File:Paternal chromatin mouse embryos.jpg - Revision history

Z8600021 at 00:32, 18 December 2018

2018-12-18T00:32:46Z

← Older revision		Revision as of 11:32, 18 December 2018
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	'''Reference'''		'''Reference'''
			{{#pmid:10684246}}

	~~<pubmed>PMC2169371</pubmed>~~

	* Ownership of copyright in the Work remains with the Authors.		* Ownership of copyright in the Work remains with the Authors.

Z3254758: /* Distribution of paternal chromatin in early mouse embryos */

2012-08-05T02:56:04Z

Distribution of paternal chromatin in early mouse embryos

Z3254758: ==Distribution of paternal chromatin in early mouse embryos== BrdU-treated male mice were mated with untreated females and the resulting embryos stained with FITC-conjugated anti-BrdU antibody (green). Nuclei and chromosomes were counterstained with DAPI

2012-08-05T02:45:38Z

New page

==Distribution of paternal chromatin in early mouse embryos==

BrdU-treated male mice were mated with untreated females and the resulting embryos stained with FITC-conjugated anti-BrdU antibody (green). Nuclei and chromosomes were counterstained with DAPI (blue). a, Highly condensed sperm nucleus and fertilized egg (3). Numbers in parentheses indicate the number of embryos analyzed. b, Male and female pronuclei at 10 h after fertilization (20). The somewhat larger male pronucleus shows a nearly uniform BrdU staining, indicating that the entire sperm DNA is substituted with BrdU. c, After nuclear envelope breakdown the two chromosome sets form a single diploid nucleus (2). d, First metaphase at 20 h after fertilization (5). e, Two-cell embryo during G1 phase at 22 h (>10). The second polar body remains completely BrdU negative. f, Two-cell embryo during G2 phase at 32 h (>10). The male chromatin occupies approximately half of the nuclear volume. g, Four-cell embryo and second polar body at 45 h after fertilization (10). At this point, only half of the paternal chromosomes are still labeled with BrdU. h, 32-cell embryo at 78 h (>5). The one or two BrdU-positive sperm DNA strands per nucleus are consistent with random strand-segregation mechanisms. Bars, 10 μm.

'''Reference'''

M Wolfgang, A Smith, R Fundele, T Haaf. Spatial Separation of Parental Genomes in Preimplantation Mouse Embryos. The Journal of Cell Biology. 2000; 148(4): 629–634. PMID- 2169371

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← Older revision		Revision as of 13:56, 5 August 2012
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	BrdU-treated male mice were mated with untreated females and the resulting embryos stained with FITC-conjugated anti-BrdU antibody (green). Nuclei and chromosomes were counterstained with DAPI (blue). a, Highly condensed sperm nucleus and fertilized egg (3). Numbers in parentheses indicate the number of embryos analyzed. b, Male and female pronuclei at 10 h after fertilization (20). The somewhat larger male pronucleus shows a nearly uniform BrdU staining, indicating that the entire sperm DNA is substituted with BrdU. c, After nuclear envelope breakdown the two chromosome sets form a single diploid nucleus (2). d, First metaphase at 20 h after fertilization (5). e, Two-cell embryo during G1 phase at 22 h (>10). The second polar body remains completely BrdU negative. f, Two-cell embryo during G2 phase at 32 h (>10). The male chromatin occupies approximately half of the nuclear volume. g, Four-cell embryo and second polar body at 45 h after fertilization (10). At this point, only half of the paternal chromosomes are still labeled with BrdU. h, 32-cell embryo at 78 h (>5). The one or two BrdU-positive sperm DNA strands per nucleus are consistent with random strand-segregation mechanisms. Bars, 10 μm.		BrdU-treated male mice were mated with untreated females and the resulting embryos stained with FITC-conjugated anti-BrdU antibody (green). Nuclei and chromosomes were counterstained with DAPI (blue). a, Highly condensed sperm nucleus and fertilized egg (3). Numbers in parentheses indicate the number of embryos analyzed. b, Male and female pronuclei at 10 h after fertilization (20). The somewhat larger male pronucleus shows a nearly uniform BrdU staining, indicating that the entire sperm DNA is substituted with BrdU. c, After nuclear envelope breakdown the two chromosome sets form a single diploid nucleus (2). d, First metaphase at 20 h after fertilization (5). e, Two-cell embryo during G1 phase at 22 h (>10). The second polar body remains completely BrdU negative. f, Two-cell embryo during G2 phase at 32 h (>10). The male chromatin occupies approximately half of the nuclear volume. g, Four-cell embryo and second polar body at 45 h after fertilization (10). At this point, only half of the paternal chromosomes are still labeled with BrdU. h, 32-cell embryo at 78 h (>5). The one or two BrdU-positive sperm DNA strands per nucleus are consistent with random strand-segregation mechanisms. Bars, 10 μm.


