File:FMR1 is silenced in FXS.jpg - Revision history

S8600021: /* Assessment */

2011-10-28T05:49:36Z

Assessment

← Older revision		Revision as of 16:49, 28 October 2011
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	+ This figure is very useful for showing molecular analysis.		+ This figure is very useful for showing molecular analysis.
	- You provide no further information in the figure legend, other than the original information, not fully meeting the peer teaching component.		- You provide no further information in the figure legend, other than the original information, not fully meeting the peer teaching component.
			- You needed to include a list of all acronyms with full details of what they represent.


	{{Template:2011 Student Image}}		{{Template:2011 Student Image}}

S8600021 at 05:47, 28 October 2011

2011-10-28T05:47:52Z

← Older revision		Revision as of 16:47, 28 October 2011
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	'''Reference:''' <pubmed> 18213394</pubmed>\| [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194623 PMC2194623]		'''Reference:''' <pubmed> 18213394</pubmed>\| [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194623 PMC2194623]


			===Assessment===
			+ Figure relavent to group project.
			+ Copyright, citation and student disclaimer included.
			+ This figure is very useful for showing molecular analysis.
			- You provide no further information in the figure legend, other than the original information, not fully meeting the peer teaching component.


	{{Template:2011 Student Image}}		{{Template:2011 Student Image}}

Z3290815: /* FMR1 is silenced in FXS */

2011-10-06T01:18:25Z

FMR1 is silenced in FXS

← Older revision		Revision as of 12:18, 6 October 2011
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	Original file name: Figure 3 Pone.0001486.g003.jpg		Original file name: Figure 3 Pone.0001486.g003.jpg

	~~===Reference===~~		'''Copyright:''' copyright Khalil et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

	<pubmed> 18213394</pubmed>\| [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194623 PMC2194623]		'''Reference:''' <pubmed> 18213394</pubmed>\| [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194623 PMC2194623]


	Copyright Khalil et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


	{{Template:2011 Student Image}}		{{Template:2011 Student Image}}

Z3290808 at 01:37, 22 September 2011

2011-09-22T01:37:48Z

← Older revision		Revision as of 12:37, 22 September 2011
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	==FMR1 is silenced in FXS==		==FMR1 is silenced in FXS==
	--[[User:S8600021\|Mark Hill]] 15:00, 20 September 2011 (EST) I have reformatted your reference, in future please use a similar format. Is this FMR1 or FMR4, your image title differs from the legend description.

			Note that in fragile x syndrome, the effects of FMR1 are equivalent to that of FMR4; ie both are silenced in this disorder.

	FMR4 is silenced in fragile X syndrome.		FMR4 is silenced in fragile X syndrome.

S8600021: /* FMR1 is silenced in FXS */

2011-09-20T05:02:00Z

FMR1 is silenced in FXS

← Older revision		Revision as of 16:02, 20 September 2011
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	==FMR1 is silenced in FXS==		==FMR1 is silenced in FXS==
	--[[User:S8600021\|Mark Hill]] 15:00, 20 September 2011 (EST) I have reformatted your reference, in future please use a similar format. Is this FMR1 or FMR4, your image title ~~edifiers~~ from the legend description.		--[[User:S8600021\|Mark Hill]] 15:00, 20 September 2011 (EST) I have reformatted your reference, in future please use a similar format. Is this FMR1 or FMR4, your image title differs from the legend description.

S8600021: /* FMR1 is silenced in FXS */

2011-09-20T05:01:30Z

FMR1 is silenced in FXS

← Older revision		Revision as of 16:01, 20 September 2011
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	FMR4 is silenced in fragile X syndrome. (A) RNA from four normal, four premutation and four full mutation FXS patients isolated from untransformed leucocytes (kindly provided by Flora Tassone and Paul Hagerman, UC Davis) was reverse transcribed using random hexamers. Quantitative RT-PCR analysis revealed that FMR4, similar to FMR1, is up-regulated in pre-mutation carriers and shut down in full mutation fragile X patients (P<0.0001). (B) RNA from untransformed leucocytes were reversed transcribed and the cDNA was used for PCR analysis. FMR4 is expressed in normal and premutation carriers but no bands were observed in the full mutation fragile X patients (35 cycles). FMR1 bands were observed in normal, premutation, and one of the full mutation patients (35 cycles). To account for any possible DNA contamination, no reverse transcriptase control for all samples were used in the PCR (lanes next to bands are all negative indicating no DNA contamination was present).		FMR4 is silenced in fragile X syndrome.

