View source for 2010 BGD Lecture - Development of the Embryo/Fetus 2

#REDIRECT [[BGDA Lecture - Development of the Embryo/Fetus 2‏‎]]
==Introduction==

Activity: Lecture Title: Development of the embryo/fetus 2 Scheduled: 9am to 10am Tuesday 11 May 2010 in CLB7

This lecture covers the period of Embryonic development, in Humans from week 3 to week 8 and is divided into 23 Carnegie stages of embryonic development. There will also be a brief introduction to fetal development.

This period of development will also be covered in your Practical, which is currently being updated for 2010 and will be available online. Note, the period from week 9 to week 38 is considered Fetal development and will be covered in detail in the Laboratory 11.

* Embryonic Development
* Organ and System formation (Functioning / Not Functional)
* Dynamic changes internal and external
* Carnegie stages illustrate external development


'''Lecture latest version:''' [[BGDA_Lecture_-_Development_of_the_Embryo/Fetus_2|2013 Lecture]]

==Lecture Audio==
{| border='0px'

|-
| [[File:Mark_Hill.jpg|60px|left]]
| 

BGD Cycle A 2010 Audio - Dr Mark Hill Tuesday 11th May 2010 9-10 am CLB7  (This recording is not the UNSW iLecture, available elsewhere).

* [[2010_BGD_Lecture_-_Development_of_the_Embryo/Fetus_2|lecture page]]

* [[Media:BGD2010-EmbryoLecture02.mp3|listen]]

* [[:File:BGD2010-EmbryoLecture02.mp3|download]] (6.6 Mb MP3 56:37)
|}

==First 8 Weeks==
[[File:Human_Carnegie_stage_1-23.jpg]]

==Week 3==
'''Mesoderm''' means the "middle layer" and it is from this layer that nearly all the bodies connective tissues are derived. In early mesoderm development a number of transient structures will form and then be lost as  tissue structure is patterned and organised. Humans are vertebrates, with a "backbone", and the first  mesoderm structure we will see form after the notochord will be '''[[S#somite|somites]]'''.

Facts: Week 4, 22 - 23 days, 2 - 3.5 mm, Somite Number 4 - 12

View: This is a dorsal view of the human embryo, the amniotic membrane has been removed. Top embryo is an early stage 10, bottom is late stage 10.

<gallery>
File:Stage10 bf4b.jpg|Early stage 10
File:Stage10 bf5b.jpg|Late stage 10
File:Stage10 bf6b.jpg|Labeled stage 10
</gallery>

<gallery>
Image:Mesoderm cartoon1.gif|trilaminar embryo
Image:Mesoderm cartoon2.gif|mesoderm regions
Image:Mesoderm cartoon3.gif|somite coelom
Image:Mesoderm cartoon4.gif|neural tube and neural crest
</gallery>

'''Mesoderm organization:''' lateral plate - intermediate mesoderm - paraxial mesoderm - axial mesoderm - paraxial mesoderm - intermediate mesoderm - lateral plate

<gallery>
Image:Stage7_paraxial-mesoderm.jpg|Stage 7 paraxial mesoderm
Image:Stage7_intermediate-mesoderm.jpg|Stage 7 intermediate mesoderm
Image:Stage7_lateral-plate.jpg|Stage 7 lateral plate
</gallery>

===Axial Mesoderm===
* notochord
# mechanical role in embryonic disc folding
# molecular role in patterning surrounding tissues

<gallery>
Image:Stage7_800x700px.jpg|Stage 7 embryonic disc
Image:Stage7_primitive-streak-node.jpg|Stage 7 primitive-streak-node
Image:Stage7_cloacal-oral-membranes.jpg|Stage 7 cloacal-oral-membranes
Image:Stage7 notochord.jpg|Stage 7 notochord
</gallery>

'''Adult''' - contributes to the nucleus pulposis of the intervertebral disc

===Paraxial Mesoderm===
[[Image:Stage7_paraxial-mesoderm.jpg|thumb|Stage 7 paraxial mesoderm]]
* differentiates rostro-caudally (head to tail)
* remains unsegmented in the head region.
* segments in the body region to form pairs of somites along the length of the embryo.

