File:Model GATA6 hindlimb.jpg - Revision history

Z8600021: /* Model for GATA6 action Patterning developing Hindlimb Buds */

2020-08-24T12:50:36Z

Model for GATA6 action Patterning developing Hindlimb Buds

← Older revision		Revision as of 23:50, 24 August 2020
Line 1:		Line 1:
	==Model for GATA6 action Patterning developing Hindlimb Buds==		==Model for GATA6 action Patterning developing Hindlimb Buds==

	GATA6 is expressed in the anterior portion of the developing mouse hindlimb bud (displayed in top figures), and specifically represses polydactyly (PD) in the hindlimb by directly blocking anterior expression of {{Shh}} (most likely in a FOG-dependent fashion), and thus represses ectopic expression of both direct Shh transcriptional targets (Gli1 and Grem1) in anterior limb bud mesenchyme and indirect targets ({{FGF}}4/8) in the anterior AER. In hindlimb buds engineered to lack GATA6 (displayed in bottom figures), ectopic expression of Shh in the anterior mesenchyme induces expression of both Gli2/3 FL and Gli1 which activate expression of the {{BMP}} antagonist Grem1, which in turn derepresses expression of FGF4/8 in the anterior region of the apical ectodermal ridge. Thus, as a consequence of deletion of GATA6 in Prx1-Cre; GATA6fl/fl animals, the hindlimbs of these animals display preaxial polydactyly. Expressed genes are indicated by black font; knocked-out or repressed genes are indicated by grey font.		GATA6 is expressed in the anterior portion of the developing {{mouse}} hindlimb bud (displayed in top figures), and specifically represses polydactyly (PD) in the hindlimb by directly blocking anterior expression of {{Shh}} (most likely in a FOG-dependent fashion), and thus represses ectopic expression of both direct Shh transcriptional targets (Gli1 and Grem1) in anterior limb bud mesenchyme and indirect targets ({{FGF}}4/8) in the anterior AER. In hindlimb buds engineered to lack GATA6 (displayed in bottom figures), ectopic expression of Shh in the anterior mesenchyme induces expression of both Gli2/3 FL and Gli1 which activate expression of the {{BMP}} antagonist Grem1, which in turn derepresses expression of FGF4/8 in the anterior region of the apical ectodermal ridge. Thus, as a consequence of deletion of GATA6 in Prx1-Cre; GATA6fl/fl animals, the hindlimbs of these animals display preaxial polydactyly. Expressed genes are indicated by black font; knocked-out or repressed genes are indicated by grey font.

Z8600021: /* Model for GATA6 action Patterning developing Hindlimb Buds */

2020-08-24T12:49:01Z

Model for GATA6 action Patterning developing Hindlimb Buds

← Older revision		Revision as of 23:49, 24 August 2020
Line 1:		Line 1:
	==Model for GATA6 action Patterning developing Hindlimb Buds==		==Model for GATA6 action Patterning developing Hindlimb Buds==

	GATA6 is expressed in the anterior portion of the developing mouse hindlimb bud (displayed in top figures), and specifically represses polydactyly (PD) in the hindlimb by directly blocking anterior expression of Shh (most likely in a FOG-dependent fashion), and thus represses ectopic expression of both direct Shh transcriptional targets (Gli1 and Grem1) in anterior limb bud mesenchyme and indirect targets (~~FGF4~~/8) in the anterior AER. In hindlimb buds engineered to lack GATA6 (displayed in bottom figures), ectopic expression of Shh in the anterior mesenchyme induces expression of both Gli2/3 FL and Gli1 which activate expression of the BMP antagonist Grem1, which in turn derepresses expression of FGF4/8 in the anterior region of the apical ectodermal ridge. Thus, as a consequence of deletion of GATA6 in Prx1-Cre; GATA6fl/fl animals, the hindlimbs of these animals display preaxial polydactyly. Expressed genes are indicated by black font; knocked-out or repressed genes are indicated by grey font.		GATA6 is expressed in the anterior portion of the developing mouse hindlimb bud (displayed in top figures), and specifically represses polydactyly (PD) in the hindlimb by directly blocking anterior expression of {{Shh}} (most likely in a FOG-dependent fashion), and thus represses ectopic expression of both direct Shh transcriptional targets (Gli1 and Grem1) in anterior limb bud mesenchyme and indirect targets ({{FGF}}4/8) in the anterior AER. In hindlimb buds engineered to lack GATA6 (displayed in bottom figures), ectopic expression of Shh in the anterior mesenchyme induces expression of both Gli2/3 FL and Gli1 which activate expression of the {{BMP}} antagonist Grem1, which in turn derepresses expression of FGF4/8 in the anterior region of the apical ectodermal ridge. Thus, as a consequence of deletion of GATA6 in Prx1-Cre; GATA6fl/fl animals, the hindlimbs of these animals display preaxial polydactyly. Expressed genes are indicated by black font; knocked-out or repressed genes are indicated by grey font.