			(A) RNA from four normal, four premutation and four full mutation FXS patients isolated from untransformed leucocytes (kindly provided by Flora Tassone and Paul Hagerman, UC Davis) was reverse transcribed using random hexamers. Quantitative RT-PCR analysis revealed that FMR4, similar to FMR1, is up-regulated in pre-mutation carriers and shut down in full mutation fragile X patients (P<0.0001).

			(B) RNA from untransformed leucocytes were reversed transcribed and the cDNA was used for PCR analysis. FMR4 is expressed in normal and premutation carriers but no bands were observed in the full mutation fragile X patients (35 cycles). FMR1 bands were observed in normal, premutation, and one of the full mutation patients (35 cycles). To account for any possible DNA contamination, no reverse transcriptase control for all samples were used in the PCR (lanes next to bands are all negative indicating no DNA contamination was present).

	Error bars: s.d.		Error bars: s.d.

S8600021: /* FMR1 is silenced in FXS */

2011-09-20T05:01:10Z

FMR1 is silenced in FXS

← Older revision		Revision as of 16:01, 20 September 2011
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	FMR4 is silenced in fragile X syndrome. (A) RNA from four normal, four premutation and four full mutation FXS patients isolated from untransformed leucocytes (kindly provided by Flora Tassone and Paul Hagerman, UC Davis) was reverse transcribed using random hexamers. Quantitative RT-PCR analysis revealed that FMR4, similar to FMR1, is up-regulated in pre-mutation carriers and shut down in full mutation fragile X patients (P<0.0001). (B) RNA from untransformed leucocytes were reversed transcribed and the cDNA was used for PCR analysis. FMR4 is expressed in normal and premutation carriers but no bands were observed in the full mutation fragile X patients (35 cycles). FMR1 bands were observed in normal, premutation, and one of the full mutation patients (35 cycles). To account for any possible DNA contamination, no reverse transcriptase control for all samples were used in the PCR (lanes next to bands are all negative indicating no DNA contamination was present). Error bars: s.d.		FMR4 is silenced in fragile X syndrome. (A) RNA from four normal, four premutation and four full mutation FXS patients isolated from untransformed leucocytes (kindly provided by Flora Tassone and Paul Hagerman, UC Davis) was reverse transcribed using random hexamers. Quantitative RT-PCR analysis revealed that FMR4, similar to FMR1, is up-regulated in pre-mutation carriers and shut down in full mutation fragile X patients (P<0.0001). (B) RNA from untransformed leucocytes were reversed transcribed and the cDNA was used for PCR analysis. FMR4 is expressed in normal and premutation carriers but no bands were observed in the full mutation fragile X patients (35 cycles). FMR1 bands were observed in normal, premutation, and one of the full mutation patients (35 cycles). To account for any possible DNA contamination, no reverse transcriptase control for all samples were used in the PCR (lanes next to bands are all negative indicating no DNA contamination was present).

			Error bars: s.d.

	Original file name: Figure 3 Pone.0001486.g003.jpg		Original file name: Figure 3 Pone.0001486.g003.jpg
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	===Reference===		===Reference===

	<pubmed> 18213394</pubmed> [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194623 PMC2194623]		<pubmed> 18213394</pubmed>\| [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194623 PMC2194623]

S8600021 at 05:00, 20 September 2011

2011-09-20T05:00:12Z

← Older revision		Revision as of 16:00, 20 September 2011
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	~~Pone~~.~~0001486~~.~~g003.jpg~~		==FMR1 is silenced in FXS==
			--[[User:S8600021\|Mark Hill]] 15:00, 20 September 2011 (EST) I have reformatted your reference, in future please use a similar format. Is this FMR1 or FMR4, your image title edifiers from the legend description.

