Mouse Knockout - Revision history

Z8600021 at 02:08, 10 July 2015

2015-07-10T02:08:11Z

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	[[File:Mouse E0-E5.jpg\|thumb]]		[[File:Mouse E0-E5.jpg\|thumb]]
	The mouse (taxon-''mus'') has always been a good embryological model, generating easily (litters 8-20) and quickly (21d). Mouse embryology really expanded when molecular biologists used mice for gene knockouts. The term "knockout mouse" is a mouse where researchers have inactivated, or "knocked out," an existing gene by replacing it or disrupting it with an artificial piece of DNA. Currently there is a scientific drive to establish a knockout for all known mouse genes, and new technologies allow both "conditional knockouts" and "knockins".		The mouse (taxon-''mus'') has always been a good embryological model, generating easily (litters 8-20) and quickly (21d). Mouse embryology really expanded when molecular biologists used mice for gene knockouts. The term "knockout mouse" is a mouse where researchers have inactivated, or "knocked out," an existing gene by replacing it or disrupting it with an artificial piece of DNA. Currently there is a scientific drive to establish a knockout for all known mouse genes, and new technologies allow both "conditional knockouts" and "knockins".


			The new '''CRISPR''' (Clustered Regularly Interspaced Short Palindromic Repeats) technique will make significant differences in the ability to quickly and easily generate new knockout animal models.


	:''Now it is necessary to understand development, in order to understand the effect of knocking out these gene.''		:''Now it is necessary to understand development, in order to understand the effect of knocking out these gene.''
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	* [https://www.komp.org/geneinfo.php?geneid=87831 Zzef1] zinc finger, ZZ-type with EF hand domain 1		* [https://www.komp.org/geneinfo.php?geneid=87831 Zzef1] zinc finger, ZZ-type with EF hand domain 1

			==References==

			<references/>

	== External Links ==		== External Links ==

Z8600021 at 02:05, 10 July 2015

2015-07-10T02:05:12Z

← Older revision		Revision as of 13:05, 10 July 2015
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	{{Header}}		{{Header}}
	[[File:Mouse.jpg\|~~left~~]]		[[File:Mouse.jpg\|right]]
	== Introduction ==		== Introduction ==
	[[File:Mouse E0-E5.jpg\|thumb]]		[[File:Mouse E0-E5.jpg\|thumb]]

Z8600021 at 02:04, 10 July 2015

2015-07-10T02:04:34Z

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Z8600021 at 01:59, 10 July 2015

2015-07-10T01:59:53Z

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Z8600021 at 23:53, 25 March 2015