	===Reference===		===Reference===
Line 13:		Line 14:

	{{footer}}		{{footer}}

	[[Category:Limb]][[Category:Mouse]][[Category:Sonic Hedgehog]][[Category:2014]]		[[Category:Limb]][[Category:Mouse]][[Category:Sonic Hedgehog]][[Category:2014]]

Z8600021: /* Reference */

2020-08-24T12:47:49Z

Reference

← Older revision		Revision as of 23:47, 24 August 2020
Line 4:		Line 4:

	===Reference===		===Reference===
	~~<pubmed>~~24415953~~</pubmed>\| [http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1004072 PLoS Genet.]~~		{{#pmid:24415953}}

	====Copyright====		====Copyright====
Line 11:		Line 11:

	Figure 7. doi:10.1371/journal.pgen.1004072.g007 Journal.pgen.1004072.g007.png		Figure 7. doi:10.1371/journal.pgen.1004072.g007 Journal.pgen.1004072.g007.png

			{{footer}}

	[[Category:Limb]][[Category:Mouse]][[Category:Sonic Hedgehog]][[Category:2014]]		[[Category:Limb]][[Category:Mouse]][[Category:Sonic Hedgehog]][[Category:2014]]

Z8600021 at 03:37, 20 Ocak 2014

2014-01-20T03:37:52Z

← Older revision		Revision as of 14:37, 20 Ocak 2014
Line 12:		Line 12:
	Figure 7. doi:10.1371/journal.pgen.1004072.g007 Journal.pgen.1004072.g007.png		Figure 7. doi:10.1371/journal.pgen.1004072.g007 Journal.pgen.1004072.g007.png

	[[Category:Limb]][[Category:Mouse]][[Category:~~SHH~~]][[Category:2014]]		[[Category:Limb]][[Category:Mouse]][[Category:Sonic Hedgehog]][[Category:2014]]

Z8600021 at 03:37, 20 Ocak 2014

2014-01-20T03:37:21Z

← Older revision		Revision as of 14:37, 20 Ocak 2014
Line 12:		Line 12:
	Figure 7. doi:10.1371/journal.pgen.1004072.g007 Journal.pgen.1004072.g007.png		Figure 7. doi:10.1371/journal.pgen.1004072.g007 Journal.pgen.1004072.g007.png

	[[Category:Limb]][[Category:Mouse]][[Category:~~Sonic hedgehog~~]][[Category:2014]]		[[Category:Limb]][[Category:Mouse]][[Category:SHH]][[Category:2014]]

Z8600021 at 03:36, 20 Ocak 2014

2014-01-20T03:36:58Z

← Older revision		Revision as of 14:36, 20 Ocak 2014
Line 4:		Line 4:

	===Reference===		===Reference===
			<pubmed>24415953</pubmed>\| [http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1004072 PLoS Genet.]

			====Copyright====

			© 2014 Kozhemyakina et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

	Figure 7. doi:10.1371/journal.pgen.1004072.g007 Journal.pgen.1004072.g007.png		Figure 7. doi:10.1371/journal.pgen.1004072.g007 Journal.pgen.1004072.g007.png

			[[Category:Limb]][[Category:Mouse]][[Category:Sonic hedgehog]][[Category:2014]]

Z8600021: ==Model for GATA6 action Patterning developing Hindlimb Buds== GATA6 is expressed in the anterior portion of the developing mouse hindlimb bud (displayed in top figures), and specifically represses polydactyly (PD) in the hindlimb by directly blocking...

2014-01-20T03:34:02Z

New page

==Model for GATA6 action Patterning developing Hindlimb Buds==

GATA6 is expressed in the anterior portion of the developing mouse hindlimb bud (displayed in top figures), and specifically represses polydactyly (PD) in the hindlimb by directly blocking anterior expression of Shh (most likely in a FOG-dependent fashion), and thus represses ectopic expression of both direct Shh transcriptional targets (Gli1 and Grem1) in anterior limb bud mesenchyme and indirect targets (FGF4/8) in the anterior AER. In hindlimb buds engineered to lack GATA6 (displayed in bottom figures), ectopic expression of Shh in the anterior mesenchyme induces expression of both Gli2/3 FL and Gli1 which activate expression of the BMP antagonist Grem1, which in turn derepresses expression of FGF4/8 in the anterior region of the apical ectodermal ridge. Thus, as a consequence of deletion of GATA6 in Prx1-Cre; GATA6fl/fl animals, the hindlimbs of these animals display preaxial polydactyly. Expressed genes are indicated by black font; knocked-out or repressed genes are indicated by grey font.

===Reference===

Figure 7. doi:10.1371/journal.pgen.1004072.g007 Journal.pgen.1004072.g007.png