal kidney developing (metanephros) and degenerating middle stage (mesonephros).
* Gonad is differentiating and the internal genital tract (mesonephric/paramesonephric duct) is differentiating.
 
'''Early Fetal'''
* Renal kidney has identifiable cortex and medulla and is retroperitoneal. Note the relative size of the kidney and adrenal gland.
* Gonad is now different for male and female and internal genital tract is developing. External genitalia is indifferent.
 
 
 
'''Links:''' [http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=mboc4.figgrp.3716 Influence of Sry on gonad development] | [http://www.ncbi.nlm.nih.gov/bookshelf/br.fcgi?book=endocrin&part=A972&rendertype=box&id=A1230 Comparative anatomy of adult male and female reproductive tracts]


==Stage 13==
==Stage 13==
Line 69: Line 86:
| [[:File:Stage 13 image 098.jpg|G7L]]
| [[:File:Stage 13 image 098.jpg|G7L]]
|}
|}
==Introduction==
{| border='0px'
|-
| [[File:Stage22-UG-icon.jpg|160px|link=Movie_-_Urogenital_System_3D_stage_22]]
| [[Movies_-_Embryo_Carnegie_stage_22|Movies - Embryo Carnegie stage 22]] - This embryo animation rotates and show the relative position of internal renal system structures at the end of embryonic development. Compare these with the earlier [[Renal System - Carnegie Stage 13|stage 13]] embryo.
The movies are based upon reconstruction of serial slice images.
|-
| [[Movie_-_Urogenital_System_3D_stage_22|Urogenital]]
|
|-
|}
:{{Template:Renal Links}}
== Stage 22 Renal Annotated ==
{| class="prettytable"
|[[File:Stage_22 image 083.jpg|160px]] E6: R,L adrenal glands under diaphragm.
[[File:Stage_22 image 084.jpg|160px]] E7: Large adrenal glands. Inferior vena cava. Thoracic aorta.
| [[File:Stage_22 image 085.jpg|160px]]
| [[File:Stage_22 image 086.jpg|160px]]
| [[File:Stage_22 image 087.jpg|160px]]
|-
|
| Fl: Adrenal glands. R. Kidney. Autonomic ganglia (partly the adrenal medulla precursors).
| F2: Kidneys (note retroperitoneal location). Cortex. Medulla. L. Adrenal gland. Superior mesenteric artery. Inferior vena cava.
| F3: R testis (note its location relative to the R adrenal). L adrenal. R renal hilus. large channels are branches of ureteric tree.
|-
| [[File:Stage_22 image 088.jpg|160px]]
| [[File:Stage_22 image 089.jpg|160px]]
| [[File:Stage_22 image 090.jpg|160px]]
| [[File:Stage_22 image 091.jpg|160px]]
|-
| F4: R kidney and R ureter. Inferior vena cava. L. kidney, L renal hilus and L ureter. R testis with R mesonephric duct (precursor of vas deferens). L testis. Umbilical arteries passing into umbilical cord allantois between them.
| F5: Kidneys. Ureters. Note umbilical arteries and allantois. Also note how R testis and mesonephric structures are attached to parietal peritoneum by a mesogonad.
| F6: Kidneys. Ureters. Note umbilical arteries and allantois. Also note how R testis and mesonephric structures are attached to parietal peritoneum by a mesogonad.
| F7: In F7, (dorsal to R testis and liver) note with the distinct lumen of the mesonephric duct, almost solid column of paramesonephric cells and remnants of mesonephric tubules. "mesogonad". Ureters. Bladder with submucosa and detrusor muscle. Umbilical arteries. Division of aorta.
|-
| [[File:Stage_22 image 092.jpg|160px]]
| [[File:Stage_22 image 093.jpg|160px]]
| [[File:Stage_22 image 094.jpg|160px]]
| [[File:Stage_22 image 095.jpg|160px]]
|-
| G1: Ureters, Bladder. Umbilical arteries. Testis with remains of mesonephros (dorsal), mesonephric duct and paramesonephric cells. Sigmoid colon and mesocolon.
| G2: Ureters being displaced ventrally, crossing common iliac arteries. Sigmoid colon. Bladder. Mesonephric ducts (lateral) and paramesonephric ducts (smaller, medial) located dorsal to bladder.
| G3: Ureters (cut twice): descending dorsal to bladder and ascending ventrally to enter the bladder at trigone, through the submucosa). Fusion of paramesonephric ducts. Paired mesonephric ducts. Umbilical arteries looping off common iliac arteries. Pubic symphysis. Colon.
| G4: Most caudal part of loop of ureters. Urethra emerging from bladder. Mesonephric ducts. Rectocolic junction.
|-
| [[File:Stage_22 image 096.jpg|160px]]
| [[File:Stage_22 image 097.jpg|160px]]
| [[File:Stage_22 image 098.jpg|160px]]
|
|-
| G5: Urethra (in region of future prostate gland - note crescentic shape). Rectum. Rectovesical pouch. Between G4 and G5, each mesonephric duct (vas deferens) has joined the prostatic urethra (caudal to the ureters), thereby increasing the caliber of the latter.
| G6: Penile urethra, emerging inferiorly to the glans penis. Scrotal swellings (appear before testis descends).
| G7: Penile urethra, emerging inferiorly to the glans penis. Scrotal swellings (appear before testis descends).
| '''Note F7 MS term:''' ''"inebriated Puffin" ''(dorsal to R testis and liver) lumen of the mesonephric duct (eye), almost solid column of paramesonephric cells (beak) and remnants of mesonephric tubules (body).
|}
&nbsp;