2015-03-25T23:53:24Z

← Older revision		Revision as of 10:53, 26 March 2015
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			* '''Restoration of Spermatogenesis and Male Fertility Using an Androgen Receptor Transgene'''<ref name="PMID25803277"><pubmed>25803277</pubmed></ref> "Androgens signal through the androgen receptor (AR) to regulate male secondary sexual characteristics, reproductive tract development, prostate function, sperm production, bone and muscle mass as well as body hair growth among other functions. We developed a transgenic mouse model in which endogenous AR expression was replaced by a functionally modified AR transgene. A bacterial artificial chromosome (BAC) was constructed containing all AR exons and introns plus 40 kb each of 5' and 3' regulatory sequence. Insertion of an internal ribosome entry site and the EGFP gene 3' to AR allowed co-expression of AR and EGFP. Pronuclear injection of the BAC resulted in six founder mice that displayed EGFP production in appropriate AR expressing tissues. The six founder mice were mated into a Sertoli cell specific AR knockout (SCARKO) background in which spermatogenesis is blocked at the meiosis stage of germ cell development. The AR-EGFP transgene was expressed in a cyclical manner similar to that of endogenous AR in Sertoli cells and fertility was restored as offspring were produced in the absence of Sertoli cell AR. Thus, the AR-EGFP transgene under the control of AR regulatory elements is capable of rescuing AR function in a cell selective, AR-null background. These initial studies provide proof of principle that a strategy employing the AR-EGFP transgene can be used to understand AR functions."
	* '''Mouse library set to be knockout'''<ref><pubmed>21677718</pubmed>\| [http://www.nature.com/news/2011/110615/full/474262a.html Nature News]</ref> "Launched in 2006 in North America and Europe, the effort aims to disable each of the 20,000-odd genes in the mouse genome and make the resulting cell lines available to the scientific community."		* '''Mouse library set to be knockout'''<ref><pubmed>21677718</pubmed>\| [http://www.nature.com/news/2011/110615/full/474262a.html Nature News]</ref> "Launched in 2006 in North America and Europe, the effort aims to disable each of the 20,000-odd genes in the mouse genome and make the resulting cell lines available to the scientific community."
	* '''A conditional knockout resource for the genome-wide study of mouse gene function'''<ref name="PMID21677750"><pubmed>21677750</pubmed></ref> "Gene targeting in embryonic stem cells has become the principal technology for manipulation of the mouse genome, offering unrivalled accuracy in allele design and access to conditional mutagenesis. To bring these advantages to the wider research community, large-scale mouse knockout programmes are producing a permanent resource of targeted mutations in all protein-coding genes. Here we report the establishment of a high-throughput gene-targeting pipeline for the generation of reporter-tagged, conditional alleles. Computational allele design, 96-well modular vector construction and high-efficiency gene-targeting strategies have been combined to mutate genes on an unprecedented scale. So far, more than 12,000 vectors and 9,000 conditional targeted alleles have been produced in highly germline-competent C57BL/6N embryonic stem cells. High-throughput genome engineering highlighted by this study is broadly applicable to rat and human stem cells and provides a foundation for future genome-wide efforts aimed at deciphering the function of all genes encoded by the mammalian genome."		* '''A conditional knockout resource for the genome-wide study of mouse gene function'''<ref name="PMID21677750"><pubmed>21677750</pubmed></ref> "Gene targeting in embryonic stem cells has become the principal technology for manipulation of the mouse genome, offering unrivalled accuracy in allele design and access to conditional mutagenesis. To bring these advantages to the wider research community, large-scale mouse knockout programmes are producing a permanent resource of targeted mutations in all protein-coding genes. Here we report the establishment of a high-throughput gene-targeting pipeline for the generation of reporter-tagged, conditional alleles. Computational allele design, 96-well modular vector construction and high-efficiency gene-targeting strategies have been combined to mutate genes on an unprecedented scale. So far, more than 12,000 vectors and 9,000 conditional targeted alleles have been produced in highly germline-competent C57BL/6N embryonic stem cells. High-throughput genome engineering highlighted by this study is broadly applicable to rat and human stem cells and provides a foundation for future genome-wide efforts aimed at deciphering the function of all genes encoded by the mammalian genome."

Z8600021 at 23:49, 25 March 2015

2015-03-25T23:49:54Z

← Older revision		Revision as of 10:49, 26 March 2015
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			{{Header}}
	[[File:Mouse.jpg\|left]]		[[File:Mouse.jpg\|left]]
	== Introduction ==		== Introduction ==
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	:''Now it is necessary to understand development, in order to understand the effect of knocking out these gene.''		:''Now it is necessary to understand development, in order to understand the effect of knocking out these gene.''



	--[[User:Z8600021\|Mark Hill]] ([[User talk:Z8600021\|talk]]) 08:35, 24 June 2014 (EST) Note the external links below no longer function. The database has been relocated. I will remove this notice when links are repaired.		--[[User:Z8600021\|Mark Hill]] ([[User talk:Z8600021\|talk]]) 08:35, 24 June 2014 (EST) Note the external links below no longer function. The database has been relocated. I will remove this notice when links are repaired.

