Histology Fixatives - Revision history

Z8600021 at 09:21, 5 October 2020

2020-10-05T09:21:49Z

← Older revision		Revision as of 19:21, 5 October 2020
Line 172:		Line 172:

	==Bouin==		==Bouin==
			{{Bouin}}

	Bouin’s Fixative (Bouin’s Fluid, Bouin solution)		Bouin’s Fixative (Bouin’s Fluid, Bouin solution)

Z8600021 at 07:51, 5 October 2020

2020-10-05T07:51:17Z

← Older revision		Revision as of 17:51, 5 October 2020
Line 126:		Line 126:

	=== Formalin ===		=== Formalin ===
			{{Formalin}}

	* Aldehyde Cross-Link fixation		* Aldehyde Cross-Link fixation
	* Formalin is a 37% aqueous solution of formaldehyde, which fixes by cross-linking like other aldehyde fixatives and is suitable for most histological purposes		* Formalin is a 37% aqueous solution of formaldehyde, which fixes by cross-linking like other aldehyde fixatives and is suitable for most histological purposes
Line 185:		Line 187:
	Do not use Bouin’s to fix before in situ hybridisation, as then unable to detect RNA.		Do not use Bouin’s to fix before in situ hybridisation, as then unable to detect RNA.

	~~==Formalin==~~

	==Kaiserling==		==Kaiserling==

Z8600021 at 07:21, 25 February 2017

2017-02-25T07:21:49Z

← Older revision		Revision as of 17:21, 25 February 2017
Line 17:		Line 17:

	{{Histology Links}}		{{Histology Links}}


	==Health and Safety (HS) ==		==Health and Safety (HS) ==

Z8600021 at 11:07, 9 November 2015

2015-11-09T11:07:54Z

← Older revision		Revision as of 21:07, 9 November 2015
Line 1:		Line 1:
			{{Header}}
	== Introduction==		== Introduction==

Z8600021 at 00:49, 22 July 2015

2015-07-22T00:49:38Z

← Older revision		Revision as of 10:49, 22 July 2015
Line 165:		Line 165:
	** hypertonic solutions may cause cells to shrink.		** hypertonic solutions may cause cells to shrink.
	** hypotonic solutions may cause the cells may swell and burst.		** hypotonic solutions may cause the cells may swell and burst.


			==Bouin==
			Bouin’s Fixative (Bouin’s Fluid, Bouin solution)

			Bouin’s fixative is the primary mordant solution, substance used to set a dye, in the trichrome stains. It is a compound fixative composed of picric acid, acetic acid and formaldehyde in an aqueous solution. Picric acid acts to balance the fixation effects of the other 2 agents.
			* Acetic acid tissue swelling effect is balanced by the tissue shrinking effect of picric acid.
			* Formalin causes cytoplasm to become basophilic effect is balanced by the picric acid. Good for nuclear and cytoplasmic {{HE}} staining.
			* Formalin causes tissue hardening effect is balanced by the soft tissue fixation of picric acid.
			* Picric acid when retained interferes with some stains, including those used for blood cells.

			Whole specimen fixation
			* small specimens for at least 6 hours.
			* other specimens can be fixed up to two days.
			* large specimens can be fixed for up to three days.

			Do not use Bouin’s to fix before in situ hybridisation, as then unable to detect RNA.

			==Formalin==

			==Kaiserling==
			(Kaiserling’s preservative, Kaiserling’s fixative) An aqueous solution of potassium nitrate, potassium acetate, and formalin. There are four preservative variants (Kaiserling 1896, 1899, Kaiserling I and II), two are used in anatomy museums (Kaiserling I and II). Johann Carl Kaiserling (1869 - 1942) was a German pathologist.

Z8600021: /* Occupational Health and Safety (OHS) */

2012-02-28T13:30:18Z

Occupational Health and Safety (OHS)

← Older revision		Revision as of 23:30, 28 February 2012
Line 17:		Line 17:
	{{Histology Links}}		{{Histology Links}}

