File:Thalidomide - CPS49 vascular effect.jpg - Revision history

Z8600021 at 03:24, 11 April 2019

2019-04-11T03:24:13Z

← Older revision		Revision as of 14:24, 11 April 2019
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	CPS49 - a tetrafluorinated analogue of thalidomide that is chemically and structurally related to thalidomide breakdown products.		CPS49 - a tetrafluorinated analogue of thalidomide that is chemically and structurally related to thalidomide breakdown products.

	(A–F) Confocal images of zebrafish intersomitic vessel (ISV) formation 3 h (A–C) or 6 h (D–F) after DMSO (A and D) or CPS49 (B, C, E, and F) treatment at 24 h postfertilization.		('''A–F''') Confocal images of {{zebrafish}} intersomitic vessel (ISV) formation 3 h (A–C) or 6 h (D–F) after DMSO (A and D) or CPS49 (B, C, E, and F) treatment at 24 h postfertilization.

	Black arrowheads in B and E are shown at higher power in C and F. CPS49 reduced the number of filopodia and filopodial extensions (arrowheads), stunted forming ISVs (black asterisk), and prevented formation of a patent, functional vascular network (black arrows; compare A, B, D, and E).		Black arrowheads in B and E are shown at higher power in C and F. CPS49 reduced the number of filopodia and filopodial extensions (arrowheads), stunted forming ISVs (black asterisk), and prevented formation of a patent, functional vascular network (black arrows; compare A, B, D, and E).

	(G and H) Number of tip cell filopodia (G) and accumulative change in ISV length (H) in control (DMSO; open circles) and CPS49 (filled circles) treated zebrafish embryos imaged by using time-lapse photography.

	(~~I–L~~) ~~Confocal images~~ of ~~HUVEC cultured cells after 1 h of treatment with DMSO~~ (~~I and J~~) ~~or CPS49 (K~~ and ~~L). f-actin antibody staining highlights increased stress fibers~~ in ~~CPS49-treated cells~~ (K) ~~but not~~ in ~~DMSO-treated cells~~ (~~I). alpha-tubulin staining indicates a decrease in tubulin structure between~~ DMSO (J) and CPS49 (L) treated ~~cells~~.		('''G and H''') Number of tip cell filopodia (G) and accumulative change in ISV length (H) in control (DMSO; open circles) and CPS49 (filled circles) treated zebrafish embryos imaged by using time-lapse photography.

	(M) Model of CPS49 action on endothelial cell and vessel outgrowth, where new vessel networks are stunted and lost as CPS49 prevents all angiogenesis, but in stable, established, mature networks, only the distal cell is prevented from angiogenesis and stunted temporarily.

	(N) ~~Model~~ of ~~action~~ of CPS49 ~~of the limb highlighting the developmental stage dependence of drug action~~. ~~Treatment at HHst18 results~~ in ~~limb outgrowth failure, as all vessels are unstable and affected, whereas at HHst23, proximal vessels are stable,~~ but ~~distal vessels are angiogenic and lost resulting~~ in ~~loss of the handplate~~. ~~We hypothesize that phocomelia arises because of some FGF signaling remaining, which can then pattern the resulting limb stump~~.		('''I–L''') Confocal images of HUVEC cultured cells after 1 h of treatment with DMSO (I and J) or CPS49 (K and L). f-actin antibody staining highlights increased stress fibers in CPS49-treated cells (K) but not in DMSO-treated cells (I). alpha-tubulin staining indicates a decrease in tubulin structure between DMSO (J) and CPS49 (L) treated cells.

	(Scale bars: A, B, D, and E, 25 μm; C and F, 12.5 μm; I–L, 10 μm.)
			('''M''') Model of CPS49 action on endothelial cell and vessel outgrowth, where new vessel networks are stunted and lost as CPS49 prevents all angiogenesis, but in stable, established, mature networks, only the distal cell is prevented from angiogenesis and stunted temporarily.


			('''N''') Model of action of CPS49 of the limb highlighting the developmental stage dependence of drug action. Treatment at HHst18 results in limb outgrowth failure, as all vessels are unstable and affected, whereas at HHst23, proximal vessels are stable, but distal vessels are angiogenic and lost resulting in loss of the handplate. We hypothesize that phocomelia arises because of some {{FGF}} signaling remaining, which can then pattern the resulting limb stump.