==Stage 22==
==Stage 22==
Line 108: Line 201:
| [[:File:Stage_22 image 098.jpg|G7L]]
| [[:File:Stage_22 image 098.jpg|G7L]]
|}
|}
==Stage 22 Selected (male)==
{|
|+'''[[Carnegie_stage_22_-_selected_serial_sections|Stage 22]]'''  ''selected serial labeled images''
|-
|
|
|
| [[File:Stage 22 image 188.jpg|120px]]
| [[File:Stage 22 image 189.jpg|120px]]
| [[File:Stage 22 image 190.jpg|120px]]
| [[File:Stage 22 image 191.jpg|120px]]
|-
|
|
|
| [[:File:Stage 22 image 188.jpg|F4 kidney]]
| [[:File:Stage 22 image 189.jpg|F5 renal pelvis]]
| [[:File:Stage 22 image 190.jpg|F6 nephron]]
| [[:File:Stage 22 image 191.jpg|F7 gonad]]
|-
| [[File:Stage 22 image 192.jpg|120px]]
| [[File:Stage 22 image 193.jpg|120px]]
| [[File:Stage 22 image 194.jpg|120px]]
| [[File:Stage 22 image 195.jpg|120px]]
| [[File:Stage 22 image 196.jpg|120px]]
| [[File:Stage 22 image 197.jpg|120px]]
| [[File:Stage 22 image 198.jpg|120px]]
|-
| [[:File:Stage 22 image 192.jpg|G1 ureter]]
| [[:File:Stage 22 image 193.jpg|G2 mesonephros]]
| [[:File:Stage 22 image 194.jpg|G3 testis]]
| [[:File:Stage 22 image 195.jpg|G4 rete tesits]]
| [[:File:Stage 22 image 196.jpg|G5 urogenital]]
| [[:File:Stage 22 image 197.jpg|G6 urogenital]]
| [[:File:Stage 22 image 198.jpg|G7 urogenital]]
|}
==Stage 22 Selected==
{|
|+'''[[Carnegie_stage_22_-_selected_serial_sections|Stage 22]]'''  ''selected serial labeled images''
|-
|
|
|
| [[File:Stage 22 image 188.jpg|120px]]
| [[File:Stage 22 image 189.jpg|120px]]
| [[File:Stage 22 image 190.jpg|120px]]
| [[File:Stage 22 image 191.jpg|120px]]
|-
|
|
|
| [[:File:Stage 22 image 188.jpg|F4 kidney]]
| [[:File:Stage 22 image 189.jpg|F5 renal pelvis]]
| [[:File:Stage 22 image 190.jpg|F6 nephron]]
| [[:File:Stage 22 image 191.jpg|F7 gonad]]
|-
| [[File:Stage 22 image 192.jpg|120px]]
| [[File:Stage 22 image 193.jpg|120px]]
| [[File:Stage 22 image 194.jpg|120px]]
| [[File:Stage 22 image 195.jpg|120px]]
| [[File:Stage 22 image 196.jpg|120px]]
| [[File:Stage 22 image 197.jpg|120px]]
| [[File:Stage 22 image 198.jpg|120px]]
|-
| [[:File:Stage 22 image 192.jpg|G1 ureter]]
| [[:File:Stage 22 image 193.jpg|G2 mesonephros]]
| [[:File:Stage 22 image 194.jpg|G3 testis]]
| [[:File:Stage 22 image 195.jpg|G4 rete tesits]]
| [[:File:Stage 22 image 196.jpg|G5 urogenital]]
| [[:File:Stage 22 image 197.jpg|G6 urogenital]]
| [[:File:Stage 22 image 198.jpg|G7 urogenital]]
|}
==Fetal 10 Week (female)==
{|
| <center>'''Lateral'''</center>
| <center>'''Midline'''</center>
|-
| [[File:Fetal 10wk urogenital 1.jpg|400px|most lateral]]
| [[File:Fetal 10wk urogenital 3.jpg|400px|medial]]
|-
| [[File:Fetal 10wk urogenital 2.jpg|400px|lateral]]
| [[File:Fetal 10wk urogenital 4.jpg|400px|midline]]
|}
'''Urogenital Labelled''' -  [[:File:Fetal 10wk urogenital 1.jpg|most lateral]]  | [[:File:Fetal 10wk urogenital 2.jpg|lateral]] | [[:File:Fetal 10wk urogenital 3.jpg|medial]] | [[:File:Fetal 10wk urogenital 4.jpg|midline]]
==Urogenital Movies==
{| border='0px'
|-
| [[File:Urogenital_sinus_001 icon.jpg|90px|link=Development_Animation_-_Urogenital_Sinus]]
