Z8600021 at 00:54, 22 October 2018

2018-10-22T00:54:10Z

← Older revision		Revision as of 11:54, 22 October 2018
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	(A, B) The expression of Bcl11b (A, B), Znf750 (C, D), Jag2 (E,F) and Perp (G, H), all of which are down-regulated in the medial edge epithelia of wild-type mice, is maintained in the medial edge epithelia of their Krt5-tTA;pTRE-ΔNp63α bi-transgenic littermates. In contrast, Mmp13 which is normally expressed in the midline epithelial seam of wild-type mice as they adhere until degeneration of the seam, is down-regulated in the medial edge epithelia of Krt5-tTA;pTRE-ΔNp63α embryos. p: palatal shelves; t: tongue. Scale bars: 200 μm.		(A, B) The expression of Bcl11b (A, B), Znf750 (C, D), Jag2 (E,F) and Perp (G, H), all of which are down-regulated in the medial edge epithelia of wild-type mice, is maintained in the medial edge epithelia of their Krt5-tTA;pTRE-ΔNp63α bi-transgenic littermates. In contrast, Mmp13 which is normally expressed in the midline epithelial seam of wild-type mice as they adhere until degeneration of the seam, is down-regulated in the medial edge epithelia of Krt5-tTA;pTRE-ΔNp63α embryos. p: palatal shelves; t: tongue. Scale bars: 200 μm.

			Cleft palate is a common congenital disorder that affects up to 1 in 2500 live births and results in considerable morbidity to affected individuals and their families. The aetiology of cleft palate is complex with both genetic and environmental factors implicated. Mutations in the transcription factor p63 are one of the major individual causes of cleft palate; however, the gene regulatory networks in which p63 functions remain only partially characterized. Our findings demonstrate that p63 functions as an essential regulatory molecule in the spatio-temporal control of palatal epithelial cell fate to ensure appropriate fusion of the palatal shelves. Initially, p63 induces periderm formation and controls its subsequent maintenance to prevent premature adhesion between adhesion-competent, intra-oral epithelia. Subsequently, TGFβ3-induced down-regulation of p63 in the medial edge epithelia of the palatal shelves is a pre-requisite for palatal fusion by facilitating periderm migration from, and reducing the proliferative potential of, the midline epithelial seam thereby preventing cleft palate.

			===Reference===
			{{#pmid:28604778}}

			====Copyright====
			© 2017 Richardson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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			[[Category:Mouse]][[Category:Apoptosis]][[Category:Palate]][[Category:Cleft Palate]]

Z8600021: Molecular consequences of increasing p63 expression during palatal development (A, B) The expression of Bcl11b (A, B), Znf750 (C, D), Jag2 (E,F) and Perp (G, H), all of which are down-regulated in the medial edge epithelia of wild-type mice, is mainta...

2018-10-22T00:52:32Z

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Molecular consequences of increasing p63 expression during palatal development

(A, B) The expression of Bcl11b (A, B), Znf750 (C, D), Jag2 (E,F) and Perp (G, H), all of which are down-regulated in the medial edge epithelia of wild-type mice, is maintained in the medial edge epithelia of their Krt5-tTA;pTRE-ΔNp63α bi-transgenic littermates. In contrast, Mmp13 which is normally expressed in the midline epithelial seam of wild-type mice as they adhere until degeneration of the seam, is down-regulated in the medial edge epithelia of Krt5-tTA;pTRE-ΔNp63α embryos. p: palatal shelves; t: tongue. Scale bars: 200 μm.

Pgen.1006828.g007.jpg

File:Mouse palate increasing p63.jpg - Revision history

Z8600021 at 00:54, 22 October 2018

Z8600021: Molecular consequences of increasing p63 expression during palatal development (A, B) The expression of Bcl11b (A, B), Znf750 (C, D), Jag2 (E,F) and Perp (G, H), all of which are down-regulated in the medial edge epithelia of wild-type mice, is mainta...