File:Zebrafish Wdr18 expression 01.jpg - Revision history

Z8600021 at 23:15, 3 December 2014

2014-12-03T23:15:44Z

← Older revision		Revision as of 10:15, 4 December 2014
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	* '''A''' - wdr18 is maternally expressed.		* '''A''' - wdr18 is maternally expressed.
	* '''B''' - Expression pattern of wdr18 at the 75%-epiboly stage. Black arrow indicates the staining of dorsal forerunner cells (DFCs).		* '''B''' - Expression pattern of wdr18 at the 75%-epiboly stage. Black arrow indicates the staining of dorsal forerunner cells (DFCs).
	* '''C''' - wdr18 expression is detected around the Kupffer's vesicle (white arrowhead) at the 6-somite stage. Inset is an overexposed magnified image showing elevated wdr18 expression around the KV. (See also [[:File:Zebrafish Wdr18 expression 01.jpg\|detailed image]])		* '''C''' - wdr18 expression is detected around the Kupffer's vesicle (white arrowhead) at the 6-somite stage. Inset is an overexposed magnified image showing elevated wdr18 expression around the KV. (See also [[:File:Zebrafish Wdr18 expression 02.jpg\|detailed image]])
	* '''D''' - wdr18 is detected ubiquitously throughout the whole embryo at 18-somite stage.		* '''D''' - wdr18 is detected ubiquitously throughout the whole embryo at 18-somite stage.
	* '''E''' - At 3 dpf, wdr18 is expressed in endodermal organs, including the exocrine pancreas (black arrow), liver (white arrow), and intestine.		* '''E''' - At 3 dpf, wdr18 is expressed in endodermal organs, including the exocrine pancreas (black arrow), liver (white arrow), and intestine.
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			:'''Links:''' [[:File:Zebrafish Wdr18 expression 01.jpg\|Image - full figure]] \| [[:File:Zebrafish Wdr18 expression 02.jpg\|Image - Panel C]] \| [[Zebrafish Development]]
	===Reference===		===Reference===
	<pubmed>21876750</pubmed>\| [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023386 PLoS One.]		<pubmed>21876750</pubmed>\| [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023386 PLoS One.]

Z8600021 at 23:13, 3 December 2014

2014-12-03T23:13:30Z

← Older revision		Revision as of 10:13, 4 December 2014
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	* '''A''' - wdr18 is maternally expressed.		* '''A''' - wdr18 is maternally expressed.
	* '''B''' - Expression pattern of wdr18 at the 75%-epiboly stage. Black arrow indicates the staining of dorsal forerunner cells (DFCs).		* '''B''' - Expression pattern of wdr18 at the 75%-epiboly stage. Black arrow indicates the staining of dorsal forerunner cells (DFCs).
	* '''C''' - wdr18 expression is detected around the Kupffer's vesicle (white arrowhead) at the 6-somite stage. Inset is an overexposed magnified image showing elevated wdr18 expression around the KV.		* '''C''' - wdr18 expression is detected around the Kupffer's vesicle (white arrowhead) at the 6-somite stage. Inset is an overexposed magnified image showing elevated wdr18 expression around the KV. (See also [[:File:Zebrafish Wdr18 expression 01.jpg\|detailed image]])
	* '''D''' - wdr18 is detected ubiquitously throughout the whole embryo at 18-somite stage.		* '''D''' - wdr18 is detected ubiquitously throughout the whole embryo at 18-somite stage.
	* '''E''' - At 3 dpf, wdr18 is expressed in endodermal organs, including the exocrine pancreas (black arrow), liver (white arrow), and intestine.		* '''E''' - At 3 dpf, wdr18 is expressed in endodermal organs, including the exocrine pancreas (black arrow), liver (white arrow), and intestine.