| [[File:Urogenital_septum_001 icon.jpg|90px|link=Development_Animation_-_Urogenital_Septum]]
| [[File:Trigone_001_icon.jpg|90px|link=Development Animation - Trigone]]
| [[File:Renal_001_icon.jpg|90px|link=Development Animation - Renal]]
|-
| [[Development_Animation_-_Urogenital_Sinus|Urogenital Sinus]]
| [[Development_Animation_-_Urogenital_Septum|Urogenital Septum]]
| [[Development Animation - Trigone|Trigone]]
| [[Development Animation - Renal|Renal Nephron]]
|-
| [[File:Gonad-icon.jpg|90px|link=Development Animation - Ovary‎‎]]
| [[File:Gonad-icon.jpg|90px|link=Development Animation - Testis‎‎]]
| [[File:Female_external_001 icon.jpg|90px|link=Development_Animation_-_Genital_Female_External]]
| [[File:Male_external_001 icon.jpg|90px|link=Development_Animation_-_Genital_Male_External]]
| [[File:Uterus_001 icon.jpg|90px|link=Development_Animation_-_Uterus]]
| [[File:Testis_001 icon.jpg|90px|link=Development_Animation_-_Testis_Descent]]
|-
| [[Development Animation - Ovary|‎‎Ovary]]
| [[Development Animation - Testis|Testis‎‎]]
| [[Development_Animation_-_Genital_Female_External|Female External]]
| [[Development_Animation_-_Genital_Male_External|Male External]]
| [[Development_Animation_-_Uterus|Uterus]]
| [[Development_Animation_-_Testis_Descent|Testis Descent]]
|-
|}
{| border='0px'
|+ '''Primordial germ cell migration in the mouse'''
|-
| [[File:Primordial germ cell 001 icon.jpg|90px|link=Movie_-_Primordial germ cell migration 01]]
| [[File:Primordial germ cell 002 icon.jpg|90px|link=Movie_-_Primordial germ cell migration 02]]
| [[File:Primordial germ cell 003 icon.jpg|90px|link=Movie_-_Primordial germ cell migration 03]]
|-
| [[Movie_-_Primordial germ cell migration 01|Migration 1]]
| [[Movie_-_Primordial germ cell migration 02|Migration 2]]
| [[Movie_-_Primordial germ cell migration 03|Migration 3]]
|-
|}
==Group Project==
--[[User:S8600021|Mark Hill]] 15:01, 22 September 2010 (UTC) ''Any student who has not now added an assessment to every project discussion page and included a copy of all assessments given on their own page will a receive '''zero mark''' for this individual assessment item.''
Group Projects (20% of your final mark). It is now week 9 and the projects should now have been peer assessed by your class. It is now time for your group to review the comments and constructive criticisms that have been added to your project discussion page.
'''Begin''' by collating the comments.
* What are the common criticisms?
* What were the best aspects identified within your project?
* What errors, typos, missing references were identified?
* Were there contributions from individual group members that were identified as good or poor parts of the overall project?
'''Then''' work on the changes.
* Develop priorities.
* Divide the changes and corrections between group members.
* Are there additional changes that should be made that were not identified by peer assessment.
You now have 2 weeks to complete all project work before final assessment.
{{Template:2010ANAT2341}}
[[Category:2010ANAT2341]]
[[Category:Science-Undergraduate]]
[[Category:Renal]] [[Category:Genital]]