'''Adult''' - contributes vertebral column (vertebra and IVD), dermis of the skin, skeletal muscle of body and limbs

===Intermediate Mesoderm===
[[Image:Stage7_intermediate-mesoderm.jpg|thumb|Stage 7 intermediate mesoderm]]
* named by position (between paraxial and lateral plate)
* differentiates rostro-caudally  (head to tail)
* forms 3 sets of "kidneys" in sequence
# pronephros
# mesonephros
# metanephros

'''Adult''' - metanephros forms the kidney

===Lateral Plate Mesoderm===
[[Image:Stage7_lateral-plate.jpg|thumb|Stage 7 lateral plate]]
* a "horseshoe shaped" space forms in the middle
* somatic mesoderm - closest to ectoderm
* space - forms the 3 body cavities (pericardial, pleural, peritoneal)
* splanchnic mesoderm - closest to endoderm

'''Adult''' - body connective tissues, gastrointestinal tract (connective tissues, muscle, organs), heart

===Somite Development===

[[File:Stage11 sem100c.jpg|stage 11 Embryo]]

Somite initially forms 2 main components
* ventromedial- sclerotome forms vertebral body and intervertebral disc
* dorsolateral - dermomyotome forms dermis and skeletal muscle

<gallery>
Image:Somite cartoon1.png|paraxial mesoderm
Image:Somite cartoon2.png|early somite
Image:Somite cartoon3.png|sclerotome and dermomyotome
Image:Somite cartoon4.png|dermatome and myotome
Image:Somite cartoon5.png|epaxial and hypaxial muscles
</gallery>

===Sclerotome===
* sclerotome later becomes subdivided
** rostral and caudal halves separated laterally by von Ebner's fissure
* half somites contribute to a single vertebral level body
* other half intervertebral disc
* therefore final vertebral segmentation “shifts”

===Myotome===
* Body - epaxial and hypaxial muscles
* Limbs - flexor and extensor muscles

===Dermatome===
* connective tissue underlying epidermis
* begins as a dorsal thickening
* spreads throughout the body

[[File:Mesoderm 001 icon.jpg|160px|link=Development_Animation_-_Mesoderm]] [[File:Somite 001 icon.jpg|160px|link=Development_Animation_-_Somite_Musculoskeletal]]  [[File:Vertabra 003 icon.jpg|160px|link=Development_Animation_-_Vertebra]]

==Week 4==

===Heart===

[[Image:Stage7_lateral-plate.jpg|thumb|Stage 7 lateral plate]]
[[File:Heart Looping Sequence (SEMs).jpg|600px]]
[[File:Mouse_embryo_vascular.png|thumb|Mesoderm vascular development]]

* forms initially in splanchnic mesoderm of prechordal plate region - '''cardiogenic region''' 
** growth and folding of the embryo moves heart ventrallly and downward into anatomical position 
* week 3 begins as paired heart tubes that fuse to form single heart tube 
* begins to beat in Humans- day 22-23 
* heart tube connects to blood vessels forming in splanchnic and extraembryonic mesoderm 

'''Week 2-3''' pair of thin -walled tubes 

'''Week 3''' tubes fused, truncus arteriosus outflow, heart contracting 

'''Week 4''' heart tube continues to elongate, curving to form S shape 

'''Week 5''' Septation starts, atrial and ventricular 

Septation continues, atrial septa remains open, foramen ovale

===Neural===

[[File:Stage10 neural sm.jpg|thumb|Stage 10 Week 4, 22 - 23 days]]
[[File:Stage10_sem9.jpg|thumb|Stage 10 neural groove to tube]]
====Neural Plate====
[[Image:Neuralplate cartoon.png|right]]

[[File:Neuralplate_001 icon.jpg|120px|link=Development Animation - Neural Plate]]

* extends from buccopharyngeal membrane to primitive node 
* forms above notochord and paraxial mesoderm 
* neuroectodermal cells 
** broad brain plate 
** narrower spinal cord 
* 3 components form: floor plate, neural plate, neural crest 

====Neural Groove====

* forms in the midline of the neural plate (day 18-19) 
* either side of which are the neural folds which continues to deepen until about week 4 
* neural folds begins to fuse, beginning at 4th somite level 

====Neural Tube====
[[Image:Stage12 SEM3.jpg|300px|Stage 12 caudal neuropore]]
[[File:Neuraltube_001 icon.jpg|120px|link=Development Animation - Neural Tube]]
* the neural tube forms the brain and spinal cord 
* fusion of neural groove extends rostrally and caudally 
* begins at the level of 4th somite 
* closes neural groove "zips up" in some species.
** humans appear to close at multiple points along the tube.
* leaves 2 openings at either end - '''Neuropores''' 
** cranial neuropore closes before caudal

Failure for the neural tube to close correctly or completely results in a '''neural tube defect'''.

