File:Mouse- blastocyst GLUT8 expression.jpg - Revision history

S8600021: /* Abstract */

2010-12-02T23:16:42Z

Abstract

← Older revision		Revision as of 10:16, 3 December 2010
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	Mammalian preimplantation blastocysts exhibit insulin-stimulated glucose uptake despite the absence of the only known insulin-regulated transporter, GLUT4. We describe a previously unidentified member of the mammalian facilitative GLUT superfamily that exhibits approximately 20-25% identity with other murine facilitative GLUTs. Insulin induces a change in the intracellular localization of this protein, which translates into increased glucose uptake into the blastocyst, a process that is inhibited by antisense oligoprobes. Presence of this transporter may be necessary for successful blastocyst development, fuel metabolism, and subsequent implantation. Moreover, the existence of an alternative transporter may explain examples in other tissues of insulin-regulated glucose transport in the absence of GLUT4.		Mammalian preimplantation blastocysts exhibit insulin-stimulated glucose uptake despite the absence of the only known insulin-regulated transporter, GLUT4. We describe a previously unidentified member of the mammalian facilitative GLUT superfamily that exhibits approximately 20-25% identity with other murine facilitative GLUTs. Insulin induces a change in the intracellular localization of this protein, which translates into increased glucose uptake into the blastocyst, a process that is inhibited by antisense oligoprobes. Presence of this transporter may be necessary for successful blastocyst development, fuel metabolism, and subsequent implantation. Moreover, the existence of an alternative transporter may explain examples in other tissues of insulin-regulated glucose transport in the absence of GLUT4.

			* GLUT8 is a glucose transporter responsible for insulin-stimulated glucose uptake in the blastocyst.
	* GLUT8 is a glucose transporter responsible for insulin-stimulated glucose uptake in the blastocyst
	* mammalian development when embryonic fuel metabolism switches from the oxidation of lactate and pyruvate via the Krebs cycle and oxidative phosphorylation to anaerobic metabolism of glucose via glycolysis.		* mammalian development when embryonic fuel metabolism switches from the oxidation of lactate and pyruvate via the Krebs cycle and oxidative phosphorylation to anaerobic metabolism of glucose via glycolysis.
	* This critical substrate change is thought to be due to the biosynthetic and developmental demands placed on the embryo as the blastocyst creates the fluid-filled blastocoel and prepares for implantation.		* This critical substrate change is thought to be due to the biosynthetic and developmental demands placed on the embryo as the blastocyst creates the fluid-filled blastocoel and prepares for implantation.

S8600021: /* Mouse Blastocyst GLUT8 Basal and Insulin-Stimulated Expression Expression */

2010-12-02T23:15:27Z

Mouse Blastocyst GLUT8 Basal and Insulin-Stimulated Expression Expression

← Older revision		Revision as of 10:15, 3 December 2010
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	Original file name: Figure 4 http://www.pnas.org/content/97/13/7313/F4.large.jpg		Original file name: Figure 4 http://www.pnas.org/content/97/13/7313/F4.large.jpg (original full figure cropped, relabeled and size adjusted)

	==Reference==		==Reference==

S8600021: ==Mouse Blastocyst GLUT8 Basal and Insulin-Stimulated Expression Expression== Confocal immunofluorescent labeling to localize GLUT8 protein expression in the blastocyst under basal and insulin-stimulated conditions. Blastocyst under basal conditions st

2010-12-02T23:14:43Z

New page

==Mouse Blastocyst GLUT8 Basal and Insulin-Stimulated Expression Expression==

Confocal immunofluorescent labeling to localize GLUT8 protein expression in the blastocyst under basal and insulin-stimulated conditions.

Blastocyst under basal conditions stained with peptide-purified polyclonal antibody to the C terminus of GLUT8 (green) and propidium iodide (red) as a nuclear stain. This technique localizes the protein to the trophectoderm and primitive endoderm lining the blastocoel. Higher magnification of the trophectoderm revealing GLUT8 in vesicle-like intracellular compartment.

Blastocyst under insulin-stimulated conditions stained with the same GLUT8 antibody. This technique demonstrates a general cellular redistribution of the protein with increased plasma membrane staining. Higher magnification of the blastocyst cells revealing cytoplasmic and plasma membrane staining and less staining of the vesicle-like compartments.

Original file name: Figure 4 http://www.pnas.org/content/97/13/7313/F4.large.jpg

==Reference==
<pubmed>10860996</pubmed>| [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC16542 PMC16542] | [http://www.pnas.org/content/97/13/7313.full Proc Natl Acad Sci U S A.]

===Abstract===

Mammalian preimplantation blastocysts exhibit insulin-stimulated glucose uptake despite the absence of the only known insulin-regulated transporter, GLUT4. We describe a previously unidentified member of the mammalian facilitative GLUT superfamily that exhibits approximately 20-25% identity with other murine facilitative GLUTs. Insulin induces a change in the intracellular localization of this protein, which translates into increased glucose uptake into the blastocyst, a process that is inhibited by antisense oligoprobes. Presence of this transporter may be necessary for successful blastocyst development, fuel metabolism, and subsequent implantation. Moreover, the existence of an alternative transporter may explain examples in other tissues of insulin-regulated glucose transport in the absence of GLUT4.

* GLUT8 is a glucose transporter responsible for insulin-stimulated glucose uptake in the blastocyst
* mammalian development when embryonic fuel metabolism switches from the oxidation of lactate and pyruvate via the Krebs cycle and oxidative phosphorylation to anaerobic metabolism of glucose via glycolysis.
* This critical substrate change is thought to be due to the biosynthetic and developmental demands placed on the embryo as the blastocyst creates the fluid-filled blastocoel and prepares for implantation.

{{Template:PNAS}}

[[Category:Mouse]] [[Category:Blastocyst]] [[Category:Fluorescence]]