	Figure 3 FMR4 is silenced in fragile X syndrome. (A) RNA from four normal, four premutation and four full mutation FXS patients isolated from untransformed leucocytes (kindly provided by Flora Tassone and Paul Hagerman, UC Davis) was reverse transcribed using random hexamers. Quantitative RT-PCR analysis revealed that FMR4, similar to FMR1, is up-regulated in pre-mutation carriers and shut down in full mutation fragile X patients (P<0.0001). (B) RNA from untransformed leucocytes were reversed transcribed and the cDNA was used for PCR analysis. FMR4 is expressed in normal and premutation carriers but no bands were observed in the full mutation fragile X patients (35 cycles). FMR1 bands were observed in normal, premutation, and one of the full mutation patients (35 cycles). To account for any possible DNA contamination, no reverse transcriptase control for all samples were used in the PCR (lanes next to bands are all negative indicating no DNA contamination was present). Error bars: s.d.

	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194623/		FMR4 is silenced in fragile X syndrome. (A) RNA from four normal, four premutation and four full mutation FXS patients isolated from untransformed leucocytes (kindly provided by Flora Tassone and Paul Hagerman, UC Davis) was reverse transcribed using random hexamers. Quantitative RT-PCR analysis revealed that FMR4, similar to FMR1, is up-regulated in pre-mutation carriers and shut down in full mutation fragile X patients (P<0.0001). (B) RNA from untransformed leucocytes were reversed transcribed and the cDNA was used for PCR analysis. FMR4 is expressed in normal and premutation carriers but no bands were observed in the full mutation fragile X patients (35 cycles). FMR1 bands were observed in normal, premutation, and one of the full mutation patients (35 cycles). To account for any possible DNA contamination, no reverse transcriptase control for all samples were used in the PCR (lanes next to bands are all negative indicating no DNA contamination was present). Error bars: s.d.

			Original file name: Figure 3 Pone.0001486.g003.jpg

			===Reference===

			<pubmed> 18213394</pubmed> [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194623 PMC2194623]


	Copyright Khalil et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.		Copyright Khalil et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
	~~<ref><pubmed> 18213394</pubmed></ref>~~

	{{Template:2011 Student Image}}		{{Template:2011 Student Image}}

Z3290808: Pone.0001486.g003.jpg Figure 3 FMR4 is silenced in fragile X syndrome. (A) RNA from four normal, four premutation and four full mutation FXS patients isolated from untransformed leucocytes (kindly provided by Flora Tassone and Paul Hagerman, UC Davis) wa

2011-09-17T14:05:29Z

New page

Pone.0001486.g003.jpg

Figure 3 FMR4 is silenced in fragile X syndrome. (A) RNA from four normal, four premutation and four full mutation FXS patients isolated from untransformed leucocytes (kindly provided by Flora Tassone and Paul Hagerman, UC Davis) was reverse transcribed using random hexamers. Quantitative RT-PCR analysis revealed that FMR4, similar to FMR1, is up-regulated in pre-mutation carriers and shut down in full mutation fragile X patients (P<0.0001). (B) RNA from untransformed leucocytes were reversed transcribed and the cDNA was used for PCR analysis. FMR4 is expressed in normal and premutation carriers but no bands were observed in the full mutation fragile X patients (35 cycles). FMR1 bands were observed in normal, premutation, and one of the full mutation patients (35 cycles). To account for any possible DNA contamination, no reverse transcriptase control for all samples were used in the PCR (lanes next to bands are all negative indicating no DNA contamination was present). Error bars: s.d.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2194623/

Copyright Khalil et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
<ref><pubmed> 18213394</pubmed></ref>

{{Template:2011 Student Image}}