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From Embryology

2018

Course Links

Histology Glossary: A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | ANAT2241 Support | Histology | Histology Stains | Embryology Glossary
Common Histology Stains  
Histology Stains - Common Stains and Their Reactions
Stain
Nucleus
Cytoplasm
Collagen
RBCs
Other
Haematoxylin
blue
-
-
-
mucins - light blue
Eosin
-
pink
pale pink
bright red
colloid - pinkmuscle - red
Iron Haematoxylin
blue/black
-
-
-
Van Gieson
-
brown/yellow
red
yellow
muscle: yellow/browncartilage - pink
Verhoeff's Elastin
black
-
-
-
elastic fibres - black
Tartrazine
-
yellow
yellow
yellow
Silver Impregnation
-
-
grey/brown
-
reticular fibres - black
Methyl Green
dark green
light green
light green
green
Nuclear Fast Red
red
pink
pink
pink
Gomori's Trichrome
purple/red
purple
green
red
keratin - redmuscle - purple/red
Heidenhain's Azan
red
purple/red
deep blue
red
muscle - red
Osmium Tetroxide
-
-
brown
brown
myelin, lipids - black
Alcian Blue
-
-
-
-
mucins, - blue
Periodic acid-Schiff (PAS)
-
-
pink
-
mucins, glycogen, glycocalyx - magenta
Phosphotungstic Acid-Hematoxylin (PTAH)
blue
-
red
blue
muscle bands - blue
Masson's Trichrome
blue/black
red
green/blue
red
cartilage, mucins - blue or green; muscle - red
Luxol Fast Blue
-
-
-
variable
myelin - blue
Aldehyde Fuchsin
-
-
-
-
elastic fibres, mast cells - deep purple
Light Green
-
-
light green
-
Gallocyanin
dark blue
-
-
-
nucleic acids, Nissl granules - dark blue
Romanowsky (e.g. Leishman's)
blue
pink
acidophils - red, basophils - blue, azurophilic - purple
Aldehyde Pararosanilin elastic fibres - purple
Haematoxylin and Eosin
One of the most common staining techniques in pathology and histology. Acronym "H and E" stain. (H&E, HE).


Haematoxylin
  • Stains nuclei blue to dark-blue.
  • Stains the matrix of hyaline cartilage, myxomatous, and mucoid material pale blue.
  • Stains myelin weakly but is not noticeable if combined with eosin stain.
  • combined with Orange G (H & Or. G.) instead of eosin, specifically stains the granules of acidophilic cells of the adenohypophysis (anterior pituitary).
Eosin
  • Stains cytoplasm pink to red; red blood cells are also bright red.
  • Common counterstain to hematoxylin.
  • Stain intensity varies with the formula as well as the fixative.

Virtual slides

Moodle - ANAT2241 - 2018

Pages require student zpass to access.

The Virtual Microscope | Covering and Lining Epithelia | Glandular Epithelia | Connective Tissue Components | Connective Tissue Types | Bone, Bone Formation and Joints | Blood | Muscle Tissue | Nervous Tissue | Cardiovascular System | Respiratory System | Integumentary System (skin) | Liver, Gallbladder, and Pancreas | Gastro-Intestinal System I | Gastro-Intestinal System II | Lymphatic Tissue and Immune System | Endocrine System | Urinary System | Female Reproductive System | Male Reproductive System | Special Sense Organ: The Eye


Practical support

Pages can be accessed from any internet connected computer.

ANAT2241 Support Links: The Virtual Microscope | Covering and Lining Epithelia | Glandular Epithelia | CT Components | CT Types | Bone, Bone Formation and Joints | Blood | Muscle | Nervous | Cardiovascular | Respiratory | Integumentary | GIT Organs | GIT 1 | GIT 2 | Lymphatic and Immune | Endocrine | Urinary | Female Reproductive | Male Reproductive | Eye | Histology Stains | Histology Drawings | Practicals Health and Safety 2013 | Moodle - 2018


ANAT2241 This practical support page content is not part of the science practical class and provides only background information for student self-directed learning purposes.


2017

Practicals

Practicals Health and Safety 2013

| The Virtual Microscope | Covering and Lining Epithelia | Glandular Epithelia | Connective Tissue Components | Connective Tissue Types | Blood | Bone, Bone Formation and Joints | Muscle Tissue | Nervous Tissue | Cardiovascular System | Respiratory System | Integumentary System (skin) | Gastro-Intestinal System I | Gastro-Intestinal System II | Liver, Gallbladder, and Pancreas | Lymphatic Tissue and Immune System | Endocrine System | Urinary System | Female Reproductive System | Male Reproductive System | Special Sense Organ: The Eye


Virtual Slide Links Feb 2013

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/258A08 ANAT2241 Virtual Slides

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/24AD9A 1 The Virtual Microscope

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/B425A5 2 Covering and Lining Epithelia

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/673C86 3 Glandular Epithelia

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/1CBC81 4 CT Components

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/41D11E 5 CT Types

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/1D55D3 6 Blood

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/A3F666 7 Bone, Bone Formation and Joints

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/27D1DF 8 Muscle Tissue

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/7F4D99 9 Nervous Tissue

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/EC848A 10 Cardiovascular System

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/A4151E 11 Respiratory System

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/A519E0 12 Integumentary System (skin)

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/01277E 13 Gastro-Intestinal System I

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/01277E 14 Gastro-Intestinal System II

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/6E0BD4 15 Liver, Gallbladder, and Pancreas

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/A7D753 16 Lymphatic Tissue and Immune System

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/C8858C 17 Endocrine System

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/232022 18 Urinary System

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/E2CA06 19 Female Reproductive System

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/749C96 20 Male Reproductive System

http://vslides.unsw.edu.au/VirtualSlideV2.nsf/id/DB2FAF 21 Special Sense Organ: The Eye

Format

ANAT2241 Virtual Slides 1 The Virtual Microscope 2 Covering and Lining Epithelia 3 Glandular Epithelia 4 Connective Tissue Components 5 Connective Tissue Types 6 Blood 7 Bone and Joints 8 Muscle 9 Nervous 10 Cardiovascular 11 Respiratory 12 Integumentary 13 Gastro-Intestinal I 14 Gastro-Intestinal II 15 Liver, Gallbladder, and Pancreas 16 Lymphatic Tissue and Immune 17 Endocrine 18 Urinary 19 Female Reproductive 20 Male Reproductive 21 Special Sense The Eye