<gallery>
File:CNS primary vesicles.jpg|Neural - 3 primary vesicles
</gallery>
====Neural Crest====

* population of cells at the edge of the neural plate that lie dorsally when the neural tube fuses 
* dorsal to the neural tube, as a pair of streaks 
* pluripotential, forms many different types of cells 
* cells migrate throughout the embryo 

'''Neural Crest Derivatives''': dorsal root ganglia, autonomic ganglia, adrenal medulla, drg sheath cells, glia, pia-arachnoid sheath, skin melanocytes, connective tissue of cardiac outflow, thyroid parafollicular cells, craniofacial skeleton, teeth odontoblasts

===Head===
[[File:Stage14 sem2b-limb.jpg|thumb|Stage 14 pharyngeal arches]]

* branchial arch (Gk. ''branchia'' = gill) 
* arch consists of all 3 trilaminar embryo layers (ectoderm- outside, mesoderm - core of mesenchyme, endoderm - inside)

<gallery>
File:Pharyngeal arch structure cartoon.gif
File:Stage13 pharyngeal arch excerpts.gif
File:Head_arches_cartoon.jpg
</gallery>
* Humans have 5 arches - 1, 2, 3, 4, 6 (Arch 5 does not form or regresses  rapidly)
* from in rostro-caudal sequence, Arch 1 to 6  from week 4 onwards 
* arch 1 and 2 appear at time of closure of cranial neuropore 
* Face - mainly arch 1 and 2 
* Neck components - arch 3 and 4 (arch 4 and 6 fuse)
[[File:Face 001 icon.jpg|120px|link=Development_Animation_-_Face]]
====Sensory Placodes ====

* During week 4 a series of thickened surface ectodermal patches form in pairs rostro-caudally in the head region.
* These sensory placodes will later contribute key components of each of our special senses (vision, hearing and smell).
* Note that their initial postion on the developing head is significantly different to their final position in the future sensory system

* '''Otic placode''' - istage 13/14 embryo the otic placode sunk from the surface ectoderm to form a hollow epithelial ball, the '''otocyst''', which now lies beneath the surface surrounded by mesenchyme (mesoderm). The epithelia of this ball varies in thickness and has begun to distort, it will eventually form the inner ear membranous labyrinth.
* '''Lens placode''' - lies on the surface, adjacent to the outpocketing of the nervous system (which will for the retina) and will form the lens.
* '''Nasal placode''' - has 2 components (medial and lateral) and will form the nose olefactory epithelium.

===Upper and Lower Limb===
[[File:Stage14_somites_limbbuds.png|thumb]]
* Limb development occurs at different times for forelimbs and hindlimbs. 
*  mid-4th week human upper limb buds first
* lower limbs about 2 days later
* The limbs form at vertebra segmental levels C5-C8 (upper limbs) L3-L5 (lower limbs).
* Limbs are initially undifferentiated mesenchyme (mesoderm) with an epithelial (ectoderm) covering.
* Blood vessels then begin forming, the largest (marginal vein) is adjacent to tip of the bud.
* Myotome invade the bud.

===Gastrointestinal Tract===

* Begins at buccopharyngeal membrane
* Ends at cloacal membrane
* 3 distinct portions (fore-, mid- and hind-gut)
* liver earliest forming organ

'''Germ layer contributions'''
* '''Endoderm''' - epithelium and associated glands
* '''Mesoderm''' (splanchnic) - mesentry, connective tissues, smooth muscle, blood vessels
* '''Ectoderm''' (neural crest) - enteric nervous system

Both endoderm and mesoderm will contribute to associated organs.