			{{External Links}}


	{{~~Template:~~Mouse}} \| [[Molecular Development - Genetics]]		{{Mouse}} \| [[Molecular Development - Genetics]]

	{{~~Template:~~Mouse E days}}		{{Mouse E days}}

	== Some Recent Findings ==		== Some Recent Findings ==
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	* [http://www.komp.org KOMP] The University of California, Davis (UC Davis) and Children's Hospital Oakland Research Institute (CHORI) will collaborate to preserve, protect and make available knockout mice and related products available to the research community. The repository will archive, maintain and distribute up to 8,500 strains of embryonic stem cell clones, live mouse lines, frozen embryos and sperm and vectors.		* [http://www.komp.org KOMP] The University of California, Davis (UC Davis) and Children's Hospital Oakland Research Institute (CHORI) will collaborate to preserve, protect and make available knockout mice and related products available to the research community. The repository will archive, maintain and distribute up to 8,500 strains of embryonic stem cell clones, live mouse lines, frozen embryos and sperm and vectors.

	{{~~Template:~~Animals}}		{{Animals}}




	{{~~Template:~~Glossary}}		{{Glossary}}

	{{~~Template:~~Footer}}		{{Footer}}

	[[Category:Mouse]] [[Category:Genetics]] [[Category:Molecular]]		[[Category:Mouse]] [[Category:Genetics]] [[Category:Molecular]]

Z8600021 at 22:35, 23 Haziran 2014

2014-06-23T22:35:25Z

← Older revision		Revision as of 09:35, 24 Haziran 2014
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	:''Now it is necessary to understand development, in order to understand the effect of knocking out these gene.''		:''Now it is necessary to understand development, in order to understand the effect of knocking out these gene.''


			--[[User:Z8600021\|Mark Hill]] ([[User talk:Z8600021\|talk]]) 08:35, 24 June 2014 (EST) Note the external links below no longer function. The database has been relocated. I will remove this notice when links are repaired.

Z8600021 at 22:29, 23 Haziran 2014

2014-06-23T22:29:51Z

← Older revision		Revision as of 09:29, 24 Haziran 2014
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	* '''A conditional knockout resource for the genome-wide study of mouse gene function'''<ref name="PMID21677750"><pubmed>21677750</pubmed></ref> "Gene targeting in embryonic stem cells has become the principal technology for manipulation of the mouse genome, offering unrivalled accuracy in allele design and access to conditional mutagenesis. To bring these advantages to the wider research community, large-scale mouse knockout programmes are producing a permanent resource of targeted mutations in all protein-coding genes. Here we report the establishment of a high-throughput gene-targeting pipeline for the generation of reporter-tagged, conditional alleles. Computational allele design, 96-well modular vector construction and high-efficiency gene-targeting strategies have been combined to mutate genes on an unprecedented scale. So far, more than 12,000 vectors and 9,000 conditional targeted alleles have been produced in highly germline-competent C57BL/6N embryonic stem cells. High-throughput genome engineering highlighted by this study is broadly applicable to rat and human stem cells and provides a foundation for future genome-wide efforts aimed at deciphering the function of all genes encoded by the mammalian genome."		* '''A conditional knockout resource for the genome-wide study of mouse gene function'''<ref name="PMID21677750"><pubmed>21677750</pubmed></ref> "Gene targeting in embryonic stem cells has become the principal technology for manipulation of the mouse genome, offering unrivalled accuracy in allele design and access to conditional mutagenesis. To bring these advantages to the wider research community, large-scale mouse knockout programmes are producing a permanent resource of targeted mutations in all protein-coding genes. Here we report the establishment of a high-throughput gene-targeting pipeline for the generation of reporter-tagged, conditional alleles. Computational allele design, 96-well modular vector construction and high-efficiency gene-targeting strategies have been combined to mutate genes on an unprecedented scale. So far, more than 12,000 vectors and 9,000 conditional targeted alleles have been produced in highly germline-competent C57BL/6N embryonic stem cells. High-throughput genome engineering highlighted by this study is broadly applicable to rat and human stem cells and provides a foundation for future genome-wide efforts aimed at deciphering the function of all genes encoded by the mammalian genome."
	\|}		\|}
			{\| class="wikitable mw-collapsible mw-collapsed"
			! More recent papers
			\|-
			\| [[File:Mark_Hill.jpg\|90px\|left]] {{Most_Recent_Refs}}

