Talk:Gastrulation Planar Cell Movement Movie

From Embryology

Planar cell movements and oriented cell division during early primitive streak formation in the mammalian embryo

Dev Dyn. 2011 Aug;240(8):1905-16. doi: 10.1002/dvdy.22687.

Halacheva V, Fuchs M, Dönitz J, Reupke T, Püschel B, Viebahn C.


Formation of the mammalian primitive streak appears to rely on cell proliferation to a minor extent only, but compensating cell movements have not yet been directly observed. This study analyses individual cell migration and proliferation simultaneously, using multiphoton and differential interference contrast time-lapse microscopy of late pregastrulation rabbit blastocysts. Epiblast cells in the posterior gastrula extension area accumulated medially and displayed complex planar movements including U-turns and a novel type of processional cell movement. In the same area metaphase plates tended to be aligned parallel to the anterior-posterior axis, and statistical analysis showed that rotations of metaphase plates causing preferred orientation were near-complete 8 min before anaphase onset; in some cases, rotations were strikingly rapid, achieving up to 45° per min. The mammalian primitive streak appears to be formed initially with its typically minimal anteroposterior elongation by a combination of oriented cell divisions with dedicated planar cell movements. Copyright © 2011 Wiley-Liss, Inc.

PMID 21761476