<gallery>
File:Gray0982a.jpg|Gastrointestinal Tract
</gallery>

==Week 5==
<gallery>
File:Stage14_sem1c.jpg|Stage 14
File:Stage15_bf1c.jpg|Stage 15
File:Stage14_respiratory_tract.jpg|Respiratory Tract
File:CNS secondary vesicles.jpg|Neural - 5 secondary vesicles
</gallery>
* Heart - septation starts, atrial and ventricular 
* Vascular - 3 vascular systems (systemic, placental, vitelline) extensively remodelled
* Respiratory -  left and right lung buds push into the pericardioperitoneal canals (primordia of pleural cavity)
* Sense - Hearing cochlear part of otic vesicle elongates (humans 2.5 turns)

==Week 6==
<gallery>
File:Stage16_bf1c.jpg|Stage 16
File:Stage17_bf1c.jpg|Stage 17
</gallery>
* Endocrine development 
** Pituitary - connecting stalk between pouch and oral cavity degenerates
** Parathyroid  - diverticulum elongate, hollow then solid, dorsal cell proliferation
** Thymus  - diverticulum elongate, hollow then solid, ventral cell proliferation
** Adrenal - fetal cortex forms from mesothelium adjacent to dorsal mesentery, medulla neural crest cells from adjacent sympathetic ganglia

==Week 7==
<gallery>
File:Stage18_bf1c.jpg|Stage 18
File:Stage19_bf1c.jpg|Stage 19
</gallery>
* Pancreas - Week 7 to 20 pancreatic hormones secretion increases, small amount maternal insulin
* Limb bones form by endochondrial ossification and throughout embryo replacement of cartilage with bone (week 5 onward).
<gallery>
File:Endochondral_bone.jpg|Endochondral ossification in limb
File:Endochondral_ossification.jpg|Endochondral ossification
File:Fetal_head_medial.jpg|Head Intramembranous ossification
File:Ossification_centre.jpg|Intramembranous ossification
</gallery>

==Week 8==
<gallery>
File:Stage22_HPA2L.jpg|Neural - early developing cortex
File:Gray0986.jpg|Gastrointestinal tract herniation
</gallery>

* Limb upper and lower limbs rotate  in different directions (upper limb dorsally, lower limb ventrally)

[[File:Stage20-23 limbs a.jpg]]



'''Links:''' [[Embryonic Development]] | [[Timeline_human_development|Timeline human development]] |  [http://embryology.med.unsw.edu.au/Medicine/BGDlecture6.htm Previous 2008 Lecture]

==Fetal==
[[File:Fetal_length_and_weight_change.jpg|thumb|Fetal length and weight changes]]

* First Trimester (1 - 12 weeks) - embryonic and early fetal
* Second Trimester (13 - 24 weeks) - organ development and function, growth (length)
* Third Trimester (25 - 40 weeks) - organ function and rapid growth (weight)

===Fetal Neural===
[[File:Neural-development.jpg|thumb|Timeline of events in Human Neural Development]]

* During the fetal period there is ongoing growth in size, weight and surface area of the brain and spinal cord. Microscopically there is ongoing: cell migration, extension of processes, cell death and glial cell development.
* Brain - Insular cortex, Gyral and Sulcal development
* Neural development will continue after birth with substantial growth, death and reorganization occuring during the postnatal period

===Lung Stages===
* week 4 - 5 embryonic
* week 5 - 17 pseudoglandular
* week 16 - 25 canalicular
* week 24 - 40 terminal sac
* late fetal - 8 years alveolar

===Fetal Genital===
* ovary and testis development
* external genital development
* testis descent

===Fetal Endocrine===
* Pituitary Hormones - HPA axis established by week 20, pituitary functional throughout fetal development
* Thyroid Hormone - important for neural development, required for metabolic activity, also in the newborn

Remember that the Placenta also has important endocrine functions during development.