			Search term: ''Knockout Mouse''

			<pubmed limit=5>Knockout Mouse</pubmed>
			\|}
	==Mouse Gene Knockouts Listed according to the Name of the Gene==		==Mouse Gene Knockouts Listed according to the Name of the Gene==

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	* [http://www.bioscience.org/knockout/5-lipoxy.htm 5-Lipoxygenase-activating protein (FLAP)]		* [http://www.bioscience.org/knockout/5-lipoxy.htm 5-Lipoxygenase-activating protein (FLAP)]

			==A==
	* [http://www.bioscience.org/knockout/gaa.htm Acid Alpha-glucosidase gene (Gaa)]		* [http://www.bioscience.org/knockout/gaa.htm Acid Alpha-glucosidase gene (Gaa)]
	* [http://www.bioscience.org/knockout/acrosin.htm Acrosin]		* [http://www.bioscience.org/knockout/acrosin.htm Acrosin]
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	* [http://www.bioscience.org/knockout/atm.htm Atm]		* [http://www.bioscience.org/knockout/atm.htm Atm]

			==B==
	* [http://www.bioscience.org/knockout/b7.htm B7]		* [http://www.bioscience.org/knockout/b7.htm B7]
	* [http://www.bioscience.org/knockout/bax.htm Bax]		* [http://www.bioscience.org/knockout/bax.htm Bax]
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	* [http://www.bioscience.org/knockout/bdnf.htm Brain-derived neurotrophic factor (BDNF)]		* [http://www.bioscience.org/knockout/bdnf.htm Brain-derived neurotrophic factor (BDNF)]

			==C==
	* [http://www.bioscience.org/knockout/c5arecep.htm C5a receptor]		* [http://www.bioscience.org/knockout/c5arecep.htm C5a receptor]
	* [http://www.bioscience.org/knockout/c-abl.htm c-abl]		* [http://www.bioscience.org/knockout/c-abl.htm c-abl]
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	* [http://www.bioscience.org/knockout/cftr.htm Cystic fibrosis transmembrane conductance regulator (CFTR)]		* [http://www.bioscience.org/knockout/cftr.htm Cystic fibrosis transmembrane conductance regulator (CFTR)]

			==D==
	* [http://www.bioscience.org/knockout/doparec.htm D1A dopamine receptors]		* [http://www.bioscience.org/knockout/doparec.htm D1A dopamine receptors]
	* [http://www.bioscience.org/knockout/dazla.htm Dazla]		* [http://www.bioscience.org/knockout/dazla.htm Dazla]
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	* [http://www.bioscience.org/knockout/dv11.htm Dv11]		* [http://www.bioscience.org/knockout/dv11.htm Dv11]

			==E==
	* [http://www.bioscience.org/knockout/e2a.htm E2a]		* [http://www.bioscience.org/knockout/e2a.htm E2a]
	* [http://www.bioscience.org/knockout/e2f-1.htm E2f-1]		* [http://www.bioscience.org/knockout/e2f-1.htm E2f-1]

S8600021: /* Introduction */

2011-06-23T00:24:16Z

Introduction

← Older revision		Revision as of 11:24, 23 Haziran 2011
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	{{Template:Mouse}} \| [~~http://embryology.med.unsw.edu.au/OtherEmb/Mouse.htm original page~~]		{{Template:Mouse}} \| [[Molecular Development - Genetics]]

	{{Template:Mouse E days}}		{{Template:Mouse E days}}

S8600021: /* External Links */

2011-06-23T00:23:28Z

External Links

← Older revision		Revision as of 11:23, 23 Haziran 2011
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	{{Template:Footer}}		{{Template:Footer}}

	[[Category:Mouse]] [[Category:~~Genetic~~]] [[Category:Molecular]]		[[Category:Mouse]] [[Category:Genetics]] [[Category:Molecular]]