Talk:ANAT2341 Lab 2
Effect of pre-in vitro maturation with cAMP modulators on the acquisition of oocyte developmental competence in cattle
J Reprod Dev. 2018 Jun 22;64(3):233-241. doi: 10.1262/jrd.2018-009. Epub 2018 Mar 2.
Sugimura S1, Yamanouchi T2, Palmerini MG3, Hashiyada Y2, Imai K4, Gilchrist RB5.
The administration of follicle-stimulating hormone (FSH) prior to oocyte retrieval improves oocyte developmental competence. During bovine embryo production in vitro, however, oocytes are typically derived from FSH-unprimed animals. In the current study, we examined the effect of pre-in vitro maturation (IVM) with cAMP modulators, also known as the second messengers of FSH, on the developmental competence of oocytes derived from small antral follicles (2-4 mm) of FSH-unprimed animals. Pre-IVM with N6,2'-O-dibutyryladenosine 3',5'-cyclicmonophosphate (dbcAMP) and 3-isobutyl-1-methylxanthine (IBMX) for 2 h improved the blastocyst formation in oocytes stimulated by FSH or amphiregulin (AREG). Furthermore, pre-IVM enhanced the expression of the FSH- or AREG-stimulated extracellular matrix-related genes HAS2, TNFAIP6, and PTGS2, and epidermal growth factor (EGF)-like peptide-related genes AREG and EREG. Additionally, pre-IVM with dbcAMP and IBMX enhanced the expression of EGFR, and also increased and prolonged cumulus cell-oocyte gap junctional communication. The improved oocyte development observed using the pre-IVM protocol was ablated by an EGF receptor phosphorylation inhibitor. These results indicate that pre-IVM with cAMP modulators could contribute to the acquisition of developmental competence by bovine oocytes from small antral follicles through the modulation of EGF receptor signaling and oocyte-cumulus/cumulus-cumulus gap junctional communication. KEYWORDS: Bovine; EGFR signaling; Embryo development; Gap junction; Pre-IVM PMID: 29503399 PMCID: PMC6021610 DOI: 10.1262/jrd.2018-009
|Associate Professor Robert Gilchrist||Dr Hayden Homer|
|Talk - The Reproductive Technology Revolution||Talk - Meiosis in Mammalian Oocytes and Age-related Vulnerability|
|Dr Gilchrist’s primary research interests are in the regulation of mammalian oocyte development and maturation, and the development of novel oocyte maturation techniques for infertility treatment.||Dr Homer leads a research programme aimed at understanding the molecular basis of oocyte quality and its striking decline with aging, especially as women go beyond their mid-thirties.|
|UNSW Research Gateway - PubMed||UNSW Research Gateway - PubMed|
Oocyte BMP15 and GDF9 effects PMID 25058588
Meiosis sister kinetochore geometry PMID 24642833
How separated sisters get bad connections
Cell Cycle. 2014 Apr 15;13(8):1222-3. doi: 10.4161/cc.28567. Epub 2014 Mar 18.
KEYWORDS: aneuploidy; cohesin; kinetochore; meiosis I; meiosis II; microtubule attachment; mouse oocyte
Comment on Kinetochore microtubule establishment is defective in oocytes from aged mice. [Cell Cycle. 2014]
2012 Lab 2
|ANAT2341 Lab 2: Introduction | Fertilization | Week 1 | Week 2 | Online Assessment | Group Project|