Cell Mitosis Movie - Revision history

Z8600021 at 01:36, 1 September 2014

2014-09-01T01:36:55Z

← Older revision		Revision as of 12:36, 1 September 2014
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	Final part of movie shows the two cells in bright field illumination.		Final part of movie shows the two cells in bright field illumination.




	{{Mitosis movie 1 links}}		{{Mitosis movie 1 links}}
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	A time-lapse video sequence of an EGFP-topoII-expressing GT2-LPk cell imaged from late prometaphase through telophase. Chromosomes align at the metaphase plate with EGFP-topoII concentrated at kinetochores and along the axes of the chromosome arms. As chromosomes separate at anaphase, the kinetochore concentration disappears. In late anaphase, the levels of EGFP-topo II rise inthe cytoplasm, with some concentrated into foci that are motile and, at telophase, appear to fuse with the reforming nuclei. A phase contrast image of the two daughter cells is shown at the end of the sequence. The fluorescence images in this sequence were individually scaled in order to optimize visualization of the EGFP-topoII cytoplasmic foci that appear inanaphase and telophase.		A time-lapse video sequence of an EGFP-topoII-expressing GT2-LPk cell imaged from late prometaphase through telophase. Chromosomes align at the metaphase plate with EGFP-topoII concentrated at kinetochores and along the axes of the chromosome arms. As chromosomes separate at anaphase, the kinetochore concentration disappears. In late anaphase, the levels of EGFP-topo II rise inthe cytoplasm, with some concentrated into foci that are motile and, at telophase, appear to fuse with the reforming nuclei. A phase contrast image of the two daughter cells is shown at the end of the sequence. The fluorescence images in this sequence were individually scaled in order to optimize visualization of the EGFP-topoII cytoplasmic foci that appear inanaphase and telophase.

			GT2-LPk cell derived from LLC-Pk cell line, a pig kidney cell strain.

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	[[Category:Movies]] [[Category:Mitosis]]		[[Category:Movies]] [[Category:Mitosis]] [[Category:Pig]]

	{{Movie footer}}		{{Movie footer}}

Z8600021 at 06:40, 11 August 2014

2014-08-11T06:40:00Z

← Older revision		Revision as of 17:40, 11 August 2014
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	{{Movie header}}		{{Movie header}}
	{\|		{\|
	\| width=420px\|<html5media height="~~420~~" width="~~400~~">File:Mitosis 01.mp4</html5media>		\| width=420px\|<html5media height="520" width="500">File:Mitosis 11.mp4</html5media>

	[[Media:Mitosis 01.mp4\|'''Click Here''' to play on mobile device]]		[[Media:Mitosis 01.mp4\|'''Click Here''' to play on mobile device]]
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	{{Mitosis movie 1 links}}		{{Mitosis movie 1 links}}
			\|-
			\| width=420px\|<html5media height="420" width="400">File:Mitosis 01.mp4</html5media>
	\|}		\|}

Z8600021 at 05:37, 31 July 2014

2014-07-31T05:37:16Z

← Older revision		Revision as of 16:37, 31 July 2014
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	Final part of movie shows the two cells in bright field illumination.		Final part of movie shows the two cells in bright field illumination.

			{{Mitosis movie 1 links}}
			\|}

	~~'''~~Original legend~~:''' "~~A time-lapse video sequence of an EGFP-topoII-expressing GT2-LPk cell imaged from late prometaphase through telophase. Chromosomes align at the metaphase plate with EGFP-topoII concentrated at kinetochores and along the axes of the chromosome arms. As chromosomes separate at anaphase, the kinetochore concentration disappears. In late anaphase, the levels of EGFP-topo II rise inthe cytoplasm, with some concentrated into foci that are motile and, at telophase, appear to fuse with the reforming nuclei. A phase contrast image of the two daughter cells is shown at the end of the sequence. The fluorescence images in this sequence were individually scaled in order to optimize visualization of the EGFP-topoII cytoplasmic foci that appear inanaphase and telophase.		===Original legend===
			A time-lapse video sequence of an EGFP-topoII-expressing GT2-LPk cell imaged from late prometaphase through telophase. Chromosomes align at the metaphase plate with EGFP-topoII concentrated at kinetochores and along the axes of the chromosome arms. As chromosomes separate at anaphase, the kinetochore concentration disappears. In late anaphase, the levels of EGFP-topo II rise inthe cytoplasm, with some concentrated into foci that are motile and, at telophase, appear to fuse with the reforming nuclei. A phase contrast image of the two daughter cells is shown at the end of the sequence. The fluorescence images in this sequence were individually scaled in order to optimize visualization of the EGFP-topoII cytoplasmic foci that appear inanaphase and telophase.


