2011 Lab 1 - Revision history

S8600021 at 04:33, 27 July 2011

2011-07-27T04:33:15Z

← Older revision		Revision as of 15:33, 27 July 2011
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	This first lab class will have 2 main parts.		This first lab class will have 2 main parts.

	# An introduction to the basics of gametogenesis and [[Fertilization]].		# An introduction to the basics of gametogenesis and Fertilization.
	# An opportunity to set up your own student page and begin learning some basic editing for your individual assessment page. I will demonstrate by making a [[Test Student 2011]] page.		# An opportunity to set up your own student page and begin learning some basic editing for your individual assessment page. I will demonstrate by making a [[Test Student 2011]] page.
	==Objectives==		==Objectives==

S8600021: /* Objectives */

2011-07-27T04:19:28Z

Objectives

← Older revision		Revision as of 15:19, 27 July 2011
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	# Introduce the group assessment project.		# Introduce the group assessment project.


	~~----~~

	{{Template:2011Lab1}}		{{Template:2011Lab1}}

S8600021 at 03:46, 27 July 2011

2011-07-27T03:46:12Z

← Older revision		Revision as of 14:46, 27 July 2011
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	# Introduce the group assessment project.		# Introduce the group assessment project.


			----

			{{Template:2011Lab1}}


	{{2011ANAT2341}}		{{2011ANAT2341}}

S8600021 at 03:38, 27 July 2011

2011-07-27T03:38:22Z

Show changes

S8600021: /* Zygote Contributions */

2011-07-27T03:36:59Z

Zygote Contributions

← Older revision		Revision as of 14:36, 27 July 2011
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	# impacts upon blastocyst development		# impacts upon blastocyst development

	~~== Zygote Contributions ==~~
	~~'''Maternal''' - mitochondria, nucleolus, oocyte contributes one centriole during fertilization~~

	~~'''Paternal''' - spermatozoon contributes one centriole during fertilization~~

	~~'''Both Parents''' - chromosomes (inherent epigenetic differences between the paternal and maternal pronuclei), plasma membranes spermatozoon/oocyte mingle to form a mosaic plasma membrane~~

S8600021: /* Fertilization Overview */

2011-07-27T03:29:13Z

Fertilization Overview

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	== Fertilization Overview ==		=== Fertilization Overview ===

	=== Capacitation ===		==== Capacitation ====

	* The mammalian spermatozoa once released, must remain for a time in the female genital tract before having the capacity to fertilize the oocyte. This process involves modifying the spermatozoa.		* The mammalian spermatozoa once released, must remain for a time in the female genital tract before having the capacity to fertilize the oocyte. This process involves modifying the spermatozoa.

	=== Acrosome Reaction ===		==== Acrosome Reaction ====

	* Penetration of egg by spermatozoa is initiated by the acrosome reaction which takes different forms in different species.		* Penetration of egg by spermatozoa is initiated by the acrosome reaction which takes different forms in different species.
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	* The inner portion of the acrosomal membrane everts and lengthens to form the acrosomal tubule through which the sperm nucleus enters the egg.		* The inner portion of the acrosomal membrane everts and lengthens to form the acrosomal tubule through which the sperm nucleus enters the egg.

	=== Sperm Contact ===		==== Sperm Contact ====

	The act of fertilization changes the egg from a stage of slow structural and metabolic decline to one of renewed activation. Morphologically egg activation is a series of surface changes immediately following sperm contact.		The act of fertilization changes the egg from a stage of slow structural and metabolic decline to one of renewed activation. Morphologically egg activation is a series of surface changes immediately following sperm contact.
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	* Mammals - No phenomenon comparable to the raising of the fertilization membrane is displayed. Mammalian eggs are surrounded by the zona pellucida which undergoes a structural change known as the zonal reaction after sperm penetration. On sperm contact with the egg plasma membrane, cortical granules break down as in above forms, substances liberated into the perivitelline space rapidly modify the zona pellucida resulting in a block to further sperm penetration.		* Mammals - No phenomenon comparable to the raising of the fertilization membrane is displayed. Mammalian eggs are surrounded by the zona pellucida which undergoes a structural change known as the zonal reaction after sperm penetration. On sperm contact with the egg plasma membrane, cortical granules break down as in above forms, substances liberated into the perivitelline space rapidly modify the zona pellucida resulting in a block to further sperm penetration.

	=== Sperm Activation of Egg ===		==== Sperm Activation of Egg ====

	* During fertilization sperm activates the egg by induction of a calcium ion (Ca<sup>2+</sup>) oscillation within the egg's cytoplasm.		* During fertilization sperm activates the egg by induction of a calcium ion (Ca<sup>2+</sup>) oscillation within the egg's cytoplasm.

S8600021: /* Pronuclear Fusion */

2011-07-27T03:28:28Z

Pronuclear Fusion

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	\|}		\|}

	== Pronuclear Fusion ==		=== Pronuclear Fusion ===

	{\| border='0px'		{\| border='0px'

S8600021: /* Sperm Penetration */

2011-07-27T03:28:18Z

Sperm Penetration

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	# '''Second meiotic division''' - completion of 2nd meiotic division, forms second polar body		# '''Second meiotic division''' - completion of 2nd meiotic division, forms second polar body

	== Sperm Penetration ==		=== Sperm Penetration ===
	{\| border='0px'		{\| border='0px'

S8600021: /* Egg Events */

2011-07-27T03:27:54Z

Egg Events

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	# '''Membrane fusion''' - between sperm and egg, allows sperm nuclei passage into egg cytoplasm		# '''Membrane fusion''' - between sperm and egg, allows sperm nuclei passage into egg cytoplasm

	== Egg Events ==		=== Egg Events ===
	# '''Sperm membrane fusion''' - causes depolarization of egg membrane, primary block to polyspermy		# '''Sperm membrane fusion''' - causes depolarization of egg membrane, in non-mammalian species a primary block to polyspermy.
	# '''Cortical reaction''' - IP3 pathway elevates intracellular Calcium, exocytosis of cortical granules, enzyme alters ZP3 so it will no longer bind sperm plasma membrane ([http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=mboc4.figgrp.3743 cortical reaction])		# '''Cortical reaction''' - IP3 pathway elevates intracellular Calcium, exocytosis of cortical granules, enzyme alters ZP3 so it will no longer bind sperm plasma membrane ([http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=mboc4.figgrp.3743 cortical reaction])
	# '''Second meiotic division''' - completion of 2nd meiotic division, forms second polar body		# '''Second meiotic division''' - completion of 2nd meiotic division, forms second polar body

S8600021: /* Sperm Events */

2011-07-27T03:27:24Z

Sperm Events

← Older revision		Revision as of 14:27, 27 July 2011
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	== Sperm Events ==		=== Sperm Events ===
	# '''Capacitation''' - removal of glycoprotein coat and seminal proteins, alteration of sperm mitochondria		# '''Capacitation''' - removal of glycoprotein coat and seminal proteins, alteration of sperm mitochondria
	# '''Binding''' - ZP3 acts as receptor for sperm		# '''Binding''' - ZP3 acts as receptor for sperm