File:Mouse blastocyst movie icon.jpg - Revision history

Z8600021 at 21:35, 9 November 2015

2015-11-09T21:35:35Z

← Older revision		Revision as of 08:35, 10 November 2015
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	This shows embryo development observed using time-lapse microscopy from the pronuclear to blastocyst stages without using a specialized incubation chamber.		This shows embryo development observed using time-lapse microscopy from the pronuclear to blastocyst stages without using a specialized incubation chamber.

	~~===Offspring from mouse embryos developed using a simple incubator-free culture system with a deoxidizing agent===~~

	~~Itoi F, Tokoro M, Terashita Y, Yamagata K, Fukunaga N, Asada Y, Wakayama T.~~

	~~Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN, Kobe, Japan ; The Asada Institute for Reproductive Medicine, Asada Ladies Clinic, Kasugai, Japan.~~

	~~Abstract~~

	To culture preimplantation embryos in vitro, water-jacketed CO(2) incubators are used widely for maintaining an optimal culture environment in terms of gas phase, temperature and humidity. We investigated the possibility of mouse embryo culture in a plastic bag kept at 37°C. Zygotes derived from in vitro fertilization or collected from naturally mated B6D2F1 female mice were put in a drop of medium on a plastic culture dish and then placed in a commercially available plastic bag. When these were placed in an oven under air at 37°C for 96 h, the rate of blastocyst development and the cell numbers of embryos decreased. However, when the concentration of O(2) was reduced to 5% using a deoxidizing agent and a small oxygen meter, most zygotes developed into blastocysts. These blastocysts were judged normal according to their cell number, Oct3/4 and Cdx2 gene expression levels, the apoptosis rate and the potential for full-term development after embryo transfer to pseudopregnant recipients. Furthermore, using this system, normal offspring were obtained simply by keeping the bag on a warming plate. This culture method was applied successfully to both hybrid and inbred strains. In addition, because the developing embryos could be observed through the transparent wall of the bag, it was possible to capture time-lapse images of live embryos until the blastocyst stage without needing an expensive microscope-based incubation chamber. These results suggest that mouse zygotes are more resilient to their environment than generally believed. This method might prove useful in economical culture systems or for the international shipment of embryos.


	{{Mouse blastocyst movie}}		{{Mouse blastocyst movie}}

Z8600021 at 21:35, 9 November 2015

2015-11-09T21:35:24Z

← Older revision		Revision as of 08:35, 10 November 2015
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	<pubmed>23056643</pubmed>\| [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0047512 PLoS ONE]		<pubmed>23056643</pubmed>\| [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0047512 PLoS ONE]

			====Copyright====
			© 2012 Itoi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

	Citation: Itoi F, Tokoro M, Terashita Y, Yamagata K, Fukunaga N, et al. (2012) Offspring from Mouse Embryos Developed Using a Simple Incubator-Free Culture System with a Deoxidizing Agent. PLoS ONE 7(10): e47512. doi:10.1371/journal.pone.0047512		Citation: Itoi F, Tokoro M, Terashita Y, Yamagata K, Fukunaga N, et al. (2012) Offspring from Mouse Embryos Developed Using a Simple Incubator-Free Culture System with a Deoxidizing Agent. PLoS ONE 7(10): e47512. doi:10.1371/journal.pone.0047512

			{{Footer}}

	Copyright: © 2012 Itoi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

	[[Category:Mouse]] [[Category:Zygote]] [[Category:Morula]] [[Category:Blastocyst]]		[[Category:Mouse]] [[Category:Zygote]] [[Category:Morula]] [[Category:Blastocyst]]

Z8600021 at 01:02, 14 October 2012

2012-10-14T01:02:07Z

← Older revision		Revision as of 12:02, 14 October 2012
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	==Mouse ~~blastocyst development~~==		==Mouse Blastocyst Development==

	This shows embryo development observed using time-lapse microscopy from the pronuclear to blastocyst stages without using a specialized incubation chamber.		This shows embryo development observed using time-lapse microscopy from the pronuclear to blastocyst stages without using a specialized incubation chamber.
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			{{Mouse blastocyst movie}}

	===Reference===		===Reference===
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	Copyright: © 2012 Itoi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.		Copyright: © 2012 Itoi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

	[[Category:Mouse]]		[[Category:Mouse]] [[Category:Zygote]] [[Category:Morula]] [[Category:Blastocyst]]

Z8600021 at 00:59, 14 October 2012

2012-10-14T00:59:09Z

← Older revision		Revision as of 11:59, 14 October 2012
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	This shows embryo development observed using time-lapse microscopy from the pronuclear to blastocyst stages without using a specialized incubation chamber.		This shows embryo development observed using time-lapse microscopy from the pronuclear to blastocyst stages without using a specialized incubation chamber.

			===Offspring from mouse embryos developed using a simple incubator-free culture system with a deoxidizing agent===

			Itoi F, Tokoro M, Terashita Y, Yamagata K, Fukunaga N, Asada Y, Wakayama T.

			Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN, Kobe, Japan ; The Asada Institute for Reproductive Medicine, Asada Ladies Clinic, Kasugai, Japan.

			Abstract

			To culture preimplantation embryos in vitro, water-jacketed CO(2) incubators are used widely for maintaining an optimal culture environment in terms of gas phase, temperature and humidity. We investigated the possibility of mouse embryo culture in a plastic bag kept at 37°C. Zygotes derived from in vitro fertilization or collected from naturally mated B6D2F1 female mice were put in a drop of medium on a plastic culture dish and then placed in a commercially available plastic bag. When these were placed in an oven under air at 37°C for 96 h, the rate of blastocyst development and the cell numbers of embryos decreased. However, when the concentration of O(2) was reduced to 5% using a deoxidizing agent and a small oxygen meter, most zygotes developed into blastocysts. These blastocysts were judged normal according to their cell number, Oct3/4 and Cdx2 gene expression levels, the apoptosis rate and the potential for full-term development after embryo transfer to pseudopregnant recipients. Furthermore, using this system, normal offspring were obtained simply by keeping the bag on a warming plate. This culture method was applied successfully to both hybrid and inbred strains. In addition, because the developing embryos could be observed through the transparent wall of the bag, it was possible to capture time-lapse images of live embryos until the blastocyst stage without needing an expensive microscope-based incubation chamber. These results suggest that mouse zygotes are more resilient to their environment than generally believed. This method might prove useful in economical culture systems or for the international shipment of embryos.

Z8600021: /* Reference */

2012-10-14T00:00:42Z

Reference

← Older revision		Revision as of 11:00, 14 October 2012
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	===Reference===		===Reference===
	<pubmed>23056643</pubmed> \| [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0047512 PLoS ONE]		<pubmed>23056643</pubmed>\| [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0047512 PLoS ONE]

Z8600021: ==Mouse blastocyst development== This shows embryo development observed using time-lapse microscopy from the pronuclear to blastocyst stages without using a specialized incubation chamber. ===Reference=== 23056643 | [http://www.plosone

2012-10-13T23:57:03Z

New page

==Mouse blastocyst development==

This shows embryo development observed using time-lapse microscopy from the pronuclear to blastocyst stages without using a specialized incubation chamber.

===Reference===
<pubmed>23056643</pubmed> | [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0047512 PLoS ONE]

Citation: Itoi F, Tokoro M, Terashita Y, Yamagata K, Fukunaga N, et al. (2012) Offspring from Mouse Embryos Developed Using a Simple Incubator-Free Culture System with a Deoxidizing Agent. PLoS ONE 7(10): e47512. doi:10.1371/journal.pone.0047512

Copyright: © 2012 Itoi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

[[Category:Mouse]]