Latest revision as of 02:10, 23 September 2010

Introduction

This laboratory will study the early development of both the Renal and Genital systems, often grouped as the Urogenital System.

Mid-Embryonic (stage 13)

  • Renal middle stage (mesonephros) and septating cloacal region.
  • Gonad is present only as indifferent genital ridge stage.

Late Embryonic (stage 22)

  • Renal kidney developing (metanephros) and degenerating middle stage (mesonephros).
  • Gonad is differentiating and the internal genital tract (mesonephric/paramesonephric duct) is differentiating.

Early Fetal

  • Renal kidney has identifiable cortex and medulla and is retroperitoneal. Note the relative size of the kidney and adrenal gland.
  • Gonad is now different for male and female and internal genital tract is developing. External genitalia is indifferent.


Links: Influence of Sry on gonad development | Comparative anatomy of adult male and female reproductive tracts

Stage 13

Stage 13 serial labeled images
Stage 13 image 077.jpg
Stage 13 image 078.jpg Stage 13 image 079.jpg Stage 13 image 080.jpg Stage 13 image 081.jpg Stage 13 image 082.jpg Stage 13 image 083.jpg Stage 13 image 084.jpg
E1L E2L E3L E4L E5L E6L E7L
Stage 13 image 085.jpg Stage 13 image 086.jpg Stage 13 image 087.jpg Stage 13 image 088.jpg Stage 13 image 089.jpg Stage 13 image 090.jpg Stage 13 image 091.jpg
F1L F2L F3L F4L F5L F6L F7L
Stage 13 image 092.jpg Stage 13 image 093.jpg Stage 13 image 094.jpg Stage 13 image 095.jpg Stage 13 image 096.jpg Stage 13 image 097.jpg Stage 13 image 098.jpg
G1L G2L G3L G4L G5L G6L G7L


Introduction

Stage22-UG-icon.jpg Movies - Embryo Carnegie stage 22 - This embryo animation rotates and show the relative position of internal renal system structures at the end of embryonic development. Compare these with the earlier stage 13 embryo.

The movies are based upon reconstruction of serial slice images.

Urogenital


Renal Links: renal | Lecture - Renal | Lecture Movie | urinary bladder | Stage 13 | Stage 22 | Fetal | Renal Movies | Stage 22 Movie | renal histology | renal abnormalities | Molecular | Category:Renal
Historic Embryology - Renal  
1905 Uriniferous Tubule Development | 1907 Urogenital images | 1911 Cloaca | 1921 Urogenital Development | 1915 Renal Artery | 1917 Urogenital System | 1925 Horseshoe Kidney | 1926 Embryo 22 Somites | 1930 Mesonephros 10 to 12 weeks | 1931 Horseshoe Kidney | 1932 Renal Absence | 1939 Ureteric Bud Agenesis | 1943 Renal Position

Stage 22 Renal Annotated

Stage 22 image 083.jpg E6: R,L adrenal glands under diaphragm.

