Z8600021 at 02:22, 11 October 2012

2012-10-11T02:22:48Z

← Older revision		Revision as of 13:22, 11 October 2012
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	==Detached-cell ~~method~~ of ~~transplanting nuclei~~==		==Detached-cell Method of Transplanting Muclei==

	* A - A single donor cell is sucked into a ~~micropipette~~.		This figure from a classic J Embryol Exp Morphol. (now Development) paper shows the technique for transplanting a single nuclei from monolayers of cultured cells to unfertilized eggs of Xenopus laevis.
	* B - The air bubble marker moves sufficiently far up the ~~pipette shaft~~ to ~~serve as a guide when the pipette tip and donor cell have been immersed in the recipient egg~~.

	~~Attached~~-~~cell method~~ of ~~transplanting~~ nuclei.		One of the authors, [[Embryology_History_-_John_Gurdon\|John Gurdon]], was awarded the 2012 Nobel prize in Medicine for studies that involved this technique of nuclei transplantation.

	C, D - The edges of a well-spread cell are cut with a fine glass needle.		===Legend===
	E, F - The cytoplasm spreads evenly round the nucleus, and sticks to the glass needle.		* '''A''' - A single donor cell is sucked into a micropipette.
	G, H - The cell (with an incomplete cell membrane) is sucked from the needle into a wide-bore micropipette, and is then injected into a recipient egg in the usual way.		* '''B''' - The air bubble marker moves sufficiently far up the pipette shaft to serve as a guide when the pipette tip and donor cell have been immersed in the recipient egg.
			* '''C, D''' - The edges of a well-spread cell are cut with a fine glass needle.
			* '''E, F''' - The cytoplasm spreads evenly round the nucleus, and sticks to the glass needle.
			* '''G, H''' - The cell (with an incomplete cell membrane) is sucked from the needle into a wide-bore micropipette, and is then injected into a recipient egg in the usual way.

	===Reference===		===Reference===
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	http://dev.biologists.org/site/misc/rights_permissions.xhtml		http://dev.biologists.org/site/misc/rights_permissions.xhtml

	Fig. 1 for educational use only		Fig. 1 original PDF image cropped, resized and labelled with reference.

			for educational use only

			[[Category:Frog]]

Z8600021: ==Detached-cell method of transplanting nuclei== * A - A single donor cell is sucked into a micropipette. * B - The air bubble marker moves sufficiently far up the pipette shaft to serve as a guide when the pipette tip and donor cell have been immersed

2012-10-11T02:17:04Z

New page

==Detached-cell method of transplanting nuclei==

* A - A single donor cell is sucked into a micropipette.
* B - The air bubble marker moves sufficiently far up the pipette shaft to serve as a guide when the pipette tip and donor cell have been immersed in the recipient egg.

Attached-cell method of transplanting nuclei.

C, D - The edges of a well-spread cell are cut with a fine glass needle.
E, F - The cytoplasm spreads evenly round the nucleus, and sticks to the glass needle.
G, H - The cell (with an incomplete cell membrane) is sucked from the needle into a wide-bore micropipette, and is then injected into a recipient egg in the usual way.

===Reference===

<pubmed>4923666</pubmed>| [http://dev.biologists.org/content/24/2/249.long Development] | [http://dev.biologists.org/content/24/2/249.full.pdf PDF]

http://dev.biologists.org/site/misc/rights_permissions.xhtml

Fig. 1 for educational use only

File:Detached-cell method of transplanting nuclei.jpg - Revision history

Z8600021 at 02:22, 11 October 2012

Z8600021: ==Detached-cell method of transplanting nuclei== * A - A single donor cell is sucked into a micropipette. * B - The air bubble marker moves sufficiently far up the pipette shaft to serve as a guide when the pipette tip and donor cell have been immersed