	~~{{Mitosis movie 1 links}}~~
	\|}

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	[[Category:Movies~~]] [[Category:Flash~~]] [[Category:Mitosis]]		[[Category:Movies]] [[Category:Mitosis]]

	{{Movie footer}}		{{Movie footer}}

Z8600021 at 03:38, 31 July 2014

2014-07-31T03:38:27Z

← Older revision		Revision as of 14:38, 31 July 2014
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	{\|		{\|
	\| width=420px\|<html5media height="420" width="400">File:Mitosis 01.mp4</html5media>		\| width=420px\|<html5media height="420" width="400">File:Mitosis 01.mp4</html5media>

			[[Media:Mitosis 01.mp4\|'''Click Here''' to play on mobile device]]
	\| [[File:Mitosis 01 icon.jpg\|100px\|left]] Movie shows a cell dividing by mitosis with a fluorescently labelled protein that is located at the kinetochores and along the axes of the chromosome arms.		\| [[File:Mitosis 01 icon.jpg\|100px\|left]] Movie shows a cell dividing by mitosis with a fluorescently labelled protein that is located at the kinetochores and along the axes of the chromosome arms.

Z8600021 at 12:57, 17 Haziran 2014

2014-06-17T12:57:34Z

← Older revision		Revision as of 23:57, 17 Haziran 2014
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	{{Movie header}}		{{Movie header}}
	{\|		{\|
	\| width=420px\|<html5media height="~~400~~" width="400">File:Mitosis 01.mp4</html5media>		\| width=420px\|<html5media height="420" width="400">File:Mitosis 01.mp4</html5media>
	\| [[File:Mitosis 01 icon.jpg\|100px\|left]] Movie shows a cell dividing by mitosis with a fluorescently labelled protein that is located at the kinetochores and along the axes of the chromosome arms.		\| [[File:Mitosis 01 icon.jpg\|100px\|left]] Movie shows a cell dividing by mitosis with a fluorescently labelled protein that is located at the kinetochores and along the axes of the chromosome arms.

Z8600021 at 12:57, 17 Haziran 2014

2014-06-17T12:57:12Z

← Older revision		Revision as of 23:57, 17 Haziran 2014
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	{{Movie header}}		{{Movie header}}
	{\|		{\|
	\| width=420px\|<~~mediaplayer width~~='400~~' height='390' image~~="~~http://embryology.med.unsw.edu.au/embryology/images/3/37/Mitosis_01_icon.jpg~~">File:Mitosis 01.mp4</~~mediaplayer~~>		\| width=420px\|<html5media height="400" width="400">File:Mitosis 01.mp4</html5media>
	\| [[File:Mitosis 01 icon.jpg\|100px\|left]] Movie shows a cell dividing by mitosis with a fluorescently labelled protein that is located at the kinetochores and along the axes of the chromosome arms.		\| [[File:Mitosis 01 icon.jpg\|100px\|left]] Movie shows a cell dividing by mitosis with a fluorescently labelled protein that is located at the kinetochores and along the axes of the chromosome arms.