Stage 22 image 084.jpg E7: Large adrenal glands. Inferior vena cava. Thoracic aorta.

Stage 22 image 085.jpg Stage 22 image 086.jpg Stage 22 image 087.jpg
Fl: Adrenal glands. R. Kidney. Autonomic ganglia (partly the adrenal medulla precursors). F2: Kidneys (note retroperitoneal location). Cortex. Medulla. L. Adrenal gland. Superior mesenteric artery. Inferior vena cava. F3: R testis (note its location relative to the R adrenal). L adrenal. R renal hilus. large channels are branches of ureteric tree.
Stage 22 image 088.jpg Stage 22 image 089.jpg Stage 22 image 090.jpg Stage 22 image 091.jpg
F4: R kidney and R ureter. Inferior vena cava. L. kidney, L renal hilus and L ureter. R testis with R mesonephric duct (precursor of vas deferens). L testis. Umbilical arteries passing into umbilical cord allantois between them. F5: Kidneys. Ureters. Note umbilical arteries and allantois. Also note how R testis and mesonephric structures are attached to parietal peritoneum by a mesogonad. F6: Kidneys. Ureters. Note umbilical arteries and allantois. Also note how R testis and mesonephric structures are attached to parietal peritoneum by a mesogonad. F7: In F7, (dorsal to R testis and liver) note with the distinct lumen of the mesonephric duct, almost solid column of paramesonephric cells and remnants of mesonephric tubules. "mesogonad". Ureters. Bladder with submucosa and detrusor muscle. Umbilical arteries. Division of aorta.
Stage 22 image 092.jpg Stage 22 image 093.jpg Stage 22 image 094.jpg Stage 22 image 095.jpg
G1: Ureters, Bladder. Umbilical arteries. Testis with remains of mesonephros (dorsal), mesonephric duct and paramesonephric cells. Sigmoid colon and mesocolon. G2: Ureters being displaced ventrally, crossing common iliac arteries. Sigmoid colon. Bladder. Mesonephric ducts (lateral) and paramesonephric ducts (smaller, medial) located dorsal to bladder. G3: Ureters (cut twice): descending dorsal to bladder and ascending ventrally to enter the bladder at trigone, through the submucosa). Fusion of paramesonephric ducts. Paired mesonephric ducts. Umbilical arteries looping off common iliac arteries. Pubic symphysis. Colon. G4: Most caudal part of loop of ureters. Urethra emerging from bladder. Mesonephric ducts. Rectocolic junction.
Stage 22 image 096.jpg Stage 22 image 097.jpg Stage 22 image 098.jpg
G5: Urethra (in region of future prostate gland - note crescentic shape). Rectum. Rectovesical pouch. Between G4 and G5, each mesonephric duct (vas deferens) has joined the prostatic urethra (caudal to the ureters), thereby increasing the caliber of the latter. G6: Penile urethra, emerging inferiorly to the glans penis. Scrotal swellings (appear before testis descends). G7: Penile urethra, emerging inferiorly to the glans penis. Scrotal swellings (appear before testis descends). Note F7 MS term: "inebriated Puffin" (dorsal to R testis and liver) lumen of the mesonephric duct (eye), almost solid column of paramesonephric cells (beak) and remnants of mesonephric tubules (body).

 

Stage 22

Stage 22L serial labeled images
Stage 22 image 085.jpg Stage 22 image 086.jpg Stage 22 image 087.jpg Stage 22 image 088.jpg Stage 22 image 089.jpg Stage 22 image 090.jpg Stage 22 image 091.jpg
F1L F2L F3L F4L F5L F6L F7L
Stage 22 image 092.jpg Stage 22 image 093.jpg Stage 22 image 094.jpg Stage 22 image 095.jpg Stage 22 image 096.jpg Stage 22 image 097.jpg Stage 22 image 098.jpg
G1L G2L G3L G4L G5L G6L G7L

Stage 22 Selected (male)