	== ~~Occupational~~ Health and Safety (~~OHS~~) ==		==Health and Safety (HS) ==

	* [http://medicalsciences.med.unsw.edu.au/SOMSWeb.nsf/page/~~OHS~~ School of Medical Sciences, ~~Occupational~~ Health and Safety (~~OHS~~) Committee]		* [http://medicalsciences.med.unsw.edu.au/SOMSWeb.nsf/page/Health+Safety School of Medical Sciences, Health and Safety (H&S) Committee]
	** "To facilitate a safe work environment by developing and documenting ~~OHS~~ programs to coordinate training of staff and students and by overseeing the implementation of ~~OHS~~ procedures and policies in the School of Medical Sciences."		** "To facilitate a safe work environment by developing and documenting H&S programs to coordinate training of staff and students and by overseeing the implementation of H&S procedures and policies in the School of Medical Sciences."
	* [http://medicalsciences.med.unsw.edu.au/SOMSWeb.nsf/page/Acts%20and%20Standards Australian Acts and Standards]		* [http://medicalsciences.med.unsw.edu.au/SOMSWeb.nsf/page/Acts%20and%20Standards Australian Acts and Standards]
	* An ~~OHS~~ Management System (OHSMS) is a set of plans, actions and procedures to systematically manage health and safety in the workplace that is actively endorsed by a committed employer to achieve:		* An H&S Management System (OHSMS) is a set of plans, actions and procedures to systematically manage health and safety in the workplace that is actively endorsed by a committed employer to achieve:
	** Provision of a safe and health workplace and the prevention/reduction of illness and injury equally for employees and contractors.		** Provision of a safe and health workplace and the prevention/reduction of illness and injury equally for employees and contractors.
	** Identification of workplace hazards, assessment and control of all risks.		** Identification of workplace hazards, assessment and control of all risks.

S8600021: /* Introduction */

2011-08-29T22:12:34Z

Introduction

← Older revision		Revision as of 08:12, 30 August 2011
Line 15:		Line 15:


	~~:'''~~Links~~:''' [[Histology Stains]] \| [[Histology Fixatives]] \| [[:Category:Histology\|Category:Histology]]~~		{{Histology Links}}

	== Occupational Health and Safety (OHS) ==		== Occupational Health and Safety (OHS) ==

S8600021 at 21:49, 29 August 2011

2011-08-29T21:49:08Z

← Older revision		Revision as of 07:49, 30 August 2011
Line 15:		Line 15:


	:'''Links:''' [[Histology Stains]] \| [[:Category:Histology\|Category:Histology]]		:'''Links:''' [[Histology Stains]] \| [[Histology Fixatives]] \| [[:Category:Histology\|Category:Histology]]

	== Occupational Health and Safety (OHS) ==		== Occupational Health and Safety (OHS) ==

S8600021: /* Introduction */

2011-08-29T21:48:09Z

Introduction

← Older revision		Revision as of 07:48, 30 August 2011
Line 1:		Line 1:
	== Introduction==		== Introduction==

	It is critical to match the method of fixation with the intended analytical technique. Some types of analysis are totally incompatible with certain fixation techniques and always consider that "artefacts" can be introduced by the fixation process. Fixation methods discussed on this page include: fresh frozen, precipitation and aldehyde cross-linking. ~~This content is covered~~ in ~~a [http://php~~.~~med.unsw.edu.au/cellbiology/index.php?title=2011_Lab_3 practical lab] as part~~ of ~~an undergraduate science cell biology course~~.		It is critical to match the method of fixation with the intended analytical technique. Some types of analysis are totally incompatible with certain fixation techniques and always consider that "artefacts" can be introduced by the fixation process. Fixation methods discussed on this page include: fresh frozen, precipitation and aldehyde cross-linking. Always consider the Occupational Health and Safety (OHS) issues in relation to chemicals used in this process. More information is available from the School of Medical Sciences OHS webpage.

	~~Always consider the Occupational Health and Safety (OHS) issues~~ in ~~relation to chemicals used in this process~~. ~~More information is available from the School~~ of ~~Medical Sciences OHS webpage~~.

			This content is covered in a [http://php.med.unsw.edu.au/cellbiology/index.php?title=2011_Lab_3 practical lab] as part of an undergraduate science cell biology course.

	In general the Fixation process should:		In general the Fixation process should:

S8600021: /* Introduction */

2011-08-29T21:46:03Z

Introduction

← Older revision		Revision as of 07:46, 30 August 2011
Line 11:		Line 11:
	# Make the tissue resistant to damage during subsequent processing (hardy)		# Make the tissue resistant to damage during subsequent processing (hardy)

	:Links: [[Histology Stains]] \| [[:Category:Histology\|Category:Histology]]

			:'''Links:''' [[Histology Stains]] \| [[:Category:Histology\|Category:Histology]]

	== Occupational Health and Safety (OHS) ==		== Occupational Health and Safety (OHS) ==