	'''Reference'''		'''Reference'''

	~~M Wolfgang, A Smith, R Fundele, T Haaf. Spatial Separation of Parental Genomes in Preimplantation Mouse Embryos. The Journal of Cell Biology. 2000; 148(4): 629–634. PMID- 2169371~~		<pubmed>PMC2169371</pubmed>

	1. Ownership of copyright in the Work remains with the Authors.		* Ownership of copyright in the Work remains with the Authors.

	2. The Authors retain the non-exclusive right to do anything they want with the Work, so long as the Authors provide attribution to the place of original publication. The retained right specifically includes the right to post the Work on the authors’ or their institutions’ web sites.		* The Authors retain the non-exclusive right to do anything they want with the Work, so long as the Authors provide attribution to the place of original publication. The retained right specifically includes the right to post the Work on the authors’ or their institutions’ web sites.

	3. The Authors grant The Rockefeller University Press (RUP) a license (a) to publish, reproduce, distribute, display and store the Work in all forms, (b) to translate the Work into other languages, create adaptations, summaries or extracts of the Work or other derivative works based on the Work and exercise all of the rights set forth in (a) above in such translations, adaptations, summaries, extracts and derivative works, and (c) to license others to do any or all of the above. For the first six months after publication, this license will be exclusive to RUP (except as RUP may grant sublicenses). Beginning six months after publication, the Work will be made freely available to the public on RUP’s website, and RUP’s license will be non-exclusive. The Authors acknowledge that RUP will submit all Works to PubMed Central where they will be released to the public six months after the publication date.		* The Authors grant The Rockefeller University Press (RUP) a license (a) to publish, reproduce, distribute, display and store the Work in all forms, (b) to translate the Work into other languages, create adaptations, summaries or extracts of the Work or other derivative works based on the Work and exercise all of the rights set forth in (a) above in such translations, adaptations, summaries, extracts and derivative works, and (c) to license others to do any or all of the above. For the first six months after publication, this license will be exclusive to RUP (except as RUP may grant sublicenses). Beginning six months after publication, the Work will be made freely available to the public on RUP’s website, and RUP’s license will be non-exclusive. The Authors acknowledge that RUP will submit all Works to PubMed Central where they will be released to the public six months after the publication date.

	4. For the first six months after publication, RUP grants the public the non-exclusive right to copy, distribute, or display the Work, under the following conditions:		* For the first six months after publication, RUP grants the public the non-exclusive right to copy, distribute, or display the Work, under the following conditions:

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	~~The full legal text of the license is available here.~~

	5. Beginning six months after publication, RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode.		* Beginning six months after publication, RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode.

	6. “Work” is defined as the final published article appearing in an RUP journal, including all forms of supplemental material and any images used for cover art or for any other journal feature.		* “Work” is defined as the final published article appearing in an RUP journal, including all forms of supplemental material and any images used for cover art or for any other journal feature.

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