Stage 22 selected serial labeled images
Stage 22 image 188.jpg Stage 22 image 189.jpg Stage 22 image 190.jpg Stage 22 image 191.jpg
F4 kidney F5 renal pelvis F6 nephron F7 gonad
Stage 22 image 192.jpg Stage 22 image 193.jpg Stage 22 image 194.jpg Stage 22 image 195.jpg Stage 22 image 196.jpg Stage 22 image 197.jpg Stage 22 image 198.jpg
G1 ureter G2 mesonephros G3 testis G4 rete tesits G5 urogenital G6 urogenital G7 urogenital

Stage 22 Selected

Stage 22 selected serial labeled images
Stage 22 image 188.jpg Stage 22 image 189.jpg Stage 22 image 190.jpg Stage 22 image 191.jpg
F4 kidney F5 renal pelvis F6 nephron F7 gonad
Stage 22 image 192.jpg Stage 22 image 193.jpg Stage 22 image 194.jpg Stage 22 image 195.jpg Stage 22 image 196.jpg Stage 22 image 197.jpg Stage 22 image 198.jpg
G1 ureter G2 mesonephros G3 testis G4 rete tesits G5 urogenital G6 urogenital G7 urogenital


Fetal 10 Week (female)

Lateral
Midline
most lateral medial
lateral midline


Urogenital Labelled - most lateral | lateral | medial | midline

Urogenital Movies

Urogenital sinus 001 icon.jpg Urogenital septum 001 icon.jpg Trigone 001 icon.jpg Renal 001 icon.jpg
Urogenital Sinus Urogenital Septum Trigone Renal Nephron
Gonad-icon.jpg Gonad-icon.jpg Female external 001 icon.jpg Male external 001 icon.jpg Uterus 001 icon.jpg Testis 001 icon.jpg
‎‎Ovary Testis‎‎ Female External Male External Uterus Testis Descent
Primordial germ cell migration in the mouse
Primordial germ cell 001 icon.jpg Primordial germ cell 002 icon.jpg Primordial germ cell 003 icon.jpg
Migration 1 Migration 2 Migration 3

Group Project

--Mark Hill 15:01, 22 September 2010 (UTC) Any student who has not now added an assessment to every project discussion page and included a copy of all assessments given on their own page will a receive zero mark for this individual assessment item.

Group Projects (20% of your final mark). It is now week 9 and the projects should now have been peer assessed by your class. It is now time for your group to review the comments and constructive criticisms that have been added to your project discussion page.

Begin by collating the comments.

  • What are the common criticisms?
  • What were the best aspects identified within your project?
  • What errors, typos, missing references were identified?
  • Were there contributions from individual group members that were identified as good or poor parts of the overall project?

Then work on the changes.

  • Develop priorities.
  • Divide the changes and corrections between group members.
  • Are there additional changes that should be made that were not identified by peer assessment.


You now have 2 weeks to complete all project work before final assessment.


Glossary Links

Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | Numbers | Symbols | Term Link

Course Content 2010

Embryology Introduction | Cell Division/Fertilization | Lab 1 | Week 1&2 Development | Week 3 Development | Lab 2 | Mesoderm Development | Ectoderm, Early Neural, Neural Crest | Lab 3 | Early Vascular Development | Placenta | Lab 4 | Endoderm, Early Gastrointestinal | Respiratory Development | Lab 5 | Head Development | Neural Crest Development | Lab 6 | Musculoskeletal Development | Limb Development | Lab 7 | Kidney | Genital | Lab 8 | Sensory | Stem Cells | Stem Cells | Endocrine | Lab 10 | Late Vascular Development | Integumentary | Lab 11 | Birth, Postnatal | Revision | Lab 12 | Lecture Audio | Course Timetable


Cite this page: Hill, M.A. (2024, March 28) Embryology 2010 Lab 8. Retrieved from https://embryology.med.unsw.edu.au/embryology/index.php/2010_Lab_8

What Links Here?
© Dr Mark Hill 2024, UNSW Embryology ISBN: 978 0 7334 2609 4 - UNSW CRICOS Provider Code No. 00098G