Z8600021 at 22:23, 6 March 2013

2013-03-06T22:23:59Z

← Older revision		Revision as of 09:23, 7 March 2013
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	<pubmed>12105179</pubmed>		<pubmed>12105179</pubmed>

			{{JCB}}

	Corresponds to Fig. 2 A. from the above paper.		Corresponds to Fig. 2 A. from the above paper.
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	Original File Name: JCB200202053.V1.mov 5 Mb http://jcb.rupress.org/content/suppl/2002/07/01/jcb.200202053.DC1/1.html		Original File Name: JCB200202053.V1.mov 5 Mb http://jcb.rupress.org/content/suppl/2002/07/01/jcb.200202053.DC1/1.html

	~~{{JCB}}~~

	[[Category:Movies]] [[Category:Flash]] [[Category:Mitosis]]		[[Category:Movies]] [[Category:Flash]] [[Category:Mitosis]]

			{{Movie footer}}

Z8600021 at 22:22, 6 March 2013

2013-03-06T22:22:01Z

← Older revision		Revision as of 09:22, 7 March 2013
Line 1:		Line 1:
	{{Movie header}}		{{Movie header}}
	{\|		{\|
	\| width=420px\|<mediaplayer width='400' height='390' image="http://embryology.med.unsw.edu.au/embryology/images/a/a9/~~Week2_001_icon~~.jpg">File:Mitosis 01.mp4</mediaplayer>		\| width=420px\|<mediaplayer width='400' height='390' image="http://embryology.med.unsw.edu.au/embryology/images/3/37/Mitosis_01_icon.jpg">File:Mitosis 01.mp4</mediaplayer>
	\| [[File:Mitosis 01 icon.jpg\|100px\|left]] Movie shows a cell dividing by mitosis with a fluorescently labelled protein that is located at the kinetochores and along the axes of the chromosome arms.		\| [[File:Mitosis 01 icon.jpg\|100px\|left]] Movie shows a cell dividing by mitosis with a fluorescently labelled protein that is located at the kinetochores and along the axes of the chromosome arms.

Z8600021: Created page with "{{Movie header}} {| | width=420px|File:Mitosis 01.mp4
2013-03-06T22:21:26Z

Created page with "{{Movie header}} {| | width=420px|<mediaplayer width='400' height='390' image="http://embryology.med.unsw.edu.au/embryology/images/a/a9/Week2_001_icon.jpg">File:Mitosis 01.mp4</media..."

New page
{{Movie header}}
{|
| width=420px|<mediaplayer width='400' height='390' image="http://embryology.med.unsw.edu.au/embryology/images/a/a9/Week2_001_icon.jpg">File:Mitosis 01.mp4</mediaplayer>
| [[File:Mitosis 01 icon.jpg|100px|left]] Movie shows a cell dividing by mitosis with a fluorescently labelled protein that is located at the kinetochores and along the axes of the chromosome arms.

The labelling allows you to see the chromosomes and the linking region (kinetochore) between chromosome pairs and the mitotic spindle microtubules.

Final part of movie shows the two cells in bright field illumination.

'''Original legend:''' "A time-lapse video sequence of an EGFP-topoII-expressing GT2-LPk cell imaged from late prometaphase through telophase. Chromosomes align at the metaphase plate with EGFP-topoII concentrated at kinetochores and along the axes of the chromosome arms. As chromosomes separate at anaphase, the kinetochore concentration disappears. In late anaphase, the levels of EGFP-topo II rise inthe cytoplasm, with some concentrated into foci that are motile and, at telophase, appear to fuse with the reforming nuclei. A phase contrast image of the two daughter cells is shown at the end of the sequence. The fluorescence images in this sequence were individually scaled in order to optimize visualization of the EGFP-topoII cytoplasmic foci that appear inanaphase and telophase.

{{Mitosis movie 1 links}}
|}

----
===Reference===
<pubmed>12105179</pubmed>

Corresponds to Fig. 2 A. from the above paper.

Original File Name: JCB200202053.V1.mov 5 Mb http://jcb.rupress.org/content/suppl/2002/07/01/jcb.200202053.DC1/1.html

{{JCB}}

[[Category:Movies]] [[Category:Flash]] [[Category:Mitosis]]