==Critical Periods==
{| class="prettytable"
| bgcolor= "LightCyan" colspan="2" | '''Conceptus'''
| bgcolor= "LightBlue" colspan="13" | '''Embryonic development''' (weeks)
| bgcolor= "LightSkyBlue" colspan="5" | '''Fetal period''' (weeks)

|-
| bgcolor= "LightCyan" width="50px" |<center>'''1'''</center>
| bgcolor= "LightCyan" width="50px" |<center>'''2'''</center>
| bgcolor= "LightBlue" width="50px" colspan="2" |<center>'''3'''</center>
| bgcolor= "LightBlue" width="50px" colspan="3" |<center>'''4'''</center>
| bgcolor= "LightBlue" width="50px" | <center>'''5'''</center>
| bgcolor= "LightBlue" width="50px" colspan="2" | <center>'''6'''</center>
| bgcolor= "LightBlue" width="50px" colspan="2" | <center>'''7'''</center>
| bgcolor= "LightBlue" width="50px" colspan="3" | <center>'''8'''</center>
| bgcolor= "LightSkyBlue" width="50px" colspan="2" | <center>'''9'''</center>
| bgcolor= "LightSkyBlue" width="50px" | <center>'''16'''</center>
| bgcolor= "LightSkyBlue" width="50px" | <center>'''20-36'''</center>
| bgcolor= "LightSkyBlue" width="50px" | <center>'''38'''</center>

|-
| bgcolor= "LightCyan" width="50px" | [[File:Early_zygote.jpg|50px]]
| bgcolor= "LightCyan" width="50px" | [[File:Week2_001 icon.jpg|50px]]
| bgcolor= "LightBlue" width="50px" colspan="2" |[[File:Stage9_sem4c.jpg|50px]]
| bgcolor= "LightBlue" width="50px" colspan="3" |[[File:Stage13_sem1c.jpg|50px]]
| bgcolor= "LightBlue" width="50px" |[[File:Stage15_bf1c.jpg|50px]]
| bgcolor= "LightBlue" width="50px" colspan="2" |[[File:Stage17_bf1c.jpg|50px]]
| bgcolor= "LightBlue" width="50px" colspan="2" |[[File:Stage19_bf1c.jpg|50px]]
| bgcolor= "LightBlue" width="50px" colspan="3" |[[File:Stage23_bf1c.jpg|50px]]
| bgcolor= "LightSkyBlue" width="50px" colspan="2" |
| bgcolor= "LightSkyBlue" width="50px" |
| bgcolor= "LightSkyBlue" width="50px" |
| bgcolor= "LightSkyBlue" width="50px" |

|-
| 
| 
| bgcolor= "Salmon" colspan="16" | '''Neural'''
| bgcolor= "LightSalmon" colspan="2" | 

|-
| [[File:Stage2.jpg|50px]]
| 
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| bgcolor= "Salmon" colspan="6" | '''Heart'''
| bgcolor= "LightSalmon" colspan="6" | 
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| bgcolor= "Salmon" colspan="6" | '''Upper limbs'''
| bgcolor= "LightSalmon" colspan="5" | 
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|-
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| bgcolor= "Salmon" colspan="6" | '''Lower limbs'''
| bgcolor= "LightSalmon" colspan="4" | 
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|-
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| bgcolor= "Salmon" colspan="9" | '''Ear'''
| bgcolor= "LightSalmon" colspan="4" | 
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|-
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| bgcolor= "Salmon" colspan="7" | '''Eye'''
| bgcolor= "LightSalmon" colspan="7" | 

|-
| [[File:CSt3.jpg|50px]]
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| bgcolor= "Salmon" colspan="7" | '''Palate'''
| bgcolor= "LightSalmon" |
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|-
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| bgcolor= "Salmon" colspan="6" | '''Teeth'''
| bgcolor= "LightSalmon" colspan="5" | 

|-
| [[File:Week2_001 icon.jpg|50px]]
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| bgcolor= "Salmon" colspan="5" | '''External genitalia'''
| bgcolor= "LightSalmon" colspan="4" | 

|-
| bgcolor= "LightCyan" colspan="2" | '''Loss'''
| bgcolor= "Salmon" colspan="10" | '''Major abnormalities'''
| bgcolor= "LightSalmon" colspan="8" | '''Functional and Minor abnormalities''' 

|}

[[File:Human-critical_periods.jpg|thumb]]

'''Links:''' [[Embryonic Development]] | [[Timeline_human_development|Timeline human development]] | [[Movie - Human Development annotated cartoon]] | [http://embryology.med.unsw.edu.au/Medicine/BGDlecture6.htm Previous 2008 Lecture]


{{Template:BGDFooter2010}}