			(Scale bars: A, B, D, and E, 25 μm; C and F, 12.5 μm; I–L, 10 μm; text modified from figure legend)

			:Links: {{Thalidomide}}

	===Reference===		===Reference===
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	Fig. 4. http://www.pnas.org/content/106/21/8573/F4.expansion.html		Fig. 4. http://www.pnas.org/content/106/21/8573/F4.expansion.html

			{{Footer}}
	[[Category:Abnormal Development]] [[Category:Chicken]] [[Category:Limb]] [[Category:Cardiovascular]]		[[Category:Abnormal Development]] [[Category:Chicken]] [[Category:Limb]] [[Category:Cardiovascular]]

Z8600021 at 03:22, 11 April 2019

2019-04-11T03:22:26Z

← Older revision		Revision as of 14:22, 11 April 2019
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	==CPS49 prevents filopodial extensions in endothelial cells ~~<ref><pubmed>19433787</pubmed></ref>~~==		==CPS49 prevents filopodial extensions in endothelial cells==

	CPS49 - a tetrafluorinated analogue of thalidomide that is chemically and structurally related to thalidomide breakdown products.		CPS49 - a tetrafluorinated analogue of thalidomide that is chemically and structurally related to thalidomide breakdown products.
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	(Scale bars: A, B, D, and E, 25 μm; C and F, 12.5 μm; I–L, 10 μm.)		(Scale bars: A, B, D, and E, 25 μm; C and F, 12.5 μm; I–L, 10 μm.)

			===Reference===
			{{#pmid:19433787}}


			{{PNAS}}

	Fig. 4. http://www.pnas.org/content/106/21/8573/F4.expansion.html		Fig. 4. http://www.pnas.org/content/106/21/8573/F4.expansion.html

	~~<references/>~~

	~~{{Template:PNAS}}~~

	[[Category:Abnormal Development]] [[Category:Chicken]] [[Category:Limb]] [[Category:Cardiovascular]]		[[Category:Abnormal Development]] [[Category:Chicken]] [[Category:Limb]] [[Category:Cardiovascular]]

S8600021 at 06:07, 17 Haziran 2010

2010-06-17T06:07:49Z

← Older revision		Revision as of 17:07, 17 Haziran 2010
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	{{Template:PNAS}}		{{Template:PNAS}}

			[[Category:Abnormal Development]] [[Category:Chicken]] [[Category:Limb]] [[Category:Cardiovascular]]

S8600021 at 03:06, 17 Haziran 2010

2010-06-17T03:06:21Z

← Older revision		Revision as of 14:06, 17 Haziran 2010
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	CPS49 prevents filopodial extensions in endothelial cells <ref><pubmed>19433787</pubmed></ref>		==CPS49 prevents filopodial extensions in endothelial cells <ref><pubmed>19433787</pubmed></ref>==

	CPS49 - a tetrafluorinated analogue of thalidomide that is chemically and structurally related to thalidomide breakdown products.		CPS49 - a tetrafluorinated analogue of thalidomide that is chemically and structurally related to thalidomide breakdown products.

S8600021: CPS49 prevents filopodial extensions in endothelial cells 19433787 CPS49 - a tetrafluorinated analogue of thalidomide that is chemically and structurally related to thalidomide breakdown products. (A–F) Confocal images of z

2010-06-17T03:05:59Z

New page

CPS49 prevents filopodial extensions in endothelial cells <ref><pubmed>19433787</pubmed></ref>

CPS49 - a tetrafluorinated analogue of thalidomide that is chemically and structurally related to thalidomide breakdown products.

(A–F) Confocal images of zebrafish intersomitic vessel (ISV) formation 3 h (A–C) or 6 h (D–F) after DMSO (A and D) or CPS49 (B, C, E, and F) treatment at 24 h postfertilization.

Black arrowheads in B and E are shown at higher power in C and F. CPS49 reduced the number of filopodia and filopodial extensions (arrowheads), stunted forming ISVs (black asterisk), and prevented formation of a patent, functional vascular network (black arrows; compare A, B, D, and E).

(G and H) Number of tip cell filopodia (G) and accumulative change in ISV length (H) in control (DMSO; open circles) and CPS49 (filled circles) treated zebrafish embryos imaged by using time-lapse photography.

(I–L) Confocal images of HUVEC cultured cells after 1 h of treatment with DMSO (I and J) or CPS49 (K and L). f-actin antibody staining highlights increased stress fibers in CPS49-treated cells (K) but not in DMSO-treated cells (I). alpha-tubulin staining indicates a decrease in tubulin structure between DMSO (J) and CPS49 (L) treated cells.

(M) Model of CPS49 action on endothelial cell and vessel outgrowth, where new vessel networks are stunted and lost as CPS49 prevents all angiogenesis, but in stable, established, mature networks, only the distal cell is prevented from angiogenesis and stunted temporarily.

(N) Model of action of CPS49 of the limb highlighting the developmental stage dependence of drug action. Treatment at HHst18 results in limb outgrowth failure, as all vessels are unstable and affected, whereas at HHst23, proximal vessels are stable, but distal vessels are angiogenic and lost resulting in loss of the handplate. We hypothesize that phocomelia arises because of some FGF signaling remaining, which can then pattern the resulting limb stump.

(Scale bars: A, B, D, and E, 25 μm; C and F, 12.5 μm; I–L, 10 μm.)

Fig. 4. http://www.pnas.org/content/106/21/8573/F4.expansion.html

<references/>

{{Template:PNAS}}