Z8600021 at 23:12, 3 December 2014

2014-12-03T23:12:09Z

← Older revision		Revision as of 10:12, 4 December 2014
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	==Zebrafish Wdr18 ~~expression~~==		==Zebrafish Wdr18 Expression==


	(A) wdr18 is maternally expressed. (B) Expression pattern of wdr18 at the 75%-epiboly stage. Black arrow indicates the staining of dorsal forerunner cells (DFCs). (C) wdr18 expression is detected around the Kupffer's vesicle (white arrowhead) at the 6-somite stage. Inset is an overexposed magnified image showing elevated wdr18 expression around the KV. (D) wdr18 is detected ubiquitously throughout the whole embryo at 18-somite stage. (E) At 3 dpf, wdr18 is expressed in endodermal organs, including the exocrine pancreas (black arrow), liver (white arrow), and intestine.		* '''A''' - wdr18 is maternally expressed.
			* '''B''' - Expression pattern of wdr18 at the 75%-epiboly stage. Black arrow indicates the staining of dorsal forerunner cells (DFCs).
			* '''C''' - wdr18 expression is detected around the Kupffer's vesicle (white arrowhead) at the 6-somite stage. Inset is an overexposed magnified image showing elevated wdr18 expression around the KV.
			* '''D''' - wdr18 is detected ubiquitously throughout the whole embryo at 18-somite stage.
			* '''E''' - At 3 dpf, wdr18 is expressed in endodermal organs, including the exocrine pancreas (black arrow), liver (white arrow), and intestine.


	Wdr18 is a member of the WD-repeat protein family found in all eukaryotes. The WD repeat consists of a 44 to 60 amino acid residue sequence that typically contains a GH dipeptide located 11 to 24 residues from its N-terminus and ends with a WD dipeptide at its C-terminus. Wdr18 is maternally expressed from the 1–4 cell stage.		'''Wdr18''' - is a member of the WD-repeat protein family found in all eukaryotes. The WD repeat consists of a 44 to 60 amino acid residue sequence that typically contains a GH dipeptide located 11 to 24 residues from its N-terminus and ends with a WD dipeptide at its C-terminus. Wdr18 is maternally expressed from the 1–4 cell stage.


			===Reference===
			<pubmed>21876750</pubmed>\| [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023386 PLoS One.]
	~~PLoS One. 2011;6(8):e23386. doi: 10.1371~~/~~journal.pone.0023386. Epub 2011 Aug 18.~~
	~~Wdr18 is required for Kupffer's vesicle formation and regulation of body asymmetry in zebrafish.~~
	~~Gao W1, Xu L, Guan R, Liu X, Han Y, Wu Q, Xiao Y, Qi F, Zhu Z, Lin S, Zhang B.~~
	~~Author information~~
	~~Abstract~~
	Correct specification of the left-right (L-R) axis is important for organ morphogenesis. Conserved mechanisms involving cilia rotation inside node-like structures and asymmetric Nodal signaling in the lateral plate mesoderm (LPM), which are important symmetry-breaking events, have been intensively studied. In zebrafish, the clustering and migration of dorsal forerunner cells (DFCs) is critical for the formation of the Kuppfer's vesicle (KV). However, molecular events underlying DFC clustering and migration are less understood. The WD-repeat proteins function in a variety of biological processes, including cytoskeleton assembly, intracellular trafficking, mRNA splicing, transcriptional regulation and cell migration. However, little is known about the function of WD-repeat proteins in L-R asymmetry determination. Here, we report the identification and functional analyses of zebrafish wdr18, a novel gene that encodes a WD-repeat protein that is highly conserved among vertebrate species. wdr18 was identified from a Tol2 transposon-mediated enhancer trap screen. Follow-up analysis of wdr18 mRNA expression showed that it was detected in DFCs or the KV progenitor cells and later in the KV at early somitogenesis stages. Morpholino knockdown of wdr18 resulted in laterality defects in the visceral organs, which were preceded by the mis-expression of Nodal-related genes, including spaw and pitx2. Examination of morphants at earlier stages revealed that the KV had fewer and shorter cilia which are immotile and a smaller cavity. We further investigated the organization of DFCs in wdr18 morphant embryos using ntl and sox17 as specific markers and found that the clustering and migration of DFC was altered, leading to a disorganized KV. Finally, through a combination of wdr18 and itgb1b morpholino injections, we provided evidence that wdr18 and itgb1b genetically interact in the laterality determination process. Thus, we reveal a new and essential role for WD-repeat proteins in the determination and regulation of L-R asymmetry and propose a potential mechanism for wdr18 in the regulation of DFC clustering and migration and KV formation.
	~~PMID 21876750~~

	http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023386

	====Copyright====		====Copyright====

Z8600021: ==Zebrafish Wdr18 expression== (A) wdr18 is maternally expressed. (B) Expression pattern of wdr18 at the 75%-epiboly stage. Black arrow indicates the staining of dorsal forerunner cells (DFCs). (C) wdr18 expression is detected around the Kupffer's ve...

2014-12-03T23:04:27Z

New page

==Zebrafish Wdr18 expression==

(A) wdr18 is maternally expressed. (B) Expression pattern of wdr18 at the 75%-epiboly stage. Black arrow indicates the staining of dorsal forerunner cells (DFCs). (C) wdr18 expression is detected around the Kupffer's vesicle (white arrowhead) at the 6-somite stage. Inset is an overexposed magnified image showing elevated wdr18 expression around the KV. (D) wdr18 is detected ubiquitously throughout the whole embryo at 18-somite stage. (E) At 3 dpf, wdr18 is expressed in endodermal organs, including the exocrine pancreas (black arrow), liver (white arrow), and intestine.

Wdr18 is a member of the WD-repeat protein family found in all eukaryotes. The WD repeat consists of a 44 to 60 amino acid residue sequence that typically contains a GH dipeptide located 11 to 24 residues from its N-terminus and ends with a WD dipeptide at its C-terminus. Wdr18 is maternally expressed from the 1–4 cell stage.

PLoS One. 2011;6(8):e23386. doi: 10.1371/journal.pone.0023386. Epub 2011 Aug 18.
Wdr18 is required for Kupffer's vesicle formation and regulation of body asymmetry in zebrafish.
Gao W1, Xu L, Guan R, Liu X, Han Y, Wu Q, Xiao Y, Qi F, Zhu Z, Lin S, Zhang B.
Author information
Abstract
Correct specification of the left-right (L-R) axis is important for organ morphogenesis. Conserved mechanisms involving cilia rotation inside node-like structures and asymmetric Nodal signaling in the lateral plate mesoderm (LPM), which are important symmetry-breaking events, have been intensively studied. In zebrafish, the clustering and migration of dorsal forerunner cells (DFCs) is critical for the formation of the Kuppfer's vesicle (KV). However, molecular events underlying DFC clustering and migration are less understood. The WD-repeat proteins function in a variety of biological processes, including cytoskeleton assembly, intracellular trafficking, mRNA splicing, transcriptional regulation and cell migration. However, little is known about the function of WD-repeat proteins in L-R asymmetry determination. Here, we report the identification and functional analyses of zebrafish wdr18, a novel gene that encodes a WD-repeat protein that is highly conserved among vertebrate species. wdr18 was identified from a Tol2 transposon-mediated enhancer trap screen. Follow-up analysis of wdr18 mRNA expression showed that it was detected in DFCs or the KV progenitor cells and later in the KV at early somitogenesis stages. Morpholino knockdown of wdr18 resulted in laterality defects in the visceral organs, which were preceded by the mis-expression of Nodal-related genes, including spaw and pitx2. Examination of morphants at earlier stages revealed that the KV had fewer and shorter cilia which are immotile and a smaller cavity. We further investigated the organization of DFCs in wdr18 morphant embryos using ntl and sox17 as specific markers and found that the clustering and migration of DFC was altered, leading to a disorganized KV. Finally, through a combination of wdr18 and itgb1b morpholino injections, we provided evidence that wdr18 and itgb1b genetically interact in the laterality determination process. Thus, we reveal a new and essential role for WD-repeat proteins in the determination and regulation of L-R asymmetry and propose a potential mechanism for wdr18 in the regulation of DFC clustering and migration and KV formation.
PMID 21876750

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023386

====Copyright====
© 2011 Gao et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Figure 1. journal.pone.0023386.g001.jpg doi:10.1371/journal.pone.0023386.g001 original figure altered in size and labeling.

[[Category:Zebrafish]]